Plankton Tows Research Articles

Single chamber Mg/Ca analyses of Globigerinoides ruber for paleo-proxy calibration using femtosecond LA-ICP-MS

Mg/Ca is an independent proxy in paleoceanography to reconstruct past seawater temperature. Femtosecond Laser Ablation Inductively Coupled Plasma Mass Spectrometry (fs-LA-ICP-MS) was employed to determine the Mg/Ca composition of tests (shells) of the planktic foraminifer species Globigerinoides ruber albus (white chromotype) and G. ruber ruber (red/pink chromotype) sampled alive from the temperate to subtropical eastern North Atlantic with the research sailing yacht Eugen Seibold. Mg/Ca data are compared to (i) the measured in-situ temperature of ambient seawater, (ii) average mixed layer temperature, and (iii) sea surface temperature (SST). The pooled mean chamber Mg/Ca from each plankton tow site exhibits a positive relationship with SST. Two chamber-specific calibrations are derived, which are consistent with previous calibration equations for comparable paleo-archives. The results confirm fs-LA-ICP-MS as reliable method for determining Mg/Ca in G. ruber, and both the penultimate and antepenultimate chambers of adult specimens may provide comprehensible Mg/Ca temperatures of the surface ocean.

High‐volume plankton tow net sampling improves eDNA detection of invasive zebra mussels (Dreissena polymorpha) in recently infested lakes

AbstractAquatic invasive species are a serious and growing threat to biodiversity. Zebra and quagga mussels (Dreissena polymorpha and D. rostriformis bugensis) are freshwater invaders causing substantial ecological and economic damage across Europe and North America. Early detection of invasive mussels and other non‐indigenous species is increasingly needed to prevent their establishment and spread. Environmental DNA (eDNA) techniques potentially offer higher sensitivity monitoring tools to complement more conventional methods for surveying adult and juvenile mussels and veliger larvae. eDNA assays are typically performed on small‐volume (0.5–5 L) water samples that are concentrated by filtration prior to extraction and downstream processing. Sampling using a towed plankton net of a larger (64 μm) pore size can process orders of magnitude larger water volumes with the potential for increasing eDNA detection sensitivity. We compared the sensitivities of high‐volume plankton tow net water sampling to filter sampling in three recently infested lakes in Canada and Minnesota, USA. Paired filtration and tow net samples were analyzed for Dreissena DNA using an established quantitative polymerase chain reaction assay for the genus. Higher yields of Dreissena eDNA (more DNA copies) were recovered from plankton tow than from filtered samples in all 33 paired comparisons. In some cases, plankton tow samples were positive for Dreissena eDNA while lower‐volume filtering produced a false negative detection. Our results demonstrate the effectiveness of plankton tow net sampling for eDNA early detection of invasive mussels, a method that can be used exclusively or as a supplement to filter sampling. Our results further suggest that eDNA testing could be incorporated into monitoring programs that routinely use plankton tows for visual detection of invasive mollusk larvae, as well as other aquatic invasive and non‐invasive species ranging from plankton to metazoans, including many fish.

Radiolarian assemblages related to the ocean–ice interaction around the East Antarctic coast

Abstract. The Southern Ocean plays a central role in Earth's climate, ecology, and biogeochemical cycles. Therefore, understanding long-term changes in Southern Ocean water masses in the geologic past is essential for assessing the role of the Southern Ocean in the climate system. Radiolarian fossils are a useful tool to reconstruct the water masses of the Southern Ocean. However, the radiolarian assemblages in the high latitudes of the Southern Ocean (south of the polar front (PF)) are still poorly understood. In this paper, we report the radiolarian assemblages in surface marine sediment and plankton tow samples collected from the high latitudes south of the PF. In the surface sediments, four factors (named F1–F4) of the radiolarian assemblages were identified using Q-mode factor analysis, which are related to different water masses and hydrological conditions. F1 is related to the surface waters south of the southern boundary (SB) of the Antarctic Circumpolar Current (ACC), which are cooled by melting sea ice and ice sheets. F2 is associated with water masses north of the SB. A comparison with the vertical distribution of the radiolarian assemblages in plankton tow samples indicates that characteristic species are associated with the Circumpolar Deep Water (CDW) and surface waters north of the SB. F3 is associated with modified Circumpolar Deep Water (mCDW). The radiolarian assemblage of F4 does not seem specifically related to any of the water mass here analyzed. However, the species in this assemblage are typically dwells within ice shelf and/or sea ice edge environments. Radiolarian assemblages here identified and associated with water masses, and ice edge environments are useful to reconstruct the environment south of the PF in the geologic past.

Analysis of an optical imaging system prototype for autonomously monitoring zooplankton in an aquaculture facility

Traditional approaches to biomonitoring in aquatic systems, such as sample collection, sorting, and identification, require significant time and effort, thereby limiting the spatiotemporal resolution of sample collection. Additionally, collection and preservation of samples for subsequent taxonomic identification and enumeration leads to mortality of organisms. Recent advances in technologies that utilize optical imaging and machine learning have provided new opportunities to expedite biomonitoring and lead to significant cost savings. These technologies can be advantageous to scientists or managers that conduct routine biomonitoring to inform operations, as in the case of aquaculture facilities. The Small Aquatic Organism optical imaging system (SAO) is a high-throughput optical imaging and classification prototype system that relies on computer vision and machine learning (Support Vector Machines, or SVMs) to autonomously identify and enumerate aquatic organisms. The SAO provides a more sustainable method of collecting large volumes of data and has the benefit of being used in situ. In this study, we tested the performance of the SAO in providing comparable results to manual zooplankton community monitoring in ten ponds at an aquaculture facility. We performed a side-by-side study comparing the sampling methods of plankton tow nets, where major zooplankton taxonomic classes were manually identified and enumerated, to sampling with the SAO. Vouchered samples were used to develop a training library for the SAO, where classes consisted of water boatman and zooplankton groups: cladocerans, copepod adults, copepod nauplii, and rotifers. SAO imagery was manually classified and compared with predicted results for validation. Accuracy for the SVM classifier of the SAO was 37.4 %. Convolutional Neural Networks (CNN) and Random Forest classifiers were also applied to SAO imagery and image features for comparison. The best CNN model and our Random Forest model had accuracies of 80.4 % and 46.6 % respectively. Challenges faced included the small size of copepod nauplii and rotifers and the limited resolution of the imaging camera, although there are tradeoffs between imaging resolution and the sample processing rate. Our comparison shows that advancement in both optical imaging and ML are needed in order for the SAO prototype to yield comparable results to manual community monitoring in an aquaculture facility.

Collection of X-ray micro computed tomography images of shells of planktic foraminifera with curated taxonomy

Calcite shells of planktic foraminifera (Protista, Rhizaria) constitute a large portion of deep-sea sediments. The shells are constructed by sequential addition of partly overlapping chambers with diverse shapes, resulting in complex shell architectures, which are genetically fixed and diagnostic at the species level. The characterisation of the complete architecture requires three-dimensional imaging of the shell, including the partially or entirely covered juvenile chambers. Here we provide reconstructed x-ray micro computed tomography image stacks of 179 specimens of extant planktic foraminifera collected from plankton tows, sediment traps and surface sediments. The specimens have fully resolved and curated taxonomy and represent 43 of the currently recognised 48 holoplanktic species and subspecies. The image stacks form a basis for further applications, such as the characterisation of the architectural morphospace of the extant taxa, allowing studies of species functional ecology, calcification intensity and reconstructions of phylogenetic relationships.

Revisiting Oxygen‐18 and Clumped Isotopes in Planktic and Benthic Foraminifera

AbstractForaminiferal isotopes are widely used to study past oceans, with different species recording conditions at different depths. Their δ18O values record both seawater oxygen‐18 and temperature according to species‐specific fractionation factors, while their Δ47 signatures likely depend only on temperature. We describe an open‐source framework to collect/combine data relevant to foraminiferal isotopes, by constraining species‐specific oxygen‐18 fractionation factors (18α) based on culture experiments, stratified plankton tows or core‐top sediments; compiling stratified plankton tow constraints on living depths for planktic species; extracting seawater temperature, δ18O, and chemistry from existing databases for any latitude, longitude, and depth‐range; inferring calcification temperatures based on the above data. We find that although 18α differs between species, its temperature sensitivity remains indistinguishable from inorganic calcite. Based on > 2,600 observations we show that, although most planktic δ18O values are consistent with seawater temperature and δ18O over their expected living depths, a sizable minority (12%–24%) have heavier‐than‐predicted δ18O, best explained by calcification in deeper waters. We use this framework to revisit three recent Δ47 calibration studies of planktic/benthic foraminifera, confirming that planktic Δ47 varies systematically with oxygen‐18‐derived temperature estimates, even for samples whose δ18O disagrees with assumed climatological conditions, and demonstrating excellent agreement between planktic foraminifera and modern, largely inorganic Δ47 calibrations. Benthic foraminifera remain ambiguous: modern benthic Δ47 values appear offset from planktic ones, yet applying equilibrium Δ47 calibration to the Cenozoic benthic foraminifer record of Meckler et al. (2022, https://doi.org/10.1126/science.abk0604) largely reconciles it with δ18O‐derived temperatures, with discrete Δ47/δ18O discrepancies persisting in the Late Paleocene/Eocene/Plio‐Pleistocene.

Radiolaria and Phaeodaria (siliceous Rhizaria) in south-western and northern Norwegian fjords during late summer 2016: dominant species and biomass in shallow-water assemblages

To determine the present-day community composition of siliceous Rhizaria (Radiolaria and Phaeodaria) in Norwegian fjords, plankton tows were conducted in south-western and northern Norwegian fjords in September 2016. The mean total abundance of radiolarians was 306 m–3 in the Sognefjord complex, which was the southern research site, and, in the north, 945 m–3 in Malangen and 89 m–3 in Balsfjord, both above the Arctic Circle. Sticholonche zanclea was the most abundant radiolarian in the Sognefjord complex and Malangen, accounting for 78–100% (mean 89%) of radiolarian abundance. The mean total abundance of phaeodarians was 1554 m–3 in the Sognefjord complex, 51 m–3 in Malangen and 11 m–3 in Balsfjord. Medusetta arcifera was the most abundant phaeodaria in the Sognefjord complex, accounting for >99% of phaeodarian abundance, but was absent in Malangen and Balsfjord, where Protocystis tridens accounted for >96% of phaeodarian abundance. The carbon biomass of S. zanclea and M. arcifera was 188 and 438 µg C m–3, respectively, which is similar to and 8.6 times higher than, respectively, that of phaeodarians >1 mm in the western North Pacific, suggesting that M. arcifera contributes to organic carbon transport in the Sognefjord complex. Amphimelissa setosa (Nassellaria, Radiolaria), which was a dominant species in the study area in 1982–83, was absent in the present study in all sampled fjords. This could have been caused by the approximately 2 °C increase in water temperature that has occurred since 1990 and can be taken as evidence of a climate-change-associated local temperature rise linked to the warming of advected Atlantic Water.

The FORCIS database: A global census of planktonic Foraminifera from ocean waters

Planktonic Foraminifera are unique paleo-environmental indicators through their excellent fossil record in ocean sediments. Their distribution and diversity are affected by different environmental factors including anthropogenically forced ocean and climate change. Until now, historical changes in their distribution have not been fully assessed at the global scale. Here we present the FORCIS (Foraminifera Response to Climatic Stress) database on foraminiferal species diversity and distribution in the global ocean from 1910 until 2018 including published and unpublished data. The FORCIS database includes data collected using plankton tows, continuous plankton recorder, sediment traps and plankton pump, and contains ~22,000, ~157,000, ~9,000, ~400 subsamples, respectively (one single plankton aliquot collected within a depth range, time interval, size fraction range, at a single location) from each category. Our database provides a perspective of the distribution patterns of planktonic Foraminifera in the global ocean on large spatial (regional to basin scale, and at the vertical scale), and temporal (seasonal to interdecadal) scales over the past century.

Planktic foraminifera iodine/calcium ratios from plankton tows

Planktic foraminifera test iodine to calcium ratios represent an emerging proxy method to assess subsurface seawater oxygenation states. Several core-top studies show lower planktic foraminifera I/Ca in locations with oxygen depleted subsurface waters compared to well oxygenated environments. The reasoning behind this trend is that only the oxidized species of iodine, iodate, is incorporated in foraminiferal calcite. The I/Ca of foraminiferal calcite is thought to reflect iodate contents in seawater. To test this hypothesis, we compare planktic foraminifera I/Ca ratios, obtained from plankton tows, with published and new seawater iodate concentrations from 1) the Eastern North Pacific with extensive oxygen depletion, 2) the Benguela Current System with moderately depleted oxygen concentrations, and 3) the well oxygenated North and South Atlantic. We find the lowest I/Ca ratios (0.07 µmol/mol) in planktic foraminifera retrieved from the Eastern North Pacific, and higher values for samples (up to 0.72 µmol/mol) obtained from the Benguela Current System and North and South Atlantic. The I/Ca ratios of plankton tow foraminifera from environments with well oxygenated subsurface waters, however, are an order of magnitude lower compared to core-tops from similarly well-oxygenated regions. This would suggest that planktic foraminifera gain iodine post-mortem, either when sinking through the water column, or during burial.

Large freshwater-influx-induced salinity gradient and diagenetic changes in the northern Indian Ocean dominate the stable oxygen isotopic variation in Globigerinoides ruber

Abstract. The application of stable oxygen isotopic ratio of surface-dwelling planktic foraminifera Globigerinoides ruber (white variety; δ18Oruber) to reconstruct past hydrological changes requires a precise understanding of the effect of ambient parameters on δ18Oruber. The northern Indian Ocean, with its huge freshwater influx and being a part of the Indo-Pacific Warm Pool, provides a unique setting to understand the effect of both the freshwater-influx-induced salinity and temperature on δ18Oruber. Here, we use a total of 400 surface samples (252 from this work and 148 from previous studies), covering the entire salinity end-member region, to assess the effect of freshwater-influx-induced seawater salinity and temperature on δ18Oruber in the northern Indian Ocean. The analysed surface δ18Oruber mimics the expected δ18O calcite estimated from the modern seawater parameters (temperature, salinity, and seawater δ18O) very well. We report a large diagenetic overprinting of δ18Oruber in the surface sediments, with an increase of 0.18 ‰ per kilometre increase in water depth. The freshwater-influx-induced salinity exerts the major control on δ18Oruber (R2=0.63) in the northern Indian Ocean, with an increase of 0.29 ‰ per unit increase in salinity. The relationship between temperature- and salinity-corrected δ18Oruber (δ18Oruber−δ18Osw) in the northern Indian Ocean [T=-0.59⋅(δ18Oruber-δ18Osw)+26.40] is different than reported previously, based on the global compilation of plankton tow δ18Oruber data. The revised equations will help create a better palaeoclimatic reconstruction from the northern Indian Ocean by using the stable oxygen isotopic ratio. The entire data set (newly generated and previously published) used in this work is available both as a Supplement to this article and at PANGAEA (https://doi.org/10.1594/PANGAEA.945401; Saraswat et al., 2022).

Predator-Induced Nocturnal Benthic Emergence: Field and Experimental Evidence for an Unknown Behavioral Escape Mechanism along the Coral Reef–Seagrass Interface

Previously, using plankton tows, and emergence and settlement traps, we documented persistent widespread nocturnal emergence, and planktonic redistribution, of benthic macroinvertebrates along the coral reef–seagrass interface at two geographically separated locations. We also documented that emergence intensity varies with distance from the reef, leading us to hypothesize that the spatial pattern of emergence is determined by the foraging patterns of nocturnally active, bottom-feeding, mid-level consumers (mainly grunts). In this second study, we coupled those previously published data with nocturnal fish surveys concurrently conducted along belt transects placed at the same locations as the emergence trap collections, and a controlled laboratory experiment, to test this hypothesis. The results of these analyses find that variability in the density of nocturnally active, bottom-feeding fish is strongly positively correlated with emergence intensity, regardless of site or season. Results from the laboratory experiments show that nocturnal invertebrate emergence is significantly higher in the presence of one bottom-feeding fish (the blue-striped grunt Haemulon sciurus) than in microcosms that do not contain this predator. Overall, this study shows that such processes may explain how benthic prey can avoid capture by nocturnally active, bottom-feeding predators and persist in the predator-rich seagrass habitats that surround coral reefs in the Florida Keys National Marine Sanctuary. This study also points out the need to consider nocturnal processes when studying seagrass biodiversity in a predator-rich environment.

International shipping as a potent vector for spreading marine parasites.

The global shipping fleet, the primary means of transporting goods among countries, also serves as a major dispersal mechanism for marine invasive species. To date, researchers have primarily focussed on the role of ships in transferring marine macrofauna, often overlooking transfers of associated parasites, which can have larger impacts on naïve host individuals and populations. Here, we re-examine three previously published metabarcode datasets targeting zooplankton and protists in ships' ballast water to assess the diversity of parasites across life stages arriving to three major US ports. Port of Hampton Roads in the Chesapeake Bay, Virginia; Ports of Texas City, Houston and Bayport in Galveston Bay, Texas; and Port of Valdez in Prince William Sound, Alaska. We selected all known parasitic taxa, using sequences generated from the small subunit gene (SSU) from ribosomal RNA (rRNA) amplified from (1) zooplankton collected from plankton tows (35 and 80 μm datasets) and (2) eukaryotes collected from samples of ships' ballast water (3 μm dataset). In all three datasets, we found a broad range of parasitic taxa, including many protistan and metazoan parasites, that infect a wide range of hosts, from teleost fish to dinoflagellates. Parasite richness was highest in the 3 μm dataset and relatively uniform across arrival regions. Several parasite taxa were found in high relative abundance (based on number of sequences recovered) either in ships entering a single or across multiple regions. The ubiquity, diversity and relative abundance of parasites detected demonstrate ships are a potent vector for spreading marine parasites across the world's oceans, potentially contributing to reported increases in outbreaks of marine diseases. Future research is urgently needed to evaluate the fate of parasites upon arrival and the efficacy of ballast water treatment systems to reduce future transfers and colonization.

Spatial variation in food web structure in a recovering marine ecosystem.

Spatial heterogeneity in food web structure and interactions may reconcile spatial variation in population and community dynamics in large marine ecosystems. In order to assess food web contributions to the different community recovery dynamics along the Newfoundland and Labrador shelf ecosystem, we quantified species interactions using stable isotope mixing models and food web metrics within three sub-regions. Representative samples of each species caught in trawls and plankton tows were analyzed for stomach contents and stable isotope ratios (δ15N and δ13C) to parameterize isotope mixing models. Regional variation, highlighted by the diets of three economically important species, was observed such that the southern region demonstrated a variety of trophic pathways of nutrient flow into the higher food web while the diets of fish in the northern regions were typically dominated by one or two pathways via dominant prey species, specifically shrimp (Pandalus sp.) and hyperiids. Food web metrics indicated that the low-diversity northern regions had higher connectance and shorter food chain lengths. This observed regional variation contributes to our understanding of the role of specific forage species to the ecosystem which is an essential contribution towards ecosystem-based management decisions.

Palaeoceanographic significance of aragonitic pteropods in the Indian Ocean – a review

The Quaternary pteropod records published in the late 1990s from the Indian Ocean provided vital information on past oceanographic and climatic changes in this region. This paper reviews the published work on pteropod preservation and abundance in the Indian Ocean to evaluate the usefulness of this microfossil group in palaeoceanographic reconstructions. Their aragonitic shell chemistry makes them reliable indicators of ocean acidification, paleaoceanography and paleoclimate. An increased interest in studying this faunal group has arisen from recent findings on the vulnerability of aragonite shell preservation to human impacted water column chemistry changes. The compiled pteropod studies show that most of the pteropod databases contain information on abundance and distribution. However, the biogeochemical parameters and processes, such as aragonite production in the water column, their settling fluxes and the shell dissolution patterns are less explored. Despite the wide geographical range of pteropods and their significance in the marine food web, the ecology and taxonomy of the holoplanktonic gastropods in the Indian Ocean are poorly known. Though these pteropods are recovered in plankton tows (standing crop) and sediment traps (settling fluxes), the surface sediments in large parts of the northern Indian Ocean are completely devoid of them. Thus, assessing their fate in the water column and the sediment–water interface and their response to prevailing biogeochemical conditions are worth pursuing. Genomic studies and isotopic and elemental chemistry of living and fossil pteropods could help assess their response to ecological changes. To date, such modern techniques have been little utilized in studying Indian Ocean pteropods. A knowledge of their shell chemistry is crucial in the context of increased input of anthropogenic CO2 and the susceptibility of pteropod preservation to changing climate.

Morphotype and Crust Effects on the Geochemistry of Globorotalia inflata

AbstractSedimentary specimens of the planktonic foraminifera Globorotalia inflata can provide information on subsurface conditions of past oceans. However, interpretation of their geochemical signal is complicated by possible effects of cryptic diversity and encrustation. Here we address these issues using plankton tow and sediment samples from the western South Atlantic, where two genotypes of G. inflata occur. To separate the genotypes, we analyzed shells from the extreme ends of the morphospace of G. inflata. The δ18O and δ13C of encrusted specimens from both morphotypes are indistinguishable. However, we do find a large influence of encrustation on δ18O and Mg/Ca. Whereas crust Mg/Ca ratios are at all locations lower than lamellar calcite, the crust effect on δ18O is less consistent in space. Plankton tows show that encrusted specimens occur at any depth and that even close to the surface crust Mg/Ca ratios are lower than in lamellar calcite. This is inconsistent with formation of the crust at lower temperature at greater depth. Instead we suggest that the difference between the crust and lamellar calcite Mg/Ca ratio is temperature‐independent and due to the presence of high Mg/Ca bands only in the lamellar calcite. The variable crust effect on δ18O is more difficult to explain, but the higher incidence of crust‐free specimens in warmer waters and the observation that a crust effect is clearest in the confluence zone, hint at the possibility that the difference reflects advective mixing of specimens from warmer and colder areas, rather than vertical migration.

In Situ Mg/Ca Measurements on Foraminifera: Comparison Between Laser Ablation Inductively Coupled Plasma Mass Spectrometry and Wavelength‐Dispersive X‐Ray Spectroscopy by Electron Probe Microanalyzer

AbstractWe present a comparison of two different techniques: Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA‐ICP‐MS) and wavelength‐dispersive X‐Ray Spectroscopy by electron probe microanalyzer (EPMA) for obtaining Mg/Ca ratios in individual foraminifera shells. The goal is to assess the use of EPMA as an alternative technique for Mg/Ca analyses of single foraminiferal calcite shells. Foraminifera obtained from sediments (benthic, Uvigerina spp.) and from plankton tows (planktonic, Orbulina universa) were analyzed. All specimens were prepared in epoxy mounts and exposed in cross‐section such that multiple high‐resolution analyses could be completed on the shells using both techniques. We examined our data using statistical methods designed for the assessment and comparison of measurement techniques. In the case of Uvigerina, the mean difference for ratios obtained using EPMA and LA‐ICP‐MS is very small (−0.046 mmol mol−1) and scale independent. The Limits of Agreement (LoA, the standard deviation of the bias plus the mean bias) is [−0.315, 0.223] mmol mol−1. For samples with ratios lower than 13 mmol mol−1, we found a mean EPMA–LA‐ICP‐MS bias of −2.44 mmol mol−1 and a corresponding LoA of [−3.85, −1.04] mmol mol−1. For ratios higher than 13 mmol mol−1, there appears to be a scale dependent bias, meaning that the EPMA measured ratios become progressively larger than those of LA‐ICP‐MS as the Mg/Ca ratio increases, so the mean bias and LoA metrics are not meaningful. Results indicate that it is possible to use EPMA to collect Mg/Ca data, if the ratios are lower than ∼13 mmol mol−1.

The Horizontal Distribution of Siliceous Planktonic Radiolarian Community in the Eastern Indian Ocean

The plankton radiolarian community was investigated in the spring season during the two-month cruise ‘Shiyan1’ (10 April–13 May 2014) in the Eastern Indian Ocean. This is the first comprehensive plankton tow study to be carried out from 44 sampling stations across the entire area (80.00°–96.10° E, 10.08° N–6.00° S) of the Eastern Indian Ocean. The plankton tow samples were collected from a vertical haul from a depth 200 m to the surface. During the cruise, conductivity–temperature–depth (CTD) measurements were taken of temperature, salinity and chlorophyll a from the surface to 200 m depth. Shannon–Wiener’s diversity index (H’) and the dominance index (Y) were used to analyze community structure. There was a total of 168 plankton species, composed of Acantharia, Phaeodaria, Polycystina, Collodaria and Taxopodida (monospecific—Sticholonche zanclea, Hertwig is the only recognized species). Hence, it included both celestine-based and siliceous organisms, which are also described here for the first time from this region. Total radiolarians ranged from 5 to 5500 ind/m−3, dominated by co-occurrences of Sphaerozoum punctatum and Stichonche zanclea species at the south-equator zone (SEQ)-transect 80° E and equator zone (EQ)-transect Lati-0. The possible environmental variables were tested through RDA analysis; although no result was obtained for the full species dataset, the samples from the equatorial transect related strongly to mixed-layer chlorophyll a concentration and those of a north–south transect to surface silicate concentrations or mixed-layer nitrate were significantly correlated (p < 0.01) to the radiolarian community. Our results indicate that the silicate and chlorophyll-a concentrations are the two major factors affecting the radiolarian distribution along two of the investigated transects (southern equator and equator) in the study area.

High‐Resolution Mg/Ca and δ18O Patterns in Modern Neogloboquadrina pachyderma From the Fram Strait and Irminger Sea

AbstractNeogloboquadrina pachyderma is the dominant species of planktonic foraminifera found in polar waters and is therefore invaluable for paleoceanographic studies of the high latitudes. However, the geochemistry of this species is complicated due to the development of a thick calcite crust in its final growth stage and at greater depths within the water column. We analyzed the in situ Mg/Ca and δ18O in discrete calcite zones using laser ablation‐inductively coupled plasma‐mass spectrometry, electron probe microanalysis, and secondary ion mass spectrometry within modern N. pachyderma shells from the highly dynamic Fram Strait and the seasonally isothermal/isohaline Irminger Sea. Here we compare shell geochemistry to the measured temperature, salinity, and δ18Osw in which the shells calcified to better understand the controls on N. pachyderma geochemical heterogeneity. We present a relationship between Mg/Ca and temperature in N. pachyderma lamellar calcite that is significantly different than published equations for shells that contained both crust and lamellar calcite. We also document highly variable secondary ion mass spectrometry δ18O results (up to a 3.3‰ range in single shells) on plankton tow samples which we hypothesize is due to the granular texture of shell walls. Finally, we document that the δ18O of the crust and lamellar calcite of N. pachyderma from an isothermal/isohaline environment are indistinguishable from each other, indicating that shifts in N. pachyderma δ18O are primarily controlled by changes in environmental temperature and/or salinity rather than differences in the sensitivities of the two calcite types to environmental conditions.

Eukaryotic plankton communities across reef environments in Bocas del Toro Archipelago, Panamá

Variation in light and temperature can influence the genetic diversity and structure of marine plankton communities. While open-ocean plankton communities receive much scientific attention, little is known about how environmental variation affects plankton communities on tropical coral reefs. Here, we characterize eukaryotic plankton communities on coral reefs across the Bocas del Toro Archipelago, Panama. Temperature loggers were deployed, and midday light levels were measured to quantify environmental differences across reefs at four inshore and four offshore sites (Inshore = Punta Donato, Smithsonian Tropical Research Institute (STRI) Point, Cristobal, Punta Laurel and Offshore = Drago Mar, Bastimentos North, Bastimentos South, and Cayo de Agua). Triplicate vertical plankton tows were collected midday, and high-throughput 18S ribosomal DNA metabarcoding was leveraged to investigate the relationship between eukaryotic plankton community structure and inshore/offshore reef environments. Plankton communities from STRI Point were additionally characterized in the morning (~ 08:00), midday (~ 12:00), and late-day (~ 16:00) to quantify temporal variation within a single site. We found that inshore reefs experienced higher average seawater temperatures, while offshore sites offered higher light levels, presumably associated with reduced water turbidity on reefs further from shore. These significant environmental differences between inshore and offshore reefs corresponded with overall plankton community differences. We also found that temporal variation played a structuring role within these plankton communities, and conclude that time of community sampling is an important consideration for future studies. Follow-up studies focusing on more intensive sampling efforts across space and time, coupled with techniques that can detect more subtle genetic differences between and within communities will more fully capture plankton dynamics in this region and beyond.

Microplastic Prevalence in 4 Oregon Rivers Along a Rural to Urban Gradient Applying a Cost‐Effective Validation Technique

Microplastics are ubiquitous in our environment and are found in rivers, streams, oceans, and even tap water. Riverine microplastics are relatively understudied compared with those in marine ecosystems. In Oregon (USA), we sampled 8 sites along 4 freshwater rivers spanning rural to urban areas to quantify microplastics. Plankton tow samples from sites along the Columbia, Willamette, Deschutes, and Rogue Rivers were analyzed using traditional light microscopy for initial microplastic counts. Application of Nile Red dye to validate microplastics improved microplastic identification, particularly for particles (Wilcox test; p = 0.001). Nile Red-corrected microfiber abundance was correlated with human population within 5 km of the sample site (R² = 0.554), although no such relationship was observed between microparticles and population (R² = 0.183). We found that plastics were present in all samples from all sites, despite the range from undeveloped, remote stretches of river in rural areas to metropolitan sites within Portland (OR, USA), demonstrating the pervasive presence of plastic pollution in freshwater ecosystems. Environ Toxicol Chem 2020;39:1590-1598. © 2020 SETAC.