Background: Osteoarthritis (OA) is condition characterised by progressive degeneration of joint cartilage, eventually leading to deformity of the joint. OA causes pain and disability and impacts on quality-of-life. Intra-articular platelet rich plasma gives favourable outcomes in OA knee; however, efficacy may be affected by method of preparation. Hence, this study comparing single spin and double spin platelet-rich plasma (PRP).Methods: A randomised controlled trial done among patients of OA who visited Department of Physical Medicine and Rehabilitation, Regional Institute of Medical Sciences, Imphal during August 2019-July 2021. Patients with OA knee having radiological findings of Kellgren Lawrence (KL) grade 2 and 3 (n=62) randomized into single spin PRP, (n=31) and double spin PRP, (n=31) groups and intra-articular PRP injection was given.Results: Baseline characteristics were not statistically significant. At the end of 1 week, improvement in mean difference of VAS was observe however not statistically significant (p=0.71). At end of 12 weeks and 24 weeks, there were statistically significant improvement in both mean difference of VAS (p=0.00) and WOMAC (p=0.002). Post intervention, out of the single spin PRP group, (64.51%, n=20) had complaints of adverse effects swelling, pain, redness whereas (6.45%, n=2) patients who received double spin PRP had adverse effects.Conclusions: Platelet rich plasma obtained by double spin method has long term improvement in both pain and function in patients with KL grade 2 and 3 OA knee than PRP from single spin method. Patient who received single spin PRP has more frequent post injection flare ups.