The analysis of the current and cancelled documents concerning the regulation of working conditions in civil aviation (CA) is carried out. There have been established significant factors of working environment and workload, their relationship with indicators of the health status of flight personnel, the development of fatigue and the risks of aviation accidents (AA) has been proved. The design of the study included a multifactorial analysis of the impact of working conditions on the functional state, occupational morbidity and accident rate of CA pilots with the establishment of a list of mandatory factors for rationing and control of the production environment and the labour process. The analysis of sanitary and hygienic characteristics of working conditions, health data was carried out based on the results of retrospective studies of the clinic of the Federal State Budgetary Scientific Institution “Izmerov Research Institute of Occupational Health”, publications indexed in the databases Scopus, Web of Science, RSCI and official statistical collections. Own experimental studies were conducted among one hundred twenty pilots of the case-control type, including the analysis of 10324 standard operating procedures for aircraft management, 59670 studies within the framework of psychophysiological testing, as well as the analysis of 83 AA. The necessity of adjusting the regulatory framework with the expansion of indicators for the normalization of working conditions in terms of the intensity of the labour process is proved. New criteria are proposed to be used for assessing information, intellectual, emotional loads, expanding the boundaries of sensory loads with the allocation of class 3.3 - according factors working load. Proposals have been developed to make additions to SP 2.5.3650-20 for flight crew members and air traffic controllers concerning requirements for all missed and obligatory factors of working conditions; a list of production factors obligatory for control during a special assessment of working conditions at the workplaces of aircraft crew members; new methodological documents relating to the assessment of indicators of the intensity of the labour process, the assessment of the personal risk of fatigue in pilots, conducting psychophysiological studies using a video oculograph, the procedure for conducting special assessment of working conditions, a general assessment of professional risks intended for the implementation of regulatory requirements.