Polarization Colors Of Collagen Fibers Research Articles

Comparative Evaluation of Polarization Colors of Collagen Fibers in Odontogenic Keratocyst and Ameloblastoma Using Picrosirius Red Stain (Histochemical Study)

Aims: studying the quality and quantity of collagen fibers may help to delineate their influence on the aggressiveness and growth potential of the OKC and ameloblastoma. The aims of this study were to evaluate and compare the polarization colors, orientation, and organization of collagen fibers in the connective tissues of the odontogenic keratocyst (OKC), and ameloblastoma (AB) using picrosirius red (PSR) stain under a polarizing microscope with correlation to their biologic behavior.
 Materials and method: polarization colors, orientation, and organization of collagen fibers in pre-diagnosed cases of OKC and AB, twenty each, and two control cases of dentigerous cyst (DC) were histochemically analyzed using PSR stain and a polarized light microscope.
 Results: comparing the collagen fibers in the two lesions, there was no significant difference with respect to the polarization colors, orientation, and organization.
 Conclusion: PSR staining and polarizing microscopy is a powerful tool to appraise the nature and arrangement of collagen fibers in odontogenic lesions. The connective tissue of OKC showed a predominance of green-yellow birefringence, and loosely packed fibers, this can be correlated to the aggressive nature of this lesion.

A comparative study of influence of inflammation and collagen deposition in the cystic wall of infected and non-infected odontogenic keratocyst using picrosirius red and polarizing microscopy

Aim: To evaluate whether inflammation influences polarization colors of collagen fibers and to study their staining pattern in the connective tissue wall of infected and non-infected odontogenic keratocyst (OKC) using picrosirius red and polarizing microscopy. Materials and Methods: Histopathologically diagnosed 50 cases of OKC were studied. Degree of inflammation was examined on H and E stained slides and inflammatory density was graded on a 4-grade scale from 0 to 4. Cases were then divided into three groups, A to C according to the inflammatory score. Picrosirius red-stained sections were viewed under a polarized microscope for birefringence of collagen fibers and the colors for both thin [0.8μm or less] and thick [between 1.4 and 2.4 μm] fibers were noted. Polarization colors were divided into three groups: Group 1: Green, 2: Yellow and 3: Red. Analysis of variance (ANOVA) was used to find the significance of study parameters. Results: In the present study, there was no significant difference in the means of polarization color pattern of thin fibers in all the three groups. The majority of thin fibers were in range of green and yellow (~90%). Cases of Group 1 had highest mean values of thick green fibers at 27.31% and cases of Group 3 had highest mean values of thick red fibers at 56.37%. Differences in mean values of thick green, yellow and red fibers between Group 1 and Group 2 and between Group 1 and Group 3 were statistically significant. Conclusion: Cases of OKC with no or mild inflammation had majority of poorly packed fibers with green and yellow birefringence and moderate to severely inflamed cases had well-packed and thick fibers showing orange to red birefringence. Our study showed that inflammation influences polarization colors and packing of collagen fibers in the connective tissue wall of infected and non-infected OKC. Thus, the study of packing of collagen fibers in correlation with inflammation using polarizing microscopy in conjunction with picrosirius red staining can be effectively used to predict the biologic behavior of OKC.

The role of stroma in the expansion of odontogenic cysts and adenomatoid odontogenic tumor: A polarized microscopy study

Objectives:To compare the polarization colors of collagen fibers of odontogenic keratocyst (OKC), radicular cyst, dentigerous cyst, and adenomatoid odontogenic tumor (AOT) with reference to their biological behavior.Study Design:Twenty cases each of OKC, radicular cyst, dentigerous cyst, and AOT were stained with picrosirius red stain and studied under polarized light.Results:A predominance of green to greenish yellow thick fibers was noted in OKC and AOT as compared to dentigerous cyst and radicular cyst. There was no significant difference between the polarization colors of the thin fibers in all the three groups.Conclusion:The stroma of OKC and AOT consists of poorly packed or pathologic collagen and plays a role in its neoplastic behavior.

The influence of inflammation on the polarization colors of collagen fibers in the wall of odontogenic keratocyst

Epithelial-mesenchymal interactions have been found to play a role in the pathogenesis of odontogenic keratocyst (OKC). In the present study we investigated the effect of inflammation in the OKC wall on the polarization colors of Picrosirius red-stained collagen fibers. 50 cases of OKC were selected and separated into two groups according to the inflammatory intensity: those with mild-to-moderate inflammation (Group A), and those with intense inflammation (Group B). The polarization colors of the collagen fibers were recorded separately for thick and thin fibers. Polarization colors of the thin fibers were in the green- to yellow spectrum, without significant differences between the groups. However, polarization colors of the thick fibers significantly differ between the groups. In Group B, the frequency of thick fibers with green birefringence decreased significantly, whereas fibers with red polarization colors increased in frequency (4.6% and 44%, respectively) compared with Group A (12.3% and 23.6%, respectively). It can than be concluded that inflammation has an impact on the packing of collagen fibers in the connective tissue wall of OKC as reflected by their birefringence colors under polarized light. In the presence of dense inflammation, the percentage of thick fibers with green birefringence decreases, with an increase in thick fibers with red birefringence which appeared more packed.