l-Histidine/ d-histidine-imprinted membranes have been synthesized by electropolymerizing acrylamide onto Au electrodes or Au-coated quartz crystal electrodes in the presence of l-histidine/ d-histidine. AC impedance spectroscopy and piezoelectric quartz crystal (PQC) technique were employed to verify the template effect of the membranes. The selectively rebinding of the enantiomers of histidine by their respective imprinted polymer membranes was confirmed. The result may lead to the construction of biomimetic sensors for the stereospecific determination of l-histidine and the chiral separation of histidine.