Simple SummaryFish kept as pets are almost always held in tanks with substrate such as gravel or sand on the bottom of the tank. This may be added as a form of enrichment to encourage natural fish behaviours, or for aesthetic reasons. However, substrate can also harbour elevated levels of waste products and unwanted bacteria; therefore, whether the use of substrate in home aquaria is advantageous or disadvantageous has not been fully considered. Here, we investigated whether there was a difference in water quality in home aquaria that contained either no substrate (bare tanks), plastic plants as enrichment but no substrate, sand or gravel substrate. Water quality (e.g., temperature, oxygen, pH and ammonia) and the presence of bacteria were measured over a 7-week period. As water quality can also vary with the season, the study was repeated at different times of the year. Addition of both gravel and sand substrate resulted in increased pH and the waste products ammonia and nitrate. Substrate was also associated with a greater presence of bacteria. In conclusion, the use of substrate affected water quality, with further research needed on the use of substrate in home aquaria.Almost all home aquaria contain substrate, either as intentional enrichment or for aesthetic purposes. For fishes, benefits of structural enrichment have been well considered, particularly in research and aquaculture settings. However, our understanding of the impacts of tank substrate as enrichment is limited. While substrate can induce foraging in some species, a major drawback is the potential of substrate to harbour elevated levels of waste and pathogenic bacteria. Here, we considered whether substrate as a form of environmental enrichment significantly altered water quality and bacterial presence in home aquaria. Water quality (temperature, oxygen, pH, TAN, unionised ammonia, nitrate, Ca2+, Na+, Mg2+ and K+) and bacterial presence (Pseudomonas spp.) were measured over two seven-week periods in stand-alone, tropical, freshwater tanks that simulated home aquaria. The following four enrichment conditions were considered: bare tanks, plastic plants, gravel substrate or sand substrate. The addition of both gravel and sand resulted in increased pH, concentrations of total ammonia nitrogen and nitrate. Substrate was also associated with a greater Pseudomonas presence. Decreased pH alongside an increased concentration of ions were also observed depending on the time of year. In conclusion, enrichment type affected the water quality of home aquaria, with further research needed on the role of the tank biome in fish welfare.
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