Event Abstract Back to Event Adjuvants as possible solution to polysaccharide unconjugated vaccines Belkis Romeu1*, Miriam Lastre1, Osmir Cabrera1, Elizabeth Gonzalez1, Aleida Mandariote1, Luis García1, Daniel Cardoso1 and Oliver Pérez1 1 Finlay Institute, Immunology Department, Cuba Background. Plain polysaccharide (Ps) vaccines were the first meningococcal vaccines developed. Nevertheless, they neither function in new born / infant nor induce memory response and could induce antibody hyporesponsiveness. Therefore, covalent conjugated vaccines offer a particular type of stimulation where the carrier gives the T cell help. However, adjuvants in the restricted sense never were considered as an alternative solution. Therefore, we developed a proprietary adjuvant Finlay platform derived from Neisseria meningitidis. Aims. To determine if adjuvants could be use as an alternative solution to covalent conjugated Ps vaccines. Results. The Ps used were: PsVi from Salmonella Typhi and PsA from N. meningitidis serogroups A, respectively. The adjuvants used were: Outer membrane vesicles from N. meningitidis serogroups A, W and AFCo (Cochleate) 1 derived from PL. MenAfricVac™ as well as unconjugated plain Ps were use as controls. Anti Ps IgG class and IgG1, IgG2a, and IgG3 subclasses were evaluated after two parenteral or three mucosal doses. The conventional memory response was evaluated after a booster dose. Non-conventional T and B memory response was also evaluated. OMV and AFCo1 overcome the T-independence of Ps inducing a Th1 typical pattern. They induce memory response at B and T cell levels. Conclusion. Adjuvants could be an alternative to covalent conjugated vaccines. The mechanism of T cell memory induction needs to be fully explored. Acknowledgements We thank the Finlay Institute for supporting this work. Keywords: adjuvant, Vaccine, Protoliposome, Cochleate, OMV Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Romeu B, Lastre M, Cabrera O, Gonzalez E, Mandariote A, García L, Cardoso D and Pérez O (2013). Adjuvants as possible solution to polysaccharide unconjugated vaccines. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01074 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Belkis Romeu, Finlay Institute, Immunology Department, Havana, Havana, Cuba, bromeu@finlay.edu.cu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Belkis Romeu Miriam Lastre Osmir Cabrera Elizabeth Gonzalez Aleida Mandariote Luis García Daniel Cardoso Oliver Pérez Google Belkis Romeu Miriam Lastre Osmir Cabrera Elizabeth Gonzalez Aleida Mandariote Luis García Daniel Cardoso Oliver Pérez Google Scholar Belkis Romeu Miriam Lastre Osmir Cabrera Elizabeth Gonzalez Aleida Mandariote Luis García Daniel Cardoso Oliver Pérez PubMed Belkis Romeu Miriam Lastre Osmir Cabrera Elizabeth Gonzalez Aleida Mandariote Luis García Daniel Cardoso Oliver Pérez Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.