Plastic Pieces Research Articles

Evaluation of Microplastics Ingested by Sea Cucumber Holothuria Scabra from Pulau Jambongan, Sabah.

Microplastics (MPs) are tiny plastic pieces having a diameter of less than 5mm. They can arise from larger plastic debris that degrades over time, synthetic fibres from clothing, microbeads in personal care items and even larger plastic debris. Sea cucumbers are marine creatures vital to the ocean's ecosystem as they assist in maintaining a clean seabed and recycle nutrients. The aim of this research was to characterize the types of MPs isolated from the sea cucumber Holothuria scabra from Pulau Jambongan, Sabah. A total of 30 H. scabra were collected. Their gastrointestinal tracts were removed and digested using NaOH. The digestates were filtrated several times to extract the MPs. MPs were observed under microscope and categorized into shapes and colours. To determine the functional group of polymers, further analysis using Fourier Transform-Infrared Spectroscopy (FTIR) was performed. The samples contained a total of 7403 MPs. Majority of MPs were black colored (72.25%) and in the form of fibres (99.05%). Two types of polymers were detected: polycarbonate (PC) found in 4% of the population and polymethyl methacrylate (PMMA) present in 96%. In conclusion, the presence of MPs in the gastrointestinal tract of H. scabra suggests that the animals and their habitat have been contaminated. Further study is required to comprehend the effects of MPs on sea cucumbers and other marine organisms as well as to develop prevention strategies.

Microplastic migration from landfill-mined soil through earth filling operations and ecological risk assessment: a case study in New Delhi, India.

Microplastics (MPs), plastic pieces smaller than 5 mm, are emerging as a critical ecological threat, potentially disrupting ecosystems and complicating waste management practices. Landfill-Mined Soil-Like Material (LMSLM), a byproduct of landfill reclamation, is gaining global traction for rehabilitating degraded land and repurposing it for geotechnical applications. While studies have examined contaminants like heavy metals and salts, MPs contamination has been largely overlooked, raising environmental concerns. The widespread use of LMSLM in earth-filling increases the risk of MP pollution. Additionally, significant gaps remain in understanding how MPs are distributed across different size fractions during sieving, which is critical for developing effective remediation strategies and informing future policies. This study aims to fill the existing knowledge gap by investigating the presence of microplastics in LMSLM collected from three aged waste dumpsites in New Delhi, India, and evaluating the ecological risks associated with its reuse. The results revealed the presence of MPs in all LMSLM samples with concentrations ranging from 25950 to 41110 items/kg. Fibers and fragments were the dominant shapes, with 60 % of particles measuring less than 425 µm. The color characteristics revealed the dominance of white, transparent, and black color. Polyethylene and polypropylene were the most common polymers, with smaller amounts of polyamide, polyethylene terephthalate, and polyester. SEM-EDX analysis revealed weathering effects and the presence of heavy metals, including Lead (Pb), Cadmium (Cd), and Arsenic (As), adsorbed on the MP surface. Ecological risk assessment using the Polymer Hazard Index and Pollution Load Index identified hazard levels of V and II, respectively, while the Potential Ecological Risk Index indicated a medium risk. Furthermore, the dimensional analysis demonstrates that MP width, particularly in fiber-shaped MPs, plays a crucial role in determining retention and migration during sieving, while MPs with uniform shapes, like spheres and fragments, exhibit limited movement. These findings underscore the need for protective measures when using LMSLM in geotechnical applications to prevent MP migration and contamination of surrounding environments. The study highlights the importance of further research on MP contamination in reclaimed landfill materials and its implications for sustainable land use and waste management.

Comparison of macrolitter and meso- and microplastic pollution on French riverbanks and coastal beaches using citizen science with schoolchildren.

Rivers are the major source of anthropogenic litter entering the ocean, especially plastic debris that accumulates in all ecosystems around the world and poses a risk to the biota. Reliable data on distribution, abundance, and types of stranded plastics are needed, especially on riverbanks that have received less attention than coastal beaches. Here, we present the citizen science initiative Plastique à la loupe (Plastic under the magnifier), which compares for the first time the distribution of different litter sizes (macrolitter and meso- and microplastics) over 81 riverbanks and 66 coastal beaches sampled in France between 2019 and 2021. A total of 147 school classes (3113 schoolchildren) from middle to high school collected, sorted, and enumerated 55,986 pieces of plastic to provide a baseline of the current pollution by stranded debris at the national level. Single-use plastics (mainly food-related items) were very abundant on riverbanks (43%), whereas fragmented debris dominated the macrolitter on coastal beaches (28%). Microplastics were always higher in number compared to mesoplastics and macrolitter, with polystyrene and polyethylene found in equivalent proportions on riverbanks while polyethylene dominated microplastics on coastal beaches. Tracing the source of plastic items was possible only for a small proportion of the numerous collected items, mainly for identifiable macrolitter and microplastic pellets. This study lays out the foundations for further works using the Plastique à la loupe citizen science initiative in France and additional comparisons to other studied habitats worldwide, which can be used by scientists and policy-makers for future litter monitoring, prevention and clean-up strategies.

An efficient extraction device for microplastics in marine sediments and its applications.

Microplastics, defined as small pieces of plastic with a size less than 5 millimeters, constitute a significant sink for microplastics in marine sediments. Given the potential harm to nature and human beings, accurate detection of microplastics in marine sediments is of the utmost importance. The separation of microplastics from marine sediments represents a pivotal step in the quantitative detection of microplastics. This paper presents a high-efficiency extraction device for microplastics in marine sediments, with the objective of enhancing the effectiveness and efficiency of microplastics extraction. The device employs an air pump to thoroughly mix the samples and incorporates metal perforated plate fillers to achieve efficient sedimentation, thus facilitating the separation of microplastics from the surrounding marine sediments. Subsequently, the separated microplastics are passed through a series of pore sizes of stainless steel screens and glass fibre filters via suction filtration, allowing for the collection of microplastics of varying particle sizes for subsequent identification. Following a series of method trials, the optimal extraction conditions for this device were identified. The results demonstrated its excellent extraction effectiveness and high efficiency. To verify the feasibility of this device, it was used to investigate the microplastics in the sediments of Dongzhai Harbor, Hainan. The abundance, particle size distribution, shape, and composition of microplastics in the sediments of this area were obtained, which not only validated the practicality of this microplastic extraction device but also provided significant insights for ecological and environmental protection in the region.

Additively manufactured plastic plasma spectrometer.

We report results in the development and testing of a low resource tophat electrostatic analyzer (ESA) for space plasma measurements. This device has been additively manufactured (3D-printed) using fused deposition modeling. The classic tophat design is composed of four plastic pieces, without any surface coatings. The three conducting electrodes are printed from carbon nanotube infused polyether ether ketone (CNT-PEEK). The fourth piece, an insulating electrode support, uses pure PEEK. This ESA is designed to detect electrons in space from 10eV up to 30keV. We demonstrate that the printed CNT-PEEK is sufficiently electrically conductive to support the fast high voltage slewing often required for high time resolution measurements. The plastic ESA has been successfully vibrated beyond standard pre-flight levels, tested under keV electron beam illumination over a wide range of temperatures, and tested under UV illumination, simulating the solar Ly-α flux. In comparison with an identical machined aluminum ESA, our CNT-PEEK ESA provides nominal energy/angle bandpasses, closely matching simulation. These bandpasses imply minimal impact from surface charging at beam energies of 2-3keV, although more investigation is needed. We also find that the CNT-PEEK ESA provides far superior out-of-band electron rejection and UV photon rejection compared to the machined aluminum ESA. We do not detect any problems with trapped gases or outgassing. This development offers the potential for significant mass savings, implementation of otherwise unattainable geometric configurations, and dramatic simplification in manufacturing and assembly processes required for the development of space plasma instruments.

Riparian vegetation entraps macroplastics along the entire river course: Implications for eco-safety activities and mitigation strategies

Macroplastic litter causes detrimental effects on freshwater biota affecting human health. Despite the significant role of rivers in transporting plastic waste, most plastics remain in fluvial ecosystems, accumulating in infrastructure, river sediment, and (riverbank) vegetated areas. However, the entrapment of plastics by riparian vegetation was overlooked, particularly in upper and middle river courses. For the first time, we aimed to quantify the entrapment of plastics by riparian vegetation along the entire river course. Sampling riparian areas in the upper, middle, and lower river courses in central Italy, we found 1548 macrolitter items, with vegetation entrapping 93.9% of total litter. Riverbank and riparian plastics acted as long-term indicators of river plastics. We emphasized the trapping efficiency at the species level highlighting that the best plastic trapper species were trees, shrubs and reeds (Populus spp., Salix spp., Rubus ulmifolius, Phragmites australis, and Ficus carica), blocking 85.4% of the total macrolitter entrapped by plants. Plastic pieces, bags, bandages, sanitary items, and packaging were among the most trapped types. Furthermore, vegetation in the lower river course exhibited greater plastic entrapment compared to the upper and middle courses, following the fact that all the river courses contribute to plastic pollution. Recognizing the potential of riparian vegetation as a valuable ecosystem service in trapping macroplastics, further research should explore the characteristics and structures of riparian communities involved in this process. By developing eco-safe practices and mitigation strategies based on these findings, we might contribute significantly to managing, conserving, and restoring riverine ecosystems.

Microplastics and Nanoplastics as Environmental Contaminants of Emerging Concern: Potential Hazards for Human Health

With nearly 40% of the total plastics produced being used for packaging, up to five trillion plastic bags are consumed in the world annually. The inadequate disposal of plastic waste and its persistence has become a serious challenge/risk to the environment, health, and well-being of living creatures, including humans. The natural degradation of plastics is extremely slow; large pieces of plastic may break down into microplastics (MPs) (1 μm–5 mm) or nanoplastics (NPs) (<1000 nm) after protracted physical, chemical, and/or biological degradations. A brief overview of the transport of micro- and nanoplastics in the aquatic, terrestrial, and atmospheric environments is presented. Details are provided on the exposure routes for these waste materials and their entry into humans and other biota through ingestion, inhalation, and dermal contact. The greatest concern is the cumulative impact of the heterogeneous secondary MPs and NPs on planetary and human health. Inhaled MPs and NPs have been shown to affect the upper respiratory tract, lower respiratory tract, and alveoli; prolonged exposure can lead to chronic inflammatory changes and systemic disease. These can also lead to autoimmune diseases and other chronic health conditions, including atherosclerosis and malignancy. Sustainable mitigation strategies to reduce the impact of MPs/NPs include source reduction, material substitution, filtration and purification, transformation of plastic waste into value-added materials, technological innovations, etc. Multidisciplinary collaborations across the fields of medicine, public health, environmental science, economics, and policy are required to help limit the detrimental effects of widespread MPs and NPs in the environment.

Microplastics induced ileum damage: Morphological and immunohistochemical study.

Microplastics (MPs) are small pieces of plastic that are widely distributed in the environment and accumulate within living organisms, so they are the most common types of pollutants at the present time. One of the most widespread types of MP in the environment is polyethylene (PE) MPs. There have been many published studies on the effect of PE MPs combined with other pollutants or chemicals such as benzoanthracene, emamectin benzoate, heavy metals and 4-nonylphenol, on some marine, amphibian, and mouse models. However, research has rarely been conducted on how single-use PE MPs affect the ileum of mammals. The current study is focused on the impact of PE MP exposure with different concentration (6, 60, 600 μg/mL PE/MPs) for 15 days, followed by 15 days of recovery on small intestine(ileum) of C57BL/6 murine model with precision and detail at the cell level by using different technique (histology, histochemistry, immunohistochemistry, and transmission electron microscope). Results demonstrated that the intestinal tissue exhibited nuclear pyknosis, villus deformation, shortness of villi, degeneration of lamina propria, hyperplasia of goblet cells, increase of goblet cells secretion, Alcian blue and Periodic acid-Schiff stain positivity of intact goblet cells, highly significance of P53 immunoreaction expression specially in high concentrations (600 μg/day of PE/MPs) and Ki-67 immunoreaction expression. RESEARCH HIGHLIGHTS: Different doses of microplastics (MPs) induced sever morphological alternations and clinical observations. MPs were deposits in cells and were observed in ultrastructure study. Recovery period able to ameliorate to the most extent the alternations caused by MPs administration.

Metagenomic analysis of soil from landfill site reveals a diverse microbial community involved in plastic degradation

In this study, we have investigated microbial communities structure and function using high throughput amplicon sequencing and whole metagenomic sequencing of DNA extracted from different depths of a plastic-laden landfill site. With diverse taxonomic groups inhabiting the plastic-rich soil, our study demonstrates the remarkable adaptability of microbes to use this new substrate as a carbon source. FTIR spectroscopic analysis of soil indicated degradation of plastic as perceived from the carbonyl index of 0.16, 0.72, and 0.44 at 0.6, 0.9 and 1.2 m depth, respectively. Similarly, water contact angles of 108.7 degree, 99.7 degree, 62.7 degree, and 77.8 degree of plastic pieces collected at 0.3, 0.6, 0.9, and 1.2 m depths respectively showed increased wettability and hydrophilicity of the plastic. Amplicon analysis of 16S and 18 S rRNA revealed a high abundance of several plastic-degrading bacterial groups, including Pseudomonas, Rhizobiales, Micrococcaceae, Chaetomium, Methylocaldum, Micromonosporaceae, Rhodothermaceae and fungi, including Trichoderma, Aspergillus, Candida at 0.9 m. The co-existence of specific microbial groups at different depths of landfill site indicates importance of bacterial and fungal interactions for plastic. Whole metagenome analysis of soil sample at 0.9 m depth revealed a high abundance of genes encoding enzymes that participate in the biodegradation of PVC, polyethylene, PET, and polyurethane. Curation of the pathways related to the degradation of these materials provided a blueprint for plastic biodegradation in this ecosystem. Altogether, our study has highlighted the importance of microbial cooperation for the biodegradation of pollutants. Our metagenome-based investigation supports the current perception that consortia of fungi-bacteria are preferable to axenic cultures for effective bioremediation of the environment.

Characteristics, Abundance and Polymer Type of Microplastics in Anadara granosa (Blood Clam) from Coastal Area of Palopo City

Plastic waste in marine waters will undergo a degradation process that breaks down large plastic pieces into smaller particles called microplastics. The abundance of microplastics, caused by their small size (<5mm) can be easily indirectly consumed by aquatic animals. Anadara granosa is one of the bivalves that is quite vulnerable to microplastic contamination because it has the nature of a filter feeder which means it can sift particles and organic matter around it. The purpose of this study was to determine the characteristics, abundance, and types of microplastic polymers in blood clams (A. granosa). The results of microplastic observations made on 60 blood clams were 153 microplastic particles identified from 47 individuals (78%) of contaminated blood clams with an average microplastic abundance of 0.591 ± 0.083 item/gr. Fiber-type microplastics are the most dominant form found and blue is the most dominant color found in the sample. Based on the average abundance of microplastics in Anadara granosa in the coastal area of Palopo City, it is lower than several studies that have been conducted previously. Fourier Transform-Infra Red was conducted to determine the type of polymer in microplastics. Three types of polymers were found in the Anadara granosa samples polyethylene terephthalate (PET), polystyrene, and polyester. The three types of polymers have effects on human health such as respiratory problems, skin irritation, and genotoxicity. Action is needed to prevent microplastic pollution in Palopo City’s rivers before microplastic pollution becomes more severe in the future.

Abundance of Marine Macrodebris on the Northern Coast of Jaffna Peninsula, Sri Lanka.

Marine plastic debris has emerged as a pressing concern along the northern coast of Jaffna, Sri Lanka, posing a significant threat to marine resources. A preliminary study was conducted to investigate the abundance and characteristics of marine plastic debris at four major fish landing sites in the northern coast of Jaffna, using the Clean Coast Index (CCI) and Plastic Abundance Index (PAI). The results revealed that the average abundance of marine debris and plastic debris were 1.71 ± 0.42 items/m2 and 1.66 ± 0.57 items/m2, respectively. The most common types of plastic debris represented plastic rope and net pieces (23.2%), followed by unidentified weathered plastic fragments (16.7%), beverage bottles (16.2%), bottle caps and lids (13%), and styrofoam (14.1%). The recognized sources of plastic debris were mainly fishing-based activities, recreation activities, transboundary sources, and unidentified sources. The fish landing sites were classified as exceptionally polluted, with a CCI exceeding 10 and a PAI value exceeding 8. Myliddy had the highest debris density, indicating substantial pollution levels, followed by Point Pedro, Mathagal, and Valveddithurai. These findings underscore the urgency of establishing a comprehensive plastic waste management framework for the northern coast of Jaffna and devising strategies to address buoyant debris within the northern Indian Ocean. Furthermore, this study carries significant implications for the local marine ecosystem, coastal communities, and Sri Lanka's broader environmental policies and practices.

Correlation between fishing yield and microplastic levels across nations

This research looks into the association between fishing and microplastic contamination across countries, with a particular focus on how the growth of microplastic levels can impact fishery production. Through the use of a mixed-effect model we were able to look through data from 100 nations during the period 1990-2021, where fishery yield data was extracted from The World Bank and microplastic concentration data was obtained from the National Centers for Environmental Information; population data served as a control variable in our analysis. We found that there is indeed a significant negative correlation between levels of microplastics and fishing yield: an increase by 1 piece of microplastic per cubic meter leads to decrease in fishing yield by anything between 65 and 100 metric tonnes (95% confidence interval). This relationship held true for about 93% of all coastal countries studied. To accommodate for differences amongst nations, we introduced random intercepts and slopes in our mixed-effect model which helped capture variations specific to each country while still identifying an overarching pattern. The research we are doing is on the connection between fish catches and microplastic pollution which takes place in the different countries of the world, where we focus more on how high microplastic levels influence fishery production. Having made use of a mixed-effect model, we have been able to look at data that represents 100 nations within the period of time between 1990 and 2021; The World Bank provided us with fishery yield data while microplastic concentration data came from the National Centers for Environmental Information. In addition to these variables, population data was used as a control variable. The summary of our analysis points towards a significant inverse relationship noted between microplastic levels and fishing yield: an increase by one piece of plastic results in a decline by somewhere between 65 to 100 metric tonnes (95% confidence interval). This generalization held true for about 93% coastal countries considered under this study. To capture specific variations among nations but also identify an overall trend line while dealing with inter-country variability, random intercepts and slope components were included as part of our mixed-effect model methodology.

Consumption of Bottled Water and Chronic Diseases: A Nationwide Cross-Sectional Study.

Plastic pollution is a growing concern. It can form smaller particles called microplastics (<5 mm). Microplastics can break down into even smaller pieces called nanoplastics (<1 μm). These minute particles can infiltrate human cells and tissues, with their health impacts still largely undetermined. On average, a liter of bottled water includes about 240,000 tiny pieces of plastic. The purpose of this study was to evaluate the association between the use of bottled plastic water (BW) and several health outcomes. Utilizing data from the Italian National Institute of Statistics' "Aspects of Daily Life" survey (N = 45,597), we employed logistic regression to explore the correlation between BW consumption and the prevalence of various chronic diseases, including hypertension, gastric/duodenal ulcers, and kidney stones. Adjustments were made for covariates such as education, age, gender, and economic resources. Our analysis indicated a statistically significant association between BW consumption and increased risk of hypertension (Odds ratio [OR] = 1.05, 95% confidence interval [CI] 1.00-1.11), diabetes (OR = 1.09, 95% CI 1.01-1.18), gastric/duodenal ulcers (OR = 1.21, 95% CI 1.07-1.38), and kidney stones (OR = 1.17, 95% CI 1.03-1.32). The consumption of BW is associated with heightened risk for certain health conditions. Policymakers and healthcare providers should consider implementing targeted prevention strategies and awareness campaigns.

Plastic ingestion, accumulated heavy metals, and health metrics of four Larus gull species feeding at a coastal landfill in eastern Canada

The objectives of this research were to assess ingested plastics and accumulated heavy metals in four urban gull species. Additionally, the relationships between ingested plastics and selected demographic and health metrics were assessed. Between 2020–2021 during the non-breeding seasons, 105 gulls (46 American herring gulls (HERG, Larus argentatus smithsonianus), 39 great black-backed gulls (GBBG, Larus marinus), 16 Iceland gulls (Larus glaucoides), 4 glaucous gulls (Larus hyperboreus)) were killed at a landfill in coastal Newfoundland and Labrador, Canada, as part of separate, permitted kill-to-scare operations related to aircraft safety. Birds were necropsied, the upper gastrointestinal tract contents were processed using standard techniques, and livers were analyzed for accumulated As, Cd, Hg, and Pb. The relationships between ingested plastics, demographics, and health metrics were assessed in HERG and GBBG. Across all four species, 85 % of birds had ingested at least one piece of anthropogenic debris, with 79 % ingesting at least one piece of plastic. We detected interspecific differences in plastic ingestion and hepatic trace metals, with increased ingested plastics detected in GBBG compared with HERG. For GBBG, levels of ingested plastic were relatively greater for birds with higher scaled mass index, while HERG with more ingested plastic had higher liver lead concentrations.

Investigating the Location and Types of Aspirated Foreign Bodies in the Airway of Hospitalized Patients

Objectives: Foreign body aspirations (FBAs) comprise the majority of accidental childhood deaths. The present study aimed to investigate the location and types of aspirated foreign bodies in hospitalized patients’ airways. Methods: In this cross-sectional study, the medical records of all consecutive patients with a primary diagnosis of FBA were reviewed retrospectively from 2019 to 2022. We extracted demographic profile (age and gender), type of aspirated foreign body, clinical manifestation at the time of presentation, clinical signs according to physical examination, and the time duration between presentation to hospital and removal of aspirated foreign body. Results: Of 101 patients, 65 were male, and most were under 4 years old. Nuts (53.5%) and organic bits (30.7%) were the most common aspirated foreign bodies. The bronchus (69.4%) was the most common location of aspirated foreign bodies. Most of the organic pieces (96.8%) and nuts (87%) were seen in the age group below 4 years old and also most of the cases of plastic pieces were related to children aged 4 to 18 years (P&lt;0.001). Cough and shortness of breath (68.3%) were the most common clinical complaints. Most patients with FBA in the trachea (65%) and bronchus (74%) had a normal clinical examination and a significant difference was observed between the clinical signs and location of the foreign body (P&lt;0.001). Fifty-four patients (53.5%) underwent foreign body intervention and removal less than 24 hours after aspiration. Discussion: A high frequency of FBA in children, especially in infants and toddlers, is due to starting feeding. It is very important to consider the differences in the types and locations of the aspirated object. Therefore, early rigid bronchoscopy is effective for inhaled foreign body removal with fewer complications.

Effects of biodegradable microplastics and straw addition on soil greenhouse gas emissions

Large pieces of plastic are transformed into microplastic particles through weathering, abrasion, and ultraviolet radiation, significantly impacting the soil ecosystem. However, studies on biodegradable microplastics replacing traditional microplastics as agricultural mulching films to drive the biogeochemical processes influenced by GHG are still in their initial stages, with limited relevant reports available. This study sought to investigate the effects of microplastic and straw addition on CO2 and N2O emissions in different soils. Herein, yellow-brown soil (S1) and fluvo-aquic soil (S2) were utilized, each treated with three different concentrations of PLA (polylactic acid) microplastics (0.25%, 2%, and 7% w/w) at 25 °C for 35 days, with and without straw addition. The results showed that straw (1% w/w) significantly increased soil CO2 by 4.1-fold and 3.2-fold, respectively, and N2O by 1.8-fold and 1.8-fold, respectively, in cumulative emissions in S1 and S2 compared with the control. PLA microplastics significantly increased CO2 emissions by 71.5% and 99.0% and decreased N2O emissions by 30.1% and 24.7% at a high concentration (7% w/w, PLA3) in S1 and S2 compared with the control, respectively. The same trend was observed with the addition of straw and microplastics together. Structural equation modeling and redundancy analysis confirmed that soil physiochemical parameters, enzyme and microbial activities are key factors regulating CO2 and N2O emissions. The addition of microplastics is equivalent to the addition of carbon sources, which can significantly affect DOC, MBC, SOC and the abundance of carbon-associated bacteria (CbbL), thereby increasing soil CO2 emissions. The addition of microplastics alone inhibited the activity of nitrogen cycling enzymes (urease activity), increasing the abundance of denitrifying microbes. However, adding a high amount of microplastics and straw together released plastic additives, inhibiting microbial abundance and reducing the nitrogen cycle. These effects decreased NH4+-N and increased NO3−-N, resulting in decreased N2O emissions. This study indicates that biodegradable microplastics could reduce soil plastic residue pollution through degradation. However, their use could also increase CO2 emissions and decrease N2O emissions. Consequently, this research lays the groundwork for further investigation into the implications of utilizing biodegradable microplastics as agricultural mulch, particularly concerning soil geochemistry and GHG emissions.

Hunting for pigments in bacterial settlers of the Great Pacific Garbage Patch.

The Great Pacific Garbage Patch, a significant collection of plastic introduced by human activities, provides an ideal environment to study bacterial lifestyles on plastic substrates. We proposed that bacteria colonizing the floating plastic debris would develop strategies to deal with the ultraviolet-exposed substrate, such as the production of antioxidant pigments. We observed a variety of pigmentation in 67 strains that were directly cultivated from plastic pieces sampled from the Garbage Patch. The genomic analysis of four representative strains, each distinct in taxonomy, revealed multiple pathways for carotenoid production. These pathways include those that produce less common carotenoids and a cluster of photosynthetic genes. This cluster appears to originate from a potentially new species of the Rhodobacteraceae family. This represents the first report of an aerobic anoxygenic photoheterotrophic bacterium from plastic biofilms. Spectral analysis showed that the bacteria actively produce carotenoids, such as beta-carotene and beta-cryptoxanthin, and bacteriochlorophyll a. Furthermore, we discovered that the genetic ability to synthesize carotenoids is more common in plastic biofilms than in the surrounding water communities. Our findings suggest that plastic biofilms could be an overlooked source of bacteria-produced carotenoids, including rare forms. It also suggests that photoreactive molecules might play a crucial role in bacterial biofilm communities in surface water.

Marine litter along the shores of the Persian Gulf, Iran

Plastic wastes —including cigarette butts (CBs)— are dangerous for marine ecosystems not only because they contain hazardous chemicals but also because they can finally turn into micro- or even nano-particles that may be ingested by micro- and macro-fauna. Even large pieces of plastics can trap animals. In this research, the pollution status of macroplastics (abundance, size, type, and colour) and cigarette butts (CBs, number/m2) on the northern coasts of the Persian Gulf has been investigated. A total of 19 stations were explored in Bushehr province (Iran), which covers a length equivalent to 160 km of the Persian Gulf coastline. Among the collected plastic waste (2992 items), disposable mugs were the most frequent (18 %). Plastics with sizes 5–15 cm were the most abundant, and the most common type of plastic was PET (P-value <0.05). The origin of most macroplastics was domestic (2269 items). According to the Index of Clean Coasts (ICC), most surveyed beaches were extremely dirty. The average number and density of CBs in this study were 220 and 2.45 items/m2, respectively. Household litter was the most abundant type of waste in the studied beaches, and this problem can be better managed by training and improving the waste disposal culture. In general, it is suggested that an integrated and enhanced management for fishing, sewage and surface water disposal, and sandy recreational beaches be implemented in Bushehr to control plastic waste.

Message in a (Plastic) Bottle

Microplastics are plastic pieces less than 5 mm in diameter and are a widespread contaminant in waterways worldwide. Microplastics can be split into four categories: beads, fragments, thin films, or fibers. In this activity, students learn about microplastics in the environment and as hazards to marine life. Students create a “MicroGlobe” that mimics real-world microplastic contamination in marine environments. Students calculate the percentages of different types of microplastics and generate pie charts of the collected data. They are challenged to think about ways they can be better stewards of the Earth and their answers in the math exercise are used to answer questions about how their “MicroGlobes” differ from real-world data in the provided Activity Packet. This activity has modifications for students with visual impairments.

On the use of anthropogenic materials in nest building of House Wren (Troglodytes aedon), a report from Parque Los Algarrobos, Cumbayá, Ecuador

Urbanization has prompted shifts in avian nesting behaviors, with some bird species incorporating anthropogenic nest materials (ANMs) into their nests. This study focuses on Troglodytes aedon (House Wren) in Ecuador, exploring the factors influencing their use of ANMs. Observations in Parque Los Algarrobos revealed a House Wren carrying a piece of transparent plastic, entering a street lamp and emergind from it without the plastic. Additional data from iNaturalist showcased widespread ANM use in House Wren nests across Ecuador.