The immersion of the face in cold water evokes a powerful autonomic reflex known as the diving response. It is activated primarily by stimulation of the trigeminal nerve (TGS) that innervates the areas around the forehead and cheeks, but its physiological effects are also modulated by secondary mechanisms such as apnoea. The diving response is believed to act to preserve oxygen delivery to the heart and brain. We sought to determine the relative importance of the TGS and apnoea components of the diving response to the regulation of cerebral blood flow.In 8 young, healthy volunteers (aged 24 ± 3 yr; 2 women), middle cerebral artery velocity (MCAV; transcranial Doppler), arterial blood pressure (Finometer), heart rate (ECG) and the partial pressure of end‐tidal carbon dioxide (PETCO2) were recorded. In addition, internal carotid artery diameter and velocity were measured (ICAV) using duplex Doppler ultrasound, and internal carotid artery flow (ICAQ) calculated. Cerebral vascular conductance (CVC) was calculated as; MCAV or ICAQ/MAP. TGS was evoked by the placement of ice packs on the forehead and cheeks under two conditions; 1) while performing an end‐expiratory breath‐hold (+BH), and 2) while breathing spontaneously (‐BH). The second condition was always undertaken last and its duration was matched to that of the first condition. Data were averaged at baseline (3 min) and at the end of each manoeuvre (last 10 cardiac cycles) and are presented as mean ± SEM.The apnoea was held for 26 ± 3 s. During combined TGS+BH, mean blood pressure (88 ± 5 vs. 101 ± 4 mmHg), MCAV (67 ± 9 vs. 82 ± 8 cm s−1) and ICAQ (309 ± 42 vs. 386 ± 56 ml min−1) were increased (p<0.05), while MCACVC (0.77 ± 0.11 vs 0.81± 0.08 cm s−1/mmHg) and ICACVC (3.49 ± 0.47 vs. 3.84 ± 0.60 ml min−1/mmHg) remained unchanged (P<0.05), and heart rate tended to decline (71 ± 2 vs. 65 ± 3 bpm; P=0.072). In contrast, during TGS–BH, mean blood pressure increased (87 ± 2 vs. 91 ± 2 mmHg: P<0.01), while MCAV (65 ± 7 vs. 66 ± 7 cm s−1), MCACVC (0.75 ± 0.08 vs. 0.73 ± 0.08 cm s−1/mmHg), ICAQ (304 ± 40 vs. 296 ± 41 ml min−1), ICACVC (3.38 ± 0.48 vs. 3.17 ± 0.47 ml min−1/mmHg) and heart rate (67 ± 4 vs. 66 ± 4 bpm) remained unchanged (P>0.05).In summary, cerebral blood flow increased when TGS was coupled with an apnoea, but the independent activation of TGS did not elicit an increase in cerebral blood flow. These findings suggest that physiological factors associated with breath holding (e.g., pressor response, CO2 accumulation) make the predominate contribution to diving response mediated‐increases in cerebral blood flow in humans.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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