Plant-based Meat Research Articles

Substitution patterns and price response for plant-based meat alternatives

Efforts to promote sustainable resource use through reduced meat consumption face challenges as global meat consumption persists. The resistance may be attributed to the lower sales price of meat compared to most plant-based meat alternatives (PBMAs). Addressing this, our research delves into the pivotal question of which PBMAs resonate most with consumers and how pricing affects demand. In a hypothetical restaurant context, we conducted 2 representative studies among 2,126 individuals in the United States to scrutinize preferences for meat, analog, semi-analog, and non-analog burgers. First, in a survey, we assessed rankings of the four burgers, alongside evaluating participants’ genuine consideration of these choices to discern a diverse preference distribution. Subsequently, in an experiment, we examined the influence of prices on participants’ consideration and choice of PBMAs, thereby capturing both phases of the decision-making process. Our survey shows that meat has considerably higher utility and consumer preference than all PBMAs on average, but we also find substantial heterogeneity (i.e., some consumers prefer PBMAs over meat). In the experiment, we establish that there is a negative association between the consideration of meat and PBMA burgers, though consideration of any one PBMA is positively associated with considering other PBMAs. A noteworthy increase in consideration and choice is observed when prices of PBMAs are reduced, while changing the price of the meat burger only has minimal effect on demand. Such findings underscore the importance of affordability beyond price parity in catalyzing the shift toward plant-based diets.

A perspective on the environmental impact of plant-based protein concentrates and isolates

Plant-based proteins are promoted as nutritious, environmentally safe, and a key element for food security. Numerous studies using environmental life cycle assessments confirmed plant-based meat alternatives are more environmentally favorable than animal-derived meat. However, most missed other factors from protein extraction and concentration that contribute to environmental impact (both positive and negative). Furthermore, few studies examined the entire environmental impact because they overlooked social and nutritional life cycle assessments. Many publications assumed most environmental impact occurred in crop cultivation before high protein products are manufactured. However, industrial plant-based protein extraction, concentration, and purification require large amounts of natural resources, energy, chemicals, and highly specialized equipment seldom accurately assessed from an environmental, social, and nutritional perspective. This paper presents opinions and perspectives on additional information to consider to fully realize environmental benefits of highly proteinaceous plant-based food ingredients. Elements are also discussed we perceive as “hot spots” for future assessments in presenting a complete picture for minimizing environmental impact and how the numerous actors in this supply chain can transform our food system and secure protein availability for future generations.

Sentiments, barriers and predictors of acceptance for meat alternatives among UK meat consumers

There is a growing global demand for diets to be better for the environment and for human health. To support the shift toward sustainable diets, it is crucial to understand consumer attitudes and the barriers they face. To achieve this aim, this paper addresses the questions: What are the prevailing sentiments of UK meat consumers towards meat alternatives? What barriers do UK meat consumers face in trying and sustaining the consumption of meat alternatives? Data was obtained from 1074 UK respondents. The majority of respondents (82.4%) had tried plant-based meat alternatives, whereas most had not tried edible insects (88.8%), cultured meat (96.5%) or algae (73.7%). Sentiments towards plant-based alternatives were mainly positive while views on cultured meat, insects, and algae were more mixed or negative. Motivations for accepting and eating meat alternatives were primarily personal health benefits, animal welfare and environmental reasons in order of ranking. The results show that 18% are very likely to include meat alternatives in their future diets, followed by 39% who are open to the possibility and may consider it under the right circumstances. Ordered logistic regression identified several significant predictors for incorporating meat alternatives into future diets. Positive predictors included price competitiveness, variety, environmental motivation, health benefits, past experience with plant-based meat and algae and gender. Conversely, frequent meat consumption and safety concerns were negative predictors and reduced the odds of incorporating meat alternatives into diets. Key actions which consumers opine would encourage acceptance of meat alternatives includes educational programs, financial incentives and media campaigns with strong support for coordinated stakeholder efforts.

Plant-based Meat Analogs: Perspectives on Their Meatiness, Nutritional Profile, Environmental Sustainability, Acceptance and Challenges.

Plant-based meat analogs (PBMAs) have been the subject of interest over the past few years due to consumers' demand for environmentally friendly, healthful, and non-animal-based foods. A better comprehension of the composition, structure, texture, nutrition, and sustainability of these PBMAs is necessary. This review articulates the protein sources and composition of PBMAs and their "meatiness" with respect to texture, structure, and flavor enhancement. The components used in the analogs, such as unsaturated fats, fibers, vitamins, minerals, carbohydrates, and plant-based oils enriching their nutritional profile, are described. The study identifies the environmental and sustainability impact of PBMAs, as crucial to the survival and maintenance of biodiversity. More studies are warranted to scope and underscore the significance of the analogs and comprehend the texture or structure-function relationships. Further product development and testing thereof may ultimately result in quality meat analogs that respect meat taste, health and acceptance of consumers, environmental sustainability, animal welfare, and current challenges.

Emulsion gels with chia flour enhance quality of plant-based meat analogs by reducing fat and improving texture

Vegetable or coconut oils may negatively impact the texture and lipid profile of plant-based meat analogs. This study aimed to develop emulsion gels (EGs) using olive oil, combined with oat bran, chia flour, and soy protein isolate, to enhance the texture, lipid profile, and overall quality of plant-based patties (PPs). Six EGs were formulated with 40% olive oil and 15 or 20% chia flour, oat bran, or soy protein isolate: chia flour EG (CEG15 and CEG20), oat bran EG (OEG15 and OEG20), and soy protein isolate EG (SEG15 and SEG20). The physicochemical properties of EGs and the physicochemical and sensory properties of the EG-supplemented PPs were examined. The oil-binding capacity was higher in CEG15 and CEG20 followed by SEG15 and SEG20. The overall viscosity was higher in CEG20 and SEG20. All EG-supplemented PP groups showed significantly lower cooking loss, lipid oxidation, and increased water-holding capacity. Particularly, the PP-CEG15 and PP-CEG20 groups exhibited lower exudation rates and higher hardness in the texture analysis than the other groups. Additionally, the PP-CEG groups had desirable sensory scores compared to the other groups. In conclusion, incorporating EGs with different plant-based ingredients, especially chia flour, can enhance the lipid profile and overall quality of PPs.

Promoting artificial meat to improve food security and reduce resource-environment pressure: is it practicable in China?

Artificial meat is increasingly recognized as a crucial innovation for addressing global food security challenges and reducing environmental pressures. This study aims to understand the practicability of promoting artificial meat consumption to achieve the dual goals of improving food security and reducing resource-environment pressure by evaluating Chinese consumers’ willingness to pay (WTP) for artificial meat. A discrete choice experiment was conducted via an online survey involving 998 consumers from five representative Chinese cities. The experiment was designed to elicit preferences and WTP for artificial meat. A random information intervention providing positive information about artificial meat was included to examine its impact on consumer choices. The estimation results indicate significantly lower WTP for both cultured and plant-based meat compared to farm-raised meat, with plant-based meat receiving higher WTP than cultured meat. The information intervention notably reduced the WTP gap between artificial meat and farm-raised meat, suggesting that consumer education can positively influence acceptance. The findings suggest that the market introduction of artificial meat in China faces considerable challenges, particularly regarding consumer acceptance and perceived value. While positive information can reduce the disparity in WTP, the practicability of promoting artificial meat to achieve food security and environmental sustainability goals is currently limited. This study contributes to the literature on consumer attitudes towards artificial meat in China and provides critical insights for policymakers aiming to promote the consumption of artificial meat.

Risk ranking of mycotoxins in plant-based meat and dairy alternatives under protein transition scenarios

While reducing the consumption of animal-source foods is recommended for planetary and human health, potential emerging food safety risks associated with the transition to dietary patterns featuring plant-based meat (PBMA) and dairy alternatives (PBDA) remain unexplored. We assessed the exposure to mycotoxins and ranked the associated health risks related to the consumption of PBMA and PBDA. We simulated diets by replacing animal-source proteins with their plant-based alternatives. A risk ratio method, based on the hazard quotient (HQ), was applied to rank mycotoxin-related food safety risks. An aggregated dataset containing contamination data of 45 mycotoxins distributed over 182 PBDA and 131 PBMA samples, collected and analyzed in Europe, representing a convenience sample, was used as input in our risk assessment. The highest risk mycotoxins identified for PBMA in the lower bound scenario (LB) were the sum of aflatoxins (sum AF), alternariol monomethyl ether (AME), aflatoxin B1 (AFB1) and alternariol (AOH), with HQs for children of 121.3, 66.8, 24.0 and 4.3, respectively. In the milk substitution model (LB), HQs of 4.9 (sum AF) and 1.4 (AFB1) were calculated for children, indicating a potential food safety risk. Further analysis demonstrated that soy-based meat alternatives are the highest risk food products, in particular for sum AF, AME, AOH and AFB1. With regard to PBDA, highest HQs were found for sum AF in almond and oat drink, and AFB1 in oat drink. This research presents an approach to overcome the lack of data in the assessment of emerging risks associated with the shift to more sustainable dietary patterns.

How do push and pull factors affect non-vegetarians’ intention to buy plant-based meat products?

PurposeThis study aims to analyze the push and pull factors and its relationship with the theory of planned behavior about non-vegetarians’ intentions to buy plant-based meat products. Previous studies seldom explored the intention of non-vegetarians’ intention to buy plant-based meat products.Design/methodology/approachAn online survey was conducted among 447 non-vegetarians to investigate these relationships. Partial least squares-structural equation modeling (PLS-SEM) was employed to scrutinize the data.FindingsAmong the push factors product safety and flavor positively influence consumers’ attitude, whereas environmental protection and flavor positively influence subjective norms. Animal welfare, environmental protection and flavor positively influence perceived behavioral control. Among the pull factors, curiosity, product nutrition and price positively influence consumer attitudes toward plant-based meat products. Curiosity and price also positively influence subjective norms, but only product nutrition positively influences perceived behavioral control. On the other hand, the results also confirm that non-vegetarian customers' intentions to buy plant-based meat products is primarily influenced by attitude and perceived behavioral control, whereas subjective norms do not influence the intention to buy plant-based meat products. This study also reveals that there exists a moderating influence from perceived behavioral control between attitude and intention to buy plant-based meat products, whereas the moderating effect of perceived behavioral control on subjective norms and intention is insignificant.Originality/valueThe findings of this study offer practical recommendations for persuasive marketing strategies associated with plant-based meat substitutes.

Plant-Based Meat Alternatives Predicted by Theory of Planned Behavior Among Midwest Undergraduates

Plant-based meat alternatives (PBMAs) such as the Impossible Burger® imitate animal meat appearance, taste, feel, and texture. Part of their consumer appeal are the views that PBMAs are more environmentally friendly, reduce inhumane treatment of animals, and/or have preferred nutritional attributes. College-educated adults are one of the larger markets for these products. This cross-sectional online survey utilized the Theory of Planned Behavior to predict self-reported intakes of PBMAs among 536 undergraduates aged 18–25 at a Midwest university. Sixty-one percent had eaten PBMAs, and 17% wanted to try them. Twenty-two percent were uninterested non-consumers. Their top reason for not eating PBMAs was that they had no reason to decrease their meat intake. Multinomial logistic regression analysis showed subjective norms and positive attitudes about PBMAs increased the odds of more frequent intake, whereas non-consumers had less support from social contacts, but greater perceived behavioral control over general food access. Thus, those with supportive social influences, concerns about the environment, and animal welfare are more likely to consume PBMAs. More frequent PBMA consumption was observed among U.S.-born multicultural students, food insecure students, and those with less perceived behavioral control over food access. Future research should investigate the nuances between these associations further by examining the types of PBMAs consumed, their costs, and retail sources across student demographics.

Plant Taxa as Raw Material in Plant-Based Meat Analogues (PBMAs)—A Patent Survey

Background/Objectives: The environmental problems associated with meat production, the pain and distress of animals, and health problems have contributed to the increased appreciation of meat alternatives. Methods: The review of patented inventions presenting plant-based meat analogues (PBMAs)issued in the years 2014-2023 was conducted according to PRISMA statements across the ISI Web of Science, as well as Google Patents and Espacenet Patent Search engines. Results: The analysis of 183 patents showed an increase inpatent numbers in the years 2020–2022, with the greatest number of patents developed by teams consisting of two authors. The authors and patent applicants were mainly affiliated with the United States, while Société des Produits Nestlé S.A. emerged as the leader among applicant institutions. The International Patent Classification (IPC) codes were given to 177 patents, which were mainly classified as Human Necessities (Section A). In total, inventors mentioned 184 taxa, including 28 genera, 1 section, 144 species, 5 subspecies and 6 varieties of vascular plants. The majority of taxa showed a high edibility rating and belonged to perennials, mainly herbaceous plants representing the families Fabaceae, Poaceae and Brassicaceae. The analysis of patents showed that plants are a promising source of protein, lipids, fibre, polyphenols, starch and gum in meat analogues. At the same time, the noticed slight use of numerous taxa, despite the substantial content of valuable constituents as well as high edibility rates, presumably might be caused by their underutilization in numerous regions of the world. Conclusions: The direction of future studies should focus on searching for novel plant-based meat analogues based on underutilized, promising plant sources and investigations of their usefulness.

Influence of selected ingredients and process variables on quality parameters of saturated steam-assisted plant-based meat analogue

Purpose Plant-based meat analogues (PBMAs) hold significant promise as a sustainable solution to meet future protein demands, replicating the taste and nutritional value of meat. However, the present reliance on extrusion technology in PBMA production limits the exploration of more accessible and affordable methods. The current investigation aims to meet the market demand for a scalable and cost-effective processing approach by exploring saturated steam-assisted technology that could broaden the production volume of PBMAs, thereby supplementing protein security and planet sustainability. Design/methodology/approach A one-factor-at-a-time (OFAT) approach is employed to evaluate the effect of ingredients and process conditions on the governing quality attributes (texture, colour and sensory). Findings Among the ingredients, monosodium glutamate (MSG) and nutritional yeast (NY) significantly enhanced the hardness and chewiness of saturated steam-assisted plant-based meat analogues (ssPBMAs) followed by potato protein isolate (PPI), defatted soy flour (DSF) and salt. The addition of PPI and DSF led to a decrease in lightness (L* value) and an increase in the browning index (BI). Sensory evaluations revealed that higher concentrations of DSF imparted a noticeable beany flavour (>20%), whereas PPI (30%) improved the overall sensory appeal. Increased levels of NY (10%) and MSG (5%) enhanced the umami flavour, enhancing consumer preference. Higher thermal exposure time (TTi) (45 min) and temperature (TTe) (120 °C) during processing resulted in softer products with reduced L* values. These findings establish a foundation for selecting and optimizing the ingredients and processing parameters in ssPBMA production. Originality/value The novelty of the current study includes process behaviour of selected ingredients such as PPI, NY, MSG, DSF, salt and adopted process conditions, namely, dough processing time (DPT), protein network development time (PNDT), TTi and TTe on the quality of ssPBMAs.

Sensory and hedonic perception of meat versus ultra-processed plant-based meat analogs: A comparative study.

Nowadays, consumption habits are changing for different reasons, and animal products are being replaced by plant-based analogs. Some of these analogs are classified as ultra-processed foods (UPFs) according to NOVA criteria (food classification system). Within this scenario, little is known about consumers' sensory response regarding these new products. One main challenge of plant-based UPFs is imitating both the sensory characteristics and the hedonic experience of meat consumption. Although numerous studies explore the sensory profile of ultra-processed vegan products, we found no comparisons between the sensory profiles of plant-based analogs and their corresponding meat products. The present study aimed to compare the dynamic sensory profiles, specifically focusing on flavor and texture attributes, of animal-based food (beef and chicken meat) and their corresponding commercial ultra-processed plant-based meat analogs (seitan and Mediterranean chick'n pieces) and to establish the adequacy of the intensity of certain sensory characteristics (attributes) and their impact on the acceptability of meat analogs. This was conducted using the time-intensity technique with fourteen trained panelists and just about right scales with 59 consumers, evaluating the acceptability of the products with a 7-point hedonic scale. The dynamic perception results for the analogs significantly differed from those of beef and chicken, specifically showing higher intensity in "nonmeat" attributes such as gumminess, mushroom flavor, vegetable flavor, and spiciness. The attributes that characterize the chicken analog are more suitable than the attributes that characterize seitan. The acceptability of seitan was not significantly influenced by the attribute suitability results. However, poor juiciness showed a significant penalty on its acceptance for the chicken analog. These identified differences highlight the need to evaluate and adjust the sensory characteristics of plant-based products that attempt to imitate traditional meat products. PRACTICAL APPLICATION: The time-intensity technique was effective in identifying differences in the consumption experience between meat and the respective plant-based analogs. Inadequate taste and texture characteristics can decrease the acceptability of the analogs. The identification of these characteristics is crucial for the food industry to understand consumer preferences regarding meat substitutes.

Near Infrared Reflectance Spectroscopy-Driven Chemometric Modeling for Predicting Key Quality Traits in Lablab Bean (Lablab purpureus L.) Germplasm

Lablab bean (Lablab purpureus L.) is a multipurpose crop, commonly used for food, feed, and fodder, and its potential as a plant-based meat alternative. Its nutritional diversity, including high protein, starch, and phenolic content, makes it a suitable candidate for nutritional profiling, which is essential for developing nutritionally enhanced varieties. Traditional methods for analyzing its nutritional parameters are labor-intensive, time-consuming, and expensive. This study employs Near-Infrared Reflectance Spectroscopy (NIRS) as a rapid, non-destructive alternative to evaluate 112 Lablab bean genotypes. We developed prediction models for starch, amylose, protein, fat, and phenols using a Modified Partial Least Squares (MPLS) approach, with spectral pre-processing using Standard Normal Variate (SNV) to remove scatter effects and Detrending (DT) to reduce baseline shifts and noise. The models were optimized for derivatives, gap selection, and smoothing, and evaluated using independent test data and key performance metrics including coefficient of determination (R²), bias, and Residual Prediction Deviation (RPD). The best-performing models were: starch (R² = 0.959, RPD = 4.57), amylose (R² = 0.737, RPD = 1.76), protein (R² = 0.911, RPD = 3.09), fat (R² = 0.894, RPD = 2.92), and phenols (R² = 0.816, RPD = 2.36). Statistical tests, including paired t-tests, correlation, and reliability analysis, confirmed the robustness of these models. This study presents a first report offering rapid, multi-trait assessment method for evaluating Lablab bean germplasm, demonstrating high predictive accuracy for pre-breeding practices. It has broad applications in developing nutritionally enhanced varieties, supporting plant-based protein alternatives, and optimizing food production processes to meet the growing demand for healthier, sustainable foods.

Plant-based mince texture: A review of the sensory literature with view to informing new product development.

The texture of plant-based meat alternatives is a sensory attribute that holds a central value in consumer acceptability. The texture dimensions and drivers of liking for plant-based mince, a popular product within plant-based meats, are not yet fully established and literature in this area is limited. Consequently, the successful development and positioning of such products in the market next to traditional proteins is compromised, and improved product development guidelines are needed for the industry. This review aims to inform product development of what is currently known about plant-based mince texture and where the research gaps are, particularly with respect to sensory dimensions and drivers of liking, by reviewing the present landscape of relevant sensory literature. Potential texture sensory dimensions for plant-based mince, based on limited studies and seemingly aligning with conventional mince, are posited as juiciness, tenderness, firmness, and/or softness with a key driver of liking being juiciness, but this requires further validation utilizing robust sensory studies. A significant need exists to expand on the currently known texture dimensions and drivers alongside new ways that texture can be improved upon to more closely align to, or exceed, consumer expectations. Once this has been achieved, a robust sensory framework for developing plant-based mince products can be used to better position plant-based mince to effectively compete with traditional mince, and not merely cannibalize upon other brands, in the current market.

The mechanical and sensory signature of plant-based and animal meat

Eating less meat is associated with a healthier body and planet. Yet, we remain reluctant to switch to a plant-based diet, largely due to the sensory experience of plant-based meat. Food scientists characterize meat using a double compression test, which only probes one-dimensional behavior. Here we use tension, compression, and shear tests–combined with constitutive neural networks–to automatically discover the behavior of eight plant-based and animal meats across the entire three-dimensional spectrum. We find that plant-based sausage and hotdog, with stiffnesses of 95.9 ± 14.1 kPa and 38.7 ± 3.0 kPa, successfully mimic their animal counterparts, with 63.5 ± 45.7 kPa and 44.3 ± 13.2 kPa, while tofurky is twice as stiff, and tofu is twice as soft. Strikingly, a complementary food tasting survey produces in nearly identical stiffness rankings for all eight products (ρ = 0.833, p = 0.015). Probing the fully three-dimensional signature of meats is critical to understand subtle differences in texture that may result in a different perception of taste. Our data and code are freely available at https://github.com/LivingMatterLab/CANN

Current Strategies to Modify the Functional Properties of Proteins Extracted from Pumpkin Seeds: A Comprehensive Review

The functional properties of pumpkin seed proteins remain unutilized in numerous food and industrial applications. Several current approaches aim to improve the functional properties of pumpkin seed proteins, allowing their innovative potential to develop and modify significantly. Several strategies can be implemented to alter the functional properties of proteins isolated from pumpkin seeds. The first is enzymatic hydrolysis, regardless of whether, proteases may free peptide binding and profoundly impact the protein structure and functionality. Thermal treatment can include heating and cooling to replace protein conformation and increase solubility, emulsification, and gelation properties. Chemical modification techniques, including acylation and glycation, can also be used to improve stability, viscosity, and foaming ability. Functional properties and, where possible, ingredients with many applications may include exceptional possibilities for proteins modified in food preparations, such as dairy replacements, plant-based meat analogues, and free gluten that have an outstanding aspect, satisfactory quality, and nutritional profiles. As multiple different proteins act as precursors of active peptides, they can also be used to generate bio-specific foods. This review briefly provides information about various types of protein extraction techniques and functional properties that are modified by different types of processing technologies.

Microbiological Quality of Plant-based Meat-alternative Products Collected at Retail Level in Switzerland

As the interest in plant-based alternative food products has increased significantly in the last years, it is also important to investigate these products regarding microbiological aspects. The aim of this study was to assess the microbiological quality and the occurrence of selected foodborne pathogens in plant-based meat alternative products (PBMA) collected at retail level in Switzerland. A total of 100 PBMA (84 vegan and 16 vegetarian products) was analyzed qualitatively for the presence of Salmonella, Listeria monocytogenes and quantitatively for Staphylococcus aureus, Bacillus cereus group members, Enterobacteriaceae, and the total viable count. Furthermore, pH measurements were carried out and the aw-value was determined. Isolates were further analyzed with Whole Genome Sequencing. The total viable count before the expiration date was between <2 log and 7 log CFU/g (median: 5.97 log CFU/g). In six (6%) samples, Enterobacteriaceae with 2 log to 3 log CFU/g were detected. No Salmonella and no Listeria monocytogenes were detected. However, seven products (7%) were contaminated with other Listeria spp. (six L. innocua and one L. seeligeri). Further findings were two (2%) Staphylococcus aureus ST8 with the presence of selx and tsst-1 genes, and five (5%) Bacillus cereus group members (three B. paranthracis, one B. cereus sensu stricto, and one B. cytotoxicus) which all were diarrhea-associated strains.This study provides data that are relevant for HACCP concepts of companies that produce plant-based meat alternative products and helps to define microbiological parameters that should be included when testing such products.

Exploring Protein Dietary Guidance Statements

Consumers may benefit from quick signals on or nearby food product to nudge them toward choosing healthier diets, such as Dietary Guidance Statements (DGSs) from the US Food and Drug Administration. It is unclear how DGSs on foods, including animal or plant proteins, impact purchase motivation, believability, or willingness to pay more. Six DGS claims were tested across 5 protein types (pork tenderloin, beef top sirloin fillet, chicken breast, turkey breast, and plant-based meat) using an online prediction market test survey format among 1002 nationally representative US-based household primary grocery shoppers aged 18 to 64 years. The most motivating and believable DGS across protein types was the Dietary Guidelines DGS: The Dietary Guidelines for Americans recommends eating 5½ oz of protein food per day as part of a nutritious dietary pattern. &lt;protein type&gt; is a lean meat that provides 3 oz of protein food per serving (based on a 2000-calorie diet). More than one-third felt that this Dietary Guidelines DGS was the most motivating and believable because it was informative; imparted feelings of the protein type being healthy; contained statistics, facts, or numbers; and mentioned the US Dietary Guidelines for Americans. More than 50% of participants were willing to pay at least 5% or more for the protein type specified in the Dietary Guidelines DGS across all protein types. Uniform adoption of DGSs across protein food groups allows for equal representation of how a variety of proteins can contribute to a nutritious dietary pattern.

Effects of wheat gluten-soy protein ratios and moisture levels on high-moisture extruded meat analogues for burger patties.

In response to the growing demand for plant-based meat alternatives, this study explores the impact of incorporating wheat gluten (WG) into soy protein isolate (SPI) on both the proximate and functional properties of protein blends. The research also examines the effects of high moisture extrusion processing, varying feed moisture levels (60%, 65%, and 70%), and WG-SPI blends on extruder response, as well as the textural, rheological, and solubility characteristics of the resulting extruded meat analogues. Moreover, the prime objective was to gain insights into the impact of using HMMA made at different ratios and feed moisture levels on plant-based burgers. As the WG incorporation level increased in SPI, the RVA viscosity, water absorption, and oil absorption capacities, and foaming stability exhibited a decrease while foaming capacity increased. As feed moisture and WG incorporation levels in SPI increased, the system parameters, rheological, and textural parameters of high moisture extruded meat analogs decreased. WG25-SPI75 showed the highest degree of texturization or sulfide bonds, and its extruded meat analogues at 60% feed moisture level burger patties resemble a similar textural to a chicken burger patty. This study is pivotal in understanding how wheat gluten in SPIs and feeding moisture level influence the textural and rheology properties of HMMA.

Create the future of food with children: Qualitative insights into children's perception of plant-based meat, fish, and dairy alternatives

Children's food preferences are a major influence on what is served for dinner in the household. However, little is known about children's perceptions of plant-based foods. This study aimed to better understand how 9- to 11-year-old children perceive plant-based meat and dairy alternatives. Different types of plant-based alternatives were investigated: meat and fish substitutes (legumes, nuts), replacements (tofu, tempeh), and analogues, as well as dairy substitutes. These alternatives were placed in a meal context, considering the influence of dish composition. The study combined design probes, interviews, and cooking sessions as different qualitative methods to explore children's perceptions. First, design probe packages with creative assignments were given to the children. Afterwards, one-to-one interviews were conducted to elaborate on the input gained from the assignments. Lastly, children created in small groups a pizza with plant-based alternatives. Results showed that taste was the topic mentioned most in both positive and negative ways. Initially, children generally had positive taste expectations for the analogues, while their taste expectations for substitutes and replacements were more negative. However, during the cooking session, there was no considerable difference in their actual taste perceptions of analogues, substitutes, and replacements. This highlights the importance of studying perceptions of plant-based alternatives in a real-life meal context, where substitutes and replacements can, next to analogues, also be an integral part of a meal. Although previous research shows that animal welfare is an important driver for children to choose plant-based alternatives, this study showed that animal welfare is linked to plant-based meat analogues only. Therefore, actively linking animal welfare to substitutes and replacements may help to guide children and their households towards a plant-forward diet with more plant-based ingredients such as vegetables, legumes, and grains.