This study assessed in-cabin concentrations of diesel-associated air pollutants in six school buses with diesel engines during a typical route in suburban Austin, Texas. Air exchange rates measured by SF 6 decay were 2.60–4.55 h −1. In-cabin concentrations of all pollutants measured exhibited substantial variability across the range of tests even between buses of similar age, mileage, and engine type. In-cabin NO x concentrations ranged from 44.7 to 148 ppb and were 1.3–10 times higher than roadway NO x concentrations. Mean in-cabin PM 2.5 concentrations were 7–20 μg m −3 and were generally lower than roadway levels. In-cabin concentrations exhibited higher variability during cruising mode than frequent stops. Mean in-cabin ultrafine PM number concentrations were 6100–32,000 particles cm −3 and were generally lower than roadway levels. Comparison of median concentrations indicated that in-cabin ultrafine PM number concentrations were higher than or approximately the same as the roadway concentrations, which implied that, by excluding the bias caused by local traffic, ultrafine PM levels were higher in the bus cabin than outside of the bus. Cabin pollutant concentrations on three buses were measured prior to and following the phased installation of a Donaldson Spiracle Crankcase Filtration System and a Diesel Oxidation Catalyst. Following installation of the Spiracle, the Diesel Oxidation Catalyst provided negligible or small additional reductions of in-cabin pollutant levels. In-cabin concentration decreases with the Spiracle alone ranged from 24 to 37% for NO x and 26 to 62% and 6.6 to 43% for PM 2.5 and ultrafine PM, respectively. Comparison of the ranges of PM 2.5 and ultrafine PM variations between repetitive tests suggested that retrofit installation could not always be conclusively linked to the decrease of pollutant levels in the bus cabin.