Industrial hemp is a valuable fibrous plant with high biomass production for it can survive under variable environmental stresses including heavy metals and is used in a wide range of products. This research work aimed at determining the growth potential of industrial hemp (Cannabis sativa) developed on lead (Pb) contaminated mine soils using various chelating agents viz., ethylenediaminedisuccinic acid (EDDS), ethylenediaminetetraacetic acid (EDTA), citric acid (CA) and nitrilotriacetic acid (NTA) under various concentrations of nitrogen (N) fertilizer such as 0 (control), 50, 150, 250 and 350 kg ha−1. Results indicated that the synergistic effect of chelating agents and rising concentrations of N fertilizer resulted in a linear increase in hemp growth and dry biomass. The chelating agent and N mediated showed better response in the photosynthetic, antioxidative, and osmolytic potential of hemp as compared with control. The concentration of N and Pb plants showed a differential response such as N accumulation was maximum in plant leaf and shoot portions as compared to the roots. In contrast, Pb enrichment was higher in the roots than in the plant’s leaves and stems when exposed to varying concentrations of N fertilizer and chelating agents in the soil environment. Biomobilizaiton of Pb in the plant continuum enhanced with the rising N fertilizer levels in the soil. The EDTA showed better phytoextraction potential as compared with the rest of the chelating agents. Hence, findings suggest that the synergistic application of chelating agents and N fertilizer is a far more effective technique that increases the bioavailability of Pb for phytoextraction and decreases Pb-induced oxidative injuries by restricting Pb uptake.
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