Abstract–Triplet‐minus‐singlet (T‐S) spectra and singlet‐triplet annihilation was measured on Rhodospirillum rub‐rum chromatophores at low temperatures (4–77K). The T‐S spectrum of the LH‐1 antenna was dependent on the wavelength of excitation and thus indicative of site‐inhomogeneous broadening. The data cannot be understood adequately using the two pigment pool model (B880 + B896) but, in contrast, support a model for LH‐1, in which the spectral properties are determined by site‐inhomogeneous broadening. Consequently, the 4 K antenna domain for singlet‐triplet annihilation in LH‐1 is rather small. If the temperature is raised to 77 K the annihilation domain increases, and a fit of the fluorescence quenching and triplet yield data suggests that at 77 K the connectivity is between the extreme cases of the “lake” and the isolated “puddles” models. The implications of the observed antenna inhomogeneity on low temperature annihilation and fluorescence measurements are discussed.