Related Plant Species Research Articles

Butterfly eggs prime anti-herbivore defense in an annual but not perennial Arabidopsis species

Main conclusionUnlike Arabidopsis thaliana, defenses of Arabidopsis lyrata against Pieris brassicae larval feeding are not primable by P. brassicae eggs. Thus, egg primability of plant anti-herbivore defenses is not phylogenetically conserved in the genus Arabidopsis.While plant anti-herbivore defenses of the annual species Arabidopsis thaliana were shown to be primable by Pieris brassicae eggs, the primability of the phylogenetically closely related perennial Arabidopsis lyrata has not yet been investigated. Previous studies revealed that closely related wild Brassicaceae plant species, the annual Brassica nigra and the perennial B. oleracea, exhibit an egg-primable defense trait, even though they have different life spans. Here, we tested whether P. brassicae eggs prime anti-herbivore defenses of the perennial A. lyrata. We exposed A. lyrata to P. brassicae eggs and larval feeding and assessed their primability by (i) determining the biomass of P. brassicae larvae after feeding on plants with and without prior P. brassicae egg deposition and (ii) investigating the plant transcriptomic response after egg deposition and/or larval feeding. For comparison, these studies were also conducted with A. thaliana. Consistent with previous findings, A. thaliana’s response to prior P. brassicae egg deposition negatively affected conspecific larvae feeding upon A. thaliana. However, this was not observed in A. lyrata. Arabidopsis thaliana responded to P. brassicae eggs with strong transcriptional reprogramming, whereas A. lyrata responses to eggs were negligible. In response to larval feeding, A. lyrata exhibited a greater transcriptome change compared to A. thaliana. Among the strongly feeding-induced A. lyrata genes were those that are egg-primed in feeding-induced A. thaliana, i.e., CAX3, PR1, PR5, and PDF1.4. These results suggest that A. lyrata has evolved a robust feeding response that is independent from prior egg exposure.

Genomic implications of the repeated shift to self-fertilization across a species' geographic distribution.

The ability to self-fertilize often varies among closely related hermaphroditic plant species, though, variation can also exist within species. In the North American Arabidopsis lyrata, the shift from self-incompatibility (SI) to selfing established in multiple regions independently, mostly since recent postglacial range expansion. This has made the species an ideal model for the investigation of the genomic underpinnings of the breakdown of SI and its population genetic consequences. By comparing nearby selfing and outcrossing populations across the entire species' geographic distribution, we investigated variation at the self-incompatibility (S-)locus and across the genome. Furthermore, a diallel crossing experiment on one mixed-mating population was performed to gain insight in the genetics of mating system variation. We confirmed that the breakdown of SI had evolved in several S-locus backgrounds. The diallel suggested the involvement of binuclearly expressed parental genes with dominance relations. Though, the population-level genome-wide association study did not single out clear-cut candidate genes but several regions with one near the S-locus. On the implication side, selfing as compared to outcrossing populations had less than half of the genomic diversity, while the number of runs of homozygosity and their length scaled with the degree of inbreeding. The results highlight that mating system shifts to selfing, its genetic underpinning and the likely negative genomic consequences for evolutionary potential can be strongly interlinked with past range dynamics.

Eco-evolutionary factors contribute to chemodiversity in aboveground and belowground cucurbit herbivore-induced plant volatiles.

When attacked by insect herbivores, plants emit blends of chemical compounds known as herbivore-induced plant volatiles (HIPVs). Although HIPVs are produced both aboveground and belowground, how HIPVs vary across plant tissues remains unresolved, as do the selective forces shaping interspecific HIPV emission patterns. Here, we compared foliar and root HIPVs within and among closely related plant species and evaluated if different eco-evolutionary forces, including plant domestication, coexistence histories with herbivores, or phylogenetic relatedness, explain HIPV blends. To examine aboveground and belowground patterns in HIPVs, we compared leaf and root volatile profiles for six species in the Cucurbitaceae that differed in domestication status and coexistence history with specialist insect herbivores. We predicted that within-species HIPVs from different tissues would be more similar than HIPV blends among different species, and that plant volatile chemodiversity was reduced by domestication and enhanced by coexistence histories with herbivores. We found that herbivory induced both quantitative and qualitative changes in volatile emissions across all plant species, which were more pronounced aboveground than belowground. Each species produced tissue-specific HIPVs, and foliar and root HIPVs differed among species. Contrary to our predictions, plant domestication enhanced foliar volatile diversity, while coexistence histories with herbivores reduced foliar and root volatile diversity. Additionally, phylogenetic relatedness did not correlate with aboveground or belowground volatiles. Overall, this work furthers our understanding of the eco-evolutionary forces driving patterns in aboveground and belowground HIPV emissions, elucidating an important and previously undescribed component of within-plant variation in chemodiversity.

Chromosome-specific painting reveals the Y genome origin and chromosome rearrangements of the St genome in Triticeae.

Karyotypes provide key cytogenetic information on phylogenetic relationships and evolutionary origins in related plant species. The St genome of Pseudoroegneria contributes to eight alloploid genera, representing over half of the species that are highly valuable for wheat (Triticum aestivum) breeding and for understanding Triticeae species evolution. However, St chromosome characterization is challenging due to limited cytogenetic markers and DNA information. We developed a complete set of St genome-specific chromosome painting probes for identification of the individual chromosomes 1St to 7St based on the genome sequences of Pse. libanotica and wheat. We revealed the conservation of St chromosomes in St-containing species by chromosome painting, including Pseudoroegneria, Roegneria, Elymus, and Campeiostachys. Notably, the Y genome showed hybridization signals, albeit weaker than those of the St genome. The awnless species harboring the Y genome exhibited more intense hybridization signals compare to the awned species in Roegneria and Campeiostachys, yet weaker than the hybridization signals of the St genome in autotetraploid Pse. strigosa. Although awnless species were morphologically more similar to each other, phenotypic divergence progressively increased from awnless to awned species. Our results indicate that the Y genome originated from the St genome and shed light on the possible origin of the Roegneria and Campeiostachys species, enhancing our understanding of St-genome-containing species evolution.

Examining the structure of plant-lemur interactions in the face of imperfect knowledge.

Biotic interactions, such as plant-animal seed dispersal mutualisms, are essential for ecosystem function. Such interactions are threatened by the possible extinction of the animal partners. Using a data set that includes plant-lemur interactions across Madagascar, we studied the current state of knowledge of these interactions and their structure to determine which plant species are most at risk of losing dispersal services due to the loss of lemurs. We found substantial gaps in understanding of plant-lemur interactions; data were substantially skewed toward a few lemur species and locations. There was also a large gap in knowledge on the interactions of plants and small-bodied or nocturnal lemurs and lemurs outside a few highly studied locations. Of the recorded interactions, a significant portion occurred between lemurs and endemic plants, rather than native or introduced plants. We also found that lemur species tended to primarily consume closely related plant species. Such interaction patterns may indicate the threats to Malagasy endemic plants and highlight how lemur population loss or reductions could affect plant phylogenetic diversity. When examining the impacts of lemur extinction, losing critically endangered species left 164 plant species with no known lemur frugivore partners. Despite phylogenetic patterns in lemur diet, plants for which the only known lemur frugivore is critically endangered were not closely related. These results emphasize the need for further studies to complete our knowledge on these essential interactions and to inform conservation priorities.

In vitro evaluation of antiviral activity in carnivorous plant species

Sarracenia purpurea is a carnivorous plant historically used to treat smallpox infections. Our previous data found S. purpurea had broad spectrum antiviral activity in vitro. S. purpurea is one of several hundred identified carnivorous species of plants. Carnivorous plants have evolved through convergent evolution in at least ten independent events, usually in response to harsh environments where nutrition from prey is required for growth. These prey are known vectors of plant viruses that might introduce novel biotic stressors and defense pathways in carnivorous plants. This study evaluated the antiviral activity of several non-carnivorous and carnivorous plants from four evolutionarily distinct clades. Results demonstrated that carnivorous plants have evolved antiviral activity, a trait that is not present in related species of non-carnivorous plants. The antiviral trait may be due to the plant-prey relationship with insect vectors and an evolutionary need for carnivorous plants to have more robust antiviral defense systems.

Medicinal chemistry perspective on the structure-activity relationship of stilbene derivatives.

Stilbenes are a small family of polyphenolic secondary metabolites produced in a variety of closely related plant species. These compounds function as phytoalexins, aiding plant defense against phytopathogens and plants' adaptation to abiotic environmental factors. Structurally, some important phenolic compounds have a 14-carbon skeleton and usually have two isomeric forms, Z and E. Stilbenes contain two benzene rings linked by a molecule of ethanol or ethylene. Some derivatives of natural (poly)phenolic stilbenes such as resveratrol, pterostilbene, and combretastatin A-4 have shown various biological activities, such as anti-microbial, anti-cancer, and anti-inflammatory properties as well as protection against heart disease, Alzheimer's disease, and diabetes. Among stilbenes, resveratrol is certainly the most popular and extensively studied for its health properties. In recent years, an increasing number of stilbene compounds have been investigated for their bioactivity. This review focuses on the assessment of synthetic stilbene derivatives in terms of their biological activities and structure-activity relationship. The goal of this study is to consider the structural changes and different substitutions on phenyl rings that can improve the desired medicinal effects of stilbene-based compounds beyond the usual standards and subsequently discover biological activities by identifying effective alternatives of the evaluated compounds.

Flora of the riverside-water side of the lower section of the Nistru river and its tributaries on the territory of the Republic of Moldova

The studied section of the river. The Dniester and its tributaries are represented by 113 species, 103 genera from 55 families of higher plants (Magnoliophyta). The greatest diversity is found in the Asteraceae (21 species) and Poaceae (8 species) families. About 1/3 of the total number of species are represented by the Eurasian element. The spontaneous element of the studied flora is represented by 54 species, and the ruderal element by 36 species. Invasive elements are represented by the species Ailanthus altissima (Mill.) Swingle, Acer negundo L. Amorpha fruticosa L., Ambrosia artemisiifolia L., Grindelia squarrosa Willd.. It was also carried out for all species: determination of the percentage ratio of life forms, biological indices, ecological groups in relation to humidity, the ratio of plant species by origin, the distribution of indicator species by nitrogen (N) in percentage terms and the ratio of plant species in relation to the category trophicity.

Phytochemical screening, antimicrobial, and antioxidant properties of Helianthus annuus and Hyophila involuta: A comparative account

Angiosperms and bryophytes, though distantly related plant groups, have many similar ecological and economic implications, including medicinal value. Therefore, this study aimed to analyse the phenolic and flavonoids composition and antioxidant and antimicrobial activities of Helianthus annuus L. (Angiosperms-Asteraceae) and another plant, a moss (Bryophyta), Hyophila involuta (Hook.) Jaeg., aerial parts (leaves), prepared in four different extracts (methanol, chloroform, distilled water, and petroleum ether). Phytochemical screening was conducted using standard methods of precipitation and colouration reactions. The Folin-Ciocalteu method was employed to determine the total phenol content, while the Aluminium Chloride Colorimetric method was used for flavonoid content determination. The antioxidant activity was measured through two methods: DPPH and NOSA scavenging activity. The phytochemical screening detected the presence and absence of fixed oils and fats, flavonoids, saponins, terpenoids, tannins, polyphenols, carbohydrates, and glycosides in both plants. The antibacterial and antifungal activity of both plants' methanolic extracts was examined against bacterial and fungal pathogens, i.e., Escherichia coli and Bacillus subtilis and fungal strains, i.e., Fusarium oxysporum and Aspergillus niger. The results were compared to a regular antibiotic disc and negative control that served as a methanol solvent. The methanolic extract of H. annuus has higher total phenol and flavonoid content, as well as antioxidant and antimicrobial activity, than H. involuta. Based on these data, it can be concluded that, while H. annuus is more effective than H. involuta, both distantly related plant species have similar phytochemical profiles and should be included equally in future herbal compositions.

Genome-Wide Identification and Expression Analysis of the Broad-Complex, Tramtrack, and Bric-à-Brac Domain-Containing Protein Gene Family in Potato

The BTB (broad-complex, tramtrack, and bric-à-brac) domain, also known as the POZ (POX virus and zinc finger) domain, is a conserved protein–protein interaction domain present in various organisms. In this study, we conducted a genome-wide search to identify and characterize BTB genes in Solanum tuberosum. A total of 57 StBTBs were identified and analyzed for their physicochemical properties, chromosomal distribution, gene structure, conserved motifs, phylogenetic relationships, tissue-specific expression patterns, and responses to hormonal and stress treatments. We found that StBTBs were unevenly distributed across potato chromosomes and exhibited diverse gene structures and conserved motifs. Tissue-specific expression analysis revealed differential expression patterns across various potato tissues, implying their roles in plant growth and development. Furthermore, differential expression analysis under hormonal and stress treatments indicated the involvement of StBTBs in abiotic and biotic stress responses and hormone signaling pathways. Protein–protein interaction analysis identified potential interactions with ribosomal proteins, suggesting roles in translational regulation. Additionally, microRNA target site analysis revealed regulatory relationships between StBTBs and miRNAs. Our study provides a comprehensive understanding of the StBTB gene family in potato, laying the groundwork for further functional characterization and manipulation of these genes to improve stress tolerance and agricultural productivity in potato and related plant species.

Centella asiatica (L.) Urb. and Hydrocotyle umbellata L. identification and quality assessment: A methodology comparison.

Centella is an important genus in the Apiaceae family. It includes Centella asiatica, which has significant edible and medicinal values. However, this species is easily confused due to its similar morphological traits to Hydrocotyle umbellata, hindering its utilization in the consumer and pharmacological industries. The study aims to differentiate these two closely related plant species using reliable methods of confirming the authenticity of natural herbal medicines. Our work mainly focuses on the basic morphological characteristics, chemical markers, genetic fingerprints, and their biological responses. The plants can be clearly differentiated using their leaf shapes, stipules, petioles, inflorescences, and fruit structures. Although the phytochemical compositions of the C. asiatica extract were similar to that of H. umbellata which included flavonoids, tannins, and saponins important to the plant's ability to reduce inflammation and promote healing of wounds, the H. umbellata extract showed significantly higher toxicity than that of C. asiatica. High-performance liquid chromatography analysis was used to identify chemical fingerprints. The result revealed that C. asiatica had major triterpene glycoside constituents including asiaticoside, asiatic acid, madecassoside, and madecassic acid, which have a wide range of medicinal values. In contrast, triterpenoid saponins were not identified in H. umbellata. Furthermore, using SCoT1-6 primers was possible to effectively and sufficiently created a dendrogram which successfully identified the closeness of the plants and confirmed the differences between the two plant species. Therefore, differentiation can be achieved through the combination of morphometrics, molecular bioactivity, and chemical analysis.

Genome-wide analysis of the poplar homeobox gene family and the identification of PtHB180 as an important regulator of plant developmental processes and drought stress response

Homeobox (HB) transcription factors have been reported to regulate various developmental processes and hormone signaling and mediate plant adaption to abiotic stresses, including drought. As a major subfamily of HB family, KNOX genes have been widely studied in herbaceous plants. However, the behaviors of KNOX genes in regulating plant developmental processes and stress resistance in woody plants remain poorly understood. Here, we found a KNOX family gene in the model tree species Populus trichocarpa (hereafter “poplar”), PtHB180, which enhances drought tolerance by regulating developmental processes through auxin and gibberellin pathways and mediating the ABA signaling pathway leading to rapid stomatal closure. Overexpression lines of PtHB180 (PtHB180-OE) displayed pleiotropic phenotypes, including increased plant height and decreased leaf area by affecting auxin and/or gibberellin contents and signaling. PtHB180-OE plants also displayed improved drought tolerance under short- and long-term drought stress. Results of transcriptome sequencing suggested the involvement of genes related to ABA signaling in regulating drought tolerance in PtHB180-OE. Specifically, PtHB180-OE plants showed significantly enhanced stomatal sensitivity to exogenous ABA. Taken together, our results indicate that PtHB180 enhances drought tolerance in poplar via regulating plant architecture and mediating ABA signaling to close stomata. Therefore, PtHB180 can be used as a potential candidate gene to improve drought tolerance by genetic transformation in poplar and related plant species.

Is competitive ability the key adaptation to benign environments? Revisiting experiments on closely related species of tidal plants.

A central goal in biology is to understand which traits underlie adaptation to different environments. Yet, few studies have examined the relative contribution of competitive ability towards adaptive divergence among species occupying distinct environments. Here, we test the relative importance of competitive ability as an adaptation to relatively benign versus challenging environments, using previously published studies of closely related species pairs of primarily tidal plants subjected to reciprocal removal with transplant experiments in nature. Subordinate species typically occupy more challenging environments and showed consistent evidence for adaptation to challenging conditions, with no significant competitive effect on non-local, dominant species. In contrast, dominant species typically occupy relatively benign environments and performed significantly better than non-local, subordinate species that faced competition from the dominant species. Surprisingly, when the two species were not allowed to compete, the subordinate species performed as well as the dominant species in the benign environments where the subordinate species do not occur. These results suggest that competitive ability is the most important adaptation distinguishing the species that occupy relatively benign environments. The limited scope and number of suitable experimental studies encourage future work to test if these results are generalizable across taxa and environments.

Celine, a long interspersed nuclear element retrotransposon, colonizes in the centromeres of poplar chromosomes.

Centromeres in most multicellular eukaryotes are composed of long arrays of repetitive DNA sequences. Interestingly, several transposable elements, including the well-known long terminal repeat centromeric retrotransposon of maize (CRM), were found to be enriched in functional centromeres marked by the centromeric histone H3 (CENH3). Here, we report a centromeric long interspersed nuclear element (LINE), Celine, in Populus species. Celine has colonized preferentially in the CENH3-associated chromatin of every poplar chromosome, with 84% of the Celine elements localized in the CENH3-binding domains. In contrast, only 51% of the CRM elements were bound to CENH3 domains in Populus trichocarpa. These results suggest different centromere targeting mechanisms employed by Celine and CRM elements. Nevertheless, the high target specificity seems to be detrimental to further amplification of the Celine elements, leading to a shorter life span and patchy distribution among plant species compared with the CRM elements. Using a phylogenetically guided approach, we were able to identify Celine-like LINE elements in tea plant (Camellia sinensis) and green ash tree (Fraxinus pennsylvanica). The centromeric localization of these Celine-like LINEs was confirmed in both species. We demonstrate that the centromere targeting property of Celine-like LINEs is of primitive origin and has been conserved among distantly related plant species.

New perspective for the upscaling of plant functional response to flooding stress in salt marshes using remote sensing

Understanding the response of salt marshes to flooding is crucial to foresee the fate of these fragile ecosystems, requiring an upscaling approach. In this study we related plant species and community response to multispectral indices aiming at parsing the power of remote sensing to detect the environmental stress due to flooding in lagoon salt marshes. We studied the response of Salicornia fruticosa (L.) L. and associated plant community along a flooding and soil texture gradient in nine lagoon salt marshes in northern Italy. We considered community (i.e., species richness, dry biomass, plant height, dry matter content) and individual traits (i.e., annual growth, pigments, and secondary metabolites) to analyze the effect of flooding depth and its interplay with soil properties. We also carried out a drone multispectral survey, to obtain remote sensing-derived vegetation indices for the upscaling of plant responses to flooding. Plant diversity, biomass and growth all declined as inundation depth increased. The increase of soil clay content exacerbated flooding stress shaping S. fruticosa growth and physiological responses. Multispectral indices were negatively related with flooding depth. We found key species traits rather than other community traits to better explain the variance of multispectral indices. In particular stem length and pigment content (i.e., betacyanin, carotenoids) were more effective than other community traits to predict the spectral indices in an upscaling perspective of salt marsh response to flooding. We proved multispectral indices to potentially capture plant growth and plant eco-physiological responses to flooding at the large scale. These results represent a first fundamental step to establish long term spatial monitoring of marsh acclimation to sea level rise with remote sensing. We further stressed the importance to focus on key species traits as mediators of the entire ecosystem changes, in an ecological upscaling perspective.

Plant species within Streptanthoid Complex associate with distinct microbial communities that shift to be more similar under drought.

Prolonged water stress can shift rhizoplane microbial communities, yet whether plant phylogenetic relatedness or drought tolerance predicts microbial responses is poorly understood. To explore this question, eight members of the Streptanthus clade with varying affinity to serpentine soil were subjected to three watering regimes. Rhizoplane bacterial communities were characterized using 16S rRNA gene amplicon sequencing and we compared the impact of watering treatment, soil affinity, and plant species identity on bacterial alpha and diversity. We determined which taxa were enriched among drought treatments using DESeq2 and identified features of soil affinity using random forest analysis. We show that water stress has a greater impact on microbial community structure than soil affinity or plant identity, even within a genus. Drought reduced alpha diversity overall, but plant species did not strongly differentiate alpha diversity. Watering altered the relative abundance of bacterial genera within Proteobacteria, Firmicutes, Bacteroidetes, Planctomycetes, and Acidobacteria, which responded similarly in the rhizoplane of most plant species. In addition, bacterial communities were more similar when plants received less water. Pseudarthrobacter was identified as a feature of affinity to serpentine soil while Bradyrhizobium, Chitinophaga, Rhodanobacter, and Paenibacillus were features associated with affinity to nonserpentine soils among Streptanthus. The homogenizing effect of drought on microbial communities and the increasing prevalence of Gram-negative bacteria across all plant species suggest that effects of water stress on root-associated microbiome structure may be predictable among closely related plant species that inhabit very different soil environments. The functional implications of observed changes in microbiome composition remain to be studied.

Chemotaxonomic classification of closed-related sweet oranges (Citrus sinensis L.) using principal component and cluster analysis of color and bi-oactive compounds in the orange peels

Chemotaxonomy is a systematic approach to plant classification based on discernible variations and similarities in their biochemical compositions, with a particular emphasis on secondary metabolites that often exhibit specificity within taxonomically related plant species. In the context of our investigation, we conducted a comprehensive chemotaxonomic analysis of sweet oranges utilized in concentrated juice production. This involved a thorough examination of antioxidant activities, characterization of flavonoid and phenolic profiles, UV-visible spectra scanning, and the implementation of color analysis. The results from cluster analysis demonstrated the superior efficacy of color analysis in discerning subtle distinctions among orange varieties. Further refinement in the analytical process requires the application of supplementary techniques after an exhaustive and precise extraction facilitated by organic solvents. Notably, all employed methodologies successfully discriminated between distinct orange types. While the compilation of extensive datasets presents inherent challenges, the incorporation of Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) enhances the interpretability of the obtained results. Considering that existing methodologies for assessing fresh fruit quality rely on subjective human visual inspection or orange juice analysis by ratio, which may lack precision in quantifying chemical constituents, there exists a growing demand for expedited techniques that can reliably ensure the quality of orange juice within the industry.

Host plants and nymph morphology of an endemic treehopper, Pyrgonota bifoliata, in the Mount Makiling Forest Reserve, Luzon, Philippines

ABSTRACT Pyrgonota bifoliata (Insecta: Hemiptera: Membracidae) is a distinct endemic Philippine treehopper species complex distinguished by its extremely elevated and bifurcated pronotal structures. An earlier study suggested that P. bifoliata is a host specialist that feeds on Bridelia (Phyllanthaceae) in northern Luzon and Rubus (Rosaceae) in southern Mindanao. However, the host plant range across the geographical distribution of the P. bifoliata species complex is still poorly understood. In this study, field observations and DNA barcoding were performed to determine the host plant range of P. bifoliata in the Mount Makiling Forest Reserve of Laguna Province on the island of Luzon. The morphology of the last-instar nymph of P. bifoliata was described to facilitate the identification of the species and its host plants. Behavioural interactions between P. bifoliata and its associated ants were also recorded. The results revealed that Piper umbellatum (Piperaceae) and Saurauia latibractea (Actinidiaceae) were the major host plants used by both nymphs and adults of P. bifoliata. The findings indicate that P. bifoliata in the Mount Makiling Forest Reserve of central Luzon is a polyphagous host generalist that primarily utilises two distantly related plant species. The adults of P. bifoliata also occur on Alangium longiflorum (Cornaceae) and Ficus ulmifolia (Moraceae). The nymphs of P. bifoliata are gregarious in small groups and visited by three ant species (Crematogaster sp., Pheidole sp. and Technomyrmex sp.); however, the adults of P. bifoliata are solitary and are never observed with ants. Unlike other treehoppers that are subsocial, the females of P. bifoliata exhibited no maternal care behaviours, such as egg or nymph guarding.

What triggers phenological events in plants under seasonal environments? A study with phylogenetically related plant species in sympatry.

In this study, we evaluated variation in vegetative and reproductive phenological events of four phylogenetically related plant species subjected to a seasonal environment. To this aim, we sampled 15 individuals of each plant species every fortnight for one year, between January and December of 2017. To assess when a given phenophase occurred more intensely in the population, the Fournier intensity index was used and the synchrony of individuals of the sample in a given phenological event was estimated using the activity index. The Rayleigh (Z) test was used to determine whether the phenological events have seasonal distribution. The relationship of abiotic factors (photoperiod, precipitation, relative humidity and temperature) with the intensity of phenophases was evaluated for each plant species using generalized linear models (GLMs). The phenophases of all plants showed a seasonal distribution pattern, as well as variation in synchrony of phenophases and specific sets of abiotic factors significantly influenced their phenophases. New leaves, for example, were produced throughout the seasons, with intense leaf fall in the dry season. Flowering periods, on the other hand, did not overlap. Indeed, species exhibited sequential flowering and asynchronous flowering among individuals. Our results suggest that the phenological patterns of four sympatric plant species are directly linked to climatic variables, but different abiotic factors affected different phenophases.

Artificial Polyploidization Enhances Morphological, Physiological, and Biological Characteristics in Melothria scabra Naudin

Cucamelon (Meltric scabra Naudin, Cucurbitaceae) is a climbing, herbaceous perennial plant with various culinary and medicinal uses. The current study aimed to develop novel autopolyploid genotypes of M. scabra through in vitro polyploidization and assess their morphological and phytochemical characteristics. For polyploid induction, oryzalin was employed as an antimitotic agent, and it was applied at various concentrations (40, 60, and 80 μM) and durations (24 and 48 h). Flow cytometry analysis confirmed the successful induction of polyploids, with polyploidization efficiency ranging from 2.5% to 15%. From a total of 240 treated plants, a total of 20 autotetraploid plants were obtained. The obtained polyploid and control diploid genotypes were cultivated under greenhouse conditions. Further, the plants were transferred to field conditions, and the leaves, flowers, and fruits were harvested to be evaluated for the morphological, biochemical, and biological activity variations among the obtained genotypes. Morphological comparisons between diploid and autotetraploid plants revealed significant differences in flower characteristics, fruit attributes, and leaf morphology. Nutritional evaluation demonstrated enhancement of key parameters in the induced polyploids compared to the diploid plants, including glucose, fructose, carotenoid, polyphenol, and antioxidant contents, highlighting the potential impact of polyploidization on these traits. The results from this study highlight the potential of artificial chromosome doubling as an effective breeding strategy in M. scabra and related plant species.