Platelet Hematocrit Research Articles

Right ventricular function indices and platelet parameters for early prediction value of bronchopulmonary dysplasia: a retrospective study

BackgroundTo examine the value of early echocardiographic indices for the right ventricular function combined with platelet(PLT) parameters for predicting bronchopulmonary dysplasia (BPD) in preterm infants.MethodsThis retrospective study included infants with gestational age (GA) below 32 weeks, who were admitted to the neonatal intensive care unit(NICU). The detection rate of tricuspid regurgitation jet velocity (TRVJ), ventricular septal flattening, pulmonary artery widening, right ventricular dilation, and right atrial enlargement on the 7th day of life (DOL 7) were compared between BPD and non-BPD infants. Echocardiographic indices of the right ventricular function including tricuspid annular plane systolic excursion (TAPSE) and right ventricular index of myocardial performance (RIMP) were measured on 1 day of life (DOL 1)、on DOL 7 and on 14 day of life (DOL 14) respectively. The PLT parameters including the PLT count, mean platelet volume (MPV), platelet hematocrit (PCT) level, and platelet distribution width (PDW) were measured on the DOL 1,DOL 7, and DOL 14. Multivariate logistic regression was used to analyze the relationship between these parameters and BPD. Receiver operating characteristic curve analysis was performed to assess the predictive value of the right ventricular function indices and PLT parameters for BPD.ResultsA total of 220 preterm infants were included in this study, and of these, 85 infants developed BPD among them. The RIMP of the BPD group on DOL 14 was higher than that of the non-BPD group (P < 0.05). The TAPSE of the BPD group on DOL 14 was lower than that of the non-BPD group (P < 0.05). The PLT count of the BPD group on DOL 1 was lower than that of the non-BPD group (P < 0.05), and the MPV of the BPD group on DOL 1 was higher than that of the non-BPD group (P < 0.05). Using multivariate logistic regression, GA、invasive mechanical ventilation duration ≥ 7 days、 PLT、 MPV、 TAPSE and RIMP were found to be independent risk factors for BPD. The area under the receiver operating characteristic curve was 0.846 (95CI: 0.794∼0.899), which improved when using right ventricular function indices combined with platelet parameters.ConclusionTAPSE and RIMP combined with PLT count and MPV can help identify preterm infants at an increased risk of developing BPD.

Comparative analysis of the clinical features of nonvalvular atrial fibrillation among Tibetan, Han, and Hui patients in Qinghai Province, China

BackgroundGlobal nonvalvular AF rises, impacting health severely. In Qinghai, China's diverse setting, studying AF among varied ethnic groups is crucial ObjectivesThe purpose of this study was to compares cardiac features in AF among Tibetan, Han, and Hui patients to develop tailored prevention and treatment strategies for this region, the goal was to enhance the understanding of AF and provide an empirical basis for developing prevention and treatment strategies specific to this region MethodsThis study included a total of 3445 Tibetan, Han, and Hui patients diagnosed with nonvalvular atrial fibrillation and treated at the Qinghai Cardiovascular and Cerebrovascular Specialist Hospital, China, between January 2019 and January 2021. We analyzed the differences in cardiac structure, comorbidities, and other influencing factors among the different ethnic groups ResultsWe found significant differences in gender, age, smoking history, lone atrial fibrillation, left heart failure, dilated cardiomyopathy, and diabetes between Tibetan, Han, and Hui patients (P < 0.05). Tibetan, Han, and Hui patients also differed with regard to left ventricular end-diastolic volume, left ventricular ejection fraction, fractional shortening, NT-proBNP, glycated hemoglobin, red blood cell distribution width, platelet count, platelet hematocrit, platelet distribution width, homocysteine (Hcy), C-reactive protein, and superoxide dismutase (SOD) (P < 0.05) ConclusionOur study revealed variations in comorbidities, cardiac structure, and blood indexes among Tibetan, Han, and Hui AF patients, highlighting distinct patterns in complications and biomarker levels across ethnic groups.

Analysis of the influencing factors in the long-term survival of esophageal cancer.

To analyze the prognosis and diagnostic value of relevant hematological indexes on the survival status of patients with esophageal squamous cell carcinoma after radical surgery. This study included 206 patients with esophageal cancer who underwent surgical R0 resection. The data, including the basic information, preoperative blood routine, albumin, fibrinogen, surgery-related information, postoperative pathology, and overall survival, of the patients were compared. The survival and death groups showed a significant difference in overall survival (OS), the degree of differentiation, depth of infiltration, pathological stage, vascular infiltration, nerve infiltration, fibrinogen, white blood cell, neutrophils, platelet, and platelet hematocrit (P<0.05). Tumor located in the middle thorax, larger lesion length, deeper invasion, later pathological stage, vascular infiltration, nerve infiltration, lymph node metastasis, cardiovascular disease, and higher smoking grade were risk factors for poor prognosis of esophageal squamous cell carcinoma (ESCC) (P<0.05). Cardiovascular disease, lower differentiation, tumor located in the middle thorax, and nerve infiltration were independent risk factors for the reduction of survival time of patients with ESCC (P<0.05). History of cardiovascular disease, tumor located in the middle chest, poorly differentiated esophageal squamous cell carcinoma, visible nerve cancer invasion, hematocrit (HCT), mean erythrocyte hemoglobin concentration (MCHC), and hemoglobin (HB) are independent risk factors for the long-term survival of patients with ESCC.

The Mechanism Underlying Lenalidomide-Induced Thrombosis Susceptibility

Introduction: Lenalidomide, a well-established drug for the treatment of multiple myeloma, significantly enhances patients' survival. Previous clinical studies have demonstrated that its main side effect is an increased risk of thrombotic events. However, the underlying mechanism remains unexplored. Therefore, this study aims to elucidate the mechanism and offer insights into the selection of clinical thrombotic prophylaxis drugs. Methods: Firstly, we conducted a retrospective analysis of clinical data from 169 newly diagnosed multiple myeloma patients who received lenalidomide. To confirm the impact of lenalidomide on thrombosis formation, FeCl3-induced thrombosis and deep venous thrombosis models in mice were established. To investigate the effects of lenalidomide on platelet function, both in vivo and in vitro experiments were designed. Results From January 2013 to June 2021, 169 NDMM patients with VRD as the first-line treatment were included in this study. During our follow-up, 8 patients had TEs . TEs included 8 VTE and 1 ATE. There were 7 DVT and 1 PE including in 8 VTE. Among the 8 patients suffering from thrombus, 2 patients did not receive thromboprophylaxis because of low platelet count, and the other 6 patients took aspirin, warfarin and rivaroxaban. Lenalidomide (25 mg/kg, 50 mg/kg, per day) or vehicle (DMSO) for 14 days were administrated for 2 weeks to healthy male mice. Compared with DMSO group, lenalidomide 25 mg/kg group and lenalidomide 50 mg/kg group significantly affected the formation of venous thrombosis , and there were significant statistical differences in both the length ( p=0.002; p=0.0005) and weight ( p&amp;lt;0.0001; p&amp;lt;0.0001) of thrombosis. In contrast, the formation of arterial thrombosis was not affected by lenalidomide administration in vivo, occlusion time of experiment group had control group did not have difference, which is consistent with the low incidence of ATE in clinical practice. To further explore the mechanism, we firstly focused on whether lenalidomide affects platelet function. Compared to baseline data, after taking one cycle of lenalidomide, the mean platelet volume (MPV, p&amp;lt;0.0001), platelet distribution width (PDW, p&amp;lt;0.0001), large platelet ratio ( p&amp;lt;0.0001), platelet hematocrit ( p=0.0005), and fibrinogen ( p=0.0008) of patients increased significantly. To evaluate the effect of lenalidomide on NDMM patient's platelet activation in vitro, we pretreated the washed platelets from NDMM with lenalidomide (5 or 10 um) or DMSO for platelet function assays . There was no difference between the three groups. To clarify the effect of lenalidomide on platelet function and parameters in vivo, lenalidomide (25 mg/kg, once per day), (50 mg/kg, once per day) or DMSO were administrated for 2 weeks to mice . The last dose was taken the night before narcotism, and platelet function was measured the next morning. We found that different from the changes in platelet related parameters in patients, in vivo data of mice showed no significant changes in platelet count , MPV , PDW , large platelet ratio , and platelet hematocrit after taking lenalidomide.Then we prepared washed platelets from mice for functional experiments. There was no difference between the three groups. In order to further explore the impact of lenalidomide on coagulation function in NDMM patients, we compared the coagulation parameters of NDMM patients who were enrolled at the time of diagnosis and after one cycle of taking lenalidomide. We found significant differences in prothrombin time, thrombin time, and prothrombin time ratio ( PTR, p&amp;lt;0.0001), while there was no statistical difference in some changes in activated partial thrombin time ( APTT, p=0.0881). These results may suggest that lenalidomide promotes the formation of venous thrombosis in vivo by influencing the coagulation pathway. Conclusions: The administration of lenalidomide had no significant impact on platelet function, which may affect venous thrombus formation by affecting coagulation. Therefore, anticoagulant drugs may be superior to antiplatelet drugs in the selection of clinical thrombus prophylaxis.

Clinical and Genetic Features By Next-Generation Sequencing and RNA Sequencing in Pre-Fibrotic Primary Myelofibrosis Patients

The 2016 WHO classification recognized pre-fibrotic primary myelofibrosis (pre-PMF) as a distinct entity. Such knowledge is essential to depict the landscape of pre-PMF and develop mechanism-based approaches to slow the progression of pre-PMF. Here, we conducted a retrospective study of 234 pre-PMF patients defined by 2016 WHO diagnostic criteria in 13 hematooncology centers between 01 January 2015 and 31 July 2023 (ChiCTR2200061208). We evaluated clinical characteristics, risk factors and genetic features of progression to overt-PMF in pre-PMF patients. Among 234 pre-PMF patients, 135 (57.7%) harbored JAK2V617F, 36 (15.4%) CALR and 3 (1.3%) MPL mutations; 60 (25.6%) patients were negative for all three mutations. JAK2V617F-mutated patients were older ( P=0.036), had more constitutional symptoms ( P=0.027) and cardiovascular risk factors ( P=0.045), higher white blood cell count (WBC) ( P&amp;lt;0.001), hemoglobin level (HB) ( P&amp;lt;0.001), neutrophile granulocyte (NEU) ( P&amp;lt;0.001), eosinophilic granulocyte (EOS) ( P&amp;lt;0.001), basophilic granulocyte (BAS) ( P&amp;lt;0.001), hematocrit (HCT) ( P=0.002) and red blood cell distribution width (RDW) ( P&amp;lt;0.001) (Figure A). CALR-mutated cases had higher platelet hematocrit (PCT) ( P=0.043) and lactic dehydrogenase (LDH) ( P=0.008). Besides, CALR group had a higher proportion of 25.0% to progress to overt-PMF than JAK2V617F group (9.6%) and TN group (6.7%) ( P=0.025) (Figure A). As for risk factors of progression to overt-PMF, univariable analysis found it was associated with mean platelet volume (MPV) ( P=0.019), HB ( P=0.027), WBC ( P=0.008), LDH ( P=0.004) and CALR mutations ( P=0.007). Multivariable analysis revealed that lower HB value ( P=0.041; HR 0.981, 95% CI 0.962-0.999) and CALR mutation ( P=0.001; HR 3.716, 95% CI 1.331-10.069) had negative impact on the progression to overt-PMF (Figure B). When divided by HB with cut-off point of 128 g/L, patients with HB value ≤128 g/L were more likely to progress to overt-PMF compared to &amp;gt;128 g/L groups ( P=0.007) (Figure C). And patients carried CALR mutation were also more likely to progress to overt-PMF ( P&amp;lt;0.001) (Figure D). We performed next-generation sequencing (NGS) in 18 pre-PMF patients, including 9 JAK2V617F, 3 CALR and 6 TN, 3 of which progressed to overt-PMF and 15 stayed in pre-PMF until the end of the follow-up. JAK2V617F was the most frequently mutated gene (9/38, 23.7%), followed by TET2 (7/38, 18.4%), RELN (4/38, 10.5%) and ASXL1 (3/38, 7.9%). JAK2V617F-mutated patients possessed more mutations related to activation signaling pathway ( P=0.025) and DNA methylation ( P=0.033) (Figure F). TN patients possessed more mutations related to transcription factor ( P=0.048) (Figure F). Patients who progressed to overt-PMF possessed more mutations associated with myeloid related transcription factor ( P=0.025) (Figure H). We further performed RNA-Seq analysis in 6 pre-PMF samples and 14 overt-PMF samples. Compared to pre-PMF, overt-PMF showed 49 up-regulated and 13 down-regulated differentially expressed genes (DEGs), of which CSCF3 and CXCL13 were significantly up-regulated (Figure I,J). Gene ontology (GO) functional enrichment analysis showed that DEGs were enriched in cell-cell signaling, cytokine activity, monocyte chemotaxis and chemokine activity (Figure K). KEGG biofunctional analysis showed that DEGs were significantly enriched in cytokine-cytokine receptor interaction, chemokine signaling pathway, IL-17 signaling pathway and NF-kappa B signaling pathway (Figure L). In conclusion, CALR-mutated pre-PMF patients with HB value ≤128 g/L were more likely to progress to overt-PMF. Genetic analysis found patients with high proportion of progress to overt-PMF tended to possess mutations associated with myeloid related transcription factor. And the upregulation of CSCF3 and CXCL13 may potentially drive the progression of overt-PMF in pre-PMF patients through inflammatory and cytokine related pathways. Acknowledgement: This research was funded by the Key R&amp;D Program of Zhejiang, No. 2022C03137; Public Technology Application Research Program of Zhejiang, China, No. LGF21H080003; Zhejiang Medical Association Clinical Medical Research special fund project, No. 2022ZYC-D09. *Correspondence to: Jian Huang, M.D., Ph.D., Department of Hematology, The First Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310003, Zhejiang, China. E-mail: househuang@zju.edu.cn

Blood cell differential count discretisation modelling to predict survival in adults reporting to the emergency room: a retrospective cohort study

ObjectivesTo assess the survival predictivity of baseline blood cell differential count (BCDC), discretised according to two different methods, in adults visiting an emergency room (ER) for illness or trauma over...

Clinical value of the platelet and inflammatory factor activation in vascular endothelial injury in essential hypertension.

To investigate the clinical value of platelet and inflammatory factor activation in vascular endothelial injury in hypertension. A total of 120 hypertension patients diagnosed in our hospital from December 2019 to June 2021 were enrolled as study objects (Hypertension group); besides, another cohort of 60 healthy people undergoing physical examination at the same period were recruited as the controls (Control group). Next, the baseline clinical characteristics of subjects in the two groups were recorded and compared. Specifically, a hematology analyzer was adopt for detecting the mean platelet volume (MPV), platelet distribution width (PDW) and platelet hematocrit (PCT); ELISA for the level of IL-6, IL-8 and TNF-α; PHILIPS EPIQ 7 C (a device assessing endothelial vasodilator function in a non-invasive fashion) for reactive hyperemia index (RHI); univariate and multivariate regression analysis for risk factors triggering endothelial dysfunction; and Spearman correlation analysis for the correlation of platelet activation indicators and inflammatory factor level with vascular endothelial function. Compared with the Control group, the patients in the Hypertension group exhibited higher levels of MPV, PDW, PCT, inflammatory factors (IL-6, IL-8 and TNF-α) and lower RHI. Moreover, Spearman correlation analysis showed a significant negative correlation of MPV, PDW, PCT, IL-6, IL-8 and TNF-α level with RHI level. In addition, univariate and multivariate regression analysis presented that MPV, PCT, IL-8 and TNF-α were risk factors for vascular endothelial dysfunction. The activation of platelet and inflammatory factor is closely related to vascular endothelial function injury in patients with hypertension. To be specifically, platelet and inflammatory factor activation can effectively reflect the vascular endothelial function injury in patients with hypertension and has high clinical value.

Effects of Galactomannan Oligosaccharides on Growth Performance, Mycotoxin Detoxification, Serum Biochemistry, and Hematology of Goats Fed Mycotoxins-Contaminated Diets.

This study was conducted to investigate the protective effects of mycotoxin adsorbent galactomannan oligosaccharides (GMOS) on growth performance, fermentation parameters, mycotoxins residues, serum biochemistry and oxidative stress parameters of the goats. The in vitro test indicated that 0.05% GMOS outperformed yeast cell wall (YCW) and montmorillonite (MMT) in aflatoxins absorption. Then 20 3-month-old Xiangdong black goats (15.0 ± 1.9 kg) were randomly divided into two dietary treatments for the animal test. The control group (CON group) was fed a multi-mycotoxins contaminated diet, whereas the experimental group (GMOS group) received multi-mycotoxins contaminated diet plus 0.05% GMOS. The trail lasted for 60 days, with 12 days of adaptation period and 48 days of formal experiment period. There were no treatment effects (P > 0.10) on growth performance, serum antioxidant capacity and activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). The concentrations of zearalenone in the rumen were lower (P < 0.05) in the GMOS group. GMOS significantly reduced (P < 0.05) propionate concentration in the cecum, resulting in a rise (P < 0.01) in acetate/propionate ratio in GMOS as compared to CON. Goats of GMOS exhibited considerably greater (P < 0.05) levels of creatine kinase but lower (P = 0.02) levels of creatinine than CON. Compared with CON, GMOS supplementation significantly increased (P < 0.05) platelet count (PLT), platelet volume distribution width (PDW), and platelet hematocrit (PCT), while decreased (P < 0.05) albumin content (ALB). The 0.05% GMOS protected goats in ruminal fermentation parameters, mycotoxins residues and serum biochemistry. Moreover, GMOS had no adverse effect on goat health. To our knowledge, this is the first report of GMOS in small ruminants. These findings suggested the feasibility of dietary GMOS as a health-maintaining addictive in goat diets.

Ambient particulate air pollution, blood cell parameters, and effect modification by psychosocial stress: Findings from two studies in three major Chinese cities

The associations between particulate matter (PM) exposure, psychosocial stress and blood cell parameters are bringing novel insights to characterize the early damage of multiple diseases. Based on two studies conducted in three Chinses cities using cross-sectional (Beijing, 425 participants) and panel study (Tianjin and Shanghai, 92 participants with 361 repeated measurements) designs, this study explored the associations between short-term exposure to ambient PM and blood cell parameters, and the effect modification by psychosocial stress. Increasing PM2.5 exposure was significantly associated with decreases in red blood cell (RBC) count and mean corpuscular hemoglobin concentration (MCHC), and increases in mean corpuscular volume (MCV), platelets count (PLT) and platelet hematocrit (PCT) in both studies. For instance, a 10 μg/m3 increment in PM2.5 concentration was associated with a 1.04% (95%CI: 0.16%, 1.92%) increase in PLT (4-d) and a 1.09% (95%CI: 0.31%, 1.87%) increase in PCT (4-d) in the cross-sectional study, and a 0.64% (95%CI: 0.06%, 1.22%) increase in PLT (1-d) and a 0.72% (95%CI: 0.33%, 1.11%) increase in PCT (1-d) in the panel study, respectively. In addition, stronger increases in MCV, PLT, and PCT associated with PM2.5 exposure were found in higher psychosocial stress group compared to lower psychosocial stress group (p for interaction <0.10), indicating that blood cell parameters of individuals with higher psychosocial stress might be more susceptible to the early damages of PM2.5 exposure.

Dengue Fever: Clinical, Laboratory Profile and Outcome in Pediatric Patient

Introduction: Infection with any of the 4 dengue virus serotypes results in a diverse range of symptoms, from mild undifferentiated fever to life-threatening hemorrhagic fever and shock. Given that dengue virus infection elicits such a broad range of clinical symptoms, early and accurate laboratory diagnosis is essential for appropriate patient management. So a study was carried out to know its clinical profile, correlation between the laboratory profile and the severity of dengue fever and outcome in dengue patients.&#x0D; Aim: To study the clinical profile, correlation between the laboratory profile and the severity of dengue fever and outcome in dengue patients at tertiary care center.&#x0D; Method: Retrospective Observational study from 1st May 2019 to 31st April 2021.&#x0D; Result: Total 323 patients were studied during 1st May 2019 to 31st April 2021. Most common presentation was fever (100%), most common clinical finding is hepatomegaly (14.2%). All severe dengue infection has platelet count &lt; 50000/cumm. In study of 323 patients 194(60%) of dengue fever,85(26.4%) of DHF GRADE 1,9(2.8%) of DHF GRADE 2 were discharged .13(4%) patients of DSS were expired.22 patients (6.8%) went DAMA.&#x0D; Conclusion: Reliable diagnosis of dengue fever in endemic areas can be done by clinical parameters like presence of nausea, vomiting, pain abdomen and hepatomegaly. Monitoring platelet count, hematocrit and WBC count is very useful for management of dengue cases.&#x0D; Keywords: dengue fever, platelet count, outcome

Risk factors associated with progression of diabetic retinopathy in eyes treated with panretinal photocoagulation

Uncontrolled diabetes has been associated with progression of diabetic retinopathy (DR) in several studies. Therefore, we aimed to investigate systemic and ophthalmic factors related to worsening of DR even after completion of panretinal photocoagulation (PRP). We retrospectively reviewed DR patients who had completed PRP in at least one eye with a 3-year follow-up. A total of 243 eyes of 243 subjects (mean age 52.6 ± 11.6 years) were enrolled. Among them, 52 patients (21.4%) showed progression of DR after PRP (progression group), and the other 191 (78.6%) patients had stable DR (non-progression group). The progression group had higher proportion of proliferative DR (P = 0.019); lower baseline visual acuity (P < 0.001); and higher platelet count (P = 0.048), hemoglobin (P = 0.044), and hematocrit, (P = 0.042) than the non-progression group. In the multivariate logistic regression analysis for progression of DR, baseline visual acuity (HR: 0.053, P < 0.001) and platelet count (HR: 1.215, P = 0.031) were identified as risk factors for progression. Consequently, we propose that patients with low visual acuity or high platelet count are more likely to have progressive DR despite PRP and require careful observation. Also, the evaluation of hemorheological factors including platelet counts before PRP can be considered useful in predicting the prognosis of DR.

PSIV-8 Effect of Feeding Levucell SB® 10 Titan Advantage and YANG on Performance, Blood Parameters, and Fecal VFA of Nursery Pigs

Abstract A total of 496 weaned pigs (19 ± 1 d of age) were used in a 3-phase feeding program to evaluate effects of Levucell SB 10 Titan Advantage, a live yeast, Saccharomyces cerevisiae boulardii (LEV; Elanco) and a non-viable yeast product (YANG; Elanco) on performance, complete blood count (CBC), and fecal VFA of nursery pigs. Pigs were blocked by weaning BW and gender and allotted to 44 pens (11–12 pigs/pen) which was assigned to one of 4 dietary treatments (11 pens/treatment). The study was designed as 2 x 2 factorial with LEV and YANG. Pigs were fed to meet or exceed nutrient requirements (NRC, 2012). Blood (d 10 and 22) and fecal samples (d 22) were collected from one pig per pen for CBC and VFA analyses. During d 0–7, feeding LEV or YANG alone improved (P &amp;lt; 0.05) G:F by 20% and 12%, respectively. There was a tendency of LEV*YANG interaction (P &amp;lt; 0.10) where feeding LEV increased (P &amp;lt; 0.05) BW and ADG compared to the control (CON). During d 0–14, feeding YANG with LEV reduced (P &amp;lt; 0.05) BW, ADG, and G:F compared to feeding YANG or LEV alone. Pigs fed LEV had an improved G:F compared to pigs fed CON or YANG with LEV (P &amp;lt; 0.05). During d 21–42, there was a tendency (P &amp;lt; 0.10) for a LEV*YANG effect where pigs fed CON had a lower G:F compared to other treatments. Overall, feeding LEV alone increased (P &amp;lt; 0.01) G:F (2.4%) and numerically increased final BW (0.60 kg) compared to CON. LEV tended to increase blood lymphocytes (P = 0.11) and increased platelets and platelet hematocrit (P &amp;lt; 0.05) compared to CON. There were no differences in fecal VFA measures. The results of this study indicate that feeding LEV increased G:F of nursery pigs.

Characteristics of autologous peripheral blood stem cells collection over a one-year period.

Aim To present characteristics of collecting autologous peripheral blood stem cells over a one-year period with an emphasis on efficiency and safety. Methods A retrograde analysis of 24 leukapheresis in 20 adult patients with malignant haematological diseases in the Blood Transfusion Institute of the Federation of Bosnia and Herzegovina in Sarajevo, was done. Cell separators Amicus and Spectra Optia were used for collection procedures. Results The patient's age ranged from 27 to 65 years. Target cells were collected in one procedure in sixteen patients, while in four patients they were collected in two procedures. The mean CD34+ collection efficiency was 57.7%. The median number of CD34+ cells and percentage of CD34+ cells in the products were 5.52x10e6/kg (range 3.28-9.00) and 1.57% (range 0.96-2.91). A strong positive correlation was found between the number of CD34+ cells in peripheral blood on the apheresis day and the amount of CD34+ cells collected in the products (rs =0.73). A total of 95% of patients collected the amount of ≥3x10e6/kg and 55% of ≥5x10e6/kg CD34+ cells for a single transplant. A decrease in platelet count, haemoglobin and haematocrit values after the procedure was not significant. Potassium decrease showed statistical significance (p<0.000). Adverse events occurred during one procedure (4.2%). Conclusion A low number of adverse events and good collection efficiency with adequate patient monitoring, indicate that leukapheresis is a safe procedure that is successfully used in the autologous transplantation process in the treatment of malignant haematological diseases.

Changes in and significance of platelet function and parameters in Kawasaki disease

Kawasaki disease (KD) is a systemic febrile, inflammatory vascular disease of unknown etiology. The coronary artery abnormality (CAA) caused by KD has become the most commonly acquired heart disease in children. Initial treatment of intravenous immunoglobulin (IVIG) can reduce the incidence of CAA. Thrombocytosis is common during the course of KD, but changes in and significances of platelet function and parameters are unclear. In this study, we enrolled 120 patients, including 40 patients with KD, 40 febrile controls, and 40 afebrile controls. The platelet function was assessed using the platelet function analyzer (PFA)-200. Platelet parameters, including platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), and platelet hematocrit (PCT) were measured. In the febrile period, the PDW and MPV were lower in KD patients (P < 0.05). The platelet function did not change significantly during the febrile period of KD but weakened in the defervescence phase. No significant differences between the CAA and normal groups, and between IVIG resistance and response groups. The diagnostic cutoff value of the PDW level for predicting KD was 10.85 fL with a sensitivity of 55% and a specificity of 77.5% (area under curve (AUC) = 0.690, 95% confidence interval (CI): 0.574–0.806, P < 0.01). Besides, the MPV level was 9.55 fL with sensitivity of 75% and specificity of 70% (AUC = 0.733, 95%CI: 0.620–0.846, P < 0.001). This is the first longitudinal study of platelet function changes in KD patients using PFA-200. Besides, lower PDW and MPV may be available markers for early diagnosis of KD.

Prevalence of dengue-related fundus and macular optical coherence tomography findings among inpatients in a regional referral hospital

Purpose: To investigate the prevalence of fundus and macular optical coherence tomography (OCT) findings, and the spectrum of dengue-related fundus presentation in a Malaysian tertiary hospital. The associations between platelet count and haematocrit level with fundus and macular OCT findings were also investigated.&#x0D; Design: Cross-sectional study.&#x0D; Methods: The study was conducted at Hospital Raja Permaisuri Bainun, Ipoh, from June to August 2015. Patients who consented to participate underwent a comprehensive ocular examination. Examination included a best-corrected distance (6 m) and near visual acuities, standard black-on-white Amsler chart testing, pupillary light reflex, fundus examination, followed by dilated fundus photographs and OCT of the macula.&#x0D; Results: A total of 134 patients were included in the study. The prevalence of positive fundus finding and macular OCT finding was 35% (95% confidence interval [CI]: 27%, 43%) and 13% (95% CI: 8%, 19%), respectively; 62 eyes of 47 patients had positive fundus findings, whereas 30 eyes of 18 patients had positive macular OCT findings. Scotoma (p &lt; 0.001), near vision disturbance (p = 0.04), and abnormal Amsler findings (p &lt; 0.001) were significantly associated with presence of macular OCT findings compared to absence of macular OCT findings. In the total of 268 eyes, the two most common fundus findings were vessel tortuosity (53 [20%]) and yellow subretinal dot (28 [10%]). Out of 30 eyes, diffuse retinal thickening was the most frequent OCT finding (22 [73%]), followed by 4 (13%) with foveolitis, 3 (10%) with cystoid macular oedema and 1 (3%) with submacular fluid. Platelet count and haematocrit were not associated with abnormal fundus or macular OCT manifestation in patients suffering from dengue fever.&#x0D; Conclusion: Our study revealed that the prevalence of clinical fundus and macular OCT findings among dengue inpatients was higher when compared to other countries, especially during dengue outbreaks. Furthermore, the spectrum of fundus and macular OCT findings in our population can be varied.

Взаимосвязь эритроцитарных, тромбоцитарных показателей и гематокрита в крови с характером иммунной реакции человека на кратковременное общее охлаждение

Взаимосвязь эритроцитарных, тромбоцитарных показателей и гематокрита в крови с характером иммунной реакции человека на кратковременное общее охлаждение

Thrombosis Risk with High Platelet Counts and Hematocrit Values

The increased risk for thrombosis in patients with myeloproliferative neoplasms, including essential thrombocytosis and polycythemia vera, is well

Peripheral Blood miR-451 May Serve as a Biomarker of Ischemic Stroke.

The current study aims to investigate the correlation between the expression level of miR-451 in peripheral blood and the risk of ischemic stroke, and its feasibility as a biomarker of ischemic stroke. Three hundred and two cases of ischemic stroke diagnosed in Qianfoshan Hospital Affiliated to Shandong University from April 2017 to Dec 2017 and 302 cases matched for age and gender were selected from routine health examination subjects. Real-time quantitative PCR was used to detect the expression of microRNA in peripheral blood. Receiver operating curve (ROC) was used to analyze whether miR-451 could be used as a basis for judging ischemic stroke. The expression level of miR-451 in peripheral blood of patients with ischemic stroke was higher than that of the control group (p < 0.05). ROC analysis demonstrated that peripheral blood miR-451 could screen ischemic stroke patients from healthy controls, with the AUC of 0.912. In addition, the expression level of miR-451 was negatively correlated with the number of platelets and platelet hematocrit (p < 0.05). There is a significant correlation between the expression level of miR-451 in peripheral blood and the occurrence of ischemic stroke. miR-451 is expected to be a biomarker of ischemic stroke.

Clinical and hematological profile in dengue

Background: Dengue is the most rapidly spreading mosquito-borne viral disease. The spectrum ranges from a nonspecific febrile illness to severe disease, i.e., dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) with the development of hematological complications. Since the death in these patients is due to hematological complications, their study would have a substantial impact on reducing the mortality and morbidity associated with dengue. Objective: The objective of this study was to evaluate the hematological changes in serologically positive dengue patients and to correlate the same with different stages of serology and clinical outcome. Materials and Methods: Evaluation of the hematological parameters and peripheral smear study in clinically suspected cases of dengue with serological confirmation was carried out. Clinical data along with the outcome were collected from medical records. Chi-square test, somatosensory evoked potential, and logistic regression analysis were applied to analyze the results. Results: Three hundred and forty-eight serologically positive dengue cases were analyzed. The disease manifested as dengue fever (DF) in 83.5% of cases, DHF in 10.6% of cases, and DSS in 4.9% of cases. Thrombocytopenia was the most common hematological finding followed by anemia, leukopenia, leukocytosis, and increased hematocrit. Peripheral smears showed atypical lymphocytes, neutrophilic toxic granules, giant platelets, and granulocytic shift to left. About 94.26% of patients recovered and 3.45% had fatal outcome. Conclusion: Platelet count and hematocrit play a crucial role in predicting prognosis of DHF and DSS. Thrombocytopenia was associated more with DHF and DSS than DF. There was a significant association between decreased platelet count and mortality rate.

Correlation between platelet related parameters and acute rejection in renal transplant recipients

Objective To investigate the relationship between primary platelet parameters and early acute rejection (AR) after renal transplantation. Methods From October 2015 to June 2017, 112 patients with renal failure underwent allograft renal transplantation in the Second People's Hospital of Shanxi Province were selected as the research objects, according to the preoperative and postoperative 30d in platelet parameters based on the occurrence of AR they were classified into the observation group (23 cases), no rejection for the control group (89 cases). analysis the difference of two groups of patients with platelet parameters [peripheral blood platelet count (PLT), platelet hematocrit (PCT), mean platelet volume (MPV) and platelet volume distribution width (PDW), the ratio of large platelet (P-LCR)] at preoperative (T0), postoperative 5 d (T1), 10 d (T2) after operation, postoperative 15 d (T3) 15, 30 d (T4). The correlation between PLT, PCT, MPV, PDW, P-LCR and acute rejection after renal transplantation was also analyzed. Results Compared with those of preoperative, the PLT[(122.06±49.67)×109/L vs.(185.26±63.49)×109/L)], PCT[(0.46±0.28)% vs.(0.34±0.06)%)] in T1, T2 of the two group after surgery declined (P<0.05). MPV, PDW and P-LCR were increased in both groups after surgery (P<0.05), and the observation group were higher than that in the control group MPV[(13.69±0.59) vs.(12.24±0.42), (16.08±0.61) vs.(12.52±0.57)], PDW[(13.26±1.19) vs.(12.13±2.14), (15.46±3.15) vs.(12.72±2.09)], P-LCR[(30.57±7.29) vs.(28.13±6.34), (33.01±3.98) vs.(29.13±6.91)] at the baseline of T1 and T2 (all P<0.05). Correlation analysis showed that the level of MPV , PDW , P-LCR and the occurrence of acute rejection after kidney transplantation were positively correlated (r=0.464, 0.606, 0.512, all P<0.05), while the PLT, PCT has nothing to do with the occurrence of acute rejection after kidney transplantation. Conclusion MPV, PDW and P-LCR can indirectly reflect the occurrence of AR after renal transplantation, and the trend of monitoring the three indexes of MPV, PDW and P-LCR after operation is an auxiliary diagnostic method for postoperative AR. Key words: Kidney transplantation; Kidney failure, chronic; Blood platelets; Graft rejection