Plasma Coating Research Articles

Abstract A013: Quality control of the biobanked matched tissue: Blood samples for the development of diverse liquid based molecular assays

Abstract The availability of high-quality matched biological samples (tumor-blood) is the major limiting factor for research and development of novel, liquid biopsy-based assays in oncology. Frequently, ongoing specifically timed collections of blood samples are required. De-identified tissue blocks (formalin-fixed, paraffin embedded (FFPE) or flash frozen) matched with patient's blood samples collected in fixative-containing (Streck) of EDTA containing tubes, were collected at worldwide geographic locations and transferred to biobanking facility (Reference Medicine, Phoenix, AZ). Additional blood samples were collected at various times regarding the cancer surgery. Plasma and buffy coat samples were locally prepared and shipped to the biobank. A subset of blood samples without matched tissues from healthy donors were also collected. All samples were shipped under strict transport conditions. Quality control performed at the biobanking facility included pathology review and nucleic acids quality assessment. A total of over 3,000 cases were collected and provided to end-users working on various assays based on cfDNA (e.g. monitoring for minimal residual disease or multi-cancer early detection). Immuno-reactivity to various antigens in samples collected in Streck and EDTA tubes (from 50 patients) was assessed by peptide microarray binding assay. Feedback information on the quality of samples was shared in 900 cases. Approximately 4% of cases had failed internal pathology review, due to poor preservation, lack of adequate tissue composition, or low tumor volume and 11% failed internal nucleic acid quality assessment (DNA concetration and integrity). The blood collection tubes had no significant impact on serologic testing using peptide microarary assay. Customer feedback data showed that overall success rate exceeded 85%. Type of blood collection tubes (Streck vs EDTA) had no impact on serologic testing and studies looking at predicted neoantigens based on NGS data can be confirmed using peptide epitope arrays. Blood collected for NGS works without compromise on peptide arrays. Future validation of other collection tubes for blood or other types of fluids is needed to confirm their adequacy for specific assays. Citation Format: Zoran Gatalica, Phillip Stafford, Chris Diehnelt, Inga Rose. Quality control of the biobanked matched tissue: Blood samples for the development of diverse liquid based molecular assays [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr A013.

Polytetrafluoroethylene (PTFE) Microplastics Affect Angiogenesis and Central Nervous System (CNS) Development of Duck Embryo

Prolonged exposure to teratogens is known to cause neural tube defects (NTDs), a severe malformation of the central nervous system (CNS) that significantly contributes to global infant mortality. In recent years, exposure to nanoplastics (NPs) has been linked to faulty neural crest closure and altered neurulation by altering cellular adhesion molecules and accumulation of plastic particles in the neural tube leading to NTDs. However, research on the influence of various types of microplastics (MPs) on malformations of the CNS are still limited. In this study, we investigated whether MPs of polytetrafluoroethylene (PTFE)—a type of plastic commonly used as non-stick coatings of cooking utensils can affect angiogenesis and CNS development using ducks as model organisms. PTFE MPs were administered on Day 3 of duck embryo development at varying concentrations (0.01 mg/ml, 0.1 mg/ml, 1 mg/ml, and 5 mg/ml), and angiogenesis was evaluated using a chorioallantoic membrane (CAM) assay. Gross morphology and histology of the spinal column and brain were analyzed on Days 8 and 18, respectively. FTIR confirmed PTFE's structure, while SEM and DLS analyses showed particle sizes between 300 nm and 5 μm, classifying them as MPs. High concentrations (5 mg/ml) of PTFE MPs treated on duck embryos resulted in a 35% mortality rate and reduced vascular density, suggesting anti-angiogenic effects. Brain and spinal abnormalities, such as encephalomalacia and spinal cord discontinuities were observed in the PTFE-treated embryos. Based on these results, PTFE is an anti-angiogenic and teratogenic agent affecting the development of duck embryos.

Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners.

Peripheral blood mononuclear cells (PBMCs) are commonly used in biomedical research on the immune system and its response to disease and pathogens. This detailed protocol describes the equipment, supplies, and steps for isolating, cryopreserving, and thawing high-quality and highly viable PBMCs from whole blood cells suitable for downstream applications such as flow cytometry and RNA-sequencing. Protocols for processing plasma and buffy coat from the whole blood in parallel and concurrently with PBMCs are also described. This easy-to-follow step-by-step protocol, which utilizes density gradient centrifugation to isolate PBMCs, is accompanied by a checklist of supplies, equipment, and preparation steps. This protocol is suitable for individuals with any prior experience with laboratory techniques and can be implemented in clinical or research laboratories. High-quality cell viability and RNA sequencing resulted from PBMCs collected by operators with no prior laboratory experience using this protocol.

ОЦЕНКА ВЛИЯНИЯ РЕЖИМОВ ШЛИФОВАНИЯ НА ШЕРОХОВАТОСТЬ ПЛАЗМЕННЫХ ПОКРЫТИЙ

The equipment for laboratory tests during grinding of plasma coatings is presented. The results of the study of the influence of the modes of mechanical treatment with an abrasive tool on the quality of the surface of plasma coatings are presented. Three materials were applied to the surfaces - based on iron, nickel and self-fluxing powder for plasma spraying, with their subsequent grinding and study of the surface quality.

Highly Sensitive Molecular Diagnostic Platform for Scrub Typhus Diagnosis Using O. tsutsugamushi Enrichment and Nucleic Acid Extraction.

Scrub typhus is caused by the Gram-negative obligate intracellular bacterium Orientia tsutsugamushi, and this tick-borne disease is difficult to distinguish from other acute febrile illnesses as it typically presents with symptoms such as rash, crusting at the bite site, headache, myalgia, lymphadenopathy, and elevated liver transaminases. It can often be diagnosed clinically, but not all patients present with characteristic symptoms, so serological diagnosis and molecular techniques may be required. However, existing diagnostic tests often have low sensitivity and specificity, making early detection difficult. This study presents a nucleic acid extraction method using large volumes of plasma and buffy coat to increase sensitivity, as well as an improved detection method using two target genes. Using the I-PULL device, nucleic acids can be extracted from up to 4 mL of sample in 30 min, avoiding contamination. The extracted DNA detects two genes of O. tsutsugamushi, increasing sensitivity compared to single-gene detection. Clinical validation in 38 patient samples showed 100% specificity and 95.24% sensitivity for the single target gene, with specificity and sensitivity rising to 100% when both genes are analyzed. This molecular diagnostic platform can be useful for distinguishing scrub typhus from similar diseases.

Teflon flu: Is it a rising concern of only developed country?

Abstract: Teflon flu also known as polymer fume fever is a temporary flu-like illness caused by inhaling fumes released when polytetrafluoroethylene (PTFE), the chemical used to make non-stick coatings like Teflon, is heated to very high temperatures. This condition typically occurs when non-stick cookware is overheated to above 500°F (260°C), releasing fumes that can cause symptoms in humans. Teflon flu is manifested with fever, chills, headache, body aches, cough, sore throat and shortness of breath which usually appear within a few hours of exposure and may last for a day or tw. Teflon flu is generally not considered dangerous for healthy individuals but repeated exposure could have more serious effects. Although in India cases are not widely reported as in countries like the U.S., India’s medical community, including the Indian Council of Medical Research has advised caution and advocated use of earthen pots for cooking. Here we discuss in detail the causes, and ways to prevent Teflon flu in Indian context.

Modelling and Experimental Validation of the Flame Temperature Profile in Atmospheric Plasma Coating Processes on the Substrate

This work presents a characterisation model for the temperature distribution at different substrate depths during the atmospheric plasma spray (APS) coating process. The torch heat flow in this model is simulated as forced convection defined by a surface, a temperature profile, and a convection coefficient. The simulation model considers three plasma temperature profiles of the Al2O3 coating on a 5 mm thickness flat aluminium substrate. The simple and low-cost experimental procedure, based on a thermocouple, measures the plasma plume temperature distribution of the APS coating system, and their results are used to obtain the parameter values of each of the three proposed plasma temperature profiles. The experimental method for in situ non-contact temperature measurements inside the substrate is based on an infrared pyrometry technique and validates the simulation results. The Gaussian temperature profile shows excellent accuracy with the measured temperatures. The Gaussian approach could be a powerful tool for predicting residual stress through a coupled one-way thermal-mechanical analysis of the APS process.

Abstract A031: Methylomic Biomarkers Associated with Prostate Cancer in Black Men

Abstract Introduction: The prostate cancer (PCa) disparity remains the highest cancer disparity in the US, with Black men facing an estimated 78% higher incidence and 230% higher mortality than White men. However, current understanding of risk factors for PCa such as socioeconomic status (SES), diet, and genetics cannot fully explain these disparities. Our objective is to examine DNA methylation (DNAm) markers in a cohort of Black men and assess their associations with PCa status. Methods: We randomly selected 100 Black men for DNAm profiling (50 cases diagnosed with clinically significant (Gleason Grade 3+) prostate cancer and 50 age-matched controls) from two larger cohorts. The cohorts recruited 400 Black men who were newly diagnosed with sporadic PCa as well as 400 controls matched on age and race. All men were 40-79 at recruitment and were enrolled from urology clinics at five Chicago-area medical institutions. All PCa cases underwent a transrectal prostate biopsy for abnormal prostate-specific antigen (PSA) or digital rectal exam and had a histologically confirmed prostate cancer and were not previously diagnosed with PCa. All men in the control group had been recruited from community prostate cancer screening events or were referred to participating urology clinics and had a negative prostate biopsy for a PSA <10ng/ml and were not diagnosed with PCa for 2 years of follow-up. Men with prior PCa diagnosis or conditions known to affect PSA levels were excluded. After enrollment and informed consent, all men provided demographic and lifestyle data via three questionnaires, followed by venipuncture for DNA collection. These specimens were obtained using buffy coat extraction after red blood cell lysis, centrifugation, and pipetting off the overlying plasma and buffy coat for freezing followed by Illumina EPIC arrays for DNAm assays. We examined DNAm markers in gene promoter regions associated with PCa using logistic regression and adjusted for multiple testing using Bonferroni correction. All models controlled for age, BMI, smoking status, first degree family history of PCa, blood cell types, and technical control variables. Results: We found 2,180 CpG sites in gene promoter regions associated with any PCa in this population. The top ten strongest associations identified in our models include loci on genes that have already been associated with prostate cancer progression (MAFG, FAM65B, OSM, PRKAG2), risk (SLC11A1, LAT), and metastasis (S1PR4) as well as immune system functioning (RAP1GAP2). Conclusions: Our initial findings suggest that DNAm markers may be useful predictors of clinically significant PCa status among Black men. Additional research is currently ongoing to expand the sample size of the DNAm profiling and to explore links between these loci and social determinants of health, as well as associations with more advanced (i.e., higher Gleason Grade) PCa. Citation Format: Brian T. Joyce, Yishu Qu, Jun Wang, Kai Zhang, Zequn Sun, Lifang Hou, Adam B. Murphy. Methylomic Biomarkers Associated with Prostate Cancer in Black Men [abstract]. In: Proceedings of the 17th AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2024 Sep 21-24; Los Angeles, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2024;33(9 Suppl):Abstract nr A031.

Plasma Coating for Hydrophobisation of Micro- and Nanotextured Electrocatalyst Materials

The need for sustainable energy solutions is steering research towards green fuels. One promising approach involves electrocatalytic gas conversion, which requires efficient catalyst surfaces. This study focuses on developing and testing a hydrophobic octadiene (OD) coating for potential use in electrocatalytic gas conversion. The approach aims to combine a plasma-deposited hydrophobic coating with air-trapping micro- and nanotopographies to increase the yield of electrocatalytic reactions. Plasma polymerisation was used to deposit OD films, chosen for their fluorine-free non-polar properties, onto titanium substrates. We assessed the stability and charge permeability of these hydrophobic coatings under electrochemical conditions relevant to electrocatalysis. Our findings indicate that plasma-deposited OD films, combined with micro-texturing, could improve the availability of reactant gases at the catalyst surface while limiting water access. In the presence of nanotextures, however, the OD-coated catalyst did not retain its hydrophobicity. This approach holds promise to inform the future development of catalyst materials for the electrocatalytic conversion of dinitrogen (N2) and carbon dioxide (CO2) into green fuels.

Alliance A022104/NRG-GI010: The Janus Rectal Cancer Trial: a randomized phase II/III trial testing the efficacy of triplet versus doublet chemotherapy regarding clinical complete response and disease-free survival in patients with locally advanced rectal cancer

BackgroundRecent data have demonstrated that in locally advanced rectal cancer (LARC), a total neoadjuvant therapy (TNT) approach improves compliance with chemotherapy and increases rates of tumor response compared to neoadjuvant chemoradiation (CRT) alone. They further indicate that the optimal sequencing of TNT involves consolidation (rather than induction) chemotherapy to optimize complete response rates. Data, largely from retrospective studies, have also shown that patients with clinical complete response (cCR) after TNT may be managed safely with the watch and wait approach (WW) instead of preemptive total mesorectal resection (TME). However, the optimal consolidation chemotherapy regimen to achieve cCR has not been established, and a randomized clinical trial has not robustly evaluated cCR as a primary endpoint. Collaborating with a multidisciplinary oncology team and patient groups, we designed this NCI-sponsored study of chemotherapy intensification to address these issues and to drive up cCR rates, to provide opportunity for organ preservation, improve quality of life for patients and improve survival outcomes.MethodsIn this NCI-sponsored multi-group randomized, seamless phase II/III trial (1:1), up to 760 patients with LARC, T4N0, any T with node positive disease (any T, N +) or T3N0 requiring abdominoperineal resection or coloanal anastomosis and distal margin within 12 cm of anal verge will be enrolled. Stratification factors include tumor stage (T4 vs T1-3), nodal stage (N + vs N0) and distance from anal verge (0–4; 4–8; 8–12 cm). Patients will be randomized to receive neoadjuvant long-course chemoradiation (LCRT) followed by consolidation doublet (mFOLFOX6 or CAPOX) or triplet chemotherapy (mFOLFIRINOX) for 3–4 months. LCRT in both arms involves 4500 cGy in 25 fractions over 5 weeks + 900 cGy boost in 5 fractions with a fluoropyrimidine (capecitabine preferred). Patients will undergo assessment 8–12 (± 4) weeks post-TNT completion. The primary endpoint for the phase II portion will compare cCR between treatment arms. A total number of 312 evaluable patients (156 per arm) will provide statistical power of 90.5% to detect a 17% increase in cCR rate, at a one-sided alpha = 0.048. The primary endpoint for the phase III portion will compare disease-free survival (DFS) between treatment arms. A total of 285 DFS events will provide 85% power to detect an effect size of hazard ratio 0.70 at a one-sided alpha of 0.025, requiring enrollment of 760 patients (380 per arm). Secondary objectives include time-to event outcomes (overall survival, organ preservation time and time to distant metastasis) and adverse event rates. Biospecimens including archival tumor tissue, plasma and buffy coat, and serial rectal MRIs will be collected for exploratory correlative research. This study, activated in late 2022, is open across the NCTN and had accrued 330 patients as of May 2024. Study support: U10CA180821, U10CA180882, U24 CA196171; https://acknowledgments.alliancefound.org.DiscussionBuilding on data from modern day rectal cancer trials and patient input from national advocacy groups, we have designed The Janus Rectal Cancer Trial studying chemotherapy intensification via a consolidation chemotherapy approach with the intent to enhance cCR and DFS rates, increase organ preservation rates, and improve quality of life for patients with rectal cancer.Trial registrationClinicaltrials.gov ID: NCT05610163; Support includes U10CA180868 (NRG) and U10CA180888 (SWOG).

Experiment Evaluation on the Porosity of Plasma Coating from Ceramic Al2O3 – TiO2 Using the Metallography

Background: The objective of this paper is an experimental investigation of the metallographic porosity of plasma coating using standard materials from the ceramic system Al2O3-TiO2 on the substrate of steel SS400 at laboratory scale Lab at the Research Institute of Mechanical Engineering. Contribution: The main contribution of the research is the evaluation of the porosity of the coating under the influence of the main technological parameters since the porosity plays a significant role in the performance of the working surface using the built-in Image Pro-Analyzer software in the Axiovert 25 MAT microscope. Method: The spraying material is Al2O3-TiO2 powder. The input parameters are: spraying distance (Lp; current of plasma stream (Ip); powder feed flow (Gp and spraying rate (Vp). Results: The findings show that the average porosity of the Al2O3-TiO2 plasma coating is inversely decreasing in the increasing direction of the distance: spray in the range Lp = 100–200 mm, increase proportional to the increase of the current plasma flow rate in the range Ip = 400–600 A, provided that the powder feed flow Gp = 1.7 kg/h and the spraying rate Vp = 50 mm/min. Conclusion: The degree of influence of the three main technological parameters (Lp, Ip, and Gp) in the survey limit domain may be due to the selected Lp spray distance within the relatively wide recommended range of the plasma spraying equipment supplier. It is recommended to conduct the experimental planning of the L27 type to localize the optimum area.

Evaluation of platelet concentrates prepared using different methods after overnight holding (18-24 h) of whole blood at room temperature.

Regulatory requirement of fixed holding time (6 h) of whole blood (WB) at room temperature, that is, 22-24°C (RT) results in sub-optimal component separation. The aim was to evaluate the platelet concentrates (PC) prepared by both platelet rich plasma (PRP) and buffy coat (BC) methods after overnight hold (18-24 h) at RT. A prospective experimental study was performed. A total of 48 WB units collected were divided into four groups (12 each) control-1 (C1) and test-1 (T1) for PRP and control-2 (C2) and test-2 (T2) for the BC method. Control groups were processed within 6 h, and in test groups, components were prepared after overnight hold, followed by evaluation of quality parameters. Irrespective of the method used, all PCs had similar volume, platelet yield, swirling, no bacterial contamination, RBC contamination, PaO2 and PaCO2 levels. PCs in the T1 group had significant differences in glucose and MPV values on d1, which were resolved by d5 of storage. PCs in T2 has significant differences in pH, glucose, and MPV levels throughout storage. PRBC in test and control groups had similar quality parameters till d42 of storage. FFPs in all tests were noninferior to the concurrent control groups till 3 months of storage. Overnight holding of WB had no lasting deleterious changes. Though a few biochemical parameters in the test groups were significantly different, they can be accepted to improve the logistics of component separation. Overall PRP method seemed to have a better result than the BC method after an overnight hold.

Application of new plasma coatings for restoration of the surface of material

Application of new plasma coatings for restoration of the surface of material

The Study of the Content of Oxygen and Nitrogen in Plasma Coatings

The Study of the Content of Oxygen and Nitrogen in Plasma Coatings

Development and properties of new powder materials

Problem. The study analyzed powder materials to obtain wear-resistant coatings. Field tests for wear resistance were conducted using the method of friction between two surfaces. The tests were carried out on the universal test bench SMC-2 at a rotation speed of 200 rpm. A pressure of 100 kgf was applied to the test sample. Goal. The wear resistance test cycle comprised 200,000 revolutions, with lubrication provided through the drop method. Special powders were used in the experiment, including PG-SR3, PN55T45, and PR-Ni70Cr17S4R4 + 20% PT-Al5N with a PT-Al5N under layer. Methodology. The selection of wear-resistant coatings was based on the analysis of the following properties: adhesion strength of the coating to the base material, wear resistance of the coatings, hardness, and porosity. Results. Metallographic analysis of the structure of plasma coatings obtained from powders PG-SR3, PN55T45, and PR-Ni70Cr17S4R4 + 20% PT-All5N with three different plasma heater power levels (10 kW, 15 kW, and 20 kW) indicated that the densest and most reliable coating among those studied is the coating made from PR-Ni70Cr17S4R4 + 20% PT-Al5N powder. Practical value. Therefore, there is no need to increase the heater power beyond 15 kW when spraying this type of powder. The research concluded that when obtaining wear-resistant coatings, the optimal choice is PR-Ni70Cr17S4R4 + 20% PT-All5N powder with a PT-Al5N under layer.

Antibacterial Plasma Coating with Aggregation‐Induced Emission Photosensitizers to Prevent Surgical Site Infections (Adv. Mater. Interfaces 15/2024)

Antibacterial Plasma Coating with Aggregation‐Induced Emission Photosensitizers to Prevent Surgical Site Infections (Adv. Mater. Interfaces 15/2024)

The Study of the Content of Oxygen and Nitrogen in Nickel-Based Plasma Coatings

The Study of the Content of Oxygen and Nitrogen in Nickel-Based Plasma Coatings

Structural Changes in Hydroxyapatite Plasma Coatings Depending on the Plasma Power

Structural Changes in Hydroxyapatite Plasma Coatings Depending on the Plasma Power

Effect of viscosity on bubble spreading on flat solid surfaces

The behavior and dynamics of bubble spreading has a great impact on interfacial contact area and time, which is significant for the heat/mass transfer or separation efficiency. In this work, the dynamical behavior of bubble spreading especially the three-phase contact line (TPL) on solid flat surfaces in low viscous liquids was investigated. The results show that the final length of TPL on homogeneous solid surfaces tend to decrease with the increasing liquid viscosity. For heterogenous solid surfaces, TPL motion on non-stick coal surface is basically the same as that on homogeneous solid surfaces, whereas that on anthracite surface shows an anomalous morphology. By applying the power-law model, the fast and slow spreading stages of bubble on homogeneous surfaces show the universal exponent of b = 1/2 and b = 1/10, respectively. While the exponent for bubble spreading on heterogeneous surface is complicated due to the special surface physico-chemical properties.

Abstract 2300: AI enabled whole exome& transcriptome liquid biopsy addressing MCED, MRD, and therapy selection on a single platform

Abstract Effective clinical management of cancer patients requires an accurate and early diagnosis, highly sensitive monitoring of minimal residual disease (MRD), and precise therapy selection. There are multiple tests available that attempt to address each of these needs independently with varying degrees of clinical utility. Caris Assure is a proprietary circulating nucleic acid sequencing platform that couples whole exome and transcriptome (WES/WTS) sequencing on white blood cells and plasma with advanced machine learning techniques to satisfy all three testing needs on one platform. This test detects SNVs, INDELs, structural variants, copy number, gene expression, tumor mutational burden (TMB), microsatellite instability (MSI), fragment length, and aneuploidy of both somatic (tumor and clonal hematopoiesis) and germline origin. Caris’ extensive database of over 350,000 tissue WES/WTS from solid malignancies was used to train deep learning neural networks to identify the molecular underpinnings of cancer. These networks were then deployed on WES/WTS data from plasma and buffy coat in pursuit of signals that can inform early detection, MRD and therapy selection. Validation studies were performed to characterize the analytic and clinical performance of Assure on over 3000 patient blood samples. These samples include ~1000 non-cancer patients (controls), ~1700 newly diagnosed patients where blood was collected at surgery (early detection), ~500 early-stage patients during adjuvant therapy at multiple time points (MRD), and ~200 locally advanced/metastatic patients where matched tissue testing was also performed (therapy selection). For early detection, stratification of blood samples from patients with stage I-IV cancer versus those with no reported cancer resulted in an AUC > 0.99 and included over 30 types of solid tumors. Notably, at 99.5% specificity, the sensitivities for stages I-IV (n= 119, 54, 50, 27) were 73%, 80%, 76%, and 89%. In the MRD setting for high-risk patients, the disease-free survival of patients whose cancers were predicted to recur was significantly shorter (39.6 mo) than those predicted not to recur (93.4 mo) (HR: 5.18, 95%CI: 2.94-9.09, p<.00001). This performance was observed across multiple lineages of cancer including but not limited to breast, colon, lung, and bladder. Lastly, for therapy selection, detection of driver mutations where blood was collected within 30 days of matched tissue demonstrated high concordance with a PPA of 93.8% and PPV of 96.8%. CHIP correction proved to be essential as ~35% percent of patients had CHIP mutations, including KRAS, BRAF, ATM, BRCA1/2, findings that could lead to improper therapy selection. Herein, we demonstrate for the first time a single liquid biopsy assay that addresses the entire continuum of care in clinical oncology with optimal diagnostic, prognostic, and predictive utility for patients and physicians. Citation Format: Jim Abraham, Valeriy Domenyuk, Maria Perdigones Borderias, Takayuki Yoshino, Elisabeth I. Heath, Emil Lou, Stephen Liu, John Marshall, Wafik S. El-Deiry, Anthony Shields, Martin Dietrich, Yoshiaki Nakamura, Takao Fujisawa, David D. Halbert, Dominic Sacchetti, Seth Stahl, Adam Stark, Sergey Klimov, Sourabh Antani, Chadi Nabhan, Jeffrey Swensen, George Poste, Matt Oberley, Milan Radovich, George W. Sledge, David Spetzler. AI enabled whole exome& transcriptome liquid biopsy addressing MCED, MRD, and therapy selection on a single platform [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 2300.