Plasma-generated Reactive Species Research Articles

Human coronavirus 229E inactivation on porous and nonporous materials using dielectric barrier discharge (DBD) plasma.

Viruses can spread through contaminated aerosols and contaminated surface materials, and effective disinfection techniques are essential for virus inactivation. Nonthermal plasma-generated reactive oxygen and nitrogen species can effectively inactivate the coronavirus. We aim to interpret the coronavirus inactivation level and mechanism of surface interaction with materials with and without dielectric barrier discharge (DBD) plasma treatment. Nonthermal plasma, particularly surface-type DBD plasma, can inactivate human coronavirus 229E (HCoV-229E) on porous (paper, wood, mask) and nonporous (plastic, stainless steel, glass, Cu) materials. Virus inactivation was analyzed using a 50% tissue culture infectivity dose (TCID50) using cell line, flow cytometry, and immunofluorescence. Surfaces contaminated with HCoV-229E were treated at different time intervals (0-5 h) with and without plasma exposure (natural decay in ambient air conditions). HCoV-229E persistence conformed to the following order: plastic > cover glass > stainless steel > mask > wood > paper > Cu with and without plasma exposure. HCoV-229E was more stable in plastic, cover glass, and stainless steel in 5 h, and the viable virus titer gradually decreased from its initial log10 order of 6.892 to 1.72, 1.53, and 1.32 TCID50/mL, respectively, under plasma exposure. No virus was observed in Cu after treatment for 5 h. The use of airflow, ambient nitrogen, and argon did not promote virus inactivation. Flow cytometry and immunofluorescence analysis demonstrated a low expression level of spike protein (fluorescence intensity) during plasma treatment and in E and M genes expression compared with the virus control.

Treatment of seeds by cold ambient air plasma: combining impedance measurements with water sorption modeling to understand the impact of seed hydration

In this article, we focus on the plasma seed interaction and more specifically-on the feedback exerted by the seeds on the plasma properties. Dormant Arabidopsis seeds with different water contents (WC), namely 3%DW, 10%DW and 30%DW were exposed to cold ambient air plasma (C2AP) generated in a dielectric barrier device (DBD). It is found that increasing WC enhances the capacitive current of the DBD, generates a greater number of low energy streamers (characterized by current peaks lower than 10 mA) that preferentially interplay with the seeds. Since the resistive and capacitive components of the seeds modify the C2AP electrical properties, impedance measurements (also called LCRmetry) have been carried out to measure their main dielectric parameters before/after plasma exposure (seeds resistance, capacitance, complex relative permittivity, tangent loss and conductivity). It appears that WC significantly changes dielectric losses at low frequencies (<1 kHz) due to polarization relaxation of the polar molecules (i.e. water). LCRmetry further reveals that C2AP does not substantially alter seeds dielectric parameters, i.e. it neither adds or removes significant amounts of new materials, meaning that the relative starch, protein and lipid contents remain essentially unaffected. However, it cannot be discounted that some bulk properties of the Arabidopsis seeds may be modified, especially regarding their porosity. This characteristic could facilitate penetration of plasma-generated reactive oxygen species into the internal seed tissues, leading to the grafting of oxygenated groups. To corroborate this theory, water sorption isotherms have been achieved on Arabidopsis seeds and fitted with four thermodynamic models, including the Brunauer–Emmett–Teller model and the Generalized D’Arcy and Watt model. It is demonstrated that C2AP primarily strengthens water-seed affinity by modifying molecular interactions rather than changing the seed’s moisture layer. This occurs despite a potential decrease in the number of adsorption sites, indicating a significant increase in overall seed hydrophilicity after plasma treatment.

Non-thermal atmospheric-pressure positive pulsating corona discharge in degradation of textile dye Reactive Blue 19 enhanced by Bi2O3 catalyst

In this work, monoclinic Bi2O3 was applied for the first time, to the best of our knowledge, as a catalyst in the process of dye degradation by a non-thermal atmospheric-pressure positive pulsating corona discharge. The research focused on the interaction of the plasma-generated species and the catalyst, as well as the role of the catalyst in the degradation process. Plasma decomposition of the anthraquinone reactive dye Reactive Blue 19 (RB 19) was performed in a self-made reactor system. Bi2O3 was prepared by electrodeposition followed by thermal treatment, and characterized by x-ray diffraction, scanning electron microscopy and energy-dispersive x-ray techniques. It was observed that the catalyst promoted decomposition of plasma-generated H2O2 into •OH radicals, the principal dye-degrading reagent, which further attacked the dye molecules. The catalyst improved the decolorization rate by 2.5 times, the energy yield by 93.4% and total organic carbon removal by 7.1%. Excitation of the catalyst mostly occurred through strikes by plasma-generated reactive ions and radical species from the air, accelerated by the electric field, as well as by fast electrons with an energy of up to 15 eV generated by the streamers reaching the liquid surface. These strikes transferred the energy to the catalyst and created the electrons and holes, which further reacted with H2O2 and water, producing •OH radicals. This was indentified as the primary role of the catalyst in this process. Decolorization reactions followed pseudo first-order kinetics. Production of H2O2 and the dye degradation rate increased with increase in the input voltage. The optimal catalyst dose was 500 mg∙dm−3. The decolorization rate was a little lower in river water compared with that in deionized water due to the side reactions of •OH radicals with organic matter and inorganic ions dissolved in the river water.

Coupling the COST reference plasma jet to a microfluidic device: a computational study

The use of microfluidic devices in the field of plasma-liquid interaction can unlock unique possibilities to investigate the effects of plasma-generated reactive species for environmental and biomedical applications. So far, very little simulation work has been performed on microfluidic devices in contact with a plasma source. We report on the modelling and computational simulation of physical and chemical processes taking place in a novel plasma-microfluidic platform. The main production and transport pathways of reactive species both in plasma and liquid are modelled by a novel modelling approach that combines 0D chemical kinetics and 2D transport mechanisms. This combined approach, applicable to systems where the transport of chemical species occurs in unidirectional flows at high Péclet numbers, decreases calculation times considerably compared to regular 2D simulations. It takes advantage of the low computational time of the 0D reaction models while providing spatial information through multiple plug-flow simulations to yield a quasi-2D model. The gas and liquid flow profiles are simulated entirely in 2D, together with the chemical reactions and transport of key chemical species. The model correctly predicts increased transport of hydrogen peroxide into the liquid when the microfluidic opening is placed inside the plasma effluent region, as opposed to inside the plasma region itself. Furthermore, the modelled hydrogen peroxide production and transport in the microfluidic liquid differs by less than 50% compared with experimental results. To explain this discrepancy, the limits of the 0D–2D combined approach are discussed.

The potential of multicylindrical dielectric barrier discharge plasma for diesel-contaminated soil remediation and biocompatibility assessment

This study explored the use of multicylindrical dielectric barrier discharge (MC–DBD) plasma technology to eliminate diesel fuel contamination from the soil. This study also assessed the environmental impact of plasma-generated reactive species on soil properties, plant growth, and the safety of microbial and human skin cells using various analytical methods. MC–DBD plasma was characterized using the current–voltage analysis and optical emission spectroscopy (OES). Gas Fourier transform infrared spectroscopy was employed to detect reactive species, such as O3, NO, NO2, N2O, and HNO3, in the plasma-treated air. The diesel fuel concentration in the soil was measured before and after plasma treatment using a gas chromatography–flame ionization detector. The efficacy of the MC–DBD plasma treatment was evaluated based on soil characteristics (pH and moisture), discharge parameters (power), and reactive species (O3 and NOx). Using only power of 30 W, the MC–DBD achieved a 94.19% removal of diesel fuel from the soil and yielded an energy efficiency of 1.78 × 10−2 m3/kWh within a 60-min treatment period. Neutral soil with a moisture content of 2% proved more effective in diesel fuel removal compared with acidic or alkaline soil with higher moisture content. O3 was the most efficient plasma-generated reactive species for diesel fuel removal and is involved in oxidation-induced fragmentation and volatilization. Overall, the potential of the MC–DBD plasma technology for remediating diesel fuel-contaminated soils is highlighted, and valuable insights for future applications are provided.

Highlight the plasma-generated reactive oxygen species（ROSs）dominant to degradation of emerging contaminants based on experiment and density functional theory

This paper aims to deeply analyze the degradation mechanism of emerging contaminants by dielectric barrier discharge (DBD) plasma through experiments and simulations. It was confirmed that DBD plasma exhibits a high degradation effect on three emerging contaminants by the generated reactive oxygen species (ROSs). Increasing the output power leads to higher ROSs production and higher degradation rates of emerging contaminants. At an input power of 94.6 W, the degradation rates of sulfamethoxazole (SMX), bisphenol A (BPA) and ciprofloxacin (CIP) reach 94.5 %, 92.9 %, and 98.6 %, respectively. Trapping agent experiments were conducted to investigate the role of ROSs (·OH, ·O2–, and 1O2) in emerging contaminants degradation. Notably, capturing of 1O2 led to a decrease of up to 31 % in the degradation rate of BPA. Electron spin resonance (ESR) tests confirmed the involvement of various ROSs, especially 1O2, in the degradation process. Density functional theory (DFT) was employed to further explore the action mechanism of ROSs in the DBD plasma system. For SMX and BPA, the degradation is initiated by the dissociation of annular structures, while for CIP, annular structures are directly opened by ROSs. These findings highlight the importance of 1O2 and ·OH in the degradation process. Finally, the toxicity of intermediates produced by ROSs-dominate to degradation was declined. This work can provide new insights and unique guidance on the plasma degradation mechanism of emerging contaminants.

Persistence of Coronavirus on Surface Materials and Its Control Measures Using Nonthermal Plasma and Other Agents.

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been responsible for the initiation of the global pandemic since 2020. The virus spreads through contaminated air particles, fomite, and surface-contaminated porous (i.e., paper, wood, and masks) and non-porous (i.e., plastic, stainless steel, and glass) materials. The persistence of viruses on materials depends on porosity, adsorption, evaporation, isoelectric point, and environmental conditions, such as temperature, pH, and relative humidity. Disinfection techniques are crucial for preventing viral contamination on animated and inanimate surfaces. Currently, there are few effective methodologies for preventing SARS-CoV-2 and other coronaviruses without any side effects. Before infection can occur, measures must be taken to prevent the persistence of the coronavirus on the surfaces of both porous and non-porous inanimate materials. This review focuses on coronavirus persistence in surface materials (inanimate) and control measures. Viruses are inactivated through chemical and physical methods; the chemical methods particularly include alcohol, chlorine, and peroxide, whereas temperature, pH, humidity, ultraviolet irradiation (UV), gamma radiation, X-rays, ozone, and non-thermal, plasma-generated reactive oxygen and nitrogen species (RONS) are physical methods.

Plasma–liquid interactions: an experiment and simulation study on plasma dynamic behaviors near the gas–liquid interfacial layer

Plasma–liquid interaction processes are essential to various plasma applications such as sewage disposal, biomedicine, and synthesis of nanomaterials. However, the near gas–liquid interfacial behavior of plasma property remains inadequately understood, hindering the controllability of the application process. This study combines experimental diagnostics and simulations to investigate the production and transport of plasma-generated reactive species near (∼35 μm) the gas–liquid interfacial layer. The experimental results are used to benchmark densities obtained from a zero-dimensional plasma chemical kinetics simulation, which reveals the time evolutions of plasma-generated active species. A large number of neutral particles (like OH, H2O2) and water cluster ions (like H9O4 +) were produced as a result of the evaporation effect of the cathode solution surface. The estimation of energy flux from the gaseous plasma to liquid surface showed that the hydration process of positive ions plays the key role in the resulted water evaporation at the gas–liquid interfacial layer.

Global numerical simulation of chemical reactions in phosphate-buffered saline (PBS) exposed to atmospheric-pressure plasmas

Abstract When a phosphate-buffered saline (PBS) solution is exposed to atmospheric-pressure plasmas generated in air, hydrogen peroxide H 2 O 2 in the solution is known to be decomposed by hypochlorite O C l − , which is formed in the solution from reactions between chlorine ions C l − present in the PBS solution and plasma-generated reactive oxygen species. Global numerical simulations of liquid-phase chemical reactions were performed to identify the reaction mechanisms of H 2 O 2 decomposition by solving known liquid-phase chemical reactions self-consistently. It has been confirmed that the decomposition of H 2 O 2 is indeed mostly due to the presence of O C l − in the solution. However, this study has also found that, in the presence of abundant hydroxyl ( O H ) radicals, the most efficient H 2 O 2 decomposition pathway can be a series of reactions that we call a chlorine monoxide cycle, where O C l − first reacts with O H to generate chlorine monoxide C l O , which then decomposes H O C l , rather than O C l − directly decomposing H 2 O 2 . The chlorine monoxide cycle generates O H as one of its byproducts, so once this cycle is initiated, it continues until either C l O − or H 2 O 2 runs out, as long as none of the intermediates are scavenged by other reactions.

Mechanistic study on the synergy of cold plasma and sulfate radical in the degradation of azo and triarylmethane dyes using density functional theory

The activation of persulfate using cold plasma and the synergy of plasma-generated reactive species and SO4•− in mineralization of organic dyes were assessed. In this regard, solutions of crystal violet (CV) as a triarylmethane dye and methyl orange (MO) as an azo dye, were treated with cold plasma, UV-C-activated sulfate radicals, and their combination. The achieved results showed 95% TOC removal, a synergy factor of 1.3 and an energy yield of 1.68 (g kWh−1) for the combined system. The contribution of oxidant species in CV (SO4•− > O2•- > •OH) and MO (SO4•−> O2•- >1O2) degradation was explored. Moreover, analysis of degradation products and density functional theory (DFT) calculations were adopted to find the degradation mechanism based on the minimum energy pathway. Experimental and DFT results revealed that while radical and nucleophilic pathways were the drivers of CV degradation, the electrophilic attack of oxidants was a more plausible mechanism for MO degradation. Additionally, a comparison of the results of different DFT methods with experimental values showed that the Kohn–Sham DFT yielded more accurate results compared to recommended double hybrid methods. Analysis of a treated real wastewater revealed that the background constituents had negligible effects on MO and CV degradation. The high TOC removal efficiency of the combined system suggests its feasibility for treating wastewater from fertilizer and pharmaceutical production facilities.

Coupling the COST reference plasma jet to a microfluidic device: a new diagnostic tool for plasma-liquid interactions

Plasma-liquid interaction processes are central to plasma applications in medicine, environment, and material processing. However, a standardized platform that allows the study of the production and transport of plasma-generated reactive species from the plasma to the liquid is lacking. We hypothesize that use of microfluidic devices would unlock many possibilities to investigate the transport of reactive species in plasma-treated liquids and, ultimately, to measure the effects of these species on biological systems, as microfluidics has already provided multiple solutions in medical treatment investigations. Our approach combines a capacitively coupled RF plasma jet known as the COST reference plasma jet with simple 3D printed microfluidic devices. This novel pairing is achieved by carefully controlling capillary effects within the microfluidic device at the plasma-liquid interaction zone. The generation and transport of reactive species from the plasma to the liquid inside the microfluidic device are analyzed using a colorimetric hydrogen peroxide concentration assay. A capillary flow model is provided to explain the two main regimes of operations observed in the device and their merits are discussed. Overall, the proposed plasma-microfluidic prototype shows great potential for the fundamental study of plasma-liquid interactions and opens the way to the use of standard microfluidic devices with plasma sources developing a plasma column or a plasma plume.

Influence of a transient spark plasma discharge on producing high molecular masses of chemical products from l-cysteine

Cold atmospheric pressure plasmas are considered a forthcoming method in many research areas. Plasma modification of biomolecules has received much attention in addition to plasma-treated biomaterials. Hence, in this work, we operated a transient spark plasma (TSP) discharge to study its effect on the l-cysteine chemical structure. the TSP was configured in a pin-to-ring electrode arrangement and flowed by Ar gas. We also investigated the effect of two chemicals; dimethyl sulfoxide (DMSO) and hydrogen peroxide (H2O2) by the bubbling method to show how they can change the creation of new chemical bioproducts. Ultraviolet–Visible absorption spectroscopy, Fourier transform infrared spectroscopy and Liquid chromatography–mass spectroscopy were used to investigate any changes in chemical bonds of cysteine structure and to depict the generation of new biomolecules. Based on the displayed results plasma-generated reactive species had a great role in the chemical structure of the cysteine. Entering DMSO and H2O2 into the plasma caused the creation of new products and the heaviest biomolecule was produced by the simultaneous addition of DMSO and H2O2. The results also predicted that some chemical products and amino acids with a higher value molecular masse produced from the polymerization process of cysteine solution. The strong oxidation process is responsible for the heavy chemical compounds.

Power dependence of reactive species generation in a water falling film dielectric barrier discharge system

The short-lived and long-lived reactive species generated in plasma-liquid interactions determine their potential applications in wastewater purification and plant growth stimulation. In this work, plasma-generated reactive species including hydroxyl radicals (∙OH), hydrogen peroxide (H2O2), ozone (O3), hydronium (H3O+), and nitrate (NO3-) were quantified in a water falling film dielectric barrier discharge system. The reaction kinetics of short-lived ∙OH were analyzed based on the quantification of ∙OH and degradation of salicylic acid, focusing on the contribution and utilization of ∙OH. The interconversion of ∙OH with H2O2 and O3 was also calculated. The accumulation rates of H3O+ and NO3- indicated the potential of this dielectric barrier discharge system in sustainable fertilizer production. In addition, the toxicity prediction of salicylic acid degradation intermediates and the cultivation of soybean seeds verified the feasibility of plasma-liquid interactions in organic wastewater recycling.

Plasma-enhanced microbial electrolytic disinfection: Decoupling electro- and plasma-chemistry in plasma-electrolyzed oxidizing water using ion-exchange membranes

Pathogenic microorganisms pose a global threat to public health and environment. Common antibacterial chemicals produce toxic residues, inevitably harming the environment. Electrolyzed oxidizing water (EOW), a promising environment-friendly alternative disinfectant, still lacks effective production processes, sufficient bactericidal efficacy and stability, while the enabling physico-chemical mechanisms remain unclear. Here, we report, for the first time, an effective hybrid plasma electrochemical EOW production process and reveal the mechanisms by combining nonthermal plasmas and a two-chamber electrochemical cell separated by a cation exchange membrane (CEM) for decoupling the chemical reactions during the plasma treatment of water. Experimental results demonstrate that combined chlorine (chloramine) was the main chlorine product in the plasma-enhanced EOW (P-EOW) without a membrane, owing to the consumption of free chlorine (Cl2, HOCl, ClO−) by plasma-generated reactive nitrogen species. With a CEM in the plasma electrolysis system and through controlling the plasma discharge polarity, the production of free chlorine and other reactive species can be selectively controlled, with the highest concentration of free chlorine obtained in the negative plasma-enhanced EOW (NP-EOW). According to the transportation of cations by the CEM, the high concentrations of free chlorine may be attributed to the higher consuptions of H+ in cathode cell of negative plasma. The study of antibacterial ability of EOW produced under different conditions revealed that Staphylococcus aureus cells were best inactivated by the NP-EOW with CEM, which is mainly attributed to the higher concentration of free chlorine. This study demonstrates the feasibility of plasma-enhanced microbial electrolytic disinfection and offers new insights into the fundamental aspects of P-EOW chemistries for the future development of sustainable, efficient, and cost-effective multipurpose sustainable chemical technologies for water research and treatment.

Plasma–induced rapid crystallization and surface engraving of amorphous TiOx(OH)y to enhance adsorption and photocatalytic activity

Herein, we highlight a facile, green, and controlled technique commonly known as plasma-liquid interaction for crystal growth, defect engineering, and surface modification of amorphous titanium oxide. Using a novel two-step procedure combining hydrolysis and plasma treatment, we have fabricated defect-enriched anatase TiO2 powder. Rapid crystallization of amorphous titanium oxide is observed due to the interaction of plasma-generated reactive species with the solvated material. However, prolonged treatment time decreases the material's crystallinity due to excess generation of oxygen vacancy defects (OVD) in the TiO2 lattice. The plasma treatment also engraves the material’s surface forming the porous structure, increasing the specific surface area to ∼46 %. At a higher treatment time, TiO2 nanoparticles are grown on the material’s surface. The dye adsorption of the material is quite rapid, and the performance depends on the material’s surface condition. This rapid adsorption speeds up the photo-degradation performance (up to ∼96 %) of the material. The presence of OVD increases the photocatalytic activity of the material. However, an excess amount of OVD quenches the activity. A comparative study between ambient air and argon plasma-assisted material modification for optimized adsorption and photocatalytic performance has been carried out.

Ultrasound-Assisted High-Voltage Cold Atmospheric Plasma Treatment on the Inactivation and Structure of Lysozyme: Effect of Treatment Voltage

Emerging nonthermal processing techniques are gaining much attention due to their numerous advantages over traditional methods. Thus, in this study, high-voltage cold atmospheric plasma (HV-CAP) was employed separately and synergetically with ultrasound on lysozyme to investigate its activity and structure. The HV-CAP was operated at 120, 140, and 160 kV for 3 min at a frequency of 120 Hz. A significant difference was observed between the untreated and both treatments in lysozyme activity, with a more pronounced result in the ultrasound-assisted HV-CAP treatment. Both treatments resulted in the modification of the tertiary structure of the lysozyme as lipid oxidation, carbonyl content, UV spectra, intrinsic fluorescence, hydrophobicity, and sulphhydryl groups increased with increased voltage. Particle size distribution of lysozyme shows that noncovalent interactions affected the tertiary structure. The effects of both treatments on the secondary structure of lysozyme were demonstrated by CD and relative molar ellipticity. The results indicated that plasma-generated reactive species and ultrasound-induced cavitation influenced the unfolding of tertiary and secondary structures of the lysozyme. The CD spectra postulated that lysozyme inactivation was closely linked to the α-helix and β-sheet components of the secondary structure. This study presents an original novel knowledge on the modification of activity and structure of lysozyme subjected to ultrasound-assisted HV-CAP treatment that has been missing from the literature.Graphical abstract

Molecular mechanisms of seed dormancy release by gas plasma-activated water technology.

Developing innovative agri-technologies is essential for the sustainable intensification of global food production. Seed dormancy is an adaptive trait which defines the environmental conditions in which the seed is able to germinate. Dormancy release requires sensing and integration of multiple environmental signals, a complex process which may be mimicked by seed treatment technologies. Here, we reveal molecular mechanisms by which non-thermal (cold) atmospheric gas plasma-activated water (GPAW) releases the physiological seed dormancy of Arabidopsis thaliana. GPAW triggered dormancy release by synergistic interaction between plasma-generated reactive chemical species (NO3–, H2O2, ·NO, and ·OH) and multiple signalling pathways targeting gibberellin and abscisic acid (ABA) metabolism and the expression of downstream cell wall-remodelling genes. Direct chemical action of GPAW on cell walls resulted in premature biomechanical endosperm weakening. The germination responses of dormancy signalling (nlp8, prt6, and dog1) and ABA metabolism (cyp707a2) mutants varied with GPAW composition. GPAW removes seed dormancy blocks by triggering multiple molecular signalling pathways combined with direct chemical tissue weakening to permit seed germination. Gas plasma technologies therefore improve seed quality by mimicking permissive environments in which sensing and integration of multiple signals lead to dormancy release and germination.

Digitally manufactured air plasma-on-water reactor for nitrate production

The sustainable production of food to support the increasing world population is one of humanity’s most pressing challenges. Plasma activated water, produced using renewable energy, can help fulfill plants’ needs in sustainable agriculture approaches. The design, implementation, and characterization of a digitally manufactured air plasma-on-water reactor (POWR) for the synthesis of nitrate as green nitrogen fertilizer is presented. The interaction of air plasma-generated reactive oxygen and nitrogen species with water produces nitrate (NO3 −) and related species, which are the main nitrogen-containing nutrients for plants. The mild conditions of the operation of the POWR opens the possibility to use plastics, particularly through digital manufacturing strategies such as 3D-printing, for its fabrication. A pin-to-plate reactor configuration powered by high-voltage alternating power is chosen due to its simplicity and efficacy. A computational thermal-fluid model is used to evaluate the design and attain expected operational characteristics. The experimental characterization of the POWR encompassed design and operation parameters, namely electrode-water spacing, air flow rate, and voltage level. A machine learning approach is implemented to extract and quantify characteristic features of the plasma–water interaction, such plasma volume and plasma–water interface area. Experimental results revealed that the nitrate production rate varies linearly with dimensionless plasma volume. The design, fabrication, and characterization methods presented can be adapted to other POWRs and help enable on-demand nitrogen fertilizer production at low environmental and economic cost.

Non-thermal plasmas for disease control and abiotic stress management in plants

Climate change is predicted to cause severe loss in agricultural production by increasing disease epidemics and intensifying abiotic stresses. Therefore, there is a need for sustainable methods to alleviate plant stress, such as non-thermal plasma. Here we review the role of non-thermal plasma for plant treatment, with focus on the control of viruses, bacteria, fungi and other diseases. We present factors influencing the microbicidal activity of non-thermal plasma. Application of non-thermal plasma for combating abiotic stresses such as drought, metal toxicity, nanoparticles and salinity are discussed. Plasma-generated reactive species trigger the activity of stress-responsive genes in plants. The hypothetical mechanisms involved in triggering the activity of different stress-responsive genes controlling diseases as well as abiotic stresses, are also presented and discussed. The mechanism of plant-plasma interaction is similar to priming, hormesis or adaptive response, and resembles vaccination in animals and humans.

Nitrate Capture Investigation in Plasma-Activated Water and Its Antifungal Effect on Cryptococcus pseudolongus Cells.

This research investigated the capture of nitrate by magnesium ions in plasma-activated water (PAW) and its antifungal effect on the cell viability of the newly emerged mushroom pathogen Cryptococcus pseudolongus. Optical emission spectra of the plasma jet exhibited several emission bands attributable to plasma-generated reactive oxygen and nitrogen species. The plasma was injected directly into deionized water (DW) with and without an immersed magnesium block. Plasma treatment of DW produced acidic PAW. However, plasma-activated magnesium water (PA-Mg-W) tended to be neutralized due to the reduction in plasma-generated hydrogen ions by electrons released from the zero-valent magnesium. Optical absorption and Raman spectra confirmed that nitrate ions were the dominant reactive species in the PAW and PA-Mg-W. Nitrate had a concentration-dependent antifungal effect on the tested fungal cells. We observed that the free nitrate content could be controlled to be lower in the PA-Mg-W than in the PAW due to the formation of nitrate salts by the magnesium ions. Although both the PAW and PA-Mg-W had antifungal effects on C. pseudolongus, their effectiveness differed, with cell viability higher in the PA-Mg-W than in the PAW. This study demonstrates that the antifungal effect of PAW could be manipulated using nitrate capture. The wide use of plasma therapy for problematic fungus control is challenging because fungi have rigid cell wall structures in different fungal groups.