Due to its unique mechanical, thermal, photocatalytic, and antibacterial qualities, the ground-breaking bio-based polymer nanocomposite polylactic acid/titanium dioxide (PLA/ TiO2) is a well-known replacement for conventional plastics and is being actively researched. In this work, a route is developed to synthesize nanocomposites based on TiO2-strengthened PLA nanocomposites (PLA/ TiO2). The synthesized nanocomposite was characterized using various spectroscopic techniques such as Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Thermogravimetric Analysis (TGA) and Transmission electron microscopy (TEM). Using a gas permeameter, the samples oxygen permeability was determined. Water molecules were able to readily penetrate the nanocomposite, and PLA/ TiO2 nanocomposites degraded more quickly than pure PLA. The dispersion of the TiO2 nanoparticles affected the rate of PLA degradation. To regulate the rate at which PLA breaks down during the composting process, a certain amount of dispersed TiO2 nanofiller may also be applied. The photocatalytic activity of the resulting nanocomposites was investigated by monitoring the degradation of methyl orange and malachite green. The PLA/ TiO2 nanoparticles exhibit antibacterial properties as indicated by their zone of inhibition with in addition, PLA/ TiO2 nanoparticles. decrease the amount of extracellular polymeric substance (EPS), indicating up to 17 mm diameter. Their anti -virulence and bio film inhibitory nature, indicating it's potential application as surface disinfectant.