Plants For Phytoremediation Research Articles

Phytostabilisation of arsenic contaminated gold mine waste using the native species Juncus usitatus, Poa labillardieri and Themeda triandra

Arsenic (As) contaminated gold mine waste represents a significant issue for ecosystem and human health, as it is a known carcinogen and environmental toxin. Bendigo in Victoria, Australia, has reported As concentrations up to 22,000 mg/kg, exceeding the national soil guidance values (100 mg/kg) by 220-fold. To reduce exposure and potential human health risk, remediation or risk management strategies are required. Traditional remediation, such as excavation and landfill, often fail to improve soil conditions and are expensive. Biological remediation using plants (phytoremediation) represents a cost-effective strategy that results in the restoration of soil function and ecosystem services. There is a need to identify native plants for phytoremediation as current research focuses on fern species, which are not suitable for Bendigo. This study aimed to identify a native phytostabilising plant by conducting a mesocosm experiment involving Juncus usitatus, Poa labillardieri, and Themeda triandra. Plants grew in mine waste for 100 days; all survived and bioaccumulated As in the roots (phytostabilisation). Poa labillardieri grew significantly more biomass (roots 0.24 ± 0.01 g; shoots 0.22 ± 0.03 g (d/w)) and bioaccumulated the most As (~ 100 mg/kg plant biomass). The bacterial community was investigated to assess soil health and As association. The rhizosphere microbiota stabilised after root colonisation for Poa labillardieri and Themeda triandra, as indicated by richness and evenness changes. The two dominant phyla were Actinobacteriota and Proteobacteria, which displayed As tolerance. A keystone taxon, Latescibacterota, exhibited a positive relative association with As remediation. Poa labillardieri is a good candidate for future phytostabilisation trials.

Phytoremediation of contaminated urban soils spiked with heavy metals

Urban environments worldwide face toxic heavy metal pollution originating from industrial discharge, municipal waste disposal, vehicular emissions, and atmospheric deposition. Kazakhstan, experiencing accelerated economic growth and extensive mining activities, contends with widespread heavy metal contamination in its soil-plant-air-water ecosystems. This study explores the potential of hyperaccumulating plants for phytoremediation in urban soils of Kazakhstan contaminated with Pb, Cd, and Co. Twelve plant species, including Korean Mint (Lamiaceae), Ornamental Cabbage (Brassica oleracea), Ageratum (Ageratum houstonianum), Coneflower (Echinacea purpurea), Amaranth (Amaranthus Perfect and Amaranthus Emerald), Fescue (Festuca glauca), Burning Bush (Kochia scoparia), Marigold (Tagetes patula nana), White Cabbage (Brassica-Cavolo cappuccino BIANKO), Tepary Bean (Phaseolus acutifolius), and Rapeseed (Brassica napus), were evaluated for growth and biomass production in urban soils spiked with two maximum permissible addition (MPA) treatments of Pb, Co, and Cd. The selected plants demonstrated varied responses to heavy metal stress, with Marigold (8.4 g shoot biomass/plant), Korean mint (10.5 g shoot biomass/plant), Rapeseed (19.9 g/shoot biomass), and Tepary bean (25.9 g shoot biomass/plant) exhibiting resilience or tolerance to Pb, Co, and Cd stresses. The results highlight the significant potential of these plants for efficient phytoremediation, showcasing their unique abilities to absorb and accumulate specific metals. Marigold, particularly, displayed noteworthy Pb accumulation (40.3 mg/kg biomass), resulting in reduced residual Pb concentrations in the soil (74.7 mg/kg). Conversely, White cabbage and Amaranth showed limited efficiency in Cd extraction, while Rapeseed and Tepary bean emerged as promising candidates for Cd phytoremediation. This study emphasizes the critical role of tailored plant species selection in designing effective phytoremediation strategies for specific metal-contaminated urban sites. A comprehensive understanding of the dynamics of metal accumulation and residual concentrations is crucial for the development of sustainable and efficient environmental remediation approaches. Further research is warranted to explore the long-term effects of different plant species on soil metal concentrations, refining and optimizing phytoremediation methods for urban soils grappling with toxic heavy metal contamination.

PHYTOREMEDIATION PROSPECTS FOR SOILS CONTAMINATED WITH HEAVY METALS

Phytoremediation is an effective technique of using plants to restore contaminated soils. The aim of this work is to review promising hyperaccumulators with regard to phytoremediation. Different types of plants, genetic and cellular engineering and other techniques that contribute to soil purification from heavy metals are used for phytoremediation. The paper presents an overview of hyperaccumulators that are able to accumulate various contaminants in the tissues of aboveground organs. The literature search was carried out in such databases as eLIBRARY and CyberLeninka. Plants for phytoremediation should meet a large set of specific criteria. However, no plant can satisfy all of them. Therefore, choosing the most effective plant is a difficult task. The paper analyzes plants used for phytoremediation on the territory of the Russian Federation. The list of studies included in the review demonstrates the peculiarities of certain heavy metal accumulation by different plants. At the same time, the majority of plants used for phytoremediant (about 75 %) accumulate nickel. Only a small number of plants are able to accumulate other heavy metals, such as copper, zinc, cadmium, and lead. Some publications presented in the review indicate that for effective phytoremediation, it is also necessary to pay attention to the soil composition and other external factors. This remark is really crucial. Currently, phytoremediation is an understudied unpredictable technique for cleaning up contaminated soils. It has not received widespread use yet, but it is promising.

Zinc Accumulation Pattern in Native Cortaderia nitida in High Andes (Ecuador) and Potential for Zinc Phytoremediation in Soil

The aim of this work was to determine the content of heavy metals in soil and, for the first time, in wild Cortaderia nitida, and to discuss its potential as a metal phytoremediator plant. We sampled sediments (bulk and rhizosphere) and C. nitida (roots and shoots) in three nearby spots with different land uses (urban, industrialized and agricultural) along the Chibunga river basin (Ecuador). We analyzed the physico-chemical parameters in soil and heavy metal contents in soil and plants. The agricultural sediments showed the highest conductivity and redox potential, but the lowest pH. Among all the metals analyzed in soil and plants, we only found significant values of Zn and Fe. We observed clear differences in patterns of Zn distribution throughout soil and plants among the three areas sampled, thus suggesting that soil properties played an important role in Zn compartmentalization. Also, C. nitida demonstrated effective Zn translocation from roots to shoots, especially in farmlands (translocation factors between 1.64 and 2.51). Together with the results obtained for other Cortaderia species in metal-polluted areas, this study proposes C. nitida as a candidate to further study its metal phytoremediation potential and encourages this research in heavy metal-enriched soils.

Invasive Amaranthus spp. for heavy metal phytoremediation: Investigations of cadmium and lead accumulation and soil microbial community in three zinc mining areas

Amaranthus spp. are a group of strongly invasive and vigorous plants, and heavy metal phytoremediation using alien invasive Amaranthus spp. has been a popular research topic. In this study, the bioconcentration factor (BCF) and translocation factor (TF) of Amaranthus spp. were evaluated, focusing on the accumulation potential of cadmium (Cd) and lead (Pb) by plants from three different zinc mining areas, namely Huayuan (HYX), Yueyang (LYX), and Liuyang (LYX). The HYX area has the most severe Cd contamination, while the LYX area has the most apparent Pb contamination. The results showed that Amaranthus spp. had a strong Cd and Pb enrichment capacity in low-polluted areas. To elucidate the underlying mechanisms, we used high-throughput sequencing of 16S rRNA and internal transcribed spacer (ITS) regions to analyze rhizosphere bacterial and fungal communities in three areas. The results showed significant differences in the structure, function, and composition of microbial communities and complex interactions between plants and their microbes. The correlation analysis revealed that some key microorganisms (e.g., Amycolatopsis, Bryobacterium, Sphingomonas, Flavobacterium, Agaricus, Nigrospora, Humicola) could regulate several soil factors such as soil pH, organic matter (OM), available nitrogen (AN), and available phosphorus (AP) to affect the heavy metal enrichment capacity of plants. Notably, some enzymes (e.g., P-type ATPases, Cysteine synthase, Catalase, Acid phosphatase) and genes (e.g., ZIP gene family, and ArsR, MerR, Fur, NikR transcription regulators) have been found to be involved in promoting Cd and Pb accumulation in Amaranthus spp. This study can provide new ideas for managing heavy metal-contaminated soils and new ways for the ecological resource utilization of invasive plants in phytoremediation.

Varying Tolerance to Diesel Toxicity Revealed by Growth Response Evaluation of Petunia grandiflora Shoot Lines Regenerated after Diesel Fuel Treatment

Continuous efforts are required to find ways to protect crop production against the toxicity of petroleum hydrocarbons, such as diesel, and contamination of soils. There is a need for identification of candidate plants that are tolerant to diesel toxicity that might also have the potential for remediation of diesel-contaminated soils. In this study, petunia, a popular ornamental plant and a model experimental plant in research on phytoremediation of environmental pollutants, was used to evaluate a novel method for rapidly assessing diesel toxicity based on the tolerance of shoots generated through in vitro plant cell culture selection. Petunia shoot lines (L1 to L4) regenerated from diesel-treated callus were compared with those from non-diesel-treated callus (control). Significant morphological differences were observed among the tested lines and control, notably with L1 and L4 showing superior growth. In particular, L4 exhibited remarkable adaptability, with increased root development and microbial counts in a diesel-contaminated potting mix, suggesting that the shoots exhibited enhanced tolerance to diesel exposure. Here, this rapid bioassay has been shown to effectively identify plants with varying levels of tolerance to diesel toxicity and could therefore assist accelerated selection of superior plants for phytoremediation. Further research is needed to understand the genetic and physiological mechanisms underlying tolerance traits, with potential applications beyond petunias to other environmentally significant plants.

Long-term adaptation of water hyacinth to low cadmium involves antioxidant enzyme and metallothionein transcriptional regulation

Cadmium (Cd) is one of the main heavy metal pollutants in environment. Water hyacinth (Eichhornia crassipes) is an effective phytoremediation plant for mitigating Cd stress, though the concentration threshold for its long-term survival remains unclear. Our results indicate that 4 mg L−1 Cd may be the maximum threshold for long-term cultivation of water hyacinth, as it significantly inhibits root growth and photosynthesis. The activity of superoxide dismutase increased under low concentration Cd treatment (0.5 and 1.0 mg L−1), potentially contributing to the reactive oxygen species (ROS) homeostasis in water hyacinth. Additionally, we identified the Cd-induced metallothionein gene MT1, whose heterologous expression in yeast enhanced Cd tolerance despite higher Cd accumulation. The upregulation of MT1 may enhance the detoxification capacity of water hyacinth under Cd stress. Our findings establish the Cd range for long-term cultivation of water hyacinth and elucidated the mechanism of its tolerance to moderate Cd stress.

Decrease due to pollution in the rhizosphere microbial diversity can be amended by supplementation from adapted plants of another species

Manipulating the rhizosphere microbiome to enhance plant stress tolerance is an environmentally friendly technology and a renewable resource to restore degraded environments. Here we suggest a sustainable bioremediation strategy on the example of Stebnyk mine tailings storage. We consider Salicornia europaea rhizosphere community, and the ability of the phytoremediation plant Salix viminalis to recruit its beneficial microbiome to mediate the pollution stress at the Stebnyk mine tailings storage. The tailings contain large amounts of brine salts and heavy metals that contaminate the ground water and surrounding areas, changing soil biogeochemistry and causing increased erosion. The species richness of the endophytic bacterial community of S. viminalis roots was assessed based on observed OTUs, Shannon-InvSimpson, and evenness index. Our results obtained using the plant-based enrichment strategy show that biodiversity was decreased across the contamination zones and that S. europaea supplementation significantly increased the species richness. Our results also indicate that the number of dominating bacteria was not changed across zones in both S. europaea-treated and untreated bacterial populations, and that the decrease in richness was mainly caused by the low abundant bacterial OTUs. The importance of selecting the bioremediation strains that are likely to harbor a reservoir of genetic traits that aid in bioremediation function from the target environment is discussed

Enhancing the phytoremediation efficiency of Bacopa monnieri (L.) Wettst. using LED lights: a sustainable approach for heavy metal pollution control.

In this study, the impacts of LEDs on the phytoremediation of arsenic (As) and mercury (Hg) by Bacopa monnieri (L.) Wettst. were investigated, along with the examination of the biochemical characteristics of plants exposed to metal-induced toxicity. In vitro multiple and rapid plant propagations were successfully achieved by adding 1.0mg/L 6-Benzyl amino purine (BAP) to the Murashige and Skoog (MS) basal salt and vitamin culture medium. For plant-based remediation experiments, different concentrations of As (0-1.0mg/L) and Hg (0-0.2mg/L) were added to the water environment, and trials were conducted for four different application periods (1-21days). White, red, and blue LEDs, as well as white fluorescent light, were preferred as the light environment. The results revealed that LED lights were more effective for heavy metal accumulation, with red LED light significantly enhancing the plant's phytoremediation capacity compared to other LED applications. Moreover, when examining biochemical stress parameters such as levels of photosynthetic pigments, protein concentrations, and lipid peroxidation, plants under red LED light showed better results. Generally, the lowest results were obtained under white fluorescent light. These findings contribute to phytoremediation studies by highlighting the integration of LED lights, thereby enabling the development of a more effective, cost-efficient, and environmentally sustainable remediation system compared to other treatment methods.

Potential Combination of Phytoremediation Plants and Compost in Remediation of Hg in Ex-Gold Mine Soil Contaminated Mercury (Hg)

Heavy metal mercury pollution in the environment can have negative impacts on both surrounding ecosystems and human health. Sources of mercury pollution often come from mining activities, particularly from many unlicensed gold mines (PETI) that use the mercury amalgamation method to extract gold. Due to the persistent, bioaccumulative, and toxic nature of mercury, contamination in the soil has increased. One effort to reduce mercury toxicity is the application of absorption methods using phytoremediation plants and compost as regulating factors. The aim of this study is to review sources of information related to the use of phytoremediation plants and the application of compost as remediators to clean mercury (Hg)-contaminated soil. To reduce levels of heavy metal contamination, plants employ five phytoremediation mechanisms: phytoextraction, phytostabilization, rhizofiltration, phyto degradation, and phytovolatilization. Therefore, selecting hyperaccumulator plants that have the ability to accumulate mercury (Hg) at levels 100 times higher than ordinary plants is crucial for effective remediation. Additionally, the use of compost as a source of organic material can enhance mercury mobility. The addition of organic materials such as compost can increase C organic content, cation exchange capacity (CEC), pH, and Hg-chelate formation, thereby maximizing mercury absorption by plants. This combination has a positive impact on reducing mercury levels in the soil and is environmentally safe.

Arsenic tolerance in Tagetes erecta L.: Phytoaccumulation, physicochemical and anatomical studies through electron microscopy

Arsenic (As), a toxic metalloid that is becoming more and more concentrated in the environment, poses risks to human health, plant production, and species at every trophic level through bioaccumulation and biomagnification. Finding tolerant and hyperaccumulative plant species that can be used in contaminated land is a potential eco-friendly strategy for As removal in terms of its mitigation. Tagetes erecta L. is an ornamental plant species chosen as phytoremediator plant for As removal. To assess the morphological and biochemical changes we conducted pot experiments with plants that were treated with different concentration of sodium arsenate (Na3AsO4) for up to 60 days. Atomic absorption spectrophotometer (AAS) was used to measure the amount of As absorbed in various tissues of the plant. Highest As accumulation was detected in root tissues (12.864 mg kg−1), whereas shoot had least quantity of As (3.443 mg kg−1). With increasing As concentrations, the maximum levels of proline and polyphenol were 14.743 µ mol g−1 and 4.25 mg g−1, respectively. At 60 days, antioxidant enzymes APX (6.952 mM mg−1), CAT (2.143 mM mg−1), and GR (76.631 mM mg−1) were found to be increased. As transport and distribution were confirmed by anatomical analysis in root and shoot tissues by FESEM-EDX, TEM, and light microscopy. The results obtained after morpho-physiological and anatomical studies are in favor of high degree of tolerance of As concentration (25 mg kg−1) by T. erecta suggesting that it could be a potential phytoremediator of As from polluted soil.

Exploring the Potential of Crotalaria juncea L. for Phytoremediation: Insights from Gas Exchange, Pigment Quantification, and Growth Measurements under Copper Stress

Soil contamination by trace elements is a worldwide concern that can result from several sources, such as mining, smelting, car traffic exhaust, agriculture plant protection products such as fungicides, and fertilizers. Among the metals involved, copper can cause alterations in the photosynthetic, respiratory, and enzymatic processes of plants, leading to reduced growth of roots and shoots. An alternative to dealing with metals present in the soil is phytoremediation, which consists of using plants to extract or stabilize these elements. The leguminous Crotalaria juncea is widely used as a green manure and may be advantageous due to its capacity for biological nitrogen fixation and biomass accumulation. This research aimed to evaluate the growth and physiological behaviour of C. juncea in copper-contaminated soil and its potential use as a phytoremediation plant. For the fresh and dry mass of shoots and roots, compared with 30 mg.dm−3 of Cu, there was a decrease in values with the increase in concentrations up to a dose of 480 mg.dm−3. The roots were less sensitive to increased Cu concentrations than the shoots. The tolerance index decreased as copper concentrations in the soil increased. From 60 mg.dm−3, its vegetative growth decreased, but C. Juncea was able to tolerate and accumulate copper in the root system, presenting high potential as a phytostabilizing species.

Synergistic role of phenylpropanoid biosynthesis and citrate cycle pathways in heavy metal detoxification through secretion of organic acids

Excessive heavy metal contaminants in soils have serious ecological and environmental impacts, and affect plant growth and crop yields. Phytoremediation is an environmentally friendly means of lowering heavy metal concentrations in soils. In this study, we analyzed phenotypic and physiological traits, and the transcriptome and metabolome, of sheepgrass (Leymus chinensis) exposed to cadmium (Cd), lead (Pb), or zinc (Zn). Phenotypic and physiological analysis indicated that sheepgrass had strong tolerance to Cd/Pb/Zn. Transcriptomic analysis revealed that phenylpropanoid biosynthesis and organic acid metabolism were enriched among differentially expressed genes, and metabolomic analysis indicated that the citrate cycle was enriched in response to Cd/Pb/Zn exposure. Genes encoding enzymes involved in the phenylpropanoid and citrate cycle pathways were up-regulated under the Cd/Pb/Zn treatments. Organic acids significantly reduced heavy metal accumulation and improved sheepgrass tolerance of heavy metals. The results suggest that synergistic interaction of the phenylpropanoid and citrate cycle pathways in sheepgrass roots induced organic acid secretion to alleviate heavy metal toxicity. A cascade of enzymes involved in the interacting pathways could be targeted in molecular design breeding to enhance phytoremediation.

THE POTENTIAL OF Catharanthus Roseus AS A PHYTOREMEDIATION AGENT FOR HEAVY METAL REMOVAL IN CONTAMINATED SOIL

Heavy metals from human activities, such as industrial emissions and mining waste, contribute to environmental, nutritional, and health issues. Catharanthus roseus, a phytoremediation plant, was tested to determine the levels of heavy metals in soil and plants. The experiment involved preparing various amounts of heavy metals by combining four elements: Cadmium (Cd), Copper (Cu), Lead (Pb), and Zinc (Zn). Four treatment groups were tested, each with varying levels of heavy metal contamination. The initial group (T0) served as the positive control, while the next four groups (T1-T4) were subjected to escalating levels of heavy metals. T1 was given a 200ml solution containing 0.9 (Cd) + 4.0 (Cu) + 0.18 (Pb) + 6.9 (Zn). T2 was given a 200ml solution containing 11.90 (Cd) +19.80 (Cu) +36.00 (Pb) + 54.3 (Zn). T3 was given a 200ml solution containing 23.80 (Cd) + 39.60 (Cu) + 72.00 (Pb) + 108.6 (Zn). T4 was given a 200ml solution containing of 23.80 (Cd) + 39.60 (Cu) + 72.00 (Pb) + 108.6 (Zn). The dataset included measurements of soil pH, heavy metal concentrations, plant height, and shoot and root length. The results showed that the T3 solution, consisting of 23.80 (Cd) + 39.60 (Cu) + 72.00 (Pb) + 108.6 (Zn), had the most favourable plant growth performance based on soil and plant concentrations. This suggests that immediate action is needed to ensure safe food availability.

Role of malic acid in enhancing the efficiency of barley (Hordeum vulgare L.) and alfalfa (Medicago sativa L.) plants for phytoremediation of salt affected soil

Soil salinization is a growing global problem that influences plant growth and crop productivity. Most of the reclamation efforts in the past have focused on the installation of surface drainage systems. Other management approaches, such as excessive leaching and chemical amendments, have also been used on a limited scale to enhance the productivity of these soils. Phytoremediation can be cost-effective and environmentally sound technology.A laboratory experiment was carried out to study the role of malic acid which is low molecular weight organic acid (LMWOA) in enhancing the efficiency of barley and alfalfa plants for the phytoremediation of salt-affected soil. Seeds of barley and alfalfa were cultured in pots and irrigated with full strength Hoagland nutrient solution with three concentrations of seawater (SW) (10%, 20% and 30%) and a mixture of seawater with malic acid (MA) at 2, 4 and 6 mM l-1 (MA+SW), Hoagland solution was used as control. After twelve weeks, plants were harvested, and three types of soils (barley soil, alfalfa soil, and plant-free soil) were subjected to physical and chemical analysis for EC (electrical conductivity), TOC (total organic carbon), pH, potassium, sodium, and chloride ions. Results indicated a significant decrease was recorded in soil EC, pH, potassium, sodium, and chloride ions and a significant increase in soil TOC in barley and alfalfa soil compared with plant-free soil. Treatments with (MA+SW), especially at (2+10%) resulted in a significant increase in ions availability and phytoremediation activity in barley and alfalfa soils comparing with plant-free soil.

Phytoremediation mechanism and role of plant growth promoting rhizobacteria in weed plants for eco-restoration of hazardous industrial waste polluted site: a review.

Plants have numerous strategies for phytoremediation depending upon the characteristic of pollutants. Plant growth promoting rhizobacteria (PGPR) are essential to the process of phytoremediation and play a key part in it. The mechanism of PGPR for phytoremediation is mediated by two methods; under the direct method there is phytohormone production, nitrogen fixation, nutrient mineral solubilization, and siderophore production while the indirect method includes quorum quenching, antibiosis, production of lytic enzyme, biofilm formation, and hydrogen cyanide production. Due to their economic and environmental viability, most researchers have recently concentrated on the potential of weed plants for phytoremediation. Although weed plants are considered unwanted and noxious, they have a high growth rate and adaptability which opens a high scope for its role in phytoremediation of contaminated site. The interaction of plant with rhizobacteria starts from root exudates containing various organic acids and peptides which act as nutrients essential for colonization and siderophore production by the rhizospheric bacteria. The rhizobacteria, while colonizing, tend to promote plant growth and health either directly by providing phytohormones and minerals or indirectly by suppressing growth of possible phytopathogens. Recently, several weed plants have been reported for phytoextraction of heavy metals (Ni, Pb, Zn, Hg, Cd, Cu, As, Fe, and Cr) contaminants from various agro-based industries. These potential native weed plants have high prospect of eco-restoration of polluted site with complex organo-metallic waste for sustainable development.

Reducing Heavy Metal Contamination in Soil and Water Using Phytoremediation.

The increase in industrialization has led to an exponential increase in heavy metal (HM) soil contamination, which poses a serious threat to public health and ecosystem stability. This review emphasizes the urgent need to develop innovative technologies for the environmental remediation of intensive anthropogenic pollution. Phytoremediation is a sustainable and cost-effective approach for the detoxification of contaminated soils using various plant species. This review discusses in detail the basic principles of phytoremediation and emphasizes its ecological advantages over other methods for cleaning contaminated areas and its technical viability. Much attention has been given to the selection of hyperaccumulator plants for phytoremediation that can grow on heavy metal-contaminated soils, and the biochemical mechanisms that allow these plants to isolate, detoxify, and accumulate heavy metals are discussed in detail. The novelty of our study lies in reviewing the mechanisms of plant-microorganism interactions that greatly enhance the efficiency of phytoremediation as well as in discussing genetic modifications that could revolutionize the cleanup of contaminated soils. Moreover, this manuscript discusses potential applications of phytoremediation beyond soil detoxification, including its role in bioenergy production and biodiversity restoration in degraded habitats. This review concludes by listing the serious problems that result from anthropogenic environmental pollution that future generations still need to overcome and suggests promising research directions in which the integration of nano- and biotechnology will play an important role in enhancing the effectiveness of phytoremediation. These contributions are critical for environmental scientists, policy makers, and practitioners seeking to utilize phytoremediation to maintain the ecological stability of the environment and its restoration.

Characteristics Of Physical And Chemical Properties Of Soil After Gold Processing (Case Study Of Unlicensed Gold Mining In The District Sumbawa Regency, Indonesia)

The purpose of this study was to analyze the physical and chemical characteristics of the soil used for unlicensed gold processing in West Sumbawa Regency. This research was carried out in Kuang Village, Taliwang District, West Sumbawa Regency in September 2023. The criteria for soil sampling location is that the activity of the spindle has been inactive for 5 years, using mercury as a material for gold extraction and is in the home environment. This study was conducted by quantitative descriptive method or direct observation in the field and soil analysis in the Laboratory of soil chemistry and Biology, Faculty of Agriculture, University of Mataram. Furthermore, soil sampling was carried out using purposive sampling method or based on purpose with consideration of potential pollution. Based on the results, it can be seen that the physical characteristics of soil including clay with volume weight, specific gravity and porosity are quite dense soil with poor infiltration, while the chemical characteristics of soil include low fertility of both C-organic, CEC, soil pH, N and P soil. The concentration of Hg found in soil ranges from 1.42-15.83 ppm. In this case, mercury contaminated soil needs to be remediated using environmentally friendly materials, cheap and the availability of many in nature. Efforts that can be done is the use of green technology using phytoremediator plants and soil improvement materials, namely biochar. Therefore, it is necessary to conduct further research aimed at reducing the availability and mobility of mercury in the soil

Effectiveness of aquatic plants as phytoremediation in palm oil processing factory wastewater

The research aims to see the effect of using kale (Ipomoea Aquatica), water hyacinth (Eichhornia crassipes) and apu-apu (Pistia statiotes) in the phytoremediation process on water quality in palm oil mill waste. The design used in this research is an experimental design using a non-factorial Completely Randomized Design (CRD). The treatments in the study used 4 treatments and three replications, namely P1: 20 liters of palm oil waste + 600 grams of water spinach, P2: 20 liters of palm oil waste + 600 grams of water hyacinth, P3: 20 liters of palm oil waste + 600 grams of apu-apu, P4: 20 liters of palm oil waste. The observation parameters in this study include pH, DO, Temperature, Nitrate and Phosphate. The results showed that the best aquatic plants in phytoremediation of palm oil wastewater were in treatment B (water hyacinth) and the lowest in treatment D (control). From the results obtained, it can be concluded that water hyacinth is suitable for use as a plant in phytoremediation and is able to improve the quality of palm oil wastewater.

Exogenous of different elicitors: proline and ornithine on Sansevieria trifasciata under particulate matter (PM) and volatile organic compounds (VOC).

Phytoremediation has become famous for removing particulate matter (PM) and volatile organic compounds (VOC) in situ. Plants for removing PM and VOC were associated with botanical biofilters to attract pollution to the plant. On the other hand, persistent pollution exposure can lower plant health and phytoremediation effectiveness; therefore, improving plant tolerance against stress is necessary. Various elicitors can enhance plant tolerance to certain stressors. This study aims to investigate different elicitors to maintain plant health and improve the use of plants in phytoremediation for PM and VOC pollution. This experiment used Sansevieria trifasciata hort. ex Prain under PM and VOC stress. Exogenous elicitors, such as proline, ornithine, and a commercial product, were applied to the leaf parts before exposure to PM and VOC stress. The initial concentrations of PM1, PM2.5, and PM10 were 300-350, 350-450, and 400-500µgm-3, respectively, while the VOC concentration was 2.5-3.0mgm-3. The plant was stressed for 7days. The result indicated that ornithine 10mM is vital in improving plant tolerance and inducing antioxidant enzymes against PM and VOC, while proline 50mM and a commercial product could not reduce plant stress. This study suggests that ornithine might be an important metabolite to improve plant tolerance to PM and VOC.