Geographical Distribution Of Plants Research Articles

Overexpression of CrSMT gene enhances salt stress tolerance by improving cotton peroxidation resistance

Salt stress is an important abiotic stress factor affecting crop production and plant geographical distribution. Salt stress negatively impacts molecular, biochemical, and physiological processes in cotton, resulting in inhibition of plant growth and development and, in severe cases, plant death. In this experiment, the CrSMT gene isolated from the unicellular eukaryote Chlamydomonas reinhardtii was overexpressed in cotton R15. Two transgenic lines, L17 and L25, were obtained. Treated with 200 mM NaCl experiments showed that the CrSMT-transgenic cotton had enhanced tolerance to salt stress. RNA-seq analysis revealed that CrSMT overexpression in cotton resulted in the synthesis of a large number of secondary metabolites responsive to salt stress. Correlation analysis between the wild type and the transgenic lines revealed that CrSMT overexpression did not affect the growth, agronomic traits, and fiber quality of cotton. The function of CrSMT holds potential to improve plant tolerance of abiotic stress factors.

Exploring Chemical Variability in the Essential Oils of the Thymus Genus.

Thyme remains an indispensable herb today, finding its place in gastronomy, medicine, cosmetics, and gardens worldwide. It is highly valued in herbal remedies and pharmaceutical formulations for its antibacterial, antifungal, and antioxidant properties derived from the richness of its essential oil, which comprises various volatile components. However, climate change poses a significant challenge today, potentially affecting the quality of thyme, particularly the extracted essential oil, along with other factors such as biotic influences and the plant's geographical distribution. Consequently, complex diversity in essential oil composition was observed, also influenced by genetic diversity within the same species, resulting in distinct chemotypes. Other factors contributing to this chemodiversity include the chosen agrotechnology and processing methods of thyme, the extraction of the essential oil, and storage conditions. In this review, we provide the latest findings on the factors contributing to the chemovariability of thyme essential oil.

Plant geographic distribution influences chemical defences in native and introduced Plantago lanceolata populations

Abstract Plants growing outside their native range may be confronted by new regimes of herbivory, but how this affects plant chemical defence profiles has rarely been studied. Using Plantago lanceolata as a model species, we investigated whether introduced populations show significant differences from native populations in several growth and chemical defence traits. Plantago lanceolata (ribwort plantain) is an herbaceous plant species native to Europe and Western Asia that has been introduced to numerous countries worldwide. We sampled seeds from nine native and 10 introduced populations that covered a broad geographic and environmental range and performed a greenhouse experiment, in which we infested half of the plants in each population with caterpillars of the generalist herbivore Spodoptera littoralis. We then measured size‐related and resource‐allocation traits as well as the levels of constitutive and induced chemical defence compounds in roots and shoots of P. lanceolata. When we considered the environmental characteristics of the site of origin, our results revealed that populations from introduced ranges were characterized by an increase in chemical defence compounds without compromising plant biomass. The concentrations of iridoid glycosides and verbascoside, the major anti‐herbivore defence compounds of P. lanceolata, were higher in introduced populations than in native populations. In addition, introduced populations exhibited greater rates of herbivore‐induced volatile organic compound emission and diversity, and similar chemical diversity based on untargeted analyses of leaf methanol extracts. In conclusion, the geographic origin of the populations had a significant influence on morphological and chemical plant traits, suggesting that P. lanceolata populations are not only adapted to different environments in their native range, but also in their introduced range. Read the free Plain Language Summary for this article on the Journal blog.

Genome-wide characterization of tobacco B-BOX gene family identified two close members play contrast roles under cold stress

Cold stress influences the growth and geographical distribution of plants. Tobacco (Nicotiana tabacum L.), a model in plant biology studies and a world-widely cultivated economic plant from tropical America, exhibits extreme sensitivity to low temperatures. The conserved B-BOX (BBX) transcription factors play vital roles in plant photomorphogenesis and flowering regulation, and their functions in abiotic stresses, including cold stress, have been uncovered recently. However, few studies on tobacco BBX genes have been conducted to date. In this study, 49 non-redundant BBX genes have been identified in the reference tobacco genome. Gene structure, phylogenetic relationship, prediction of cis-regulatory elements in promoters, tissue-specific expression patterns, and response to cold stress were conducted on these BBX gene members. The expression of NtBBX9 and NtBBX11, two close members in the amplified group II, was up-regulated under cold treatments. Overexpression of NtBBX11 embedded the cold tolerance of transgenic plants, while the NtBBX9 overexpressors were susceptible to cold. The NtBBX9 functions as a negative regulator of tobacco response to cold stress, which was confirmed by the phenotype of NtBBX9 RNA-silenced lines. Furthermore, we demonstrated that NtBBX9 and NtBBX11 regulated the cold stress response by alternating the expression of critical cold-regulated genes such as CBFs, LEA14, and LTI65 and reactive oxygen species (ROS) production. The combination of NtBBX9 and NtBBX11 could facilitate tobacco’s rapid response to and recovery from transient cold stress and regulate plant cold-responsive growth in tropical regions.

Madhuca longifolia var. latifolia (Roxb.) A.Chev: A plant with medicinal boon

Throughout human history, people used to obtain drugs from natural resources like plants to cure their ailments and improve their health. Nowadays human beings rely more on herbal medicines for the treatment of various ailments because they show better therapeutic activity and have very little to zero side effects on the human body. Madhuca longifolia is also one of the plants used in this category. The plant has been used by people for its medicinal properties since the Ayurveda period (before B.C 300). The plant possesses various medicinal activities including anti-proliferative, anti-inflammatory, anti-bacterial, anti-cancer, anti-ulcer and antidiabetic, and antioxidant activity. In various parts of India, the plant is used in the treatment of different diseases in ethnomedicinal practices. The plant possesses several phytochemicals which are responsible for its medicinal properties. The primary focus of this manuscript is to emphasize the significance of diverse ethnomedical characteristics, multiple preclinical investigations, and clinical trials conducted on this particular plant. The study also encompasses the plant's geographical distribution, pharmacognostic and anatomical studies, as well as its various anthropological applications within the Indian subcontinent.

The Influence of Macroclimatic Drivers on the Macrophyte Phylogenetic Diversity in South African Estuaries

The geographical distribution of plants is influenced by macroclimate and dispersal limitations, which have led to lineage isolation and subsequent diversification within and across various environmental gradients. Macroclimatic variables in coastal wetlands influence plant species and lineages across biogeographical boundaries. This study aimed to determine the influence of macroclimatic variables on species and phylogenetic richness in South African estuaries. Open-source chloroplast DNA barcoding sequences, species distribution and climatic data layers were used to determine the relationship between species richness, MPD, MNTD and each bioclimatic variable individually. Temperate species richness and phylogenetic diversity were positively correlated with temperature bioclimatic variables whereas subtropical and tropical species were associated with increases in precipitation. Phylogenetic niche conservatism is evident in malvids and rosids which are restricted to tropical and subtropical regions due to their physiological adaptations to tropical climates. Caryophylales was mostly associated with temperate regions. Poales and Alismatales showed wide distributions that is likely attributed to traits related to wind pollination and hydrochory, rapid, clonal, and high reproductive output, tolerance to stressful conditions, and intraspecific genetic diversity. The findings highlight the importance of considering macroclimate and phylogenetic factors in understanding the distribution and diversity of coastal wetland plants.

The role of ethylene in plant temperature stress response.

Temperature influences the seasonal growth and geographical distribution of plants. Heat or cold stress occur when temperatures exceed or fall below the physiological optimum ranges, resulting in detrimental and irreversible damage to plant growth, development, and yield. Ethylene is a gaseous phytohormone with an important role in plant development and multiple stress responses. Recent studies have shown that, in many plant species, both heat and cold stress affect ethylene biosynthesis and signaling pathways. In this review, we summarize recent advances in understanding the role of ethylene in plant temperature stress responses and its crosstalk with other phytohormones. We also discuss potential strategies and knowledge gaps that need to be adopted and filled to develop temperature stress-tolerant crops by optimizing ethylene response.

Population-Based Evidence of Climate Change Adaptation in an Endangered Plant Endemic to a Biodiversity Hotspot.

Climate change is expected to impact both the population structure and geographic distribution of plants. Species distribution models are widely used to assess range shifts and the vulnerability of plants to climate change. Despite the abundance of modeling studies, little is known about how existing populations respond to climate change. We investigated the demographic structure and vulnerability to climate change in Anemone moorei, a sub-shrub with a highly restricted distribution in a biodiversity hotspot. We improved the distribution knowledge through intensive field work. We conducted a census of stem length as a proxy for age for all known populations. We used ensemble forecasting to project distributions considering 10 future climate scenarios and developed a novel climate change vulnerability index for the species' distribution. We found that the mean stem length decreases and the proportion of young plants increases, while the size of fruiting plants decreases as A. moorei faces greater climate change vulnerability. We interpret these results as evidence for the onset of recent adaptation to climate change, consisting of reduced adult longevity and an earlier onset of reproduction. As a result of these changes, the proportion of juveniles in the population increases.

Arabidopsis LFR, a SWI/SNF complex component, interacts with ICE1 and activates ICE1 and CBF3 expression in cold acclimation.

Low temperatures restrict the growth and geographic distribution of plants, as well as crop yields. Appropriate transcriptional regulation is critical for cold acclimation in plants. In this study, we found that the mutation of Leaf and flower related (LFR), a component of SWI/SNF chromatin remodeling complex (CRC) important for transcriptional regulation in Arabidopsis (Arabidopsis thaliana), resulted in hypersensitivity to freezing stress in plants with or without cold acclimation, and this defect was successfully complemented by LFR. The expression levels of CBFs and COR genes in cold-treated lfr-1 mutant plants were lower than those in wild-type plants. Furthermore, LFR was found to interact directly with ICE1 in yeast and plants. Consistent with this, LFR was able to directly bind to the promoter region of CBF3, a direct target of ICE1. LFR was also able to bind to ICE1 chromatin and was required for ICE1 transcription. Together, these results demonstrate that LFR interacts directly with ICE1 and activates ICE1 and CBF3 gene expression in response to cold stress. Our work enhances our understanding of the epigenetic regulation of cold responses in plants.

Integration of chromatin accessibility and gene expression reveals new regulators of cold hardening to enhance freezing tolerance in Prunus mume.

Low temperature is one of the most important abiotic factors limiting the growth, development and geographical distribution of plants. Prunus mume is an attractive woody ornamental plant that blooms in early spring in Beijing. However, the molecular mechanisms underlying cold hardening to enhance freezing tolerance in Prunus genus remains elusive. This study examined the dynamic physiological responses induced by cold hardening, and identified freezing-tolerance genes by RNA-seq and ATAC-seq analyses. Cold hardening elevated the content of soluble substances and enhanced freezing resistance in P. mume. Transcriptome analysis indicated that the candidate differentially expressed genes (DEGs) were those enriched in Ca2+ signalling, mitogen-activated protein kinase (MAPK) cascade, abscisic acid signalling, and inducer of CBF expression 1 (ICE)-C-repeat binding factor (CBF) signalling pathways. The openness of gene chromatin positively correlated with the expression level of these genes. Thirteen motifs were identified in the open chromatin regions in the treatment group subjected to freezing after cold hardening. The chromatin opening of transcription start site at the proximal -177 region of cold-shock protein CS120-like (PmCSL) was markedly increased, while the expression level of PmCSL was significantly up-regulated. Overexpression of PmCSL in Arabidopsis significantly improved the freezing tolerance of transgenic plants. These findings provide new insights into the regulatory mechanism of freezing tolerance to improve breeding of cold-hardy P. mume plants.

The use of plant (Citrullus colocynthis) in alternative medicine

Alternative medicine is one of the most important choices for humans, as many people are going to live closer to nature, because they have seen good results for most herbs that replace the chemical drugs that have greatly affected the human body. The research investigates the historical facts of alternative medicine, the use of Citrullus colocynthis, the treatment of many diseases, the geographical distribution of plants in Iraq, and the proposal to invest in plants in Iraq.

Physicochemical characteristics of fresh and lyophilized Georgian royal jelly and Formulation bentonite-based cream

Royal Jelly (RJ) is one of the most valued and studied bee products used in medicine and cosmetics due to its diverse pharmacological properties. Chemical composition and biological activity of RJ depends on many factors, including constitution and geographical ​​distribution of plants, the harvest time and bee species. The objective of the present study was physico-chemical characterization and comparison of fresh and lyophilized RJ; and development dermatological formulation by incorporating RJ in Tikha Ascane base. The evaluations of RJ and RJ loaded formulations were performed by oven-dry, titrimetric, potentiometric, FTIR methods; 10-hydroxy-2-decenoic acid (10-HDA) was determined by high-performance liquid chromatography. In addition, the physical stability of the developed formulations was investigated by examining viscosity, spreadability, thermal and colloidal stabilities, pH, homogeneity and appearance. Lyophilized RJ displayed up to similar physico-chemical properties (solubility, pH, acidic number) as fresh RJ. The chromatographic analysis showed alike fingerprints and the existence of 10-HAD in tested samples. No spectral differences (in the range of 3600–2800 cm-1 and 1750–950 cm-1) were observed in FTIR measurements after lyophilization. The study shows that prepared formulations were found to be stable. The tested samples had appealing appearance with smooth texture and no signs of separation. Viscosity, homogeneity, spreadability, pH and release profile of RJ from the formulations were acceptable.

Seed traits and phylogeny explain plants' geographic distribution

Abstract. Understanding the mechanisms that shape the geographic distribution of plant species is a central theme of biogeography. Although seed mass, seed dispersal mode and phylogeny have long been suspected to affect species distribution, the link between the sources of variation in these attributes and their effects on the distribution of seed plants are poorly documented. This study aims to quantify the joint effects of key seed traits and phylogeny on species distribution. We collected the seed mass and seed dispersal mode from 1426 species of seed plants representing 501 genera of 122 families and used 4 138 851 specimens to model species distributional range size. Phylogenetic generalized least-squares regression and variation partitioning were performed to estimate the effects of seed mass, seed dispersal mode and phylogeny on species distribution. We found that species distributional range size was significantly constrained by phylogeny. Seed mass and its intraspecific variation were also important in limiting species distribution, but their effects were different among species with different dispersal modes. Variation partitioning revealed that seed mass, seed mass variability, seed dispersal mode and phylogeny together explained 46.82 % of the variance in species range size. Although seed traits are not typically used to model the geographic distributions of seed plants, our study provides direct evidence showing seed mass, seed dispersal mode and phylogeny are important in explaining species geographic distribution. This finding underscores the necessity to include seed traits and the phylogenetic history of species in climate-based niche models for predicting the response of plant geographic distribution to climate change.

Screening for insecticidal toxicity of Urginea maritima (L.) bulbs and Asphodelus microcarpus (L.) tubers for the control of Tribolium castaneum (Tenebrionidae)

ABSTRACT This research was conducted to determine the effect of geographical distribution on morphological characteristics of plants and some chemical compounds such as polyphenols. Moreover, we explore the contact toxicity of ethanolic and methanolic extracts from Urginea maritima bulbs and Asphodelus microcarpus tubers collected from three localities in the North West of Tunisia against Tribolium castaneum adults. The toxicity results demonstrated that both of the studied plant extracts had an effective control against T. castaneum at concentration 7.5 µL/L air, thus total mortality reached. In addition, results showed plant extracts with methanol exhibited high mortality percentage of T. castaneum. On the other hand, results revealed that chemical composition of U. maritime bulbs and A. microcarpus tuber extract depends on the soil components and geographical distribution of plants. The present investigation confirmed that A. microcarpus and U. maritima may be recommended as an eco-friendly alternative to synthetic insecticide against T. castaneum.

ICE-CBF-COR Signaling Cascade and Its Regulation in Plants Responding to Cold Stress.

Cold stress limits plant geographical distribution and influences plant growth, development, and yields. Plants as sessile organisms have evolved complex biochemical and physiological mechanisms to adapt to cold stress. These mechanisms are regulated by a series of transcription factors and proteins for efficient cold stress acclimation. It has been established that the ICE-CBF-COR signaling pathway in plants regulates how plants acclimatize to cold stress. Cold stress is perceived by receptor proteins, triggering signal transduction, and Inducer of CBF Expression (ICE) genes are activated and regulated, consequently upregulating the transcription and expression of the C-repeat Binding Factor (CBF) genes. The CBF protein binds to the C-repeat/Dehydration Responsive Element (CRT/DRE), a homeopathic element of the Cold Regulated genes (COR gene) promoter, activating their transcription. Transcriptional regulations and post-translational modifications regulate and modify these entities at different response levels by altering their expression or activities in the signaling cascade. These activities then lead to efficient cold stress tolerance. This paper contains a concise summary of the ICE-CBF-COR pathway elucidating on the cross interconnections with other repressors, inhibitors, and activators to induce cold stress acclimation in plants.

Genome Size of Life Forms of Araceae-A New Piece in the C-Value Puzzle.

The genome size of an organism is an important trait that has predictive values applicable to various scientific fields, including ecology. The main source of plant C-values is the Plant DNA C-values database of the Royal Botanic Gardens Kew, which currently contains 12,273 estimates. However, it covers only 2.9% of known angiosperm species and has gaps in the life form and geographic distribution of plants. Only 4.5% of C-value estimates come from researchers in Central and South America. This study provides 41 new C-values for the aroid family (Araceae), collected in the Piedras Blancas National Park area in southern Costa Rica, including terrestrial, epiphytic and aquatic life forms. Data from our study are combined with C-value entries in the RBGK database for Araceae. The analysis reveals a wider range of C-values for terrestrial aroids, consistent with other terrestrial plants, a trend toward slightly lower C-values for epiphytic forms, which is more consistent for obligate epiphytes, and comparatively low C-values for aquatic aroids.

More than the usual suspect: diversity of pollinators of chayote (Sechium edule) at high elevations in Chiapas, Mexico

Crops that offer multiple types of value, for example, in terms of economy, culture, and health, frequently lack information on the basic ecological interactions that play a significant role in their persistence. Chayote (Sechium edule, Cucurbitaceae), a widespread squash valued for its nutritious fruit, is one example. We aimed at describing the assemblage of flower visitors to this crop at 2400 m where stingless bees, reported as the main pollinators, are naturally absent. We implemented flower exclusions, performed field observations, and inspection of pollen loads of captured visitors to be able to draw distinctions between primary and secondary pollinators. We recorded a total of 60 species or morphospecies of insects visiting the flowers. Bees, wasps, and flies, present in all sites and consistently carrying abundant pollen, are the main pollinators of S. edule at high altitudes. Our study adds to the building evidence of the fundamental role that native bees and wasps play as crop pollinators in subsistence farming. Basic ecological knowledge is essential to inform agricultural management policies and to foresee preventable food scarcity problems, especially in view of climate change scenarios that predict drastic alterations in plant geographical distributions.

What if the cold days return? Epigenetic mechanisms in plants to cold tolerance.

The epigenetic could be an important, but seldom assessed, mechanisms in plants inhabiting cold ecosystems. Thus, this review could help to fill a gap in the current literature. Low temperatures are one of the most critical environmental conditions that negatively affect the growth, development, and geographic distribution of plants. Exposure to low temperatures results in a suit of physiological, biochemical and molecular modifications through the reprogramming of the expression of genes and transcription factors. Scientific evidence shows that the average annual temperature has increased in recent years worldwide, with cold ecosystems (polar and high mountain) being among the most sensitive to these changes. However, scientific evidence also indicates that there would be specific events of low temperatures, due it is highly relevant to know the capacity for adaptation, regulation and epigenetic memory in the face of these events, by plants. Epigenetic regulation has been described to play an important role in the face of environmental stimuli, especially in response to abiotic stress. Several studies on epigenetic mechanisms have focused on responses to stress as drought and/or salinity; however, there is a gap in the current literature considering those related to low temperatures. In this review, we focus on systematizing the information published to date, related to the regulation of epigenetic mechanisms such as DNA methylation, histone modification, and non-coding RNA-dependent silencing mechanisms, in the face of plant´s stress due to low temperatures. Finally, we present a schematic model about the potential responses by plants taking in count their epigenetic memory; considering a global warming scenario and with the presence or absence of extreme specific events of low temperatures.

Bermudagrass CdWRKY50 gene negatively regulates plants’ response to salt stress

Salinity influences the growth and geographical distribution of plants. Bermudagrass [Cynodon dactylon (L). Pers.] shows relatively strong tolerance to salt, and hence contributes to restoration of saline-alkali land. WRKY proteins have been proven to be widely involved in regulating salt stress response in plants. However, to date, the functions of bermudagrass WRKY genes that are involved in salinity response remain largely unknown. In this study, CdWRKY50 was isolated and analyzed in bermudagrass. The expression of CdWRKY50 was up-regulated by salt, drought, cold, and abscisic acid treatments. Silencing CdWRKY50 via a virus-induced gene silencing method enhanced salt tolerance of bermudagrass. Conversely, transgenic Arabidopsis overexpressing CdWRKY50 lines showed salt-sensitive phenotype compared with wild type. Furthermore, CdWRKY50 can bind to AtDREB2Apromoter and regulate its expression by yeast one-hybrid and dual-luciferase reporter assay, thus regulating its expression. Taken together, our study revealed that CdWRKY50 is a negative regulator mediating plants response to salt stress, which provides scientific foundation for developing salt-tolerant plants through genetic engineering.

Physiological and transcriptome analysis of Poa pratensis var. anceps cv. Qinghai in response to cold stress

BackgroundLow temperature limits the growth and development and geographical distribution of plants. Poa pratensis is a cool-season turfgrass mainly grown in urban areas. However, low winter temperature or cold events in spring and autumn may cause P.pratensis mortality, affecting the appearance of lawns. P.pratensis var. anceps cv. Qinghai (PQ) is widely distributed in the Qinghai-Tibet Plateau above 3000 m. PQ has greater cold tolerance than the commercially cultivated P.pratensis varieties. However, existing studies on the response mechanism of PQ to low temperatures have mainly focused on physiological and biochemical perspectives, while changes in the PQ transcriptome during the response to cold stress have not been reported.ResultsTo investigate the molecular mechanism of the PQ cold response and identify genes to improve the low-temperature tolerance of P.pratensis, we analyzed and compared the transcriptomes of PQ and the cold-sensitive P.pratensis cv. ‘Baron’ (PB) under cold stress using RNA sequencing. We identified 5996 and 3285 differentially expressed genes (DEGs) between the treatment vs control comparison of PQ and PB, respectively, with 5612 DEGs specific to PQ. Based on the DEGs, important Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, such as “starch and sucrose metabolism”, “protein processing in endoplasmic reticulum”, “phenylalanine metabolism” and “glycolysis/gluconeogenesis” were significantly enriched in PQ, and “starch and sucrose metabolism”, “phenylpropanoid biosynthesis”, “galactose metabolism” and “glutathione metabolism” were significantly enriched in PB. In addition, the “glycolysis” and “citrate cycle (TCA cycle)” pathways were identified as involved in cold tolerance of P.pratensis.ConclusionsAs we know, this is the first study to explore the transcriptome of P.pratensis var. anceps cv. Qinghai. Our study not noly provides important insights into the molecular mechanisms of P.pratensis var. anceps cv. Qinghai responds to cold stress, but also systematically reveals the changes of key genes and products of glycolysis and TCA cycle in response to cold stress, which is conductive to the breeding of cold-tolerance P.pratensis genotype.