The recognition of high-speed demands in football has led elite academies to prioritize acceleration capabilities for player selection and promotion, particularly given their fundamental role in the motor skills of professional players and their impact on goal-related opportunities. This study explored the effectiveness of game-based versus resisted sprint training methods in enhancing the acceleration abilities of professional women’s football players. Over the entire competitive period, the training load of 26 athletes (24.2 ± 3.7 years) was assessed using GPS devices, and sprint capabilities were evaluated through four 30-m acceleration tests spaced six weeks apart. Linear mixed models (LMMs) analyzed physical load parameters, including distance covered at high speeds, speed events, and maximum speed, with periods and players as fixed and random effects, respectively. Significant sprint performance improvements were observed across all intervals, particularly when high-intensity distance volumes were combined with resisted sprint training. Conversely, high-intensity running without additional stimuli also led to performance gains, albeit to a lesser extent. Both game-based and resisted sprint training methods were effective in enhancing acceleration capabilities, while the absence of specific sprint focus did not significantly alter sprint performance. These findings support the inclusion of tailored sprint training in athletic programs to optimize acceleration in women’s football players.
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