Pleurotus Ostreatus Research Articles

Investigation on β-D-glucan extraction methods from Pleurotus ostreatus mycelium and its capability to adsorb aflatoxin M1 in milk

Investigation on β-D-glucan extraction methods from Pleurotus ostreatus mycelium and its capability to adsorb aflatoxin M1 in milk

Exploring the Molecular Basis of Anticancer Activity in Various Moss Species Against Colorectal Cancer Cells

Background: Mushrooms are shown to protect against the side effects of cancer. Therefore, mushrooms with proven anticancer properties and active ingredients are fascinating in the search for new cancer drugs. Objective: In this study, the effects of extracts from Hericium coralloides (M1), Lactarius deliciosus (M2), Lepista nuda (M3), Pleurotus ostreatus (M4), and Suillus collitinus (M5) together on HCT116 were investigated. Mesenchymal stem cells (MSCs) were used to study the effect on healthy cells. Methods: MTT was used to determine cell viability. Dose-response curves were generated, the IC50 values of the compounds were calculated, and the effect of the extracts was compared using it. The FTIR was used to analyze the quantitative changes of the cellular components. Results and Discussion: The evaluation of the IC50 values of all fungal species showed that they reduced the cell viability of HCT116 cells. In contrast, no significant reduction in cell viability was observed in MSCs. Changes in the ratio of cell membrane lipids, proteins, and cell nucleic acids between control and fungal-treated HCT116 cells were detected by FTIR. Many of the chemotherapeutic agents are of plant origin, and many resources should still be explored to inhibit the side effects of cancer therapy. Conclusion: The data obtained through this experiment will serve as a reference for studies to be a new source of anticancer drugs in modern pharmacology.

Effects of hap2 deletion on mnp/vp transcription in Pleurotus ostreatus grown on lignocellulosic substrates.

The regulatory mechanisms governing expression of genes encoding lignin-modifying enzymes (LME) in white-rot fungi remain largely unexplored. Although molecular cloning has identified CCAAT-boxes frequently located 5'-upstream of these genes, their role in transcriptional regulation is not well understood. This study examines the function of hap2, a gene encoding a hypothetical protein homologous to a component of the CCAAT-binding Hap complex, in the white-rot fungus Pleurotus ostreatus. Deletion of hap2 resulted in significantly reduced Mn2+-dependent peroxidase activity and lignin-degrading capacity compared to the parental strain 20b grown on beech wood sawdust (BWS) medium. Real-time PCR revealed that vp2 transcript levels were significantly lower in hap2 deletants than in 20b grown when cultured on the three solid media consisting of BWS, holocellulose, or Avicel, but not on yeast-malt-glucose (YMG) agar plates. Additionally, glutathione S-transferase (GST) pulldown and electrophoretic mobility shift assays demonstrated that recombinant P. ostreatus Hap2, Hap3, and Hap5 expressed in Escherichia coli form a complex capable of binding to the CCAAT sequence 5'-upstream of vp2 in vitro. These results suggest that Hap2, as part of the CCAAT-binding complex, is essential for transcriptional upregulation of vp2 in P. ostreatus growing on lignocellulosic substrates. KEY POINTS: • P. ostreatus hap2 deletants were generated. • Lignin-degrading capacity was significantly reduced in the hap2 deletants. • vp2 was significantly downregulated upon hap2 deletion.

Exploring time-killing and biofilm inhibition potential of bioactive proteins extracted from two varieties of Pleurotus ostreatus

IntroductionDental caries, caused by oral microbial pathogens, are a global health concern, further exacerbated by the presence of methicillin-resistant Staphylococcus aureus (MRSA). Bioactive proteins and peptides (BAPs) exhibit potent antimicrobial properties, targeting multiple cellular mechanisms within pathogens, reducing the likelihood of resistance development. Given the antimicrobial potential of BAPs, this study aimed to compare the efficacy of BAPs extracted from cultivated (Pleurotus ostreatus, PoC) and wild (Pleurotus ostreatus, PoW) mushrooms against pathogens responsible for dental caries.MethodsBAPs were extracted from both PoC and PoW using a TCA-acetone method. Antimicrobial activities were tested against seven bacteria and one fungus using agar well diffusion and MIC determination. Antibiofilm activity was assessed via modified CV assay, while DPPH and erythrocyte lysis tests evaluated free radical scavenging.ResultsPoC showed superior antimicrobial efficacy, with lower MIC and MBC values, and disrupted biofilm integrity at increasing concentrations. PoW exhibited better antioxidant activity with higher DPPH scavenging, though its antimicrobial efficacy was slightly lower than PoC.DiscussionBoth PoC and PoW BAPs inhibited dental pathogens, with PoC showing stronger inhibition against MRSA and nystatin-resistant Candida albicans. This suggests BAPs may target additional cellular mechanisms beyond membranes, PBPs, and ergosterols. Despite PoW’s stronger antioxidant properties, both BAPs had comparable antibiofilm activity. These findings suggest complementary actions of BAPs from PoC and PoW both, in treating dental caries, offering broad-spectrum antimicrobial and antioxidant benefits.

Family Identification and Functional Study of Copper Transporter Genes in Pleurotus ostreatus

The copper transport (COPT/Ctr) family plays an important role in maintaining metal homeostasis in organisms, and many species rely on Ctrs to achieve transmembrane transport via copper (Cu) uptake. At present, the Ctr family is widely studied in plants. However, there are few reports on the use of Ctrs in edible mushrooms. In this study, the Pleurotus ostreatus CCMSSC00389 strain was used as the research object, and the addition of exogenous copper ions (Cu2+) increased the temperature tolerance of mycelia, maintained the integrity of cell membranes, and increased mycelial density. In addition, four PoCtr genes were further identified and subjected to bioinformatics analysis. Further research revealed that there were differences in the expression patterns of the PoCtr genes under different temperature stresses. In addition, the biological function of PoCtr4 was further explored by constructing transformed strains. The results showed that OE-PoCtr4 enhanced the tolerance of mycelia to heat stress and H2O2. After applying heat stress (40 °C), OE-PoCtr4 promoted the recovery of mycelia. Under mild stress (32 °C), OE-PoCtr4 promoted mycelial growth, maintained cell membrane integrity, and reduced the degree of cell membrane damage caused by heat stress. It is speculated that OE-PoCtr4 may maintain the integrity of the cell membrane and enhance the heat resistance of mycelia by regulating the homeostasis of Cu2+.

PEMANFAATAN SERBUK GERGAJI PADA PERT(UMBUHAN DAN PRODUKTIFITAS JAMUR TIRAM PUTIH (Pleurotus ostreatus) DI DESA TU’INDRAO KECAMATAN AMANDRAYA KABUPATEN NIAS SELATAN

The research objectives are know how to make white oyster mushroom white by using powder wood saw utilizing powder chainsaw at seedlings plants oyster mushrooms, to determine growth and productivity oyster mushroom white Research method this is by method descriptive with a qualitative approach, describe, analyze phenomena on the object of research. This type of research is powder utilization chainswa wood to growth and productivity mushroom oysters white in Tu'indrao village, Amandraya findings research i.e, making media mushroom by preparing 100 kg powder and mixed with other ingredients, utilitazion powder chainsaw with the inoculation stage f2 mushroom spawn number ±1-2 spatula, growth and productivity mushroom oysters 1-2 days after inoculation to grow fruit, 3-5 days harvest mushroom, yield 5 kg per 1 harvest and ready to be utilized by the community as a vegetable. Research from conclusion this is utilizing media powder chainsaw wood for mold growth oysters is, before the media is mixed it should be powder chainsaw composted and sterilized especially first to makes the texture softer my suggestions in research this is, this research is expected to be an alternative to develop other research that related with cultivation mushroom oysters white (Pleurotus ostreatus) by using powder chainsaw wood. For students, as source knowledge in carrying out learning activities at eyes taxonomy lecture plants level low in program study education biology.

Treatment of Cigarette Butts: Biodegradation of Cellulose Acetate by Rot Fungi and Bacteria

This study demonstrated the biodegradation of two different brands of cigarette butts (CBs), which are primarily composed of cellulose acetate, by four distinct microorganisms. These included the white rot fungus Pleurotus ostreatus, the brown rot fungus Lentinus lepideus, and the bacteria Bacillus cereus and Pseudomonas putida. After 31 days of treatment, weight loss measurements revealed a mass loss of 24–34%, where B. cereus exhibited the greatest efficacy in terms of mass loss for both brands of CBs. Fourier-Transform Infrared Spectroscopy (FTIR), confocal microscopy, and scanning electron microscopy (SEM) confirmed changes in the surface of the CBs, attributable to structural wear and material breakdown, indicating effective biodegradation by the evaluated microorganisms. Furthermore, the analyses confirmed changes in the surface of the CBs, attributable to structural wear and material breakdown, indicating effective biodegradation by the evaluated microorganisms.

The biosynthesis and impacts of cytokinins on growth of the oyster mushroom, Pleurotus ostreatus

ABSTRACT While a lot is known about cytokinins (CKs) and their actions at the molecular and cellular levels in plants, much less is known about the function of CKs in other kingdoms such as fungi. CKs have been detected in a wide range of fungal species where they play roles ranging from enhancing the virulence of phytopathogens to fortifying plant growth when secreted from fungal symbionts. However, the role of CKs where they concern fungal physiology, apart from plant associations, remains largely uncharacterized. Profiling by UHPLC-HRMS/MS (ultrahigh-performance liquid chromatography–high-resolution tandem mass spectrometry) revealed that Pleurotus ostreatus (oyster mushroom) produces CKs in vitro in both liquid and solid cultures. During fungal growth, CK profiling patterns were consistent with previous suggestions that tRNA degradation products might play a role in the physiological development of fungi. It confirms that those products are CKs that act as fungal growth regulators. Moreover, P. ostreatus was shown to respond to exogenous applications of aromatic and isoprenoid CKs, and their effects were dependent on the dose and CK type in a biphasic manner consistent with hormone action. N 6-benzyladenine (BAP), kinetin (KIN), N 6-isopentenyladenine (iP), and trans-zeatin (tZ) bioassays all revealed hormesis-type responses. Accordingly, at low doses, mycelium colony diameter, biomass accumulation, and changes in morphology were stimulated, whereas at high doses only inhibitory effects were observed. Thus, CKs may act as “mycohormones” and consequently have potential for applications in fungal agriculture and medicinal compound production.

Deep Learning-Based Classification of Macrofungi: Comparative Analysis of Advanced Models for Accurate Fungi Identification

This study focuses on the classification of six different macrofungi species using advanced deep learning techniques. Fungi species, such as Amanita pantherina, Boletus edulis, Cantharellus cibarius, Lactarius deliciosus, Pleurotus ostreatus and Tricholoma terreum were chosen based on their ecological importance and distinct morphological characteristics. The research employed 5 different machine learning techniques and 12 deep learning models, including DenseNet121, MobileNetV2, ConvNeXt, EfficientNet, and swin transformers, to evaluate their performance in identifying fungi from images. The DenseNet121 model demonstrated the highest accuracy (92%) and AUC score (95%), making it the most effective in distinguishing between species. The study also revealed that transformer-based models, particularly the swin transformer, were less effective, suggesting room for improvement in their application to this task. Further advancements in macrofungi classification could be achieved by expanding datasets, incorporating additional data types such as biochemical, electron microscopy, and RNA/DNA sequences, and using ensemble methods to enhance model performance. The findings contribute valuable insights into both the use of deep learning for biodiversity research and the ecological conservation of macrofungi species.

Physical and Mechanical Properties of Indian Oyster Mushroom Mycelium/Sawdust Composites for Biodegradable Packaging Materials

Mycelium-based composite (MBC) offers an excellent sustainable alternative to hydrocarbon-based materials, especially styrofoam for packaging, due to its abundance of fungal mycelium that grows quickly on agricultural substrates, its biodegradable and its lightweight. The mycelium of a commercial mushroom species, Pleurotus ostreatus (PO), is used to fabricate MBC for packaging materials. Another species, Pleurotus pulmonarius (PP), prefers warmer weather, making it more common in tropical countries. Nevertheless, there is a lack of studies of PP mycelium-based composites and their mechanical and physical properties. This study investigated the physical and mechanical properties of PP mycelium/sawdust composite and compared to PO mycelium/sawdust composite. The results showed that the average density of PP/sawdust and PO/sawdust composites were 292.14 and 272.17 kg/m3, respectively, which fell within the range of low-density polyurethane foam. The final mass gain due to water absorption into PO/sawdust specimens was 144.04%, 1.41 times lower than PP/sawdust specimens. Furthermore, PP/sawdust composite exhibited 7.5 times faster water absorption rate than PO/sawdust composite, indicating that PO/sawdust had better water resistance. The PP/sawdust composite produced an equivalent compressive modulus to the PO/sawdust composite under compression up to 1.34 MPa of maximum value. Thus, the PP/sawdust composite showed excellent potential for substitution of biodegradable packages made from PO/sawdust composite as they contributed the equivalent strength; however, the PO/sawdust composite exhibited superior water resistance to the PP/sawdust composite. Consequently, PO/sawdust should be more advantageous if the biodegradable packaging is required to be of strength as high as the low-density polyurethane foam and of compatible water resistance.

Evaluation of the textile dye removal process using the residual substrate from Pleurotus ostreatus mushroom production

O lançamento de efluentes têxteis sem tratamento, ou submetidos a um tratamento ineficaz, ocasiona graves problemas nos ecossistemas aquáticos. Neste contexto, o objetivo deste estudo foi avaliar a capacidade e o processo de remoção do corante têxtil Drimaren Red CL-7B, empregando o substrato residual da produção do cogumelo Pleurotus ostreatus. Para tanto, primeiramente elaborado um planejamento experimental 3², considerando as variáveis pH e concentração de substrato, buscando-se avaliar a remoção de corante proveniente de efluente têxtil sintético, durante seis horas de tempo de contato. As concentrações do substrato aplicado ao efluente foram 50, 100 e 150 g L-1 e os valores de pH empregados foram 5, 7 e 9. Após a determinação da melhor condição experimental dentre as avaliadas, também foram estudados os possíveis mecanismos de remoção ocorridos e a atividade enzimática do micélio presente no substrato. Verificou-se que a melhor condição operacional foi obtida com pH 5 e concentração de 150 g L-1. Sob essas condições, a eficiência de remoção de corante alcançada foi próxima de 70%. Observou-se que o principal mecanismo de remoção foi a adsorção e um tempo de contato de 30 minutos já permite obter resultados satisfatórios. Além disso, os resultados da atividade da lacase demonstraram que sua eficiência é elevada em condições de pH ácido, e que esta fica inativa em condições de pH alcalino. Concluiu-se que o substrato residual apresenta elevado potencial para o tratamento de efluentes têxteis em decorrência da ação simultânea das enzimas lignolíticas e da adsorção na remoção de corantes, bem como, pela possibilidade de valorização de um resíduo agroindustrial.

Exogenous Salicylic Acid Regulates Fruiting Body Development, Secondary Metabolite Accumulation, Cell Wall Integrity, and Endogenous Salicylic Acid Content under Heat Stress in Pleurotus ostreatus.

High-temperature or heat stress (HS) represents a significant environmental challenge that adversely affects crop growth and poses a substantial threat to agricultural production. Pleurotus ostreatus, recognized as the second most widely cultivated edible fungus worldwide, is particularly susceptible to the detrimental effects of HS. Enhancing the HS resistance of P. ostreatus is crucial for increasing its yield. In a prior investigation, we discovered that salicylic acid (SA) enhanced the resistance of P. ostreatus mycelia to HS through a metabolic rearrangement. The present study further investigated the effects of SA on P. ostreatus under HS. Cultivation experiments revealed that exogenous SA improved the mycelium recovery growth rate, yield, and fruiting body quality after HS. Further experiments revealed that exogenous SA mitigated the damage to the MAPK-Slt2 signal produced by HS while maintaining cell wall integrity. Furthermore, we hypothesized that the phenylalanine ammonia-lyase pathway might serve as a source for SA. In this context, we identified two salicylic hydroxylases, Po1102164 and Po1104438. Both HS and exogenous SA were found to elevate intracellular SA levels, thereby enhancing the resistance of P. ostreatus to HS.

Influence of environmental conditions on the growth of Pleurotus ostreatus in sand

Pleurotus ostreatus, a saprotrophic fungus, has been proposed for the remediation of organic contaminants in soils and more recently for modifying the hydraulic and mechanical behaviour of granular soils. The in situ performance of fungal-based biotechnologies will be controlled by the fungal growth and associated biochemical activity that can be achieved in soil. In this study, the influence of environmental conditions (temperature, degree of saturation), substrate type (lignocellulose and spent coffee grounds) and concentration on the mycelium growth of P. ostreatus in sand are investigated. Furthermore, the evolution of growth/survival indicators (respiration, ergosterol concentration) and enzymatic activity (laccase, manganese peroxidase) are investigated. Temperature was shown to have a strong influence on the growth of P.ostreatus in sand: growth was observed to be delayed at low temperatures (e.g. 5 °C), whereas growth was prevented at high temperatures (e.g. 35 °C). No growth was observed at very low degrees of saturation (Sr=0% and 1.2%), indicating there is a critical water content required to support P.ostreatus growth. Within the mid-range of water contents tested radially, growth of P.ostreatus was similar. However, growth under saturated soil conditions was restricted to the air-water atmosphere due to the requirement for oxygen availability. Low substrate concentrations (1%–5%) resulted in high radial growth of P.ostreatus, whereas increasing substrate content further acted to reduce radial growth, but visual observations indicated that fungal biomass density increased. These results are important for understanding the feasibility of P.ostreatus growth under specific site conditions and for the design of successful treatment strategies.

Transcription factor Pofst3 regulates Pleurotus ostreatus development by targeting multiple biological pathways

Pleurotus ostreatus is a popular edible mushroom cultivated worldwide. However, the mechanism of P. ostreatus primordia formation is unclear. Pofst3 is a MHR superfamily transcription factor, which has the function of regulating primordia formation. In this study, the target genes of Pofst3 in P. ostreatus were identified by DAP-Seq approach at the genome level, 1481 peaks were obtained and the Pofst3 binding motif sequence was GARGRVGARGAR. The interaction between transcription factor Pofst3 and this motif was confirmed in vitro and in vivo through electrophoretic mobility shift (EMSA) and yeast one-hybrid screening (Y1H) assays. Among the top 20 GO enrichment results, most were related to transcriptional regulation, and some transcription factor encoding genes, such as HMG-box (gene_5346), MADS-box (gene_86), FOG (gene_6211) and RFX (gene_3496) were obtained. Besides basic metabolism, MAPK signaling pathway, Inositol phosphate metabolism, Glycosylphosphatidylinositol (GPI)-anchor biosynthesis and Pentose phosphate pathway were significantly enriched in the KEGG pathway analysis. The expression levels of randomly selected 11 genes, some transcription factor genes, and genes involved in metabolic pathways in wild and Pofst3 transgenic P. ostreatus strains indicated that target genes likely involved in the development of the P. ostreatus primordia. These results indicated that transcription factor Pofst3 ultimately negatively regulated the development of P. ostreatus primordia very likely through regulating a series of biological pathways.

Substrate evaluation for the growth of Pleurotus ostreatus mushroom

The type of substrate is a critical factor influencing the growth and yield of oyster mushroom (Pleurotus ostreatus). Seven substrates, namely wheat straw, paddy straw, maize straw, jowar straw, soybean straw, green gram straw, and black gram straw, were evaluated for their effect on oyster mushroom productivity. Among these, the highest yield was observed on soybean straw (815.66 g/kg straw) with a biological efficiency (B. E.) of 81.56 %, followed by yield on wheat straw (721.33 g/kg straw) with 72.13 % B. E. The lowest yield was recorded on maize straw (605.66 g/kg straw) with a B. E. of 60.56 %.

The Economics of Production and Marketing of Oyster Mushroom (Pleurotus ostreatus) in Bukidnon, Philippines

Bukidnon, a province in Region X, is known for its high percentage of oyster mushrooms, accounting for 85.15 percent of total produce. This research aimed to determine production and marketing practices, yield per fruiting bag, marketing channels, and costs. We observed three marketing channels, and the average yield per bag was 0.18 grams per day. Variables affecting productivity included extension visits, educational attainment, culture house temperature, and substrate types. However, producers faced issues like spawn unavailability, poor development, and spoilage. The lack of government equipment support and lower prices led to many farmers stopping production, especially during the pandemic.

Pleurotus ostreatus is a potential biological control agent of root-knot nematodes in eggplant (Solanum melongena)

IntroductionThe management of root-knot nematodes has predominantly been based on use of chemicals, which are detrimental to the environment and human health. Biological control provides alternative management. This study evaluated the potential of using Pleurotus ostreatus, an edible mushroom species to control Meloidogyne spp. in eggplants.MethodsIn vitro, the mortality of juveniles (J2) of Meloidogyne spp. were assessed i) P. ostreatus - water suspension with actively growing mycelia, and ii) different dilutions of P. ostreatus PDB broth culture filtrates. In the screen house nematicidal potential of P. ostreatus was tested on eggplants using artificially inoculated soils in a screen house. To attain this, juveniles (J2) of Meloidogyne spp. were inoculated at the base of plants in pots containing P. ostreatus colonized millet grains mixed in 3 kg of soil. The galling index (GI) (scale of 0 to 5), root growth and nematode populations in the different treatments were assessed.Results and discussionMortality of nematodes in the P. ostreatus - water suspension significantly increased with time, reaching over 88% at 48 h and 95% at 72 h. Mortality in undiluted filtrate was consistently significantly higher than the diluted filtrates and control without P. ostreatus. Mortality in the undiluted filtrate increased to 95% at 48 h. When 50 g of P. ostreatus-millet culture was mixed with 3 kg autoclaved pot soil, a GI of 0.95 was observed, dropping to 0.70 when the inoculum was doubled to 100 g. For the treatment without P. ostreatus, a high GI of 2.4 was scored. A significant difference in eggplant root growth and nematode population at (p = 0.02) was found across the treatments. The findings from this study for both in-vitro and pot assay suggest that P. ostreatus and its substrate are potential biological control agents for plant parasitic nematodes in eggplants.

Duration of Light Exposure Using White Light-Emitting Diode (LED) on the Performance of White Oyster Mushroom (Pleurotus ostreatus L.)

Background: In the dynamic world of mushroom cultivation, optimizing growth conditions is paramount for maximizing yield and quality. This research sought to evaluate the influence of varying hours of exposure to white light through light-emitting diodes (LED) on growth and yield performance of white oyster mushroom (Pleurotus ostreatus L.). Its aim was to determine the most effective light exposure duration for enhancing mushroom growth and yield. Research Design: Using a completely randomized design (CRD), the study used four treatments: 8 hours of lighting (T1), 12 hours of lighting (T2), 16 hours of lighting (T3), and a control group that received natural light (T4). The study was conducted in Palayamanan (8° 27’ 5.3118" N and 126° 9’ 59.7739" E) area at North Eastern Mindanao State University’s -Tagbina Campus from July 2023 to November 2023. Results: Results from the study provided interesting insights into the indirect nature of the relationship between light exposure and mushroom development. The findings of the study indicate that the duration of light exposure significantly affects the growth and yield of white oyster mushrooms. Both mycelium growth (29.80 days) and fruiting body formation (7.83 days), as well as harvesting time (10.87 days), were improved by 12 hours of lighting, while the control group consistently showed poor performance. Conclusion: Based from the analysis, it is evident that 12 hours of lighting consistently performed very well, with the exception of a minor under-performance in the case of yield per harvest, suggesting that 12 hours might be the ideal duration. Hence, 16 hours of lighting also demonstrated some advantages in certain variables, indicating potential for further study.

A NOVEL GOLD NANOPARTICLE‐IONIC LIQUID NANOSTRUCTURE MODIFIED Pleurotus Ostreatus BASED MICROBIAL BIOSENSOR SYSTEM FOR BISPHENOL A

The current study develops the first and novel gold nanoparticle‐ionic liquid nanostructure modified microbial biosensor based on <i>Pleurotus ostreatus</i> for the sensitive determination of Bisphenol A <b>. </b>In the construction of the microbial biosensor, lyophilized <i>P.ostreatus</i> cells were modified with ionic liquid‐cysteamine mixture on a gold electrode which was modified with 1,6‐hexanedithiol and gold nanoparticle. The measurement principle of the biosensor is based on the detection of changes in current in a phosphate buffer system (pH 7.5, 50 mM and containing 5.0 mM K <sub>3</sub>[Fe(CN) <sub>6</sub>]) in the potential range of ‐0.2 to +0.6 V depending o

Effects of spent substrate of oyster mushroom (Pleurotus ostreatus) on ruminal fermentation, microbial community and growth performance in Hu sheep.

The study aimed to evaluate the effects of Pleurotus Spent Mushroom Substrate (P.SMS) on the rumen microbiota, encompassing bacteria and fungi, as well as their interactions in Hu sheep. A total of forty-five 3-month-old Hu sheep were randomly assigned to five groups. Each group was fed diets in which whole-plant corn silage (WPCS) was substituted with P.SMS at varying levels: 0% (CON), 5% (PSMS5), 10% (PSMS10), 15% (PSMS15), or 20% (PSMS20). The results indicated that higher proportions of P.SMS during the experimental period might have a detrimental effect on feed utilization efficiency, kidney function, and blood oxygen-carrying capacity. Notably, moderate levels of P.SMS, specifically below 15%, were associated with improvements in rumen NH3-N levels and absorption capacity. The results indicated that (1) PSMS20 exhibited a significantly higher feed-to-gain ratio compared to CON (P < 0.05); (2) PSMS15 showed a significantly higher NH3-N content than CON, PSMS5, and PSMS20. Additionally, PSMS10 and PSMS20 had elevated concentrations of NH3-N compared to CON and PSMS5 (P < 0.05); (3) The length and width of rumen papillae were significantly greater in PSMS20 compared to CON and PSMS5 (P < 0.05); (4) Creatinine levels were significantly higher in PSMS20 than in CON, PSMS5, and PSMS10 (P < 0.05); (5) By the conclusion of the experiment, hemoglobin concentration in PSMS20 showed a significant increase compared to CON (P < 0.05). Furthermore, the addition of P.SMS influenced microorganisms at both the phylum and genus levels: (1) At the phylum level, the prevalence of Patescibacteria was significantly lower in PSMS20 compared to the other groups; (2) PSMS15 exhibited significantly higher relative abundances of Basidiomycota compared to CON and PSMS10, while PSMS20 also demonstrated significantly higher relative abundances compared to CON (P < 0.05); (3) At the genus level, the prevalence of Candidatus_Saccharimonas in PSMS20 was significantly lower than in PSMS5, PSMS10, and PSMS15. Conversely, the prevalence of Phanerochaete in PSMS15 was notably higher than in CON and PSMS10, and it was also significantly elevated in PSMS20 compared to CON (P < 0.05); (4) Correlation analysis indicated no significant correlation between changes in the structure of bacterial and fungal communities. Considering these findings, a high percentage of P.SMS negatively impacted feed utilization efficiency, blood oxygen carrying capacity, and kidney function, while a moderate percentage of P.SMS promotes rumen absorption capacity, indicating that feeding 10% P.SMS is optimal.