Physiological Quality Of Soybean Seeds Research Articles

Quality assessment of soybean seeds submitted to industrial seed treatment and stored in a natural and controlled environment

Seed quality is a limiting factor in the search for high soybean yields. However, fungi and insect pests can negatively interfere with their performance, and industrial seed treatment (IST) is a tool used to minimize the attack of pathogens and pests. The treated seeds are often stored until sowing, which can lead to a reduction in their quality. This work aimed to evaluate the physical and physiological quality of soybean seeds submitted to IST, with subsequent storage in a natural and controlled environment. The experiment was conducted in a split-split-plot design in a completely randomized scheme (time x storage systems x IST) with four replications. Soybean cultivar 55I57RSF IPRO was subjected to IST with fungicide (metalaxyl-M, thiabendazole, and fludioxonil) and insecticide (thiamethoxam and cyantraniliprole), combined or not with polymer and dry powder, with subsequent storage in a controlled and natural environment. The moisture content, thousand-seed weights, germination, first germination count, germination speed index, accelerated aging, seedling length, dry matter transfer, and emergence sand test were evaluated. IST did not affect the moisture content and weight of a thousand seeds. However, storage in a controlled environment provided a greater reduction in moisture content and a smaller reduction in the weight of a thousand seeds. IST negatively affected germination, first germination count, germination speed index, and accelerated aging test. IST negatively affected plant length, except in the treatment where only insecticide was applied. The dry mass transfer was negatively affected but with less intensity in seeds subjected only to the application of insecticide or fungicide and the combination of insecticide, fungicide, and polymer. Seed emergence in the sand was negatively affected by all treatments. Regardless of the treatments, all responses evaluated showed a reduction throughout storage, with greater intensity in seeds stored in the natural environment.

Physical and physiological quality of soybean seeds processed in a static spiral separator

Physical and physiological quality of soybean seeds processed in a static spiral separator

Physiological quality of soybean seeds produced under different spatial arrangements of plants

The present study aimed to evaluate the physiological effect of seeds on soybean genotypes in the southern region of Rio Grande do Sul. The test was developed in the field, in two agricultural crops, on a farm located in the District of Monte Bonito - Pelotas (RS, Brazil), in eutrophic haplic planosol soil, with geographic coordinates 31° 40' 27"S and 52°23' 28 "W. The experiment consisted of twelve treatments involving two factors: factor A - 3 soybean genotypes (NA 5909 RR (super early, growth indeterminate and maturation group A), NS 6006 IPRO (high grain weight, growth indeterminate and maturation group B 5.9), and NS 5959 IPRO (precocity, indeterminate growth and maturation group C) and factor B - 4 line spacing (0.17; 0.30; 0.45 and 0.60 meters). The experimental design used was randomized blocks in a factorial scheme (3x4) with four blocks. The plant population used was 330.000 plants ha-1. The evaluations of the physiological performance of seeds were germination, first germination count, length and dry matter of aerial part, root, and a total of seedlings, accelerated aging, and emergence in the field. Soybean plants better distributed in the cultivation area, in spacings of 0.17 and 0.30 meters, present greater plant height, stem diameter, and productive performance.the only genotype that presented a reduction in germination as the row spacing increased was 'NS 6006 IPRO', resulting in seeds with lower germination in the spacings of 0.45 and 0.60 m compared to the other genotypes under study. The reduction in the spacings, 0.17 and 0.30 meters, produced seeds with better physiological quality, evidenced in years with rain preceding the harvest.

The role of the biochemical composition of soybean seeds in the tolerance to deterioration under natural and artificial aging

The fast decline in the physiological quality of seeds during storage is a serious problem. It is known that the reduction of seed quality may be related to its biochemical constitution. However, the relationship between the composition and the mechanisms linked to the loss of vigor of soybean seeds during aging has not been elucidated yet. Thus, the aim of this work was to analyze the role of the biochemical composition of soybean seeds in the physiological quality and in the tolerance to deterioration due to natural and artificial aging. Seeds of six soybean genotypes were analyzed initially and after being submitted to natural aging, storage for eight months, and artificial aging, using the temperature of 41 °C and 100 % relative humidity for 96 h. Moisture content, germination and vigor tests were carried out. Also, there were measured the content of oil, total protein, soluble protein, malonaldehyde, and fatty acids palmitic, stearic, oleic, linoleic, and linolenic. It was verified that the physiological quality of soybean seeds decreased with both kinds of aging. However, the deterioration process occurs by distinct mechanisms. The biochemical composition of the seeds is associated with the physiological quality and their storage potential is changed by natural and artificial aging. The tolerance of the seed to deterioration is related to soluble protein and fatty acids content. Oleic fatty acid and soluble protein can be used as indicators of physiological quality in soybean seeds.

Physiological quality of soybean seeds as a function of organic-mineral fertilizers

The objective of this work was to evaluate the physiological and sanitary quality of soybean cultivars as a function of doses and sources of organomineral fertilizers. The experimental design was randomized blocks arranged in a split-split plot, with three replications. In the main plots, the treatments consisted of two soybean cultivars (BRASMAX Bônus IPRO and BRASMAX Olimpo IPRO), and in the subplot, the treatments consisted of three phosphate fertilizers [two organomineral fertilizers manufactured and a phosphate fertilizer (simple superphosphate)]. In the subplot, doses of 0 (control), 50 and 100 kg of P2O5 per ha-1 were used. The following parameters: moisture content, emergence, total dry weight, germination, accelerated aging, electrical conductivity and seed sanity were used in current investigation. The use of organomineral fertilizers resulted in positive effects on the physiological quality of soybean seeds, although the incidence of the pathogens Cercospora sp., Aspergillus niger, and Rizoctonia sp. were larger. The combinations of the factors “BRASMAX Bônus IPRO + 50 kg of P2O5 per ha-1 + ORG1 or ORG2” and “BRASMAX Olimpo IPRO + 100 kg of P2O5 per ha-1 + ORG2” were associated with greater physiological and sanitary quality of the seeds of soybean.

Does Forced Plant Maturation by Applying Herbicide with Desiccant Action Influence Seed Longevity in Soybean?

Herbicides with desiccant actions may be used to anticipate the harvesting of soybean seeds. This technique aims to minimize the negative influence of biotic and abiotic factors on seed physiological quality at the end of the plant cycle. However, forced seed maturation with the application of herbicides can compromise the acquisition of seed quality components, especially longevity. Thus, the objective of this research was to evaluate the physiological quality of soybean seeds subjected to forced maturation with desiccants. The experiment was performed in a completely randomized design, with a treatment consisting of soybean plants subjected to the application of herbicides with desiccant action at stage R7.3 and another that underwent the natural process of maturation, that is, without herbicide application. The herbicide used was Paraquat. Seed germination, vigor (first germination count, dry mass, seedling length, time to reach 50% germination(t50), emergence index, and emergence speed), and longevity(P50) were evaluated. The herbicides did not affect germination (normal seedlings). However, the acquisition of vigor and longevity, and the preservation of seed vigor during storage were affected. Thus, the results indicate that the application of herbicide with desiccant action interrupts the process of acquisition of seed physiological quality, notably longevity in soybean seeds.

The Effect of Magneto-Priming on the Physiological Quality of Soybean Seeds

Microwaves have been applied to the drying of seeds of several species due to their maintenance of the quality of the seeds and reduction of time and costs. However, few is known about the effect of microwaves on the increase of the physiological quality of soybean seeds and especially their effects on longevity. Therefore, the use of microwaves as magneto-priming in soybean seeds was the object of study in this work. For this purpose, two soybean cultivars were selected and submitted to the ultra-high frequency (UHF) microwave exposure of 2.45 GHz, in the wavelength of 11 cm, and power of 0.2 W/g, for 15 min. The results showed that this condition of exposure to the microwave brought benefits in both cultivars after treatment. Incremental improvements were observed in the germinability indexes, the seedling length, the water absorption by the seeds, the fresh mass, dry mass, and longevity. The genes related to seed germination and longevity showed superior expression (HSFA3, HSP21, HSP17.6b, EXP, ABI3) with magneto-priming treatment. The data found ensure the use of the technique as a viable option for pre-treatment as magneto-priming in soybean seeds in order to improve seed quality.

Physiological Quality of Soybean Seeds Subjected to Industrial Treatment Before and After Storage

This research work aimed to evaluate the physiological quality of soybean seeds subjected to different chemical treatments, before and after seed treatment, throughout conventional storage. A completely randomized design was adopted, with 4 replications, in which the treatments were arranged in a 2 × 7 × 6 factorial scheme (time of treatment (before and after) × industrial seed treatment (IST) × storage period (0, 15, 30, 45, 60 and 90 days). For each IST, the specific volume of slurry was 0, 700, 900, 1400, 900, 1100 and 1600 100 kg-1 of seeds, respectively. A total of 2.5 kg of seeds, cultivar BMX Alvo RR, were used. After being treated, the seeds were placed in kraft paper bags and stored at controlled temperature and humidity in a cold chamber. Their physiological quality was evaluated after each storage period using standard germination test, first germination count, emergence speed index, final emergence in sand substrate, accelerated aging, radicle length, shoot length, and whole seedling length. Their physiological quality was reduced in treatments with higher volumes of slurry. Deleterious effects on vigor were observed with increasing storage period, both before and after IST. After seed treatment, the mean of the analyzed variables was considered higher, compared to the time prior to seed treatment.

Physiological Quality of Soybean Seeds as a Function of Soil Management Systems and Pre-Harvest Desiccation

Soil management systems directly interfere in the soil–plant relationship. However, there are still few studies evaluating the influence of long-term management systems on the physiological quality of soybean seeds. Another little-known topic is the influence of pre-harvest desiccation on the physiological quality of soybean seeds, especially on seed longevity. Thus, the aim of this research was to evaluate the physiological quality of soybean seeds cultivated under conventional tillage and no-tillage systems with and without desiccant use. The experiment was carried out in design is a split plot in a randomized complete block design. The treatments consisted of soil management systems (conventional tillage and no-tillage), with and without pre-harvest desiccation. In the treatment with desiccation, the herbicide Paraquat was applied, when the plants were at the R7.3 phenological stage (most of the seeds had a yellowish coat, with a shiny surface and were already detached from the pod). Seed germination, vigor (first germination count, seedling dry mass, seedling length, time to reach 50% germination (T50), seedling emergence and emergence speed index) and longevity (P50) were evaluated. Seeds cultivated under conventional tillage showed greater vigor for most traits evaluated, with values of T50 and seedling length higher by 24.39% and 24.77%, respectively, compared to NT. In addition, non-desiccation increased the seedling length and dry mass, in 15.45% and 21.59%, respectively. The use of desiccant aiming at seed vigor is dependent on the soil management system. Soybean seed longevity was superior in the no-tillage system, but desiccant application reduced seed longevity.

Recovery of Bradyrhizobium cells and effects on the physiological quality of soybean seeds sown in dry soil

Abstract: Farmers sometimes sow soybean (Glycine max (L.) Merrill) in dry soil in the expectation of rain in the short time. However, the forecast may not confirm, letting the inoculated seeds in the dry soil indefinitely. We assessed the survival of inoculated Bradyrhizobium and physiological quality of soybean seeds sown in dry soil. In the first experiment, irrigation was applied with 2 h, 1, 4, 11, 18, or 21 days after sowing; in the second experiment, sowing was carried out 2 h, 1, 5, 12, 14, or 20 days before irrigation. Each time represented a treatment in a completely randomized design. Bradyrhizobium cells dropped from ~8-9 × 104 colony forming units per seed soon after inoculation to -60% at 2 h after sowing in dry soil, and decreased to close to zero with time in both experiments. Although there was no effect on germination (59% and 81% in the first and second experiments, respectively), the exposure to dry soil reduced the emergence speed index from 19.5 (2 h) to 12.0 (21 days) in the first experiment and from 37.8 (2 h) to 13.8 (21 days) in the second. In the first experiment, the number of abnormal seedlings increased from 7% (2 h) to 24% (21 days); in the second, cotyledons showed cracks, which increased from 1% (2 h) to ~50% (≥ 5 days). Sowing in dry soil negatively affects not only the inoculated Bradyrhizobium, but also the physiological quality of soybean seeds.

Viability and vigor of soybean seeds as a result of industrial seed treatment and stored in different environments

The industrial seed treatment (IST) of soybeans is an economically recommended technique in agricultural production, providing protection against pests and initial pathogens. However, the real effects of pesticides about the quality of soybean seeds are still little known, since the adoption of the system is still recent by soybean farmers. The objective of this work was to evaluate the physiological quality of soybean seeds, treated or not, with insecticide/ fungicide new mixtures and stored for 240 days in a non-controlled and controlled environment. The completely randomized design was used, distributed in a 5 x 2 x 6 factorial scheme with four replications. The plot treatments consisted of soybean seeds belonging to the cultivar M - 7739 IPRO, treated with six different insecticide/ fungicide mixtures {(Cruiser®); (Amulet®); (MaximAdvanced®); (Cruiser®+ MaximAdvanced®); (MaximAdvanced® + Amulet®); (untreated control)}, stored in two environments {(laboratory – without control and cold room – with control (10 ± 2 ºC,45± 2% UR)} for eight months of storage with five evaluation times (0; 60; 120; 180 and 240days). The following tests were carried out: water content, germination, first count, seedling length and seedling dry mass. It was found that the chemical treatments negatively affect the germination potential of seeds, especially after 120 days of storage, regardless of the environment.Thiamethoxamand Thiamethoxam + Metalaxil; Tabendazole; Fludioxonil after storage provided the biggest reductions in seed viability and vigor after 240 days of storage. The cold room environment provides better conditions for the conservation of the germinative potential of soybean seeds.

EVALUASI MUTU FISIOLOGIS BENIH KEDELAI KUNING (Glycine max L.) PADA PENYIMPANAN TERBUKA

Quality soybean seeds are one of the essential factors to support soybean production. One of the obstacles in providing quality soybean seeds was the physiological quality of soybean seeds declined speedily during storage, especially in open storage. This study aimed to evaluate the physiological quality of yellow soybean seeds during a six-month storage period. This research was carried out from October 2020 to March 2021 in the seed analysis laboratory of the Seed Technology Study Program, Lampung State Polytechnic. The study used a Split-Plot Completely Randomized Design (CRD) with four replications. The main plot was the seed storage period which consists of seven levels, specifically 0, 1, 2, 3, 4, 5, and 6 months. Sub-plots were soybean variety which consists of two levels, specifically Dega variety and Deja variety. The results of the study indicated that (1) Deja variety had higher physiological quality than Dega variety based on germination, vigor index, growth speed, growth, maximum growth potential, and seed moisture content during the six-month storage period (2) Deja variety had longer storability than Dega variety in open storage.Keywords: Soybean, Physiological quality, Open storage.

Influence of spatial soil variability on productivity and physiological quality of soybean seeds

Seed production fields present spatial and temporal variability in the physical and chemical characteristics of the soil, thus influencing the yield and quality of the produced seeds. The objective of this study was to characterize the spatial variability in soil attributes providing physiological quality and productivity in soybean seeds in two commercial production fields, aiming to define areas of agronomic management. The experiment was carried out in the 2014/15 and 2015/16 crop seasons in a 100×100 m grid. Soil sampling was performed at a depth of 0-200 mm with a 75×75 m grid. The soybean samples were collected at the end of the 2014/15 crop season. Therefore, soil analysis was determined only in the 2014/15 crop season. The plants' agronomic characteristics and the productivity and physiological quality of produced seeds were determined for both crop seasons. Thus, it was concluded that the study site presented spatial variability in soil characteristics, phosphorus content, organic matter, cation exchange capacity, sand, and clay content, and the agronomic characteristics of soybean plants cv. BMX Desafio RR, including plant height, number of nodes per plant, number of pods with three seeds, number of pods with seeds, number of seeds per plant, seed weight per plant, seed weight per linear meter, productivity, and germination. Seed productivity showed a strong spatial dependence, while germination showed a moderate spatial dependence. Three management zones were determined according to the spatial distribution of the results obtained.

Herbicides in soybean development: productive aspects and physiological quality of seeds

The objective was to evaluate the production components of soybean and the physiological quality of seeds according to the application of subdoses of herbicides at the reproductive stage of healthy plants. The treatments consisted of the application of glyphosate (122.5 g acid equivalent [ae] ha-1), chlorimuron (1.75 g active ingredient [ai] ha-1), 2,4-D (134 g ae ha-1), fomesafen (60 g ai ha-1), glufosinate (55 g ai ha-1), paraquat (35 g ai ha-1), and control (no application). The chlorophyll index, agronomic performance, and physiological quality of seeds were evaluated. Glyphosate, chlorimuron, fomesafen, or glufosinate resulted in an increase in soybean plant height. Chlorimuron increased yield compared to the control. Paraquat or glufosinate, despite the injury, did not reduce yield, but the application of these herbicides is not recommended to stimulate plant development, given the potential for injury. The 2,4-D was the only one that caused a reduction in the physiological quality of soybean seeds.

MACHINE LEARNING FOR SOYBEAN SEEDS LOTS CLASSIFICATION

The seed germination and vigor evaluation are essential for the sowing sector to measure the performance of different seed lots and improve the efficiency of storage and sowing processes. However, the analysis of various tests to determine seed quality generates a large amount of information, making it almost impossible for humans to perform a quick and effective quality control analysis. Therefore, the objective of this study was to evaluate the differences in the physiological quality of soybean seeds in different cultivars using machine learning techniques to rank the lots based on their quality. Three cultivars were used, and the analysis was germination, accelerated aging, tetrazolium treatment, seedling emergence, and 1000 seed weight from 65 lots were measured. The lots were evaluated in two phases, one immediately after harvest and the other after six months of storage. Random forest, multi-layer perceptron, J48, and classification via regression classifiers were used, aided by the feature resampler technique. Random forest and classification via regression obtained the highest accuracy, and the random forest technique obtained the best results. Therefore, it is possible to classify soybean seed lots with great accuracy and precision using artificial intelligence and machine learning techniques.

Genetic control of soybean seed quality using partial diallel

Abstract: The selection of soybean genotypes with seeds of high physiological quality is key to increasing the likelihood of establishment and success in the field and thus reaping higher yields. The aim of this study was to evaluate the genetic control of the physiological quality of soybean seeds from a partial diallel cross. Six previously selected soybean cultivars, group 1 (CD 201, CA 115, MS 8400) and group 2 (CD 202, Syn 1263, Syn 1279), were intercrossed by a partial diallel cross, totalizing 24 treatments. Seeds from these cultivars and crosses were evaluated for seed physiological quality based on germination tests, first germination count, accelerated aging, seedlings emergence, and emergence speed index. The lignin content in the soybean seed coat was evaluated. The effects on general and specific combining ability and reciprocal effects were analyzed. There were significant additive and non-additive effects of the genes on the seed quality traits and pronounced effects on the reciprocal traits, which suggest the presence of a maternal effect. Lignin content was not correlated with the physiological test results in the studied genotypes.

Physiological quality of soybean seeds and the influence of maturity group

Abstract: Productive soybean genotypes is one of the main objectives of soybean breeding programs. However, there are few studies on the selection of characteristics associated with the quality and performance of soybean seeds. The objective in this study was to predict physiological and genetic parameters associated with seed quality in segregating soybean populations derived from parents of differing maturity groups. Eight genotypes of the F2:3 segregating soybean population (numbered 163, 24, 57, 108, 164, 169, 157, and 81) cultivated in the 2018/19 harvest were evaluated. To conduct the experiment, a completely randomized design was used. The physiological quality of the seeds was evaluated by germination test, tetrazolium test, and image analysis. Statistical analyses were performed by using a mixed model approach. Greater seed vigor was observed in the genotypes 108, 164, and 169, whose results were among the best for most of the analyzed parameters. The greater mechanical damage and damage due to stink bugs can explain the lower vigor of some seeds, as observed in genotypes 57 and 163, while weathering damage did not correspond to the results of the seed vigor tests. The analyses indicated a greater influence of genetic characteristics than of relative maturity group on the responses of the genotypes. No effect of relative maturity group on soybean seed vigor was evident.

Outcomes of water deficit stress on the physiological quality of soybean seeds

Abstract: This study aimed to compare the physiological seed quality of soybean cultivars produced under or without water deficit stress. Seed samples of three soybean cultivars produced in the 2019/2020 crop season were collected in a rural property and in a company that obtains the cultivars. In the rural property, the seeds were produced under water deficit stress and high temperatures during the crop’s and were characterized by the presence of greenish seeds. In the company that obtains the cultivars, the sample consisted of a certified seed lot produced under normal rainfall and temperature conditions, without greenish seeds. Germination, average germination time, germination speed index, average germination speed, the number of normal and abnormal seedlings, the seedling height and dry weight, the root evaluations (length, surface area, volume, average diameter and dry mass) and the total seedling dry mass were also evaluated. Soybean seeds produced under water deficit stress showed a reduction in physiological quality directly proportional to the percentage of greenish seeds present in each seed lot. The occurrence of water deficit stress on the soybean in the reproductive and maturation stages negatively impacts the soybean crop installation in the subsequent season due to the low physiological seed quality.

Precision agriculture: soybean culture in South Brazil

The objective of describing the scientific production on precision agriculture for soybean cultivation in southern Brazil. Integrative review developed in five stages: 1) to identify research problems: what is the scientific production on precision agriculture for the soybean economy in Brazil, 2) data collection, 3) data evaluation, 4) analysis and interpretation of data and 5) presentation of results. 19 articles appeared, published in the period from 2011 to 2019. All involve the study of soy, created in Latosol, Cambisolo and Argisolo; study areas ranging from 1.74 to 225 hectares; predominance of no-tillage carried out over ten years; crop rotation in summer and winter. As topics addressed: Physiological quality of soybean seeds; Sowing of soybeans; Chemical and physical attributes of the soil; Spectral indices during soybean growth; Weeds and pest control. There was greater emphasis on technologies: digital maps, machinery, tests, computer systems / programs, software, georeferencing, soil analysis, electronic meters, digital images, equipment and optical sensors. The tools used in precision agriculture are important for professional qualification, increased reduction, environmental impacts, sustainability, optimization of time and products and services, cost reduction.

Physiological quality of soybean seeds grown under different low altitude field environments and storage time

The use of high-quality seeds is essential to maintain high rates of production and productivity. The physiological quality of seeds obtained in the field is directly correlated to storage conditions and storage time. This study aimed to evaluate the physiological quality of soybean seeds in relation to different field environments (seed lots) and storage time. Commercial lots of seeds of the soybean cultivar M8349 IPRO were stored for three and six months. Seed moisture content was determined before and after accelerated aging, along with seed germination percentage and vigour evaluations performed before and after each storage period. The experiment was carried out as a completely randomised factorial design (10 × 3): with ten seed lots and three storage periods. The data were analysed by ANOVA, and the means of four independent replicates for each parameter evaluated were compared using the Scott-Knott test at 5% probability (P ≤ 0.05). Our results revealed that the low altitude regions where the seed samples were collected are suitable for soybean seed production with high physiological quality. Seed storage for six months does not cause a significant reduction in subsequent soybean seed field performance.