Contact Points Research Articles

To play or not to play, that is the question: an interview study with amateur football coaches on perceptions of pain during sports participation

Amateur football coaches play a key role in preventing, assessing and treating pain among their players, as they are often the first point of contact and may be the main...

Genotype-specific expression of uncle fester suggests a role in allorecognition education in a basal chordate.

Histocompatibility is the ability to discriminate between self and non-self tissues, and has been described in species throughout the metazoa. Despite its universal presence, histocompatibility genes utilized by different phyla are unique- those found in sponges, cnidarians, ascidians and vertebrates are not orthologous. Thus, the origins of these sophisticated recognition systems, and any potential functional commonalities between them are not understood. We are studying histocompatibility in the botryllid ascidians, members of the chordate subphylum, Tunicata, which provide a powerful model to understand both the origins and functional aspects of this process. Histocompatibility in the botryllids occurs at the tips of an extracorporeal vasculature that come into contact when two individuals grow into proximity. If compatible, the vessels will fuse, forming a parabiosis between the two individuals. If incompatible, the two vessels will reject- an inflammatory reaction that results in melanin scar formation at the point of contact, blocking anastomosis. Compatibility is determined by a single, highly polymorphic locus called the fuhc with the following rules: individuals that share one or both fuhc alleles will fuse, while those who share neither will reject. The fuhc locus encodes at least six proteins with known roles in allorecognition. One of these genes, called uncle fester, is necessary and sufficient to initiate the rejection response. Here we report the existence of genotype-specific expression levels of uncle fester, differing by up to 8-fold at the mRNA-level, and that these expression levels are constant and maintained for the lifetime of an individual. We also found that these differences had functional consequences: the expression level of uncle fester correlated with the speed and severity of the rejection response. These findings support previous conclusions that uncle fester levels modulate the rejection response, and may be responsible for controlling the variation observed in the timing and intensity of the reaction. The maintenance of genotype specific expression of uncle fester is also evidence of an education process reminiscent of that which occurs in mammalian Natural Killer (NK) cells. In turn, this suggests that while histocompatibility receptors and ligands evolve via convergent evolution, they may utilize conserved intracellular machinery to interpret binding events at the cell surface.

Feasibility of a cinematic-virtual reality training program about opioid use disorder for osteopathic medical students: a single-arm pre-post study.

Opioid use disorder (OUD) has a considerable morbidity and mortality in the United States. Healthcare providers are key points of contact for those with OUD; however, some providers may hold stigma toward OUD. Stigma toward OUD can lead to lower quality of care and more negative health outcomes. Thus, new trainings designed to reduce stigma toward OUD while increasing empathy are critical. We created a web-based cinematic virtual reality (cine-VR) training program on OUD for osteopathic medical students. The aim of this pilot study was to assess changes in stigma toward OUD and empathy before and after the online cine-VR training program on OUD. We employed a single-arm, pre- and posttest pilot study to assess changes in stigma toward OUD and empathy. Osteopathic medical students from one large medical school in the Midwest with three campuses were invited to participate in the online cine-VR training. Participants completed two surveys before and after the cine-VR training. We performed paired t tests to examine changes in stigma toward OUD and empathy scores before and after the cine-VR OUD training program. A total of 48 participants completed the training. We observed a decrease in stigma toward OUD posttraining (t=4.402, p<0.001); this change had a Cohen's d of 0.64, indicating a medium effect. We also observed an increase in participants' empathy scores posttraining (t=-2.376, p=0.023), with a Cohen's d of 0.40 signifying a small effect. Findings from this pilot study suggest that the online cine-VR training may reduce stigma toward OUD while increasing empathy. Future research employing a randomized controlled trial design with a larger, more diverse sample and a proper attention control condition is needed to confirm the effectiveness of the online cine-VR training. If confirmed, this cine-VR training may be an accessible approach to educating osteopathic medical students about OUD.

A novel role of AURKA kinase in erythroblast enucleation.

Generation of mammalian red blood cells requires the expulsion of polarized nuclei late in terminal erythroid differentiation. However, the mechanisms by which spherical erythroblasts determine the direction of nuclear polarization and maintain asymmetry during nuclear expulsion are poorly understood. Given the analogy of erythroblast enucleation to asymmetric cell division and the key role of Aurora kinases in mitosis, we sought to investigate the function of Aurora kinases in erythroblast enucleation. We found that AURKA (Aurora kinase A) is abundantly expressed in orthochromatic erythroblasts. Intriguingly, high-resolution confocal microscopy analyses revealed that AURKA co-localized with the centrosome on the side of the nucleus opposite its membrane contact point during polarization and subsequently translocated to the anterior end of the protrusive nucleus upon nuclear exit. Mechanistically, AURKA regulated centrosome maturation and localization via interaction with i-tubulin to provide polarization orientation for the nucleus. Furthermore, we identified ECT2 (epithelial cell transforming 2), a guanine nucleotide exchange factor, as a new interacting protein and ubiquitination substrate of AURKA. After forming the nuclear protrusion, AURKA translocated to the anterior end of the protrusive nucleus to directly degrade ECT2, which is partly dependent on kinase activity of AURKA. Moreover, knockdown of ECT2 rescued impaired enucleation caused by AURKA inhibition. Our findings have uncovered a previously unrecognized role of Aurora kinases in the establishment of nuclear polarization and eventual nuclear extrusion and provide new mechanistic insights into erythroblast enucleation.

Impact of Restricted Public Transport Since COVID-19 on Women in Rural India: A Qualitative Study

PurposeRailways are crucial as public transport in rural India in connecting the villages with cities. Since COVID-19, most of the trains in rural India have not stopped at small railway stations, which has impacted the mobility of the villagers. This study is conducted in Piprai, a village in North India, to explore the impact of non-halting trains since COVID-19 on young girls in rural India. Design/methodology/approachThe study adopts a qualitative research design to explore the impact of non-halting trains since COVID-19 on young girls in rural India. Qualitative data was collected from three sources. Two physical participatory workshops were conducted in Piprai in March 2021 and October 2022 with 10 and 12 participants, respectively. A WhatsApp group was formed with six girls for continuous feedback and discussions. A social activist from the same area was the point of contact and was interviewed to understand the overall condition of women in the village. FindingsThe results provide valuable insights into how the lack of public transportation since COVID-19 has affected young girls in rural India. Women's mobility has been limited, which has led to a halt in higher studies, being unable to visit hospitals regularly and meeting relatives, especially parents in different cities. Furthermore, the above reasons have led to anxiety and mental disturbance. Originality/valueThis study is among the first to explore the impact of lack of transportation since COVID-19 on women in rural India. This is the first time any such study has been conducted in the remote area of Bundelkhand, UP, India.

Epithelial cells maintain memory of prior infection with Streptococcus pneumoniae through di-methylation of histone H3

Epithelial cells are the first point of contact for bacteria entering the respiratory tract. Streptococcus pneumoniae is an obligate human pathobiont of the nasal mucosa, carried asymptomatically but also the cause of severe pneumoniae. The role of the epithelium in maintaining homeostatic interactions or mounting an inflammatory response to invasive S. pneumoniae is currently poorly understood. However, studies have shown that chromatin modifications, at the histone level, induced by bacterial pathogens interfere with the host transcriptional program and promote infection. Here, we uncover a histone modification induced by S. pneumoniae infection maintained for at least 9 days upon clearance of bacteria with antibiotics. Di-methylation of histone H3 on lysine 4 (H3K4me2) is induced in an active manner by bacterial attachment to host cells. We show that infection establishes a unique epigenetic program affecting the transcriptional response of epithelial cells, rendering them more permissive upon secondary infection. Our results establish H3K4me2 as a unique modification induced by infection, distinct from H3K4me3 or me1, which localizes to enhancer regions genome-wide. Therefore, this study reveals evidence that bacterial infection leaves a memory in epithelial cells after bacterial clearance, in an epigenomic mark, thereby altering cellular responses to subsequent infections and promoting infection.

Location and capacity allocation for emergency contact points in large-scale power outages

AbstractNowadays, industry and individuals alike are highly dependent on a reliable power supply. A large-scale power outage, commonly known as a “blackout” is caused by natural disasters, cyber attacks, technical failure, or human errors, and can lead to a variety of severe consequences. The far-reaching dynamics of blackouts can even result in the collapse of critical public service infrastructure reliant on electricity (e.g., communication, water supply, medical services, public safety). Particularly, the loss of information and communication infrastructure essential to reporting medical emergencies, and the collapse of the drinking water supply are two critical stressors for the population to cope with. One attempt to tackle this situation is to install temporary emergency contact points (ECPs) into existing infrastructure. These can be approached by the population to communicate with medical personnel and to receive drinking water. Different types of professional personnel, which is a limited resource, are required to run such ECPs. Our study introduces this tactical decision problem. We formulate it as an integer linear program for the optimal spatial allocation of ECPs, such that multiple types of human resources that are required for operating such locations can be efficiently assigned. A comprehensive numerical study, based on data of the City of Vienna, demonstrates how to reduce the walking distance of inhabitants while increasing the efficiency of resource allocation. Matrix pruning based on an enforced limit of the walking distances together with a decomposition approach is utilized to solve the considered instances.

PROGRAM AND ANALYTICAL COMPLEX OF THE INDUSTRIAL SAFETY AND HEALTH ANALYSIS SYSTEM

Aim. To investigate the quantitative characteristics of magnetic fields created during electric contact welding by various methods: contact point, arc-contact, capacitor point, contact-contact continuous and pulsating reflow. The problem being solved is the lack of necessary information on the electromagnetic safety of the specified welding methods. Methodology. A description of the proposed methodical approaches to determining the levels of magnetic fields, means of measuring them, and methods of assessing their impact on the welder's body is provided. Based on the analysis and processing of the received oscillograms and spectrograms of magnetic fields, measurements of their quantitative characteristics were performed. To determine the general level of the multifrequency magnetic field that occurs during contact welding, the proposed generalized indicator of the level of the magnetic field was used. Implications of research. It was established that during contact spot welding with a stationary machine, the magnetic field level exceeds the maximum permissible value at the workplace in the range of 50-1000 Hz at a distance of 0.3 m from the welding electrodes. During manual welding by this method, the level of the magnetic field exceeds the permissible level in the frequency ranges of 5-50, 50-1000 Hz directly near the electric cable. Condenser spot welding with direct current is characterized by exceeding the maximum allowable MP at the workplace in the high-frequency range of 1000–10000 Hz. During arc-joint welding at the workplace, exceeding the maximum allowable levels of the magnetic field was not detected. Practical implications. It is shown that the spectral composition and magnitude of the magnetic field signal is determined by the welding method itself and the initial parameters of the power sources. The obtained results can be used in the field of welding production and labor protection for the improvement of contact welding technologies and the development of measures to protect welders from the magnetic field.

Fabrication and properties of hydrogel based on itaconic anhydride modified collagen

Based on the bioconjugation engineering, the structures and properties of collagen could be optimized, which is attractive for the development of novel collagen-based biomaterials. Here, itaconic anhydride was used to modify the amino groups in collagen to prepare the itaconic anhydride modified collagen (CITA) with different grafting density. The grafting density of CITAs was detected by TNBS assay. The integrity and thermostability of triple helical structure in CITAs were revealed by CD. Hydrophilicity of CITAs was studied by isoelectric point and water contact angles analysis. The concentration of CITA solution could be improved to 30 mg/mL under physiological environment. The hydrogelation of CITA was realized by photopolymerization, based on the introduction of double bonds. The storage modulus (G′) of CITA hydrogel could be improved to 680 Pa, compared with 67 Pa of Col hydrogel. The improved hemostasis property and antibacterial property of CITAs were confirmed, and CITAs also possessed good biocompatibility. This work provided a novel artificially modified collagen with optimized properties, which could be used for 3D bioprinting and fabrication of the in situ hemostatic hydrogel system.

Challenges perceived by social workers to prevent FGM/C in Bavaria: a prospective, cross-sectional survey

BackgroundWorldwide, at least 230 million girls and women are affected by female genital mutilation/ cutting (FGM/C). FGM/C violates human rights and can cause irreparable harm and even lead to death. In 2022, more than 100,000 survivors of FGM/C lived in Germany, and more than 17,000 girls were considered at risk. Due to the increasing number, there is a need to improve the skills of professionals not only to treat FGM/C but also to prevent it, aiming to maintain or improve women’s physical and mental health. However, previous studies mostly focused on health care providers, even though other professionals such as social workers, play an important role in the provision of sexual and reproductive health (SRH) care and are often the first point of contact. Therefore, the study’s main objective was to understand challenges perceived by social workers in pregnancy counselling centres in the provision of good quality of SRH care for girls and women suffering from or endangered by FGM/C.MethodsA quantitative self-administered cross-sectional online survey was sent by e-mail in 2021 to all pregnancy counselling centers in the German federal state of Bavaria.ResultsAmong the 141 participants, 82% reported no or insufficient FGM/C knowledge and barriers to provide the best quality of care. The main findings were language obstacles (82.7%), perceived client’s fear or shame (67.9%) and cultural difficulties (45.7%). Furthermore, participants also reported a lack of competence on the professional side (29.6%). Importantly, most participants (129 of 141; 92%) expressed interest in training.ConclusionProviding comprehensive good quality sexual and reproductive health care to women and girls affected from or endangered by FGM/C is challenging. The study revealed the importance of strengthening the skills of social workers and suggested the following strategies: (1) enhancing FGM/C knowledge and skills (including specialized competences e.g., in mental health) by improving training and information material for the target group, (2) improving referral pathways and addressing deficits in the existing care system (e.g. with health or legal institutions), and (3) developing trusting relationships with cultural (or traditional) mediators to build strong community networks.

Exploring Refinement Characteristics in FeTi‐Cux Composites: A Study of Localization and Abrasion Constraints

FeTi‐Cu composites with varying Cu contents are subjected to high‐pressure torsion, and their deformation behavior is explored systematically using scanning electron microscopy, microhardness, and nanoindentation. The study identifies the limiting factors influencing the refinement during severe plastic deformation. The pronounced strength differences between phases lead to fragmentation primarily through hard‐hard (FeTi‐FeTi) contact points, promoted by homogeneous, i.e., non‐localized, and possibly turbulent material flow. These conditions are prevalent in Cu‐rich composites and during high‐temperature deformation. Conversely, Cu‐lean composites exhibit deformation localization, hindering the fragmentation process. Abrasion becomes an efficient refinement mechanism at the submicron‐/nano‐scale, particularly for composites containing higher concentrations of nanocrystalline FeTi and exhibiting homogeneous plastic deformation. Consequently, deformation localization in Cu‐lean composites inhibits both refinement mechanisms, while Cu‐rich compositions and higher temperatures result in efficient refinement but at the risk of coarsening at the nano‐scale. Refinement is localization‐limited in the former case and abrasion‐limited in the latter. Optimized processing conditions can overcome these constraints, yielding a uniform nanocomposite. This study sheds light on the intricate interplay of the mechanical properties of the respective phases in a composite, emphasizing the importance of tailored compositions and deformation conditions to optimize nanocomposites, particularly when dealing with challenging material pairings.This article is protected by copyright. All rights reserved.

A novel algorithmic approach for automatic virtual articulation to avoid dynamic interferences in dental restoration designs.

There is an ongoing debate about the benefits of the facebow and individual articulator settings in prosthodontics when compared with simpler methods. The present study aims to determine whether the implementation of novel algorithmic articulator concepts may be an alternative to avoid dynamic interference contacts during the design process of fixed posterior restorations, and to what extent the occlusal morphology of the restoration is affected. From a chairside CAD database, a total of 50 clinical patient cases documenting 61 planned fixed restorations in the posterior tooth region were selected. A common CAD software program was used for the automated knowledge-based design process. When designing the restorations, functional concepts were applied, including the pure static occlusion, the average articulation, three different articulator settings as a control, and a combination of a broad range of articulation parameters (the full range dynamic articulation). The resulting dynamic contact points were compared both visually and metrically with a monitoring software program. There is a highly significant difference in avoiding dynamic interference contacts when applying the full range dynamic articulation compared with the pure static occlusion (P < 0.001) and the average articulation (P < 0.001). Furthermore, the superimposition revealed that the surface of the restorations showed nearly no visual morphologic changes after virtually grinding-in the interpenetrating contact points. The full range dynamic articulation can be used for the design of small fixed posterior restorations to avoid most dynamic interference contacts without the need for determining individual parameters for each patient.

Evaluation of disinfection effect of high-energy pulse ultraviolet disinfection equipment in medical institution settings

Objective: To evaluate the disinfection effect of high-energy pulse ultraviolet disinfection equipment in medical institution settings. Methods: The disinfection effect was evaluated through field tests and laboratory tests. Among them, 135 high-frequency contact points were selected from nine departments in the field test. Samples were collected before and after disinfection, and the disinfection effects of 75% alcohol wipes wiping disinfection, high-energy pulse ultraviolet disinfection robot disinfection and high-energy pulse ultraviolet handheld disinfection instrument were compared. In the laboratory test, 30 infected areas of the simulated test table were exposed to vertical ultraviolet irradiation and the bacterial-killing rate before and after disinfection was calculated. Results: In the field test, the bacteria-killing rates of 75% alcohol wipes, high-energy pulse ultraviolet disinfection robot and high-energy pulse ultraviolet handheld disinfection instrument were 94.99%, 91.53% and 95.94%, respectively, and the difference was statistically significant. The disinfection effect of the high-energy pulse ultraviolet handheld disinfection instrument was better than that of the high-energy pulse ultraviolet disinfection robot (P values <0.05). In the laboratory test, the killing log value of Staphylococcus aureus and Escherichia coli on the carrier were both greater than 3.00. In the simulated field test, the killing log value of Staphylococcus aureus on the surface samples were 4.99. Conclusion: Both the high-energy pulse ultraviolet handheld disinfection instrument and the high-energy pulse ultraviolet disinfection robot have good disinfection effects, which are similar to the disinfection effects of conventional 75% alcohol wipes.

Reducing edge loading and alignment outliers with image-free robotic-assisted unicompartmental knee arthroplasty: a case controlled study

BackgroundSurvivorship of medial unicompartmental knee arthroplasty (UKA) is technique-dependent. Correct femoral-tibial component positioning associates with improved survivorship. Image-free robotic-assisted unicompartmental knee arthroplasty enables preoperative and intraoperative planning of alignment and assessment of positioning prior to execution. This study aimed to compare the radiological outcomes between robotic-assisted UKA (R-UKA) and conventional UKA (C-UKA).MethodsThis retrospective case control study involved 140 UKA (82 C-UKA and 58 R-UKA) performed at an academic institution between March 2016 to November 2020, with a mean follow-up of 3 years. Postoperative radiographs were evaluated for mechanical axis and femoral-tibial component position. Component position was measured by two methods: (1) femoral-tibial component contact point with reference to four medial-to-lateral quadrants of the tibial tray and (2) femoral-tibial component contact point deviation from the center of the tibial tray as a percentage of the tibial tray width. Baseline demographics and complications were recorded.ResultsThere was a higher mean component deviation in C-UKA compared with R-UKA using method 2 (17.2% vs. 12.8%; P = 0.007), but no difference in proportion of zonal outliers using method 1 (4 outliers in C-UKA, 5.1% vs. 1 outlier in R-UKA, 1.8%; P = 0.403). R-UKA showed no difference in mean mechanical alignment (C-UKA 5° vs. R-UKA 5°; P = 0.250). 2-year survivorship was 99% for C-UKA and 97% for R-UKA. Mean operative time was 18 min longer for R-UKA (P < 0.001).ConclusionImage-free robotic-assisted UKA had improved component medio-lateral alignment compared with conventional technique.

Just a head knock? Emergency physicians need to get serious about concussion.

Attention and awareness regarding concussion injury in Australia have significantly increased in the last decade. Although most of this increase is because of discussion regarding concussions from sporting endeavours, the majority of concussions are from non-sport environments including motor vehicle crashes, workplace incidents, falls, accidents, assault and intimate partner violence. In all cases, hospital EDs are the first point of contact, yet as argued in our Opinion here, there are concerns regarding the consistency of care protocols, because of a number of reasons, as well as management and follow-up clinical practices. Our Opinion is to provide a constructive discussion as well as calling for ACEM to support research to provide evidence-based data. Finally, we provide some recommendations that could be implemented immediately to improve clinical practice for presentations of concussion injuries in EDs.

Point Contact EHL and Wear in Intermittent Motion

Abstract Intermittent motion is a complex process that involves constant speed, deceleration, static stages and acceleration. Theoretical analysis suggests that shortening the period of intermittent motion can increase the film thickness during static stages, thereby extending the life of the part. Currently, an increasing number of studies are focusing on small oscillatory movements or vibrations. However, the impact of intermittent motion cycles on the film thickness and wear in the contact area still needs to be investigated. Optical interference and acoustic emission (AE) were employed as experimental methods to investigate simple sliding point contact intermittent motion. The lubrication state transition of full film-starvation-wear in the contact area was observed and the experimental results confirmed the correctness of the elastohydrodynamic lubrication (EHL) theoretical analysis. Additionally, the regularity of starvation and AE signal change with time during intermittent motion were summarized. An in-depth analysis of the reasons why intermittent motion with a short period generates less wear was performed. This analysis provides novel ideas to reduce wear of intermittent motion mechanisms. Overall, this research contributes to the understanding of the wear during intermittent motion and provides essential insights for wear reduction in this area.

Locomotion analysis of a crawling wave robot in circular canal

In this paper, we analyze the locomotion of a wave robot crawling in a curved canal between rigid walls. We determine the conditions for crawling, considering the robot geometry, orientation, wall curvature, canal width, and friction coefficients. Initially, we present an analytical model of the different cases of crawling and conditions for advancement. We found four cases of crawling: one where the robot touches only the outer wall, two with one contact point each on the outer and inner walls, and a third where the robot has two contact points with the outer wall and one with the inner wall. Subsequently, we introduce a multibody numerical simulation, which accounts for sliding along the wall and over the ground surface. The simulation allows one to validate our analytical results and analyze the robot's behavior under different conditions. Finally, we present validating results of multiple experiments conducted with our SAW robot. This analysis encompasses all types of crawling robots with internal thrust mechanisms that are not based on contact with the side walls of the pipe or canal.

Exploring skid resistance over time: Steel slag as a pavement aggregate—comparative study and morphological analysis

Steel slag has been investigated as a potential aggregate for pavement-wearing courses in road engineering. The volume stability, resistance to high and low temperatures, and water stability for asphalt mixtures containing steel slag have been widely studied. As for the skid resistance, previous studies have primarily focused on the initial performance. The relationship between the steel slag's appearance morphology and skid resistance during the wearing process remains to be further explored. It is essential to undertake a comparative study on the long-term skid resistance of steel slag and conventional aggregates to address the differences. This research picked two steel slags and two types of conventional aggregates (granite and limestone) as research samples. The chosen aggregate samples underwent various degrees of wear treatment with the Los Angeles abrasion machine, and their angularity was examined utilizing an aggregate image measurement system (AIMS). Based on the polishing pre-treatment, the micro-texture of the aggregates was observed with the assistance of a laser confocal microscope, and its correlation with skid resistance was determined. British pendulum number (BPN) tests were also conducted and analyzed at various polishing durations. The results show that steel slag has a low abrasion rate and higher initial angularity and abrasion resistance compared to natural stones. The micro-texture analysis demonstrates that steel slags have a coarser roughness, more contact points, and more durable abrasion resistance. Additionally, the fluctuations in BPN further prove that steel slag has superior initial and sustained skid resistance. Overall, steel slag can be a promising alternative for pavement-wearing course aggregate due to its superior long-term skid resistance.

Suzanne Labin: socialist, anti-communist and ‘Globalist’

AbstractThis article follows the transnational career of Suzanne Labin, a French socialist and anti-communist professional, in order to analyse the roles and agencies of individuals while the global connectivity of anti-communist actors was growing during the ‘long 1960s’ (approximately 1955–1980). Retrospective analysis, as well as accounts from contemporaries, describes this period as a time of multi-polarisation and globalisation. I will chart Labin’s path from anti-Stalinist circles in France to conservative circles in the US, to finally becoming the first and only female permanent member of the global anti-communist umbrella organisation, the World Anti-Communist League (WACL), chairing its French chapter in 1972. An analysis of Labin’s seemingly ambiguous alliances as a socialist with anti-communist and right-wing organisations will shed light on the particularities of international anti-communism during the ‘long 1960s’ on three levels. First, it will show the dynamics between anti-communist networks and actors on national, transatlantic and global levels, especially their contact points. Second, it will underline the diversity of anti-communism and its capacity to integrate several (even contradictory) ideologemes. Third, by following Labin’s global anti-communist engagement within the WACL and her cosmopolitan lifestyle, this article will argue that anti-communist actors were actively involved in the process of globalisation during the ‘long 1960s’.

Reliable Determination of Contact Angle from the Liquid Fence.

Traditional methods of measuring contact angles often face difficulties in precisely defining the three-phase contact points. A novel method for accurately defining three-phase contact points based on liquid fences is proposed in this article. The tested liquid is placed in a cubic liquid fence composed of a pair of narrow strips of the tested solid and a pair of transparent wide strips. The transparent wide strip serves as the measurement window, and the surface tension of the liquid on it can be almost ignored. In experimental measurements, the horizontal coordinates of the end points of the liquid profile are fixed by the liquid fence, thus determining the horizontal coordinates of the three-phase contact points, which helps to accurately survey the contact angle. Additionally, since the parameters of the liquid fence are known, the size of the contact angle can also be calculated by measuring the height of the liquid level dome inside the liquid fence. Using the electric wetting effect as an example, we experimentally extracted a series of liquid contour maps with circular tops and square columns under varying voltages. The relationship curve between contact angle and voltage variation was obtained using the above methods, and a contact angle variation tendency seemed to agree well with the theoretical value.