Abstract Purpose: The Detection of Protein Kinase C Isoenzymes in Patients at risk of Schistosoma haematobium induced Bladder Cancer attending University of Maiduguri Teaching Hospital (UMTH) was carried out. The study comprised of fifty six (56) patients aged ten to fifty six (10-56) years with clinical features of schistosomiasis and confirmed for the presence of S .haematobium eggs in urine samples. Methods: Twenty milliliters (20ml) of urine was centrifuged at 1000 rpm for 10 min to obtain the sediment. The supernatants were then discarded and the sediment (pellet) re-suspended for S. haematobium. A drop of the sediment were transferred to a glass slide, covered with a cover slip and examined using × 40 objective lens. The urine samples were observed for red blood cells and deposits that might contain proteins. The extraction of total urine template RNA was carried out according to Bioneer Specification and protocol; fifty six (56) of the S. haematobium samples of the patients were used. AccuPrep® Universal RNA Extraction Kit (K-3141) from Bioneer Corporation was used and one page protocol was followed for RNA Extraction. Pre- Denaturation was done for 5min at 94oC, Denaturation for 30sec at 94oC, Annealing for 30sec at 52oC, Extension for 1min at 72oC 35 cycles, Final extension for 5min at 72oC. Results: Result was run on 2% agarose gel. The study was able to investigate and detect the putative involvement of different PKC isoforms (alpha, beta, and delta) in patient with risk of schistosoma related bladder cancer. Out of the fifty six (56) Multiplex PCR Amplification of PKC Alpha, PKC Beta and PKC Delta from cDNA of extracted RNA template of S. haematobium infected urine sample investigated, only twenty seven (27) showed detection base pairs predicted size from Lane 1-9; of which PKC Alpha (325bp) has two (2) lane; PKC Beta (490bp) has seven (7) lane; and PKC Delta (404bp) has eighteen (18) lane of predicted base pair according to the cDNA size marker, some empty-zero (0) bands lanes and Unpredicted base pair band size were also seen. Conclusion: The major findings in the present study were the detection of PKC isoenzymes base pairs (band) which indicated the risk level of schistoma induced bladder cancer and stages of the patients using the analysis that proves Isoenzymes are detected by electrophoresis. Snail control, sanitation, avoid contacts with bullinus species and focus on early detection and diagnosis of patients infected with S. haematobium. Citation Format: Zanna Abdulsalam, Adamu Inuwa, Sambo Bello Zailani. Detection of Protein Kinase C Isoenzymes in Patients at Risk of Schistosoma Haematobium Induced Bladder Cancer [abstract]. In: Proceedings of the 11th Annual Symposium on Global Cancer Research; Closing the Research-to-Implementation Gap; 2023 Apr 4-6. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2023;32(6_Suppl):Abstract nr 29.