Abstract Let $G$ be a connected complex semi-simple Lie group and ${\mathcal {B}}$ its flag variety. For every positive integer $n$, we introduce a Poisson groupoid over ${{\mathcal {B}}}^n$, called the $n$th total configuration Poisson groupoid of flags of $G$, which contains a family of Poisson sub-groupoids whose total spaces are generalized double Bruhat cells and bases generalized Schubert cells in ${\mathcal {B}}^n$. Certain symplectic leaves of these Poisson sub-groupoids are then shown to be symplectic groupoids over generalized Schubert cells. We also give explicit descriptions of symplectic leaves in three series of Poisson varieties associated to $G$.