One of the negative aspects of urbanization is the anthropogenic impacts and environmental conditions that alter the Habitat Integrity Index (HII) in urban water bodies. These facts can be studied and analyzed using the HII. The objective of this research was to generate data on the Paragominas stream from the C. do Sacramento highway and Residencial Selecta, in order to show the real state of these two environmental variables, in these two locations, which had not been available until now. The methodology applied was exploratory, observational, qualitative, and quantitative. The results obtained showed that in Area 1, the Constantino Pereira do Sacramento Highway, anthropogenic impacts (∑total = 55) and environmental conditions (∑total = 46) caused an average change in habitat integrity (∑total = 47.2%). In Area 2, Residencial Selecta, 518 m from PA 125, anthropogenic impacts (∑total = 31) caused changes in environmental conditions (∑total = 29), especially in land use and occupation with vegetation suppression, which significantly altered habitat integrity (∑total = 28.6%). Therefore, it can be concluded that urbanization and the construction of marginal residential areas on water bodies cause changes in the habitat of animals and plants, although it still maintains one of the four environmental services: bathing.