Plastic Tip Research Articles

In Vitro Comparison of Titanium Disc Surface Roughness and Bacterial Colonization After Ultrasonic Instrumentation With Three Different Tips.

During implant maintenance, preserving a smooth surface on the machined transmucosal abutment is critical to reduce biofilm attachment and colonization. The present study compared the surface roughness and bacterial colonization of machined titanium surfaces after instrumentation with various materials. Forty-four machined grade 23 titanium discs were instrumented with a round polyether ether ketone (PEEK) tip, a plastic curette tip, or a pure titanium curette tip with piezoelectric devices. Before and after instrumentation, the surface roughness (Ra and Rz) values were analyzed with a profilometer and scanning electron microscopy (SEM). Streptococcus sanguinis was cultured and incubated for 24 hours on the instrumented discs, and colony-forming units per milliliter were obtained for each group. Samples instrumented with the metal ultrasonic tip significantly increased surface roughness compared with the other groups. This resulted in greater colonization by S. sanguinis than surfaces instrumented with PEEK tips or the negative control. Samples instrumented with PEEK and plastic tips did not exhibit any statistically significant increase in surface roughness, and SEM analysis revealed a significantly rougher surface of discs instrumented with metal compared with discs instrumented with plastic or PEEK tips despite the possibility of debris from tip dissolution. Our results suggest that instrumentation with metal ultrasonic tips with piezoelectric devices significantly increased machined titanium's surface roughness and elicited higher biofilm formation in vitro. Meanwhile, instrumentation of machined titanium with PEEK or plastic ultrasonic tips did not affect the surface roughness or bacterial adhesion.

Development of Plastic Zone Near Tip of Interfacial Crack with Contacting Faces

Development of Plastic Zone Near Tip of Interfacial Crack with Contacting Faces

Paper-in-Tip Bipolar Electrospray Mass Spectrometry for Real-Time Chemical Reaction Monitoring.

Capturing short-lived intermediates at the molecular level is key to understanding the mechanism and dynamics of chemical reactions. Here, we have developed a paper-in-tip bipolar electrolytic electrospray mass spectrometry platform, in which a piece of triangular conductive paper incorporated into a plastic pipette tip serves not only as an electrospray emitter but also as a bipolar electrode (BPE), thus triggering both electrospray and electrolysis simultaneously upon application of a high voltage. The bipolar electrolysis induces a pair of redox reactions on both sides of BPE, enabling both electro-oxidation and electro-reduction processes regardless of the positive or negative ion mode, thus facilitating access to complementary structural information for mechanism elucidation. Our method enables real-time monitoring of transient intermediates (such as N,N-dimethylaniline radical cation, dopamine o-quinone (DAQ) and sulfenic acid with half-lives ranging from microseconds to minutes) and transient processes (such as DAQ cyclization with a rate constant of 0.15 s-1). This platform also provides key insights into electrocatalytic reactions such as Fe (III)-catalyzed dopamine oxidation to quinone species at physiological pH for neuromelanin formation.

Paper‐in‐Tip Bipolar Electrospray Mass Spectrometry for Real‐Time Chemical Reaction Monitoring

AbstractCapturing short‐lived intermediates at the molecular level is key to understanding the mechanism and dynamics of chemical reactions. Here, we have developed a paper‐in‐tip bipolar electrolytic electrospray mass spectrometry platform, in which a piece of triangular conductive paper incorporated into a plastic pipette tip serves not only as an electrospray emitter but also as a bipolar electrode (BPE), thus triggering both electrospray and electrolysis simultaneously upon application of a high voltage. The bipolar electrolysis induces a pair of redox reactions on both sides of BPE, enabling both electro‐oxidation and electro‐reduction processes regardless of the positive or negative ion mode, thus facilitating access to complementary structural information for mechanism elucidation. Our method enables real‐time monitoring of transient intermediates (such as N,N‐dimethylaniline radical cation, dopamine o‐quinone (DAQ) and sulfenic acid with half‐lives ranging from microseconds to minutes) and transient processes (such as DAQ cyclization with a rate constant of 0.15 s−1). This platform also provides key insights into electrocatalytic reactions such as Fe (III)‐catalyzed dopamine oxidation to quinone species at physiological pH for neuromelanin formation.

Aptamer based hybrid monolithic pipette tips supported by melamine sponge for enrichment of proteins

BackgroundThe convenient preparation and application of functionalized organic-inorganic hybrid monolithic materials have obtained substantial interest in the pretreatment of complex samples by solid-phase extraction (SPE). Compared to the in-tube solid-phase microextraction in fused-silica capillaries, micro SPE in plastic pipette tips have fascinating merits for the easily operated enrichment of trace target analytes from biological samples. However, the poor compatibility of organic-inorganic hybrid monoliths with plastics leads to the rare appearance of commercial hybrid monolithic pipette tips (HMPTs). Therefore, how to synthesize the organic-inorganic hybrid monolithic materials with better extraction performance in plastic pipette tips becomes a challenge. ResultsWe develop a facile and cheap strategy to immobilize organic-inorganic hybrid monoliths in pipette tips. Melamine sponge was employed as the supporting skeleton to in situ assemble amine- and thiol-bifunctionalized hybrid monolithic material via “one pot” in a pipette tip, and gold nanoparticles (GNPs) and thiol-modified aptamer against human α-thrombin were sequentially attached to the hybrid monolith within the HMPTs. The average coverage density of the aptamer with GNPs as an intermediary reached as high as 818.5 pmol μL−1. The enriched thrombin concentration was determined by a sensitive enzymatic chromogenic assay with the limit of detection of 2 nM. The extraction recovery of thrombin at 10 nM in human serum was 86.1 % with a relative standard deviation of 6.1 %. This proposed protocol has been applied to the enrichment and determination of thrombin in real serum sample with strong anti-interference ability, low limit of detection and high recovery. SignificanceThe amine- and thiol-bifunctionalized HMPTs prepared with sponge as the skeleton frame provided a novel substrate material to decorate aptamers for efficient enrichment of proteins. This enlightens us that we can take advantage of the tunability of sponge assisted HMPTs to produce and tailor a variety of micro SPE pipette tips for broader applications on the analysis of trace targets in complex biological, clinic and environmental samples.

Mixed-mode crack growth analysis using a cyclic plasticity model

The effects of two singularity fields, namely, the HRR solution and modified form of the nonlinear kinematic hardening (NKH) solution, on the pure mode I and mixed mode I/II fatigue crack propagation were investigated. Experiments and computations were performed on compact tension shear (CTS) specimens of P2M steel, aluminium 7050, and Ti-6Al-4V with different monotonic and cyclic elastic–plastic properties. The analysis of the experimental data on the crack growth rate for all tested materials is given in terms of plastic stress intensity factors (SIFs) according to the HRR and NKH models. The elastic–plastic crack tip fields were obtained by FE computation as a function of the position along the curvilinear crack path for the monotonic condition and the first several cycles of fatigue deformation considering the Bauschinger effect. As a complement to the FE modelling, digital image correlation (DIC) measurements of the displacement and strain fields around the crack tip in the CTS specimens were performed. This study aimed to obtain more detailed information on the local cyclic strain ahead of the mixed mode crack tip by using DIC. From the sensitivity analysis, it was established that the DIC value depended significantly on the location along the crack direction of the pair of points selected before and behind the crack tip. In this study, acceleration or retardation of the crack growth rate in terms of the NKH plastic SIF with respect to the HRR model was observed because of the coupling effect of the mode mixity and cyclic plastic properties of the tested P2M steel, aluminium 7050, and Ti-6Al-4V.

Auto-Lectin Dotcoding by Two Octopuses: Rapid Analysis of Fluorescence-Labeled Glycoproteins by an 8-channel Fully-Automatic Bead Array Scanner with a Rolling-Circle Detector.

Protein glycosylation is a crucial factor that must be evaluated in biological pharmaceuticals. The glycoform profile of a protein can vary depending on the conditions of the cultivation, purification process, and the selection of a host cell. Lectin microarrays are reliable bioanalytical methods used in the early phases of bioprocesses for the detection of glycosylation. The concept of a fully automated glycan detection with a bead array has been previously reported; however, no simple system has been constructed on fluorescence-based detection using a microarray. Here, we present a fully automated detection system equipped with a novel fluorescence detector for a 13-lectin bead array with a single tip. The lattice-like arrangement of a set of fibers proximate to the tip of the light emitting diode and photomultiplier tube detector minimized the noise caused by the reflection of incident light on the plastic capillary tip and bead. A unique rolling-circle fiber unit with quadruple lattices stacked in two layers realizes the 8-parallel automeasurement with a drastic reduction in scanning time and machine size. The 8-glycan profiles obtained automatically within 25 min were identical with those obtained with the conventional lectin microarray after overnight incubation. The signals obtained were represented as lectin dotcodes. Therefore, autolectin dotcoding assisted by the twin 8 legs named as "detection and irradiation octopuses" may be a rapid glyco-evaluation system during the production and development of biopharmaceuticals.

Extending the Lifetime of Copper-beryllium Alloys as Plastic Injection High-end Needle Valve Mold Nozzle Tips Through a Heat-treatment-based Microstructure Optimization Approach

Extending the Lifetime of Copper-beryllium Alloys as Plastic Injection High-end Needle Valve Mold Nozzle Tips Through a Heat-treatment-based Microstructure Optimization Approach

Blunting and wear of AFM tips during dynamic lithography

Mechanical scanning probe lithography using AFM tips has been applied to the fabrication of nanostructures on material surfaces. Particularly, the dynamic lithography under the AFM tapping mode is promising in fabricating the surface nanostructures at the width of around 20 nm. However, tip blunting and gradual atom-by-atom wear occurred during the process although the tip tapping motion has largely reduced the tip-surface interaction force and time. This study aims to investigate the blunting rate and mechanism of such AFM tips. It was found that the maximum contact stress was in the range of 0.7 to 1.0 GPa when a silicon AFM tip (initial tip radius under 20 nm) was tapped on a PMMA thin-film surface. And the contact stress was mainly attributed to the tip radius, which was the key factor to limit machinability and machining efficiency of the dynamic lithography. Furthermore, the mechanism of the tip blunting was through its plastic deformation and tip wear. A stress analysis indicated that it was the large stress gradient that contributed to the high density of wrinkles in the tips, and that ninety percent of the blunting of a tip were caused by the plastic deformation, leading to a significant increase of the tip radius.

Resolution of peri-implant mucositis at tissue- and bone-level implants: A 6-month prospective controlled clinical trial.

The objective of the study was to compare resolution of inflammation of naturally occurring peri-implant mucositis (PM) at tissue-level (TL) and bone-level (BL) implants after non-surgical mechanical debridement. Fifty-four patients with 74 Implants with PM were allocated in two groups (39 TL and 35 BL implants) and treated by means of subgingival debridement using a sonic scaler with a plastic tip without adjunctive measures. At baseline and at 1, 3, 6months, the full-mouth plaque score (FMPS), full-mouth bleeding score (FMBS), probing depth (PD), bleeding on probing (BOP), and modified plaque index (mPlI) were recorded. The primary outcome was BOP change. After 6months, the FMPS, FMBS, PD, and number of implants with plaque decreased statistically significantly in each group (p < .05); however, no statistically significant differences were found between TL and BL implants (p > .05). After 6months, 17 (43.6%) TL and 14 (40%) BL implants showed a BOP change in (17.9%) and (11.4%), respectively. No statistical difference was recorded between groups. Within the limitations of present study, the findings showed no statistically significant differences in terms of changes in clinical parameters following non-surgical mechanical treatment of PM at TL and BL implants. A complete resolution of PM (i.e., no BOP at all implant sites) was not achieved in both groups.

Electrochemical detection of copper(II) in environmental samples using Penicillium sp. IITISM_ANK1 based biosensor

Detection of toxic metals at the lowest of their concentration in a variety of matrices has become a necessity due to the widespread and persistent nature of the contaminant. In this context, biosensors provide a viable alternative to the large sophisticated instrumentation. This study focuses on the development of a cost-effective fungal biomass-based biosensor that can be used for the detection of Cu(II). The fungal cells were pretreated with formaldehyde and studied with various spectroscopic studies. It was observed that the carbonyl groups along with amine groups played role in the sorption of copper ions which were stripped in an electrolytic solution to quantify the metal. Herein the dried fungal biomass was chemically activated and used to prepare a novel graphite paste electrode by repurposing a plastic pipette tip. The factors affecting the detection signal were optimized in further study. The electrochemical characterization revealed that the prepared bio-electrode was capable of detecting Cu in the range of 1 × 10−7 M to 2 × 10−5 M.

Coated Blade Spray with a Barrier: Improving Negative Electrospray Ionization and Sample Preparation.

Substrate-based electrospray ionization (ESI) techniques like paper, wooden tip, plastic tip, and metal-needle-based spray suffer from corona discharge, high background noise, and unstable spray in negative ionization mode, especially for the analysis of complex biological matrices, such as blood and urine. Coated blade spray coupled with mass spectrometry (CBS-MS) combines solid-phase microextraction's (SPME) efficient sample clean-up and enrichment and ambient MS's fast analysis and has proven to be an appealing alternative tool for the fast screening of target analytes in complex matrices. This paper documents the development of a new CBS blade design that features a barrier at the far end of the ESI tip. The findings of this work show that the addition of this simple barrier enabled the total RSD% to be reduced to less than 10% for sample preparation, ionization, and the MS detection of several drugs of abuse in negative mode, without compensation using internal standards. The improved stability of ESI in negative mode was investigated by observing the ESI process with a microscope camera and testing via CBS-MS. The new design was applied for the analysis of three drugs of abuse in urine, with the calibration curve correlation coefficient (R2 ≥ 0.9997) being calculated without the use of internal standards. The overall RSD% of the peak area for one compound in 42 samples was 6.9%, which highlights the method's incredible reproducibility compared to other ambient MS techniques for analyzing real samples. The CBS device with a barrier was also applied for the on-blade sampling of 14 drugs of abuse in 20 μL of plasma spot in positive ionization mode. The results of these tests yielded a calibration curve correlation coefficient of R2 ≥ 0.9883 and limits of quantification (LOQs) between 0.25 and 25 ng/mL. The obtained results provide guidance on CBS device design optimization and the effective automation of the protocol.

Influence of some metabolites from plants of the genus &lt;i&gt;Petasítes&lt;/i&gt; sp. on cancer cells motility in vitro

Secondary plant metabolites are a promising source of anticancer drugs. Of particular interest are substances that can reduce the migration activity of cancer cells as potential anti-metastatic drugs. In present work we study the influence of 2,4-dihydroxy-2,5-dimethylfuran-3(2H)-one, 5-(hydroxymethyl)furan-2-carbaldehyde and corinan obtained from plants of the genus Petastes sp. on scratch healing rate of permanent cancer lines PC3, A431, CaCo2, HeLa, and T98G. Cells were grown in DMEM medium supplemented with 10% FBS. To perform a scratch test, cells were planted in an amount of 1,5105 per well of a 24-well plate. After cell adhesion, a vertical scratch was applied to the cell monolayer with a plastic tip, after which the medium containing the test substances at a concentration of 40 M was added. Then, within 48 hours, photographing and determining the scratch area were carried out. The degree of scratch overgrowth was determined as the ratio of the difference between the scratch areas after 48 hours of cultivation and the scratch area at the initial moment, expressed as a percentage. As a result of the experiment, it was shown that 2,4-dihydroxy-2,5-dimethylfuran-3(2H)-one downregulates scratch healing rate in cultures A431, HeLa, T98G. The high sensitivity of A431 to corinan was also shown.

Fatigue crack growth modelling for S355 structural steel considering plasticity-induced crack-closure by means of UniGrow model

The UniGrow model is an analytical procedure to assess the fatigue crack growth based on elastic–plastic crack tip stresses and strains. The assumption is that fatigue crack growth (FCG) can be considered as a process of successive crack re-initiations resulting from material damage in the crack tip zone. The main parameters of this model are the crack tip radius and the elementary block size. Experimental FCG data obtained for S355 carbon steel showed that assuming the elementary block size with the same value of the crack tip radius to collapse FCG data using UniGrow model is non-coherent with experimental evidence. In this sense, a new approach is proposed by establish a clear distinction between crack tip radius and elementary block size. The value of the crack tip radius, ρ, was defined by correlation with experimental and numerical values of residual compressive stress field ahead of the crack tip while for the elementary block size, ρ∗, a new expression was proposed which relies on effective stress intensity factor range and cyclic yield strength. This research intends to be a valuable contribution for the implementation of UniGrow model to assess the fatigue crack growth of a material.

Add-on Plastic Tips for Cryogen Administration in Difficult-to-access Areas.

Targeted cryotherapy of lesions in difficult to access location is a challenging scenario. We propose the use of add-on tips fashioned from disposable micropipettes, needle caps, and syringe barrels as a solution.

The Valero Tip and Attachment for Cooper Surgical Rumi Uterine Manipulator

<h3>Study Objective</h3> To produce a Stainless-Steel surgical degree tip and attachment that works with uterine manipulator and allows the use of cervical cup for different types of surgeries. - Plastic pollution continue to be a serious problem for human health and environment, it's estimate that for 2030, 53 million tonnes of plastic could end up in rivers, lakes and oceans. One example of how changing consumption patterns can improve the environment is the use and disposal of plastics. The global theme of World Environment Day is "Beat Plastic Pollution". It aims to change consumer behaviour with the challenge, "If you can't reuse it, refuse it." In OR we don't have exception, for reducing Medical Waste and optimizing our ORs for the future, we design a stainless-steel surgical degree tip and attachment for the uterine manipulator. <h3>Design</h3> We produce a 3-D Model plastic tip as a prototype, subsequently a stainless-steel tip and attachment <h3>Setting</h3> Like it adapts to a uterine manipulator, patient position is lithotomy, the Tip attaches by a "ring" then allows the use of a cervical cup that adapts to the ring. <h3>Patients or Participants</h3> N/A. <h3>Interventions</h3> N/A. <h3>Measurements and Main Results</h3> The design patent pending through the School of Medicine and Engineer, attach to the three types of uterine manipulator also it allows to use the cervical cup and provides the ability to use it in hysterectomy or other procedures without the cervical cup. Base on this we present the prototype guide and assemble. <h3>Conclusion</h3> The reestirilization of the tip allows us not promote the use of plastic waste.

Resin Nano-Ceramic Material Surface Roughness, Color Stability, and Bacterial Adhesion Evaluation After Different Scaling Methods

Objectives: The study evaluated in vitro the changes in roughness, color stability, and bacterial count of a CAD/CAM Resin Nano-Ceramic material surface treated by various scaling procedures. Material and Methods: 70 disks (5mm diameter, 0.5 mm thickness) of Resin Nano-Ceramic (Lava ™Ultimate, 3M, ESPE) material were cemented in standardized cavities prepared in bovine teeth. A custom-made scaling apparatus of a double pan balance was used for different scaling methods, simulating standard clinical conditions. The specimens were assigned to three main groups: no scaling(C), ultrasonic scaling (U), and manual scaling (M). Each group was then divided into three subgroups according to scaling tip material; stainless steel tip (St), plastic tip (P), and titanium tip (Ti). The surface texture was analyzed quantitatively and qualitatively with a tactile profilometer and atomic force microscopy. A spectrophotometer was used for color measurement. Streptococcus mutans were counted in a colony counter. All the data were tabulated and statistically analyzed. Results: Two-way ANOVA was used to study the effect while One-way ANOVA was performed to compare between study groups. The significance level was set at p 0.05. The ultrasonic titanium tip(UTi) revealed the significant highest mean value of alterations (p &lt; 0.001). The integrity of the material surface was altered in the form of deep scratches on the ultrasonically scaled surfaces and numerous smaller scratches on the hand-scaled surfaces. Conclusion: The plastic instrument would appear to be the instrument of choice during a routine maintenance procedure for Resin Nano-Ceramic materials. Keywords Bacterial biofilm; Dental ceramics; Color stability; Scaling; Surface roughness.

An in vitro evaluation of surface roughness, color stability and bacterial accumulation of lithium disilicate ceramic after prophylactic periodontal treatment

Objective: to evaluate the effect of scaling procedures using different ultrasonic tips on the surface roughness, color stability and bacterial accumulation of lithium disilicate ceramic. Material and Methods: Scaling procedure was carried out using ultrasonic scaler (Satalec, Acteon, North America) with stainless-steel tip (US), titanium tip (UT) and plastic tip (UP), on disc shaped lithium disilicate samples cemented into a cavity prepared onto the labial surface of freshly extracted bovine teeth (10 samples per group). The samples were stored in coffee solution in an incubator at 37°C for 12 days, which is equivalent to 1 year of coffee consumption. The surface roughness was measured before and after the scaling procedure using a profilometer and atomic force microscopy. The color parameters were measured before and after scaling and staining procedures using VITA Easyshade Advance 4.0 according to the CIE L*a*b* color order system. The samples were then incubated with Streptococcus mutans (S. mutans) suspension. After incubation, the plates with 30 to 300 typical colonies of S. mutans were counted in a colony counter and mean values of colony forming units were obtained (CFU/mL). Results: The titanium scaling tip showed a statistically significant higher mean values of change in surface roughness Ra and bacterial count than the plastic scaling tip. Color changes (E) were not a statistically significant among the groups. The results showed a statistically significant positive (direct) correlation between surface roughness and color change (p = 0.012) and also between surface roughness and bacterial count (p = 0.00). Conclusion: Within the limitations of this study, titanium scaling instruments cause irreversible surface alterations of lithium disilcate ceramics which was in direct correlation to the color changes and bacterial accumulation; therefore, dentists should proceed with caution when scaling lithium disilicate surfaces. The findings of the current study may indicate the need for instruments or equipment that can remove plaque and calculus without causing surface damage. Keywords Surface properties; Color; Bacterial adhesion; Ultrasonics; Dental scaling; Ceramics.

A detailed study of mode II test specimen with elastic or plastic crack tips by using boundary element method

In linear elastic fracture mechanics the stress and strain magnification at the crack tip is characterized by stress intensity factors which have been obtained for a large variety of loading and specimen geometries and of course mode II test specimen included. In this paper not only different kinds of boundary conditions of the contact surfaces of the elastic crack tip will be discussed but the plastic one. The rotation of the center line of the specimen will also be discussed. The results show the powerful application of the BEM model in the specimen.

Impact of Tyrosine Kinase Inhibitors Used in Chronic Myeloid Leukemia on Signaling and Epigenetics in Endothelial Cells

Background: Tyrosine kinase inhibitors (TKIs) have revolutionized treatment of chronic myeloid leukemia (CML) resulting in long term survival with chronic therapy. Vascular toxicities have emerged as complications of the second generation CML TKIs; dasatinib, ponatinib and nilotinib. Dasatinib is associated with increased risk of pulmonary hypertension, ponatinib with arterial ischemic events and nilotinib with peripheral arterial occlusive events and cardiovascular ischemia by unknown mechanisms. The first generation CML TKI imatinib is not associated with vascular toxicity.Hypothesis: We hypothesize that the CML TKIs- ponatinib, dasatinib and nilotinib, but not imatinib, cause vascular toxicity by affecting endothelial cell (EC) function in association with alterations in phospho-signaling and histone modifications.Methods To test this hypothesis, we studied the effect of CML TKIs imatinib, nilotinib, dasatinib and ponatinib on EC function using three in-vitro assays measuring EC survival, EC-leucocyte adhesion (a measure of vascular inflammation) and scratch wound closure (a measure of wound healing capacity). Cultured human umbilical vein ECs (HUVECs) were treated with vehicle, imatinib (4uM), dasatinib (200nM), nilotinib (3 uM) or ponatinib (100nM). Cell viability was measured at 18 hours using a Cell TiterGlo Luminescent Cell Viability assay (Promega). For the leucocyte adhesion assay, fluorescent labeled leucocytes (U937) were incubated with the ECs and adherent fluorescent cells were quantified. For the wound healing assay, a scratch “wound” was made with a plastic P1000 pipette tip and cells that migrated into the wound area were counted at 18 hours. To assess signaling, two novel, global, unbiased, high throughput proteomic assays developed at the Broad Institute were used. The P100 assay is a mass spectrometry-based targeted proteomics assay that quantifies 100 representative phosphopeptides. The global chromatin profiling (GCP) assay uses mass spectrometry to simultaneously quantify over 70 bulk histone modifications including phosphorylation, ubiquitination, methylation and acetylation. HUVECs were treated in triplicate with each drug for 3 hours for the P100 assay and for24 hours for the GCP assay. For each assay, the peptides were mixed with isotopically labeled internal standards and mass spectrometry was performed to quantify each phospho-peptide or modified histone peptide relative to the universal standard.Results: The CML TKIs that are associated with vascular toxicity (nilotinib, ponatinib and dasatinib) negatively impacted all the EC functions measured. Specifically, nilotinib, ponatinib and dasatinib significantly decreased endothelial cell viability and endothelial cell migration in response to a scratch wound and increased leucocyte adhesion to ECs. Imatinib did not have a negative effect on ECs in any of those assays. Using the mass spectrometry based P100 and GCP assays, we found that nilotinib affects histone epigenetics and protein phosphorylation in HUVECs. In particular, nilotinib treatment modulates ABL1, MAP4, BRD4 and JUND phosphorylation and also increases histone H3K9 trimethylation with H3S10 phosphorylation in HUVECS. We also compared the nilotinib profiles obtained in HUVECs to that of primary human aortic smooth muscle cells (HAoSMC) and several cancer cell lines including MCF7 (breast), A549 (lung), A375 (skin) and PC3 (prostate) (Figures 1a and 1b). While some changes are unique to ECs, others are similar to the effects in cancer cells and smooth muscle cells. GCP assay and P100 assays comparing the effects of all the CML TKIs in endothelial cells are in process.Conclusions: CML TKIs associated with vascular toxicity affect human EC function in vitro resulting in impaired endothelial survival and wound healing and increase inflammation. These changes are associated with alterations in signaling and epigenetic pathways in ECs in response to treatment with nilotinib. Increased vascular inflammation and impaired vascular healing are associated with cardiovascular thrombosis and ischemia. Further studies comparing the effects of all CML TKIs on ECs will be used to elucidate molecular mechanisms of vascular toxicity and help devise strategies to overcome these toxicities while maintaining anti-cancer efficacy. [Display omitted] DisclosuresNo relevant conflicts of interest to declare.