Phytochemical Tests Research Articles

Antibacterial Potential of Selected Traditional Medicinal Plants for Wound Healing in Sekela District, Northwestern Ethiopia

ABSTRACT This study evaluated the antibacterial activity of medicinal plants (MPs) used to treat wounds. Methanol and ethyl acetate crude extracts of four medicinal plants were examined for antibacterial efficacy against gram-positive and gram-negative American Type Culture Collection (ATCC) strains. Plant crude extracts were produced using the maceration technique and analyzed using qualitative phytochemical tests. The antibacterial properties of plant products were evaluated using the agar disc diffusion assay. The broth microdilution method was used to determine the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations. The data were analyzed using Analysis of Variance (ANOVA). The methanol extract of Rumex nervosus had the highest yield (21%). The methanol extracts of all tested MPs tested positive for alkaloids, phenolics, and tannins. The methanol and ethyl acetate extracts exhibited a dose-dependent increase in the growth inhibition zone against all ATCC. The methanol extract of Plantago lanceolata demonstrated the highest antibacterial activity (16.67 ± 1.15) against Staphylococcus epidermidis. S. aureus and S. epidermidis recorded a relatively lower MIC (6.25 mg mL−1) from methanol extracts of P. lanceolata, while P. lanceolata and R. nervosus recorded a lower MBC (25 mg mL−1) against S. aureus.

Pharmacognostic Characterization of Primula macrophylla by Light Microscopy, Scanning Electron Microscopy and Analytical Techniques.

Traditional medicinal systems have extensively used Primula macrophylla (Primulaceae) to treat a variety conditions, including bronchitis, asthma, joint pain, fever and so forth. This study determines various pharmacognostic and phytochemical standards helpful to ensure the purity, safety, and efficacy of medicinal plant P. macrophylla. In experimental section the Intact aerial parts, powdered materials, and extracts were examined macro- and microscopically and pharmacognostic standardization parameters were determined in accordance with the guidelines given by the World Health Organization. Parameters including extractive values, ash values, and loss on drying were determined. Preliminary phytochemical tests, fluorescence analysis, and chromatographic profiling were performed for the identification and standardization of P. macrophylla. The results of macroscopic studies revealed that leaves are farinose, alternating, foliage, toothed and flowers are unbranched, hermaphroditic, large, herbaceous, round, non-woody stem, roots are adventitious. Length of leave is 11 cm, flower 3 cm, stem 38 cm and root 18 cm. Odor is clove or Eucalyptus like, irritating smell and has pungent/ bitterness. Qualitative analysis confirmed that carbohydrates are not present and saponins, terpenoid, flavonoid, phenolic, phytosterol and fixed oils are not present. The atomic absorption spectrophotometer (AAS) showed the trace amounts of Pb (1.604 mg/L), As (-12.91 mg/L), Hg (7.102 mg/L), and Cd (0.226 mg/L) present. The internal structure of the plant was examined using light microscopy (LM) and scanning electron microscopy (SEM). The plant cross-sectional scans revealed several useful botanical properties. LM and SEM revealed important diagnostic features including stomata, phloem, xylem, spiral vessels, and trichomes and so forth. We conclude that the data generated from the present study help to authenticate the medicinally important plant P. macrophylla. This study is helpful for establishing the pharmacopeia standards in accordance with WHO guidelines.

Phytochemical and Biochemichal Profile of Raw Extracts and Oil from Curcuma longa Rhyzomes Grown in Diabo (Côte d'Ivoire)

Aims: The aim of this study was to determine the phytochemical and biochemichal profile of Curcuma longa grown in Diabo, central Côte d'Ivoire. Place and Duration of Study: Department of Biochemistry-Genetics, Unit for Training and Research in Biological Sciences, Peleforo University GON COULIBALY Korhogo, BP 1328 Korhogo; The first part of this study was carried out from April 1, 2023 to December 31, 2023; the second part was conducted from March 01, 2024 to July 31, 2024. Methodology: Several phytochemical tests based on colorimetric reactions were used to perform qualitative and quantitative analyses of polyphenols, flavonoids and tannins. To characterize the oil and crude extracts, the indices of iodine and refractive as well as pH and Brix were determined. Results: The results show the presence of several secondary metabolites in the crude extracts, including polyphenols (53.1 ± 2.08 mg Eq AG/g extract), flavonoids (2.7 ± 0.22 mg Eq AG/g) and catechic tannins (9.1 ± 0.7 mg Eq C/g) in variable quantities. Biochemical analyses showed a pH close to neutral (pH = 6.68). The Brix value (23.20 ± 0.26 min), shows that Curcuma longa extracts, in particular EHCL, contain several soluble solids such as reducing sugars, acids, salts, pectins and nitrogenous matter. As far as the oil is concerned, the iodine value obtained was 84.1 ± 0.1 g I2 /100 g fat; this high value is close to 100 g. The refractive index was 1.52 ± 0.61. Conclusion: Phytochemical and biochemical characteristics show that Curcuma longa rhizomes could have interesting pharmacological potential.

EXTRACTION, Extraction, Isolation, Identification and Estimation of Diosgenin by TLC Profiling and UHPLC-LC-SRM Analysis in Three Costus Species

Medicinal plants are utilized for treating many ailments, since ancient times due to their tremendous therapeutic properties. The plant derivatives are used as a raw material for the synthesis of drugs either as a natural or artificial synthetic drug. Costaceae family members are known to possess many medicinal properties few of them are mainly consumed to reduce the blood glucose level and treat many disorders. The sapogenin contained in the rhizome of three Costus species was extracted, identified, and characterized. Young rhizomes were harvested surface sterilized, hydrolyzed; extracted through a soxhlet apparatus and concentrated fractions were subjected to a preliminary phytochemical screening test for saponin, Thin Layer Chromatography, and the UHPLC-MS SRM analysis. The result showed that the maximum yield of the Diosgenin was obtained from the chloroform extract of Costus speciosus. The pharmaceutical analysis in all the three Costus species carried out with isolated saponin was compared with standard Diosgenin. The characterization of isolated diosgenin was done by UHPLC MS and SRM, based on the retention time of all three samples and the comparison with standard Diosgenin. Isolated Diosgenin was quantified through thin layer chromatography with n-hexane: ethyl acetate (7:3) as mobile phase at Rf value of 0.48. The amount of Isolated Diosgenin was estimated by comparing the peak area at a retention time Rt value of 8.5 in all the samples and compared with standard Diosgenin high amount of Diosgenin was found in Costus speciosus rhizome followed by Costus igneus and Costus pictus. The preliminary phytochemical screening and analytical methods involved during the present study were found satisfactory. Thus, the protocol can be used for the extraction of Diosgenin and further used in the synthesis of Drugs. Keywords: Costus pictus, Costus speciosus, Costus igneus, rhizome, TLC, UHPLC-MS, SRM

Anticancer Potential Of Psoralea Corylifolia L. Ethanol Extract: Cytotoxicity, Apoptosis Mechanisms, And Gene Expression Analysis In Mcf7 Breast Cancer Cells Via qPCR

Aim: The aim of this study was to evaluate the phytochemical profile and anticancer potential of Psoralea corylifolia L. extract, specifically focusing on its effects on breast cancer cells (MCF7) and its underlying molecular mechanisms. Methods: The seeds of P. corylifolia were extracted using ethanol and chloroform through Soxhlet extraction. The extracts were analyzed for bioactive compounds through standard phytochemical tests. The anticancer activity of the ethanol extract was assessed using the MTT assay to determine cell viability and IC50 values. Further molecular analysis of apoptosis was performed by quantifying the expression of the Bcl-2 gene using semi-quantitative PCR and evaluating marker of apoptosis. Results: Phytochemical screening revealed that both ethanol and chloroform extracts contained alkaloids, carbohydrates, and terpenoids, with ethanol extract showing a broader range of bioactive compounds, including flavonoids, phenols, saponins, and proteins. The ethanol extract exhibited significant cytotoxicity against MCF7 cells, with an IC50 value of 10.1 µg/ml. Molecular analysis showed that the extract induced apoptosis through down-regulation of the anti-apoptotic protein Bcl-2 and activation of caspase-3 and PARP, suggesting the involvement of the mitochondrial pathway. Conclusion: The results demonstrate the anticancer potential of P. corylifolia ethanol extract against breast cancer cells, primarily through cell cycle arrest and apoptosis induction. These findings highlight P. corylifolia as a promising candidate for further investigation as a safer, plant-based alternative in cancer treatment, particularly for breast cancer, though clinical studies are needed to confirm its therapeutic efficacy in humans.

In-vitro Antioxidant, and In-vivo Analgesics and Anti-inflammatory Activity of Allamanda blanchetii Leaf Extract in Rats.

This study assessed the In-vitro Antioxidant and In-vivo Analgesics and Anti-inflammatory Activity of Allamanda blanchetii Leaf Extract in Rats. Diverse pharmacological applications of plants from the Apocynaceae family are reported in the literature. Allamanda blanchetii; an ornamental species belonging to the Apocynaceae family, is characterized by diverse biological activities, i.e. antioxidant, cyto-toxic, thrombolytic, membrane-stabilizing, antimicrobial, and anti-proliferative effects. This species represents a perennial flora that thrives in tropical and subtropical climates. Ultrasonication-assisted method used for plant extraction. The ex-tracts were subjected to phytochemical screening tests, followed by total phenolic content analysis, using gallic acid as a standard. The antioxidant activity was examined by DPPH scavenging and FRAP assays. The acetic acid-induced writhing test, tail flick, and Hot plate method were used for the determination of analgesic activity. Anti-inflammatory activity by following carrageenan-induced paw edema Results: ABLE treatments show analgesic effectiveness against the acid-induced pain source, tail flick, and hot plate methods at different doses of ABLE 400,200,100 mg/kg results showed respectively- 55.32, 38.67, and 22.85 (% inhibition), 89.47%, 62.57 %, 49.57%, and 100%, 92.40%, 65.33% response after 180 min of drug administration. ABLE 400 and 200 mg/kg exhibit effective results (1.43± 0.005 and 1.50± 0.008) against carra-geenan-induced intoxication. The fundamental components of antioxidants that can aid in the reduction of free radicals include phenol, flavonoids, and polyphenols. Applying DPPH and FRAP, ABLE exhibits remarkable antioxidant activity. When it comes to both centrally and periph-erally acting analgesics, ABLE exhibits highly effective activity. Methanolic ABLE had a noticeable impact on paw edema caused by carrageenan. Antioxidants, alkaloids, and gly-cosides were present in methanolic ABLE, which allowed it to efficiently combat inflam-matory mediators and the cause of pain. Ultrasonic assistance is beneficial in isolating active metabolites in plants, with phenolic compounds exhibiting antioxidant activity. ABLE, a plant with 55% squalene, ef-fectively combats inflammatory mediators and pain. Further investigation is needed to iden-tify biomarkers in the plant.

Antioxidant Activity of Hotong (Setaria italica) Seeds from Buru and its Implications for Biochemistry Learning

Hotong Buru seeds (Setaria italica) are a local wisdom food ingredient in South Buru Regency, Maluku Province, Indonesia. These hotong seeds are widely consumed by the people of South Buru as a staple food substitute for rice. Hotong Buru seeds contain bioactive components that have antioxidant properties, including tannins and Vitamin E. Tannins are polyphenols, one of the anti-nutrients found in food ingredients. These components are mostly contained in the epidermis. This research aims to analyze the antioxidant activity of Hotong Buru (Setaria italica) seeds and their implication for Biochemistry Learning. Antioxidant analysis was conducted using 1,1-dephenyl-2-picryl-hydrazyl (DPPH) as a free radical. Descriptive analysis was used to describe the chemical composition and quality of Hotong Buru seeds in tables and graphs. Inferential analysis was done using one way ANOVA technique to analyze the effect of food quality. All data were presented descriptively, and antioxidant activity was calculated using the SPSS Program. The results showed that Hotong seeds from Buru Island positively contain secondary metabolite compounds such as alkaloids, flavonoids, terpenoids, steroids, tannins, and saponins. The content of these secondary metabolites can act as antioxidants for the human body. Antioxidants are very important to delay or inhibit damage to molecular compounds. In addition, the results of this study are implicated in biology learning, especially biochemistry, food science, and nutrition courses. The phytochemical test showed that there were antioxidants in Hotong Buru seeds ingredients, there are alkaloids, flavonoids, steroids, tannins, terpenoids, and saponins. The implications of research results for biology learning have been made clear in the form of practical instructions that have been applied to students of the Pattimura University Biology Education Study Program. Keywords: Hotong buru, antioxidants, culinary ingredients, biochemistry learning

Comparative Anatomical, Histochemical, and Phytochemical Characterization of Species of the Genus Cucumis L.

Cucumis anguria L. and Cucumis dipsaceus Ehrenb. ex Spach belong to the Cucurbitaceae family and are popularly known as "maxixe." In folk medicine, they are used to treat pneumonia, hyperglycemia, wounds, and malaria. This study aims to characterize the anatomy, histochemistry, and phytochemistry of C. anguria and C. dipsaceus. Conventional methods in plant anatomy and microscopy were used to prepare and analyze semi-permanent slides containing cross-sections of the stem, petiole, leaves, and paradermal sections of the leaf blade. For the histochemical analysis, different reagents were used according to the target metabolite. Phytochemical tests of methanolic extracts of leaves were performed by thin-layer chromatography. The microscopic analysis allowed obtaining the differentiation of plant cells, the presence of tector and glandular trichomes, in addition to showing anatomical traits such as stem shape, petiole shape, and organization of the mesophyll. Histochemistry showed the presence of alkaloids, starch, phenolic compounds, lipophilic compounds, lignin, and tannins. Phytochemical prospection identified monoterpenes, sesquiterpenes, triterpenes, steroids, alkaloids, and reducing sugars. The obtained results provide important information for quality control and species differentiation since there are few studies in the literature on these species.

Phytochemical Screening of Seaweed Flour (Eucheuma cottonii) Using Various Organic Solvents and Its Application in Tilapia Feed (Oreochromis niloticus)

This study aims to qualitatively analyze the bioactive compounds in Eucheuma cottonii seaweed flour extract and its application in commercial feed for tilapia (Oreochromis niloticus). The methodology employed is experimental and consists of two phases. Phase 1 involves the extraction of E. cottonii flour using three different organic solvents with varying polarity (ethanol, ethyl acetate, and a combination of ethanol + ethyl acetate). The testing parameters include qualitative phytochemical tests for alkaloids, steroids/triterpenoids, saponins, flavonoids, and tannins. Phase 2 consists of the application of the extract to tilapia over a 30-day rearing period with four treatments: control/commercial feed (P0), commercial feed + ethanol extract (P1), commercial feed + ethyl acetate extract (P2), and commercial feed + combined ethanol and ethyl acetate extract (P3). The parameters tested include absolute weight, specific growth rate, survival rate, and water quality. Phytochemical and water quality data are presented in table format. Meanwhile, growth and survival data are analyzed using ANOVA, followed by Duncan’s test for significant differences. The results of the researches carried out, it can be concluded that the phytochemical analysis of E. cottonii extract in ethanol and the ethanol + ethyl acetate combination yielded similar compounds: alkaloids, steroids/triterpenoids, and flavonoids. The ethyl acetate extract contained triterpenoids and flavonoids. Both saponins and tannins returned negative results across all organic solvent treatments. All treatments with the addition of E. cottonii seaweed extract in various organic solvents demonstrated better growth in tilapia compared to the control treatment.

Phytochemical Analysis and Toxicity Test of Secondary Metabolite Compounds in Methanol Extract from Tuber of Belajang Keissi’ (Stephania venosa (Blume) Spreng)

The tuber of Stephania venosa (Blume) Spreng, locally known as Belajang Keissi’, belongs to the Menispermaceae family and is traditionally used in Indonesia for treating diabetes mellitus, as a nerve tonic, and as an anti-cancer remedy. This plant is recognized for its bioactive compounds with anti-inflammatory, analgesic, antioxidant, and anti-tumor properties. The objective of this study was to analyze the phytochemical content and evaluate the toxicity of the methanol extract of Stephania venosa tubers to explore its potential as an anticancer agent. The study involved sample preparation, extraction by maceration, phytochemical group testing using specific reagents, and toxicity assessment through the Brine Shrimp Lethality Test (BSLT) method. Phytochemical screening revealed the presence of alkaloids and flavonoids as the major secondary metabolites, while the toxicity test showed that the extract exhibited significant lethality to Artemia salina larvae, with an LC50 value of 14.92 ppm. This result indicates high toxicity, meeting the threshold of bioactivity for potential therapeutic applications (LC50 ≤ 1000 ppm). The mechanism of toxicity is hypothesized to involve the interference of alkaloids with the neural and digestive systems of the larvae. This study highlights the potential of Stephania venosa tubers as a source of bioactive compounds for antibacterial, antifungal, or anticancer development, warranting further isolation and advanced pharmacological testing to confirm its therapeutic applications.

Synergistic Phytochemical and Nanotechnological Exploration of Melia azedarach With Silver Nitrate: Elucidating Multifaceted Antimicrobial, Antioxidant, Antidiabetic, and Insecticidal Potentials.

Plants are a rich source of bioactive compounds with significant pharmaceutical and health applications. This study explores the phytochemical, therapeutic, and phytotoxic properties of Melia azedarach by analyzing extracts from its bark, flowers, leaves, and fruits using six solvents: ethanol, methanol, acetone, hexane, chloroform, and distilled water. Twenty-one phytochemical tests were conducted, revealing significantly positive results for various tests. However, the ethanolic and methanolic flower extracts yielded no significant results in other tests. The highest total phenolic content was found in the chloroform extract of the leaves (96 ± 0.01 mg/100 g), and the highest antioxidant activity was observed in the ethanolic and hexane leaf extracts, with a 98% DPPH scavenging rate. Antibacterial testing showed significant efficacy against Serratia marcescens, Bacillus subtilis, Kluyvera spp., and Pseudomonas spp., with p values < 0.0001. The fruit chloroform extract demonstrated the highest alpha-amylase inhibition (93 ± 0.05), while the ethanolic leaf extract had the greatest tumor inhibition (85.6 ± 0.5). Insecticidal assays revealed that the acetone bark extract had the highest control values (56% and 57%). Due to their higher reducing potential, the leaves were used to biosynthesize silver nanoparticles (AgNPs), characterized by UV-Vis spectroscopy, EDX, and SEM, revealing an average particle size of 20-30 nm and spherical morphology. The AgNPs exhibited excellent antibacterial, antioxidant, antidiabetic, and insecticidal activities. These findings highlight the potential of M. azedarach and its AgNPs for developing novel therapeutic agents.

Phytochemical analysis of stem and leaves extracts of Tinospora cordifolia (Thunb.) Miers

Tinospora cordifolia (Thunb.) Miers is a large deciduous, creeping shrub belonging to the family Menispermaceae. The phytochemical analysis of the plants is very important commercially and has great interest in pharmaceutical companies for the production of the new drugs for curing of various diseases. The present study involves the qualitative phytochemical analysis of the selected medicinal plants. The aim of this study is carried out to analyze the phytochemical compounds in stem and leaves extracts of selected plant by using phytochemical screening tests. The leaf and stem extracts of the plant is expressed the presence of several phytochemicals viz., flavonoids, amino acids, diterpines, protein, saponins and carbohydrates. The result of phytochemical screening tests revealed that diterpines and carbohydrates are positive in all extracts. But flavonoids and saponins only present in methanol and ethanol extracts. The studies justify that T. cordifolia use in traditional medicines. The investigation further proposed that the phytochemicals present in stems and leaves of the plant, which can be used as natural antioxidants in medicinal drugs.

Phytochemical characterization, antioxidant activity and anthelmintic effect of Carica papaya L. (Caricaceae) seeds in Lohman Brown cockerels

Helminths are diseases which exist frequently with the poultry. To cure these parasitosis, breeders use very often synthetic products which are source of food poisoning for the consumers. However, past studies showed that some plants are known to their anthelmintic properties. For sanitary biosafety, the current study aimed to gauge helminthic capacity of papaya seed and established the relationship between phytochemical composition and anthelminthic activity. Qualitative and quantitative phytochemical analyses were carried out to identify phytoconstituents endowed with anthelmintic activity. Bioguided analyses were carried out in three months upon 260 Lohmann Brown cockerels which were divided in 5 batches: T-, T+, GP1, GP2, and GP. Cockerels of batches GP1, GP2 and GP3 received in their food papaya seed powder (5%) per month, respectively for one, two and three days. Batch T- did not receive any treatment but cockerels of batch T+ were treated by Levamisole (25%) on day per month. Phytochemical tests revealed the presence of alkaloids, reducing compounds, flavonoids, tannins, proteins and coumarins as chemical group in the seeds. Bioguided analyses proved that the reduction rates of number of EPG in batches T+, GP1, GP2, et GP3 are respectively: 90.08; 28.00; 53.00, and 90.35, but it was negative in batch T-. Batches T+ and GP3 presented higher average weekly gains, while average cumulative weight gains were identical for batches T+ and GP3. Nevertheless, it was batch GP3 that provided the lowest consumption index. In fact, cockerel treatment with papaya seed present almost the same anthelminthic efficacy as Levamisole.

Extraction and evaluation for anti-diabetic effects of some Indian plant extracts

Diabetes is a metabolic disease associated with changes in the structure and function of several biological systems. The main cause of tissue injury is persistent hyperglycaemia. The past 20 years have seen increased expansion of the harmful effects of persistent hyperglycaemia, even if peripheral neuropathy and small and large vascular disease can also be used to explain diabetes-related organ failure. Liophyllum decastes, Butea monosperma, and Opuntia dillenil fruit extracts were shown to pass phytochemical testing. The study findings of Butea Monosperma, Opuntia dillenil, and Liophyllum decastes distilled (150 mg/kg) and (300 mg/kg) on STZ-tempted DM rats were paired with Glibenclamide. The glucometer method, serum and urine analysis, as well as factors including body weight, food consumption, glucose levels, and biochemical analysis, were used to determine the blood sugar rankings. Liophyllum decastes fungus fruit, Opuntia dillenil Planned fruit, and distilled Butea monosperma leaves all have anti-diabetic properties for diabetics.

Anti-Mitotic Activity Of Gumamela (Hibiscus Rosa-Sinensis) Leaf Extract In The Early In-Vitro Development Of Sea Urchin (Tripneustes Gratilla) Embryo

Plant-derived products have played an important role in the development of several clinically useful anticancer agents. Several cancer treatments have been widely used, including surgery, chemotherapy, and radiotherapy. However, no selective cancer therapies exist that can eliminate cancer cells while preserving healthy cells unharmed. This study aims to determine the antimitotic activity of Hibiscus rosa-sinensis leaf extract in the early in-vitro development of sea urchin Tripneustes gratilla embryo. The Hibiscus rosa-sinensis leaves were extracted using ethanol into four different concentrations (0.5%, 1.0%, 1.5%, 2.0%). A Sea urchin bioassay was then performed to determine its antimitotic activity against the Tripneustes gratilla embryo. Phytochemical confirmatory tests were also done to confirm the presence of bioactive compounds. The results of the study showed that Hibiscus rosa-sinensis leaf extracts contained alkaloids, flavonoids, tannins, and saponins. The Sea Urchin Bioassay reveals that the various leaf extract concentrations (0.5%, 1.0%, 1.5%, 2.0%) inhibited antimitotic activity against the early cell development of sea urchin embryo as it had a slower rate of mitotic activity as compared to the negative control. Moreover, the higher concentration of leaf extract 1.5% and 2.0% revealed to have greater antimitotic activity similar to that of the positive control colchicine as data showed it had the least number of embryos developed. This implies that the antimitotic activity of Hibiscus rosa-sinensis leaf extracts is concentration-dependent. An ANOVA test showed that there are significant differences between the various leaf concentrations and control groups in the early cell development of sea urchin embryos in minute intervals.

Characterization of Bioactive Compounds and Stability of Mangrove Extract Rhizopora Sp.

In recent decades, bioactive compounds contained in plants have increasingly attracted the attention of researchers. This is because these bioactive compounds have the potential to be used as raw materials in the manufacture of health products, medicines, and cosmetics. However, in developing products based on bioactive compounds in Rhizopora sp. mangroves, sufficient knowledge about these compounds is needed. The main objective of this study was to identify bioactive compounds and evaluate the stability of Rhizopora sp. mangrove extract obtained from Youtefa Bay area, Jayapura City. The methods used include mangrove leaf sampling, maceration extraction using methanol, followed by fractionation and phytochemical tests to detect the content of active compounds. Data analysis was performed using UV and IR spectroscopy. The results showed that the isolated Rhizophora sp. mangrove leaf extract obtained from Youtefa Bay waters contained several bioactive compound activities such as alkaloid, flavonoid, phenolic, and saponin bioactive compound groups in methanol solvents. In conclusion, mangrove Rhizophora sp. from Youtefa Bay has great potential as a source of bioactive compounds that can be further developed in various commercial products.

In Vitro Antioxidant and Antifungal Activities of Extracts from Ocimum basilicum Leaves Validated by Molecular Docking and ADMET Analysis.

The aim of study is to investigate the various mechanism-based antioxidant and anti-fungal properties of a hydroalcoholic extract of Ocimum basilicum L leaves. Additionally, conduct molecular docking to simultaneously validate in vitro activities. Also, perform ADMET analysis to know pharmacokinetic properties and its toxicity for its safety. Prior extract's qualitative analysis has been performed to identify the bioactive compounds by phytochemical tests and GC-MS analysis. Different mechanism-based in vitro antioxidant methods are studied; in different methods, different IC50 values have come, which revealed the extract's antioxidant potentials. The antifungal potential of the extract has been observed by performing a modified poison food assay and a time-killing curve assay. In silico analysis with the human peroxiredoxin 5 enzyme (PDB ID: 1HD2) and secreted aspartic proteinase (PDB ID: 2QZX), which predict extract biological activity, has shown promising results of Ocimum basilicum L extract. In silico findings confirm the in vitro experimental outcome of the extract. The different IC50 values of extracts in different mechanisms indicate their therapeutic potential, and that encourages further research in formulation development. The time-killing assay method gives information about a dynamic interaction between extract and microbial strain. Concentration-dependent antifungal studies have significance in formulation development for dose determination.

Uji Potensi Antituberkulosis Ekstrak N-Heksan dan Ekstrak Etanol Daun Jeruk Nipis (Citrus Aurantiifolia) secara in Vitro

Tuberculosis is a disease caused by Mycobacterium tuberculosis which is widely suffered and causes death. Currently, antituberculosis treatment using synthetic chemicals has been widely resistant. The purpose of this study was to test the potential of lime extract to inhibit the growth of tuberculosis bacteria. This research method uses a quantitative approach and is experimental. The results of the study showed that the results of the phytochemical screening test showed the same group of chemical compounds in fresh leaves, simplex and ethanol extract of lime leaves, namely alkaloids, flavonoids, tannins, saponins, steroids and glycosides. While the n-hexane extract only contains steroids and glycosides. Then the effectiveness of antituberculosis can be seen from the ethanol extract being stronger than the n-hexane extract, namely in the 1st week there was inhibition while the n-hexane extract from the 1st week to the 4th week had bacterial growth.

Hypoglycemic and antioxidant activities of Jasminum officinale L. with identification and characterization of phytocompounds

The utilization of plant-derived chemicals with anti-diabetic properties is widely promoted for its advantageous tactics in managing diabetes, as they are cost-effective and have minimal or no adverse effects. Therefore, this work investigates the medicinal plant Jasminum officinale L. leaves by extraction and bio-guided fractionation. The ethyl acetate fraction showed a higher yield of 36.4 %. A phytochemical test on Jasminum officinale confirmed flavonoids, saponins, phenols, and tannins. The highest total phenol and flavonoid contents in the ethyl acetate fraction of J. officinale are 103.01 ± 1.1 mg GAE/g and 80.29 ± 1.03 mg QUE/ value found in methanol crude extract. Furthermore, HPTLC analysis of the ethyl acetate fraction detected the existence of flavonoids (kaempferol) and phenols (gallic acid, quercetin, and rutin). The compounds detected at the greatest concentrations in the LC-M/MS analysis of the ethyl acetate fraction were cirsiliol, kaempferol, and 2-tridecanone. Additionally, J. officinale (IC50 33.845 ± 1.09 μg/mL) demonstrated the highest DPPH scavenging activity in EAF like that of ascorbic acid (IC50 22.27 ± 0.96 μg/mL). Also, in the FRAP assay, the IC50 of this fraction is 15.14 ± 0.25 μM Fe equivalents. In the range of alpha-amylase deactivating action, from 13.25 % to 74.51 %, and IC50 value (47.40 ± 0.29 μg/mL) was significantly higher in the ethyl acetate fraction of J. officinale leaf extract. Moreover, J. officinale leaf extract had a substantially higher retention of glucose level (23.92 ± 0.85 % to 87.21 ± 0.6 %), significantly higher anti-inflammatory activity with the lowest IC50 value (66.00 ± 1.84), and lipid peroxidation (IC50 value 34.67 ± 1.69) by utilizing egg yolk as a substrate for lipids. Overall, the study revealed that J. officinale has considerable anti-diabetic characteristics. However, further comprehensive research is necessary to ascertain the medicinal purposes of J. officinale and its chemical components, pharmacological effects, and clinical uses.

Utilization of Local Wisdom of Antanan (Centella Asiatica) and Badotan (Ageratum Conyzoides L.) Plants for Anti-bacterial Edible Film of Pumpkin Seeds

Edible film is a thin layer made from consumable materials, formed on top of or placed between food components, functioning as a barrier to mass transfer such as moisture, oxygen, and solutes. It is made from edible biopolymers and food-grade additives. The development of edible film packaging in this study was carried out by utilizing pumpkin skin and seeds that have not been adequately used and by exploring the addition of natural antimicrobial compounds, namely antanan and badotan, to enhance the inhibitory effect of edible films made from pumpkin seeds that meet food standards.In this study, the extraction of yellow pumpkin seeds, antanan, and badotan was performed, followed by organoleptic and phytochemical tests. An edible film formulation was made from yellow pumpkin seeds, varied with antanan and badotan, and tested for moisture content, solubility, pH, and antibacterial properties. The characteristic test results showed that the badotan leaf simplicia was dark green, with a bitter taste and an astringent aromatic odor typical of badotan. The extract was blackish-green in color, with an aromatic odor typical of badotan, and a very bitter taste. Antanan leaf simplicia had a typical aromatic odor, an astringent taste, and was solid green in color. The antanan leaf extract had a typical antanan odor, a very bitter taste, and was thick green in color. Pumpkin seed simplicia had a typical pumpkin smell, an astringent taste, and was thick green in color. The pumpkin seed extract had a smell of ethanol solvent, a bitter taste, and was brownish-green and in the form of a thick liquid. The potential bioactive compounds in badotan leaves included alkaloids, steroids, phenolics, and saponins. Antanan leaves and pumpkin seeds contained flavonoids, alkaloids, steroids, phenolics, and saponins. The results showed that the edible film formulation of pumpkin seeds combined with badotan and antanan (1:2) appeared tighter and smoother and did not break. The formulation of pumpkin seeds and badotan (1:2) had a moisture content of 0.0380%, a solubility content of 0.2075%, and a pH of 5.8. The pumpkin seed and antanan (1:2) formulation had a moisture content of 0.0732%, a solubility content of 5.7269%, and a pH of 5.8. The edible film antibacterial activity test against Staphylococcus aureus used the disc diffusion method and obtained an inhibition zone of 1.8 mm for pumpkin seed edible film, 12.4 mm for pumpkin seed (1:2) edible film, and 17.6 mm for pumpkin seed (1:2) edible film.