BackgroundPrevious studies have demonstrated that the point prevalence of back pain ranges from 12% to 33% and that the lifetime prevalence of back pain ranges from 28% to 51% in adolescents. However, few studies on back pain in patients with Adolescent idiopathic scoliosis (AIS) have been conducted, and these studies had significant limitations, including a lack of comparative controls and detailed information about scoliotic deformity or pain location. This study aimed to determine whether adolescents with AIS experience back pain in specific regions. MethodsThis retrospective case-control study included 189 female adolescents with AIS who underwent corrective fusion from 2008–2020. Questionnaires on back pain and health-related quality of life (HRQOL) using the Scoliosis Research Society Outcomes Instrument-22 (SRS-22) were conducted preoperatively. The control group included 2,909 general female adolescents. ResultsThe mean Cobb angles in the main thoracic and thoracolumbar/lumbar curves were 51.4±15.3° and 40.4±12.9°. Back pain characteristics included higher point prevalence (25.9%) and lifetime prevalence (64.6%) compared to healthy controls. Adolescents with back pain showed lower scores in the pain and mental health domains of the SRS-22. Adolescents with major thoracic AIS showed more back pain in the upper and middle right back compared to adolescents with major thoracolumbar/lumbar AIS. ConclusionThe point and lifetime prevalence of back pain were definitely higher in patients with AIS, which affected their HRQOL. There was a relationship between pain around the right scapula and the right major thoracic curve with a rib hump deformity.