ABSTRACT Reflectance spectroscopy has been widely utilized by researchers to characterize and predict soil nitrogen (N). This study explores the potential of reflectance spectroscopy combined with machine learning to estimate soil N levels under different N-based management practices in rice-based cropping systems. A five-year field experiment (2015–2020) was conducted at Kalyani D Block Farm, Bidhan Chandra Krishi Viswavidyalaya, Nadia, West Bengal, India, testing rice-rice, rice-wheat, and rice-potato systems with N treatments: control (N₀), 100% Neem Coated Urea (NCU), 75% NCU with 25% organics, 100% Polymer Sulfur Coated Urea (PSCU), and 75% PSCU with 25% organics. Soil samples from 0 to 15 cm depth were collected post-harvest of rabi crops (wheat and potato) and summer rice for analysis, revealing the highest available N (AN) (189.6 kg ha− 1) in PSCU-treated summer rice plots, with maximum AN in wheat (290.6 kg ha− 1) and potato (431.4 kg ha− 1) found in 75% NCU + 25% organic plots. Rice yield reached a high of 5.58 t ha− 1 with PSCU treatment, while wheat and potato produced 3.87 and 16.07 t ha− 1 with NCU + vermicompost (VC), respectively. Spectral reflectance measurements were conducted in a controlled lab environment using the ASD Field Spec 4 spectroradiometer, showing significant variability in soil spectral behavior with soil organic carbon (SOC) and N levels. Pre-processing techniques, including first-order derivation (FOD), log (1/R) transformation, and continuum removal (CR), were applied to enhance spectral data quality. Machine learning models, Support Vector Machine Regression (SVMR) and Partial Least Squares Regression (PLSR) were used to estimate SOC, AN and total N (TN) from spectral data (350–2500 nm range). Results showed that SVMR consistently outperformed PLSR for all soil properties, with continuum-removed spectra achieving R2 values of 0.98, 0.97, and 0.92, and RMSEs of 0.02%, 37.1, and 948.8 kg ha− 1 for SOC, AN and TN, respectively. This study highlights the value of integrating spectral data with machine learning for rapid, accurate soil N estimation, enabling optimized fertilizer applications to enhance crop yields and reduce environmental impact.
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