Pocillopora Damicornis Research Articles

Symbiodiniaceae algal symbionts of Pocillopora damicornis larvae provide more carbon to their coral host under elevated levels of acidification and temperature

Symbiodiniaceae algal symbionts of Pocillopora damicornis larvae provide more carbon to their coral host under elevated levels of acidification and temperature

High diversity and abundance of fish and coral in an artificial breakwater in Colombo, Sri Lanka

The diversity of reef fishes and the diversity and abundance of corals in artificial breakwaters were studied between June and August 2023 in Port City, Colombo in the Western Province of Sri Lanka. Four methods were used: visual census using video, photographs, stationary point count and environmental DNA (eDNA). A total of 137 fish species, 74 coral species and 175 other species were recorded using the four methods. There were similarities and differences between the methods for recording fish, corals and other species. Only three fish species: Moon wrasse (Thalassoma lunare); Yellowtail fusilier (Caesio cuning) and Diamond fish (Monodactylus argenteus) were recorded by all methods. The highest number of fish species was recorded by video, followed by photographs, eDNA and stationary point count in the sequence. Coral cover ranged from 9 to 50.8% and mean live coral varied from 24.4% to 34.5 ± 5.2%. The dominant coral species was Pocillopora damicornis. Rare observations of coral bleaching (n=1) and frequent observations of marine debris (n=20) and coral damage (n=25) were recorded. Species detection using eDNA is a biomonitoring tool that did not add significantly to the list of fish and corals, but included many microscopic groups such as bacteria, diatoms and ciliates that were not recorded using other methods, and provided new ecological insights into marine systems. This is the first use of multiple monitoring tools to identify and compare methods for the biodiversity of marine fish, coral and other species in Sri Lankan marine waters.

Bleached Coral Supports High Diversity and Heterogeneity of Bacterial Communities: Following the Rule of the ‘Anna Karenina Principle’

Coral-associated bacteria are sensitive to the health status of coral and proven biomarker(s) of the coral bleaching. However, whether coral specificity or health status play a key role when coral-associated bacteria responding to coral bleaching is not known. Therefore, the bacterial communities of five species of healthy and bleached corals, Acropora millepora, Favites abdita, Galaxea fascicularis, Dipsastraea speciosa and Pocillopora damicornis, were collected along the coast of Sanya, South China Sea and targeted for associated bacterial studies. The relative abundance of the dominant class Gammaproteobacteria tended to be higher in healthy corals, while Alphaproteobacteria were more abundant in bleached corals. Dominant genus Achromobacter demonstrated higher relative abundance in healthy corals (0.675) than in bleached corals (0.151). Most of the bleached corals had high α diversity, β dispersion, heterogeneity and complexity of the co-occurrence network of bacterial communities, which support the ‘Anna Karenina Principle (AKP)’ of diverse in threatened objects and conserved in healthy ones. The bacterial communities in the bleached corals were mostly involved in the selection process, and communities in the healthy corals were involved in the undominated process, which is obtained based on the null model test of β nearest-taxon-index (βNTI) and Bray–Curtis-based Raup–Crick (RCBray). This evidence further confirmed the AKP and revealed that the bacterial communities in the bleached corals were driven by deterministic factors. These findings provide valuable insights into the connection between bacterial and coral status, and the application of the AKP in the changing patterns of bacterial communities during coral bleaching.

Potential adaptation of scleractinian coral Pocillopora damicornis during hypo-salinity stress caused by extreme pre-flood rainfall over south China

Global warming intensifies the water cycle, resulting in significant increases in precipitation and river runoff, which brings severe hypo-salinity stress to nearshore coral reefs. Ecological investigations have found that some corals exhibit remarkable adaptability to hypo-salinity stress during mass-bleaching events. However, the exact cause of this phenomenon remains unclear. To elucidate the potential molecular mechanism leading to high tolerance to hypo-salinity stress, Pocillopora damicornis was used as a research object in this study. We compared the differences in transcriptional responses and symbiotic microbiomes between bleaching and unbleaching P. damicornis during hypo-salinity stress caused by extreme pre-flood rainfall over South China in 2022. The results showed that: (1) Under hypo-salinity stress, the coral genes related to immune defense and cellular stress were significantly upregulated in bleaching corals, indicating more severe immune damage and stress, and the Symbiodiniaceae had no significant gene enrichment. Conversely, metabolic genes related to glycolysis/gluconeogenesis were significantly downregulated in unbleaching corals, whereas Symbiodiniaceae genes related to oxidative phosphorylation were significantly upregulated to meet the energy requirements of coral holobiont; (2) C1d was the dominant Symbiodiniaceae subclade in all samples, with no significant difference between the two groups; (3) The symbiotic bacterial community structure was reorganized under hypo-salinity stress. The abundance of opportunistic bacteria increased significantly in bleaching coral, whereas the relative abundance of probiotics was higher in unbleaching coral. This may be due to severe immune damage, making the coral more susceptible to opportunistic infection and bleaching. These results suggest that long-term hypo-salinity acclimation in the Pearl River Estuary enhances the tolerance of some corals to hypo-salinity stress. Corals with higher tolerance may reduce energy consumption by slowing down their metabolism, improve the energy metabolism of Symbiodiniaceae to meet the energy requirements of the coral holobiont, and alter the structure of symbiotic bacterial communities to avoid bleaching.

Biofilm-forming bacteria associated with corals secrete melanin with UV-absorption properties.

Corals are colonized by a plethora of microorganisms, and their diversity plays a significant role in the health and resilience of corals when they face oxidative stress leading to bleaching. In the current study, we examined 238 bacteria isolated from five different coral species (Acropora hyacinthus, Pocillopora damicornis, Podabacea crustacea, Porites lobata, and Pavona venosa) collected from the coral reef ecosystems of Kavaratti, Lakshadweep Islands, India. We found that bacteria such as Psychrobacter sp., Halomonas sp., Kushneria sp., Staphylococcus sp., Bacillus sp., Brachybacterium sp., Citrobacter sp., and Salinicola sp. were commonly present in the corals. On the other hand, Qipengyuania sp., Faucicola sp., Marihabitans sp., Azomonas sp., Atlantibacter sp., Cedecea sp., Krasalinikoviella sp., and Aidingimonas sp. were not previously reported from the corals. Among the bacterial isolates, a significant number showed high levels of biofilm formation (118), UV absorption (119), and melanin production (127). Considering these properties, we have identified a combination of seven bacteria from the genera Halomonas sp., Psychrobacter sp., Krasalinikoviella sp., and Micrococcus sp. as a potential probiotic consortium for protecting corals from oxidative stress. Overall, this study provides valuable insights into the coral microbiome and opens up possibilities for microbiome-based interventions to protect these crucial ecosystems in the face of global environmental challenges.

Alleviating Coral Thermal Stress via Inoculation with Quorum Quenching Bacteria.

In the background of global warming, coral bleaching induced by elevated seawater temperature is the primary cause of coral reef degradation. Coral microbiome engineering using the beneficial microorganisms for corals (BMCs) has become a hot spot in the field of coral reef conservation and restoration. Investigating the potential of alleviating thermal stress by quorum quenching (QQ) bacteria may provide more tools for coral microbial engineering remediation. In this study, QQ bacteria strain Pseudoalteromonas piscicida SCSIO 43740 was screened among 75 coral-derived bacterial strains, and its quorum sensing inhibitor (QSI) compound was isolated and identified as 2,4-di-tert-butylphenol (2,4-DTBP). Then, the thermal stress alleviating potential of QQ bacteria on coral Pocillopora damicornis was tested by a 30-day controlled experiment with three different treatments: control group (Con: 29°C), high temperature group (HT: 31°C), and the group of high temperature with QQ bacteria inoculation (HTQQ: 31°C + QQ bacteria). The results showed that QQ bacteria SCSIO 43740 inoculation can significantly mitigate the loss of symbiotic algae and impairment of photosynthesis efficiency of coral P. damicornis under thermal stress. Significant difference in superoxide dismutase (SOD) and catalase (CAT) enzyme activities between HT and HTQQ was not observed. In addition, QQ bacteria inoculation suppressed the coral microbial community beta-dispersion and improved the stability of microbial co-occurrence network under thermal stress. It was suggested that QQ bacteria inoculation can alleviate coral thermal stress via reshaping microbial interaction and maintain community stability of coral microbiome. This study provided new evidence for the probiotic function of QQ bacteria in corals, which shedding light on the development of new microbiological tools for coral reef conservation.

A mathematical model for wound healing in the reef-building coral Pocillopora damicornis

Coral reefs, among the most diverse ecosystems on Earth, currently face major threats from pollution, unsustainable fishing practices , and perturbations in environmental parameters brought on by climate change. Corals also sustain regular wounding from other sea life and human activity. Recent reef restoration practices have even involved intentional wounding by systematically breaking coral fragments and relocating them to revitalize damaged reefs, a practice known as microfragmentation. Despite its importance, very little research has explored the inner mechanisms of wound healing in corals. Some reef-building corals have been observed to initiate an immunological response to wounding similar to that observed in mammalian species. Utilizing prior models of wound healing in mammalian species as the mathematical basis, we formulated a mechanistic model of wound healing, including observations of the immune response and tissue repair in scleractinian corals for the species Pocillopora damicornis. The model consists of four differential equations which track changes in remaining wound debris, number of cells involved in inflammation, number of cells involved in proliferation, and amount of wound closure through re-epithelialization. The model is fit to experimental wound size data from linear and circular shaped wounds on a live coral fragment. Mathematical methods, including numerical simulations and local sensitivity analysis, were used to analyze the resulting model. The parameter space was also explored to investigate drivers of other possible wound outcomes. This model serves as a first step in generating mathematical models for wound healing in corals that will not only aid in the understanding of wound healing as a whole, but also help optimize reef restoration practices and predict recovery behavior after major wounding events.

Marine macroalgae and their associated bacterial communities affect larval settlement and survivorship of the coral Pocillopora damicornis

Macroalgae play crucial roles as major habitat-forming organisms in marine ecosystems, having significant impacts on coral recruitment and reef recovery. However, the interactions between marine macroalgae and coral larvae remain poorly understood. Furthermore, little is known whether differences in bacterial assemblages associated with macroalgae may play roles in this process. Here, we comprehensively investigated the impacts of different macroalgae and their associated microbiomes on larval settlement and survival of coral Pocillopora damicornis. The results revealed significant variations in larval settlement and survival rates when exposed to different macroalgal species. The highest settlement rate, reaching 90%, was observed in the presence of the red alga Hypnea pannosa, followed by green algae Caulerpa serrulata, C. racemosa, and brown algae Turbinaria gracilis, Sargassum polycystum. Correspondingly, similarities in bacterial compositions were observed between H. pannosa and C. racemosa, as well as between T. gracilis and S. polycystum, implying associated bacterial may be related with the algal functions. Furthermore, macroalgae that facilitate larval settlement exhibited higher abundances of amplicon sequence variants (ASVs) associated with the metabolism of dimethylsulfoniopropionate or the antagonism of known coral pathogens. However, the brown alga Padina boryana failed to induce larval settlement with survival rate of zero after 120 h. The algal species harbored more abundances of ASVs related to Rhizobiaceae. These findings highlight the significant impact of macroalgae and their associated microbiomes on coral recruitment, as they influence both larval settlement and survival rates.

Population genetics assessment of two pocilloporid coral species from the northern red sea: Implications for urbanized reef sustainability

Understanding the genetic makeup of key coral species is vital for effective coral reef management, as heightened genetic diversity directly influences long-term survival and resilience against environmental changes. This study focused on two widespread Indo-Pacific branching corals, Pocillopora damicornis (referred as Pocillopora cf. damicornis (as identified only morphologically) and Seriatopora hystrix, by genotyping 222 and 195 colonies, respectively, from 10 sites in the northern Gulf of Eilat, Red Sea, using six and five microsatellite markers, respectively. Both species exhibited low observed heterozygosity (0.47 for P. cf. damicornis, 0.32 for S. hystrix) and similar expected heterozygosity (0.576 for P. cf. damicornis, 0.578 for S. hystrix). Pocillopora cf. damicornis showed minimal deviations from Hardy-Weinberg equilibrium (HWE) and low but positive F values, indicating high gene flow, while S. hystrix exhibited higher diversion from HWE and positive F values, suggesting isolation by distance and possible non-random mating or genetic drift. As the Gulf of Eilat undergoes rapid urbanization, this study highlights the anthropogenic impacts on the population genetics of key ecosystem engineering species and emphasizes the importance of managing genetics of Marine Protected Areas while implementing active coral reef restoration. The differences in reproductive traits between the two species (S. hystrix being a brooder, while P. cf. damicornis a broadcast spawner), underscore the need for sustainable population genetics management of the coral reefs for the future and resilience of the coral reef ecosystem of the northern Red Sea region.

Microbial nitrogen removal in reef-building corals: A light-sensitive process

Scleractinian corals are the main framework-building groups in tropical coral reefs. In the coral holobiont, nitrogen-cycling mediated by microbes is fundamental for sustaining the coral reef ecosystems. However, little direct evidence characterizing the activities of microbial nitrogen removal via complete denitrification and anaerobic ammonium oxidation (anammox) in stony corals has been presented. In this study, multiple incubation experiments using 15N-tracer were conducted to identify and characterize N2 production by denitrification and anammox in the stony coral Pocillopora damicornis. The rates of denitrification and anammox were recorded up to 0.765 ± 0.162 and 0.078 ± 0.009 nmol N2 cm−2 h−1 respectively. Denitrification contributed the majority (∼90%) of N2 production by microbial nitrogen removal in stony corals. The microbial nitrogen removal activities showed diel rhythms, which might correspond to photosynthetic oxygen production. The N2 production rates of anammox and denitrification increased with incubation time. To the authors' knowledge, this study is the first to confirm and characterize the activities of complete denitrification and anammox in stony corals via stable isotope techniques. This study extends the understanding on nitrogen-cycling in coral reefs and how it participates in corals’ resilience to environmental stressors.

Short-term thermal acclimation improved the thermal tolerance of three species of scleractinian corals in the South China Sea

Investigating the potential and mechanisms of thermal adaptability in scleractinian corals is critical for aiding corals in dealing with global warming and improving the efficiency of coral reef restoration efforts. Although thermal acclimation facilitates the resistance of branching corals to thermal stress, little is known about the physiological processes of massive and platy corals in the South China Sea or their ability to adapt to heat stress. In this study, we conducted indoor short-term thermal acclimation simulation experiments on three types of corals: massive Porites lutea, platy Pavona decussata, and branching Pocillopora damicornis. Subsequently, we studied the responses and adaptive mechanisms of the three corals to heat stress and explored the effects of short-term thermal acclimation using physiological and biochemical markers. The results showed that the three corals exhibited comparable phenotypic and physiological responses to heat stress, including tentacle retraction and decreases in zooxanthellae density, maximum quantum efficiency of photosystem II, and glutathione content, along with increases in antioxidant activity (catalase and superoxide dismutase), ammonium assimilation (glutamine synthetase), and apoptosis (lipid peroxide and caspase-3). Additionally, heat tolerance differed among the different species of coral. Furthermore, the physiological markers performed better in all three coral types after acclimation, and the effects of short-term acclimation decreased from P. lutea to P. damicornis to P. decussata. We propose that short-term thermal acclimation enhances the heat tolerance of corals by affecting their metabolism and antioxidant capacity. Leveraging short-term thermal acclimation in coral reef restoration efforts could help corals adapt to the threats posed by global warming and enhance restoration efficiency.

Poleward migration of tropical corals inhibited by future trends of seawater temperature and calcium carbonate (CaCO3) saturation

Poleward range expansion of marine organisms is commonly attributed to anthropogenic ocean warming. However, the extent to which a single species can migrate poleward remains unclear. In this study, we used molecular data to examine the current distribution of the Pocillopora damicornis species complex in Taiwan waters and applied niche modeling to predict its potential range through the end of the 21st Century. The P. damicornis species complex is widespread across shallow, tropical and subtropical waters of the Indo-Pacific regions. Our results revealed that populations from subtropical nonreefal coral communities are P. damicornis, whose native geographical ranges are approximately between 23°N and 35°N. In contrast, those from tropical reefs are P. acuta. Our analysis of 50 environmental data layers demonstrated that the concentrations of CaCO3 polymorphs had the greatest contributions to the distributions of the two species. Future projections under intermediate shared socioeconomic pathways (SSP) 2–4.5 and very high (SSP5–8.5) scenarios of greenhouse gas emissions showed that while sea surface temperature (SST) isotherms would shift northwards, saturation isolines of two CaCO3 polymorphs, calcite (Ωcal) and aragonite (Ωarag), would shift southwards by 2100. Subsequent predictions of future suitable habitats under those conditions indicated that distinct delimitation of geographical ranges for the two species would persist, and neither would extend beyond its native geographical zones, indicating that tropical Taiwan waters are the northern limit for P. acuta. In contrast, subtropical waters are the southern limit for P. damicornis. We concluded that the decline in CaCO3 saturation would make high latitudes less inhabitable, which could be one of the boundary elements that limit poleward range expansion driven by rising SSTs and preserve the latitudinal diversity gradient (LDG) on Earth. Consequently, poleward migration of tropical reef corals to cope with warming oceans should be reevaluated.

Coral mariculture using abandoned abalone farming ponds in northeastern Taiwan

Stony corals are ecologically and economically important marine organisms. Coral aquaculture is essential to provide live coral materials for the marine ornamental trade, reef restoration programs, and environmental education. This study sought to establish coral farms in Taiwan. To culture corals, we adopted an abandoned abalone farming pond built along the northeastern coast of Taiwan, and investigated whether it is possible to propagate corals in this artificial environment. Stainless steel underwater tables were anchored to the pond floor, and fragments of Acropora tumida, Pocillopora damicornis, Stylophora pistillata, Favites pentagona, and Porites lobata were cultured on the tables for one year. Environmental factors in the pond were also monitored. The mean surface temperature was 23.07 °C, ranging from >30 °C in summer to 18 °C in winter. The mean salinity and pH were about 35 ppt and 8.14, respectively. Survivorship of these five species after one year ranged from 100% to 67%, with a 1.37–1.51-fold increase in weight and a 3.22–3.38-fold increase in area, demonstrating that although environmental factors fluctuate depending on the season and weather, long-term and stable asexual propagation of corals is feasible in the pond. For further optimization of culture conditions, the effects of initial fragment size for culture on subsequent survival and growth were investigated. Fragments of 3 sizes (1.5, 3.0, and 4.5 cm) were prepared from parental colonies of A. tumida, P. damicornis, and S. pistillata and cultured for 6 months by hanging them under the table. There were no significant differences in survivorship among these sizes. Differences were observed in biomass (weight) increase. In A. tumida, 3-cm fragments showed significantly more weight gain than other lengths, while 1.5-cm fragments showed a significant weight increase in P. damicornis. In S. pistillata, no significant difference in weight increase was observed among size groups, suggesting the necessity to optimize the initial fragment size for culture for each species before mass culture. Histological analysis showed that mature gametes were formed in some cultured corals, suggesting that sexual propagation may also be possible in the future. The established coral farm, which is culturing >4000 coral fragments now, will be able to support not only basic and applied coral research, but also sustainable reef development and marine ornamental trade.

A novel biosensing strategy on the dynamic and on-site detection of Vibrio coralliilyticus eDNA for coral health warnings

Heat stress and coral diseases are the predominant factors causing the degradation of coral reef ecosystems. Over recent years, Vibrio coralliilyticus was identified as a temperature-dependent pathogen causing tissue lysis in Pocillopora damicornis and one of the primary pathogens causing bleaching and mortality in other corals. Yet current detection techniques for V. coralliilyticus rely primarily on qPCR and ddPCR, which cannot meet the requirements for non-invasive and real-time detection. Herein, we developed an effective electrochemical biosensor modified by an Au-MoS2/rGO (AMG) nanocomposites and a specific capture probe to dynamically detect V. coralliilyticus environment DNA (eDNA) in aquarium experiments, with a lower limit of detection (0.28 fM) for synthetic DNA and a lower limit of quantification (9.8 fg/µL, ∼0.86 copies/µL) for genomic DNA. Its reliability and accuracy were verified by comparison with the ddPCR method (P > 0.05). Notably, coral tissue started to lyse at only 29 °C when the concentration of V. coralliilyticus increased abruptly to 880 copies/µL, indicating the biosensor could reflect the types of pathogen and health risks of corals under heat stress. Overall, the novel and reliable electrochemical biosensing technology provides an efficient strategy for the on-site monitoring and early warning of coral health in the context of global warming.

Life-stage specificity and temporal variations in transcriptomes and DNA methylomes of the reef coral Pocillopora damicornis in response to thermal acclimation

Understanding the acclimation capacity of reef corals across generations to thermal stress and its underlying molecular underpinnings could provide insights into their resilience and adaptive responses to future climate change. Here, we acclimated adult brooding coral Pocillopora damicornis to high temperature (32 °C vs. 29 °C) for three weeks and analyzed the changes in phenotypes, transcriptomes and DNA methylomes of adult corals and their brooded larvae. Results showed that although adult corals did not show noticeable bleaching after thermal exposure, they released fewer but larger larvae. Interestingly, larval cohorts from two consecutive lunar days exhibited contrasting physiological resistance to thermal stress, as evidenced by the divergent responses of area-normalized symbiont densities and photochemical efficiency to thermal stress. RNA-seq and whole-genome bisulfite sequencing revealed that adult and larval corals mounted distinct transcriptional and DNA methylation changes in response to thermal stress. Remarkably, larval transcriptomes and DNA methylomes also varied greatly among lunar days and thermal treatments, aligning well with their physiological metrics. Overall, our study shows that changes in transcriptomes and DNA methylomes in response to thermal acclimation can be highly life stage-specific. More importantly, thermally-acclimated adult corals could produce larval offspring with temporally contrasting photochemical performance and thermal resilience, and such variations in larval phenotypes are associated with differential transcriptomes and DNA methylomes, and are likely to increase the likelihood of reproductive success and plasticity of larval propagules under thermal stress.

Corals nitrogen and carbon isotopic signatures alters under Artificial Light at Night (ALAN)

This study examines the impact of Artificial Light at Night (ALAN) on two coral species, Acropora eurystoma and Pocillopora damicornis, in the Gulf of Aqaba/Eilat Red Sea, assessing their natural isotopic responses to highlight changes in energy and nutrient sourcing due to sensory light pollution. Our findings indicate significant disturbances in photosynthetic processes in Acropora eurystoma, as evidenced by shifts in δ13C values under ALAN, pointing to alterations in carbon distribution or utilization. In Pocillopora damicornis, similar trends were observed, with changes in δ13C and δ15N values suggesting a disruption in its nitrogen cycle and feeding strategies.The study also uncovers species-specific variations in heterotrophic feeding, a crucial factor in coral resilience under environmental stress, contributing to the corals' fixed carbon budget. Light measurements across the Gulf demonstrated a gradient of light pollution which possess the potential of affecting marine biology in the region. ALAN was found to disrupt natural diurnal tentacle behaviors in both coral species, crucial for prey capture and nutrient acquisition, thereby impacting their isotopic composition and health.Echoing previous research, our study underscores the need to consider each species' ecological and physiological contexts when assessing the impacts of anthropogenic changes. The findings offer important insights into the complexities of marine ecosystems under environmental stress and highlight the urgency of developing effective mitigation strategies.

Bacteria undergo significant shifts while archaea maintain stability in Pocillopora damicornis under sustained heat stress

Global warming reportedly poses a critical risk to coral reef ecosystems. Bacteria and archaea are crucial components of the coral holobiont. The response of archaea associated with warming is less well understood than that of the bacterial community in corals. Also, there have been few studies on the dynamics of the microbial community in the coral holobiont under long-term heat stress. In order to track the dynamic alternations in the microbial communities within the heat-stressed coral holobiont, three-week heat-stress monitoring was carried out on the coral Pocillopora damicornis. The findings demonstrate that the corals were stressed at 32 °C, and showed a gradual decrease in Symbiodiniaceae density with increasing duration of heat stress. The archaeal community in the coral holobiont remained relatively unaltered by the increasing temperature, whereas the bacterial community was considerably altered. Sustained heat stress exacerbated the dissimilarities among parallel samples of the bacterial community, confirming the Anna Karenina Principle in animal microbiomes. Heat stress leads to more complex and unstable microbial networks, characterized by an increased average degree and decreased modularity, respectively. With the extension of heat stress duration, the relative abundances of the gene (nifH) and genus (Tistlia) associated with nitrogen fixation increased in coral samples, as well as the potential pathogenic bacteria (Flavobacteriales) and opportunistic bacteria (Bacteroides). Hence, our findings suggest that coral hosts might recruit nitrogen-fixing bacteria during the initial stages of suffering heat stress. An environment that is conducive to the colonization and development of opportunistic and pathogenic bacteria when the coral host becomes more susceptible as heat stress duration increases.

Physical and cellular impact of environmentally relevant microplastic exposure on thermally challenged Pocillopora damicornis (Cnidaria, Scleractinia)

Microplastic pollution is an increasing threat to coral reefs, which are already strongly challenged by climate change-related heat stress. Although it is known that scleractinian corals can ingest microplastic, little is known about their egestion and how microplastic exposure may impair corals at physiological and cellular levels. In addition, the effects of microplastic pollution at current environmental concentration have been little investigated to date, particularly in corals already impacted by heat stress. In this study, the combined effects of these environmental threats on Pocillopora damicornis were investigated from a physical and cellular perspective. Colonies were exposed to three concentrations of polyethylene microplastic beads (no microplastic beads: [No MP], 1 mg/L: [Low MP]; 10 mg/L: [High MP]), and two different temperatures (25 °C and 30 °C) for 72 h. No visual signs of stress in corals, such as abnormal mucus production and polyp extroflection, were recorded. At [Low MP], beads adhered to colonies were ingested but were also egested. Moreover, thermally stressed colonies showed a lower adhesion and higher egestion of microplastic beads. Coral bleaching was observed with an increase in temperature and microplastic bead concentration, as indicated by a general decrease in chlorophyll concentration and Symbiodiniaceae density. An increase in lipid peroxidation was measured in colonies exposed to [Low MP] and [High MP] and an up-regulation of stress response gene hsp70 was observed due to the synergistic interaction of both stressors. Overall, our findings showed that heat stress still represents the main threat to P. damicornis, while the effect of microplastics on coral health and physiology may be minor, especially at control temperature. However, microplastics could exacerbate the effect of thermal stress on cellular homeostasis, even at [Low MP]. While reducing ocean warming is critical for preserving coral reefs, effective management of emerging threats like microplastic pollution is equally essential.

Shifting reef restoration focus from coral survivorship to biodiversity using Reef Carpets

To enhance the practice of farmed-coral transplantation, we conducted a trial of an approach called “Reef Carpets” (RC), which draws inspiration from the commercial turf-grass sod in land-based lawn gardening. Three 8.4m2 RCs were established on a sandy seabed, containing preselected combinations of branching corals (Acropora cf. variabilis, Pocillopora damicornis, Stylophora pistillata) with nursery recruited dwellers, and were monitored for 17-months. Corals within RCs grew, supported coral recruitment and offered ecological habitats for coral-associated organisms. While the unstable sediment underneath the RCs increased corals’ partial mortalities, corals managed to grow and propagate. The extent of fish and gastropods corallivory varied among the coral species and planulation of Stylophora transplants was significantly higher than same-size natal-colonies. The RCs provided conducive environments for fish/invertebrate communities (183 taxa), and each coral species influenced specifically species-diversity and reef-associated communities. Even dead corals played crucial roles as habitats for reef biota, sustaining >80% of the RCs diversity; hence, they should not be considered automatically as indicators of failure. RCs scaled-up reef restoration and generated, in short periods, new reefs in denuded zones with enhanced biodiversity. Yet, RCs employment on soft-beds could be improved by using more structured artificial frameworks, requiring further research efforts.

Carbonate budgets induced by coral restoration of a Great Barrier Reef site following cyclone damage

Coral carbonate production is fundamental to reef accretion and, consequently, the preservation of essential reef ecosystem services, such as wave attenuation and sustained reef biodiversity. However, the unprecedented loss of coral reefs from anthropogenic impacts has put these valuable ecosystem services at risk. To counteract this loss, active rehabilitation of degraded reef sites has accelerated globally. A variety of restoration practices exist, tailored to local site needs and reef types. For sites where there is a significant unconsolidated substrate, Mars Assisted Reef Restoration System (MARRS, or “Reef Stars”) has been utilised to contribute toward rubble stabilisation and reef accretion. However, the effect of the Reef Stars on the local carbonate budgets and structural complexity has not been assessed. For that purpose, we assess coral cover and reef complexity through a census-based approach to identify the contribution of carbonate producers and eroders alongside studying coral skeletal properties to estimate current carbonate budgets on a rehabilitated site compared to natural unrehabilitated reef and rubble patches on the mid-Great Barrier Reef. Our research identified positive ecological processes and ecological functions such as increased carbonate budget, coral cover and structural complexity at the restored site compared to the non-intervened reef and rubble patches. In general, no impacts on skeletal rigour relative to this active reef restoration were found for two key coral species and the Acropora rubble for most of the skeletal traits. However, Pocillopora damicornis hardness seemed to decrease on the restored site compared to the other sites, demonstrating different performances of coral species during restoration activities that should be considered to maximise return-on-effort of restoration activities. Overall, our data demonstrate that consideration of carbonate budgets is important for measuring success of coral restoration initiatives and that coral restoration can be a relevant tool to recover lost local carbonate budgets.