The panikoorka plant is a noble plant which is packed with various benefits, belonging to the family Lamiaceae as it may help in lowering blood sugar levels, maintaining heart health, and helps in digestive system, the stem of panikoorka is also having the anti-cancer activity some studies also prove that the extract of panikoorka stem are effective against the acne causing bacteria (anti-microbial).We are formulating the anti-fungal cream with the extract of panikoorka stem, we have collected the different extracts of the panikoorka stem, we have formulated 2 different formulations with the stem of panikoorka (Plectranthus barbatus).The Plant extract is used to treat different types of infectious disease, hence microbial screening attempted to prove its anti-bacterial and anti-fungal activity. The demonstration of therapeutic efficacy was achieved through the use of defined settings to restrict microbial development.The paper disc diffusion method was employed in this work to assess the synthetic compounds' in vitro antifungal efficacy. One technique for figuring out the relative efficacy of the anti-fungal activity is the paper disc diffusion method. The anti-microbial drugs' toxicity and their capacity to permeate into the medium both affect the outcomes of this approach. Although it is not as effective as standard, our produced cream has antifungal action against both Aspergillus niger and Candida albicans bacteria.