Platelet-rich Plasma In Healing Research Articles

Comparison of efficacy of topical platelet-rich plasma in healing of split thickness skin grafting versus conventional split thickness skin grafting

Comparison of efficacy of topical platelet-rich plasma in healing of split thickness skin grafting versus conventional split thickness skin grafting

Role of Platelet Rich Plasma in Healing of Chronic Venous Leg Ulcer: A Randomized Controlled Clinical Trial

Role of Platelet Rich Plasma in Healing of Chronic Venous Leg Ulcer: A Randomized Controlled Clinical Trial

Role of Platelet Rich Plasma in Healing of Chronic Leg Ulcers

Platelet-rich plasma (PRP) is characterized as a segment of the plasma part of autologous blood having a platelet fixation above standard. PRP likewise has been alluded to as platelet-improved plasma, platelet-rich concentrate, autologous platelet gel, and platelet releasate. To research the advantages of infusing platelet rich plasma in mending of constant leg ulcers. This is a solitary – focus, planned, randomized, controlled examination that was directed in General Surgery Department, Faculty of Medicine, Benha University incorporating 100 patients giving persistent leg ulcers and introduced to us between January 2018 to June 2018. This investigation was completed in 100 patients with ongoing leg ulcers were remembered for this examination, patients were arbitrarily partitioned in two equivalent gatherings An and B. The magnificent outcomes appeared in ongoing investigations have just served to affirm the great results depicted in our examination. In ten patients that we treated, the outcomes got following consummation of the uses of PRP were acceptable or phenomenal. The utilization of PRP in remedial cycles identified with Plastic, Reconstructive and Esthetic Surgery is presently a genuine alternative in generally open and private emergency clinics in numerous nations; circumstance that shows the advances that have happened over ongoing years in tissue recovery treatment.

Histological Study of the Effect of Platelet Rich Plasma on Experimentally Induced Skeletal Muscles Injury in Adult Male Albino Rats

Background: Muscle injuries are common and may be associated with impaired functional capacity, especially among athletes. Ordinary lines of treatment result in improper healing of the damaged tissue with prolonged time for recovery, so it was essential to try new lines of treatment for these injuries to get advantageous effect on the healing process. One of these new treatments is platelet-rich plasma (PRP), which is used to improve healing of many lesions such as skin and sports injury. Aim of the work: This work was carried out to evaluate the role of platelet rich plasma in healing of experimentally induced skeletal muscle injury in adult male albino rat. Materials and methods: Thirty adult male albino rats were divided Into: Group I: donor group to obtain PRP from their peripheral blood and muscle specimens from their gasterocnemius muscle. Group II: was exposed to bilateral gastrocnemius muscles injury and was left without treatment. Group III: Bilateral gastrocnemius muscles were injured and immediately treated locally with PRP intramuscularly. Muscle specimens were excised after 1, 7and 21 days from the onset of injury and were processed for light and electron microscopic examination. Results: PRP treated group demonstrated early formation of many myotubes starting from the 7th day after injury onwards in association with ultrastructural features of activated satellite cells together with restoration of normal histological features of muscle fibers. Conclusion: local injection of PRP into the injured gastrocnemius muscle resulted in early activation, proliferation and differentiation of satellite cells resulting in earlier and enhanced myogenesis.

The role of autologous platelet-rich plasma in healing of gaping and chronic wounds

Objective The aim was to evaluate the efficacy of platelet-rich plasma (PRP) in the treatment of gaping and chronic wounds. Background Nonhealing ulcers are a major health problem with high cost in terms of human and material resources. Application of autologous PRP has been a major breakthrough for the treatment of nonhealing ulcers, as it is an easy and cost-effective method and provides the necessary growth factors that enhance wound healing. Patients and methods A study was performed on 28 patients with nonhealing ulcers of various etiologies. Overall, 19 patients were treated with PRP at weekly intervals for a maximum of seven treatments and nine patients were treated with saline gauze dressings on alternative days. At the end of the 7, 14, 21, and 28-day periods, reduction in size of the ulcers (area and volume) was assessed and followed up till the healing completed, and the patients were followed up for a period of 12 weeks after treatment. Results The mean age ± SD of the patients was 39.68 ± 12.11 years. Of 19 ulcers in study group, there were two venous ulcers, nine traumatic ulcers, five pressure ulcers, and three diabetic ulcers. The mean ± SD duration of healing of the ulcers was 49.84 ± 22.23 days in study group and 108.67 ± 5.64 days in control group (P Conclusion Conventional therapies do not provide satisfactory healing for chronic nonhealing ulcers as they are not able to provide the necessary growth factors essential for the healing process. PRP is a safe, affordable, biocompatible, and simple office-based procedure for the treatment of nonhealing ulcers.

The role of fish scale derived scaffold and platelet rich plasma in healing of rabbit tibial defect: an experimental study

Fish scale is rich in collagen type I and hydroxyapatite, resembling bone structure. It is readily available, cost effective and can compensate for the limitations of grafting methods such as unavailability, zoonotic disease transmission, and high cost. The aim of this study was to evaluate in vivo the fish scale potential and the possible synergistic effect of platelet rich plasma (PRP) with this scaffold in bone regeneration. Fifteen male white New Zealand rabbits were randomly divided into six groups, each involving 5 limbs. Full thickness bicortical defects were created in the proximal tibia of both pelvic limbs of rabbits. The defect was left untreated in the negative control group. In experimental groups the defect was filled with PRP (group 1), cellular fish scale (group 2), combination of cellular fish scale and PRP (group 3), acellular fish scale (group 4), and a combination of acellular fish scale and PRP (group 5). Fresh fish scales were decellularized to increase biocompatibility and reduce immunity reactions. Decellularization was confirmed by DAPI (4',6-diamidino-2-phenylindole) staining. The microstructure and surface characteristics of fish scales were assessed by scanning electron microscopy (SEM). Histopathological evaluation of bone healing was performed on day 56. Although there was no significant difference in the bone union among experimental groups, the union was superior in all experimental groups compared to control. Spongiosa and cortex formation were superior in the acellular groups. Furthermore, PRP promoted bone marrow formation. We concluded that fish scale is a biocompatible scaffold with a high regenerative potential.

Treatment of Partial Rotator Cuff Tear with Ultrasound-guided Platelet-rich Plasma.

Background:The treatment of symptomatic partial rotator cuff tear has presented substantial challenge to orthopaedic surgeons as it can vary from conservative to surgical repair. Researches have established the influence of platelet rich plasma in healing damaged tissue. Currently very few data are available regarding the evidence of clinical and radiological outcome of partial rotator cuff tear treated with ultrasound guided platelet rich plasma injection in English literature.Materials and Methods:20 patients with symptomatic partial rotator cuff tears were treated with ultrasound guided platelet rich plasma injection. Before and after the injection of platelet rich plasma scoring was done with visual analogue score, Constant shoulder score, and UCLA shoulder score at 8 weeks and third month. A review ultrasound was performed 8 weeks after platelet rich plasma injection to assess the rotator cuff status.Results:Our study showed statistically significant improvements in 17 patients in VAS pain score, constant shoulder score and UCLA shoulder score. No significant changes in ROM were noted when matched to the contra-lateral side (P < 0.001) at the 3 month follow-up. The study also showed good healing on radiological evaluation with ultrasonogram 8 weeks after platelet rich plasma injection.Conclusion:Ultrasound guided platelet rich plasma injection for partial rotator cuff tears is an effective procedure that leads to significant decrease in pain, improvement in shoulder functions, much cost-effective and less problematic compared to a surgical treatment.

To evaluate the role of platelet-rich plasma in healing of acute diaphyseal fractures of the femur

PurposeNew research is focusing on the use of autologous growth factors to increase the effect of bone fracture healing while decreasing the amount of healing time for the patient. Platelets have been demonstrated to be the natural storage vessel for several growth factors and cytokines that promote blood coagulation, tissue repair, and the process of bone mineralization. The present study aims to evaluate the role of platelet-rich plasma (PRP) in healing of acute femoral shaft fractures radiologically. We hypothesize that it provides artificial hematoma and releases various growth factors. MethodsThis prospective randomized study was carried out in 72 patients of traumatic fracture of the femoral shaft operated with interlocking nails (closed or open). Patients were divided into two groups: study group A (n = 33) treated with intramedullary nailing & PRP injection/gel application in the same setting; and control group B (n = 39) treated with intramedullary nailing without PRP application. Both groups were further divided into two subgroups. Study group included subgroup A1 (n = 14) operated with closed intramedullary nailing and PRP injection at the fracture site under radiological control, and subgroup A2 (n = 19) operated with open intramedullary nailing and PRP gel along with fibrin membrane application at the fracture site; while control group included subgroup B1 (n = 16) operated with closed intramedullary nailing, and subgroup B2 (n = 23) operated with open intramedullary nailing. Radiological assessment of fracture healing was done by measuring the cortex to callus ratio every month till union at 6 months. ResultsMeasurements of mean cortex to callus ratio revealed significant difference between the groups A & B at third and fourth months. Measurements of mean cortex to callus ratio did not reveal significant difference between the subgroups at first and sixth months. A statistically significant difference was observed between subgroups A1 & B2 and B1 & B2 at the second month; between subgroups A1 & B2, A2 & B2 and B1 & B2 at the third month; and between subgroups A1 & B2 at fourth and fifth months. ConclusionPRP has no effect on femoral shaft fracture healing treated with closed intramedullary nailing. However, PRP and matrix scaffold provided by fibrin membrane may provide an artificial hematoma effect in the initial phase of healing in open or failed closed intramedullary nailing.

Autogenous Platelet Rich Plasma in Healing of Bone Defects

Purpose: To evaluate the efficacy of PRP in regeneration of bone in defects of the oral and maxillofacial region.&#x0D; Methods: A comparative nonrandomized study including 40 patients with bony defects of cystic origin in the maxillofacial region was carried out in the Department of Oral and Maxillofacial Surgery. Patients were divided into two groups: group A received PRP and in group B PRP was not used. Postoperatively, the patients were monitored regularly by radiographs to evaluate new bone growth at 1st ,3rd and 6th month clinically and radiographically.&#x0D; Results: Faster bone healing in was observed in the patients in whom PRP was used at every follow up.&#x0D; Conclusion: Defects filled with PRP showed comparatively earlier and faster bone regeneration. JMS 2013;16(2):90-94

Role of platelet rich plasma in healing after mandibular third molar surgery

It is well known that platelets have many functions beyond that of haemostasis. Platelets contain important growth factors, which on secretion hastens the wound healing with rapid and dense bone regeneration. Platelet rich plasma (PRP) is an autologous concentration of human platelets in a small volume of plasma consisting of at least seven fundamental protein growth factors that promotes wound healing. This paper presents a prospective study undertaken in Department of Oral and Maxillofacial Surgery, Rama Dental College & Hospital, Kanpur which includes 20 patients (males/females) for extraction of bilateral impacted mandibular third molar on a purposive sampling who fulfill certain inclusion and exclusion criteria.