Physical Interaction Research Articles

Exploring Elementary School Children’s Interaction at the School Threshold: Evidence From Athens, Greece

The school entrance is one of the most important places in the everyday lives of children. As an intersection between school and public realm of the city, it is characterized by gradations of porous and rigid boundaries. Depending on its function, it can serve as a threshold or as a boundary. Additionally, it is a spatial condition that facilitates a dialogue between the school and the city and draws content from both. School thresholds are important in supporting the role of the school as an important node in the city and a bridge between children’s various everyday life dynamics by demonstrating meaning as a place that is open to the community as well as to possibilities and physical interaction. In this research, we examine the role of the “realm of the in-between” between school and city through the architecture workshops for children at an Elementary School in the heart of Athens, in which we explore children’s perceptions, wishes, and ideas related to their familiar everyday places of transition from school to city and vice versa. The aim of the research is to find out: (a) What is the role of school entry today? (b) What is the children’s perception of the entrance of the school? (c) What are children’s wishes in relation to the spatial transition from city to school to better meet their needs and desires? (d) What are the specific qualities of a school entry that render it an ideal space for the interaction between children and with the space around them? and (e) How does the synergy of cinematic-architectural tools feed, enrich, and open up new possibilities in understanding and representing spatial and social phenomena?

Aspergillus terreus IFM 65899-THP-1 cells interaction triggers production of the natural product butyrolactone Ia, an immune suppressive compound.

We focused on the possibility that pathogenic microorganisms might produce immune suppressors to evade the action of immune cells. Based on this possibility, we have recently developed new co-culture method of pathogenic actinomyces and immune cells, however, the interaction mechanism between pathogens and cells was still unclear. In this report, co-culturing pathogenic fungi and immune cells were investigated. Pathogenic fungus Aspergillus terreus IFM 65899 and THP-1 cells were co-cultured and isolated a co-culture specific compound, butyrolactone Ia (1). 1 inhibits the production of nitric oxide by RAW264 cells and exhibits regulatory effects on autophagy, suggesting 1 plays a defensive role in the response of A. terreus IFM 65899 to immune cells. Furthermore, dialysis experiments and micrographs indicated that "physical interaction" between A. terreus IFM 65899 and THP-1 cells may be required for the production of 1. This is the first report of co-culture method of fungi with immune cells and its interaction mechanism.

The Development of Performing Folk Songs Model to Develop Holistic Health in Elderly

This research aims to study the problems and factors in organizing activities to promote holistic health in the elderly, Elderly Social Welfare Development Center, and to develop activities in the art of performing folk songs to promote holistic health in the elderly, Elderly Social Welfare Development Center. It is a study that aims to extract knowledge about the process of organizing activities for the elderly in order to develop a form of folk song performing arts activities to develop holistic health in the elderly as a research and development model Divided into 3 phases: Phase 1 synthesizes knowledge on organizing activities for the elderly, Phase 2 develops and evaluates traditional performing arts activities to develop holistic health in the elderly, and Phase 3 uses Folk performing arts activity format for holistic health development in the elderly. The research tool used was a structured interview form and satisfaction assessment form for organizing activities. All analysis results are presented as descriptive data and tables with explanations. The findings revealed Problems and factors in organizing activities to promote holistic health in the elderly at the Elderly Social Welfare Development Center. The researcher can summarize into two issues: 1) The current condition of organizing activities to promote the health of the elderly at the 12 Elderly Social Welfare Development Centers has three formats: ordinary elderly, particular types, and centers without elderly residents. In terms of operations for caring for and promoting the elderly, there will be an operation plan for the center that promotes the health of the elderly. There are four areas: physical, mental, and social interaction and intelligence. 2) Factors in organizing activities to promote holistic health in the elderly. The Center for the Development of Social Welfare for the Elderly found that opinions and needs for bringing in local wisdom heritage, in terms of performing arts, all four regions came to organize activities to promote the health of the elderly at all 12 Elderly Social Welfare Development Centers. There were two issues: 1) Perspectives and opinions. It was found that bringing both local performing arts and four regionally organized activities to promote the health of the elderly within the center. It is a good and exciting activity. Most elderly people enjoy fun activities, including singing and dancing. It is also a matter of Folk performing arts that can understand the emotions and feelings of the elderly very well. It also helps promote and stimulate the memory of the elderly. 2) The need to bring local wisdom and heritage. In terms of performing arts, all four regions came to organize activities to promote the health of the elderly at all 12 Elderly Social Welfare Development Centers. It was found that there was a need to organize activities to promote the health of the elderly using all Folk performing arts. The aim is to organize activities that can develop the physical, mental, social, and intellectual aspects. The results of the development of activities in the art of performing folk songs to promote holistic health in the elderly have two crucial points: 1) The uniqueness of the art of folk song performance that has an impact on society is that it reflects the culture of the community, build relationships among people in the community, have fun public relations media and self-development 2) The results of creating and developing an art activity format for performing folk songs to promote holistic health in the elderly. The researcher created and developed a folk song performance art activity model consisting of 6 elements: principles of the form, Objectives of the format, content, activities, promoting factors, and evaluation

In vivo functional significance of direct physical interaction between period and cryptochrome in mammalian circadian rhythm generation

Abstract In the current model, the auto-negative feedback action of Period (Per) and Cryptochrome (Cry) on their own transcription is the hallmark mechanism driving cell-autonomous circadian rhythms. Although this model likely makes sense even if Per and Cry undertake this action in a mutually independent manner, many studies have suggested the functional significance of direct physical interaction between Per and Cry. However, even though the interaction is a biochemical process that pertains to the fundamentals of the circadian oscillator, its in vivo contribution to circadian rhythm generation remains undefined. To answer this question, we focused on zinc coordination between Per and Cry, whose contribution to circadian rhythm generation remains undefined. Specifically, we aimed to impair endogenous Per-Cry association by introducing an amino acid substitution to zinc-coordinating residues located at the Per1 and Per2 C-terminal facing Cry in mice. These mice did not show severe impairment in the Per-Cry physical interaction, but rather a shortened period and decreased robustness in circadian rhythms at the tissue-autonomous and whole-body levels. Furthermore, these mice also showed a decreased in Per half-life, suggesting that impaired fine-tuning of Per half-life caused abnormal circadian period and robustness in vivo. We also found a minor but significant impact of a reindeer-specific Per2 mutation located in the Per-Cry interface on circadian rhythms in vivo. These lines of evidence indicate that only partial impairment of the Per-Cry physical interaction produces a substantial effect on circadian period and robustness, supporting the in vivo functional significance of the interaction.

Nanotechnology in healthcare, and its safety and environmental risks.

Nanotechnology holds immense promise in revolutionising healthcare, offering unprecedented opportunities in diagnostics, drug delivery, cancer therapy, and combating infectious diseases. This review explores the multifaceted landscape of nanotechnology in healthcare while addressing the critical aspects of safety and environmental risks associated with its widespread application. Beginning with an introduction to the integration of nanotechnology in healthcare, we first delved into its categorisation and various materials employed, setting the stage for a comprehensive understanding of its potential. We then proceeded to elucidate the diverse healthcare applications of nanotechnology, spanning medical diagnostics, tissue engineering, targeted drug delivery, gene delivery, cancer therapy, and the development of antimicrobial agents. The discussion extended to the current situation surrounding the clinical translation and commercialisation of these cutting-edge technologies, focusing on the nanotechnology-based healthcare products that have been approved globally to date. We also discussed the safety considerations of nanomaterials, both in terms of human health and environmental impact. We presented the in vivo health risks associated with nanomaterial exposure, in relation with transport mechanisms, oxidative stress, and physical interactions. Moreover, we highlighted the environmental risks, acknowledging the potential implications on ecosystems and biodiversity. Lastly, we strived to offer insights into the current regulatory landscape governing nanotechnology in healthcare across different regions globally. By synthesising these diverse perspectives, we underscore the imperative of balancing innovation with safety and environmental stewardship, while charting a path forward for the responsible integration of nanotechnology in healthcare.

Decoding food losses in pulses trade: proposing sustainable solutions from an Indian perspective

ABSTRACT Food losses are a critical concern for economic, social, and environmental sustainability within logistics systems. This paper quantifies food losses in India's pulses trade, examining specific cases and identifying causes and stages of losses in the international food supply chain. Focusing on sustainable development goals, it offers insights into food losses in internationally traded cargo (pulses), which rank second in the FAO-Food Loss Index and are India's second-largest imported food product. Using a descriptive research methodology, the study unravels the complexities of food losses in India's pulse supply chain. Primary data were collected via questionnaires from 130 importing firms and supply chain professionals, supplemented by secondary sources. Purposive sampling guided data collection from April 2023 to May 2024, involving virtual and physical stakeholder interactions. The comprehensive analysis provides strategies to mitigate food losses, laying the groundwork for improvements in India and other developing countries.

Explicit description of viral capsid subunit shapes by unfolding dihedrons.

Viral capsid assembly and the design of capsid-based nanocontainers critically depend on understanding the shapes and interfaces of constituent protein subunits. However, a comprehensive framework for characterizing these features is still lacking. Here, we introduce a novel approach based on spherical tiling theory that explicitly describes the 2D shapes and interfaces of subunits in icosahedral capsids. Our method unfolds spherical dihedrons defined by icosahedral symmetry axes, enabling systematic characterization of all possible subunit geometries. Applying this framework to real T = 1 capsid structures reveals distinct interface groups within this single classification, with variations in interaction patterns around 3-fold and 5-fold symmetry axes. We validate our classification through molecular docking simulations, demonstrating its consistency with physical subunit interactions. This analysis suggests different assembly pathways for capsid nucleation. Our general framework is applicable to other triangular numbers, paving the way for broader studies in structural virology and nanomaterial design.

Fall Detection in Q-eBall: Enhancing Gameplay Through Sensor-Based Solutions

The field of physically interactive electronic games is rapidly evolving, driven by the fact that it combines the benefits of physical activities and the attractiveness of electronic games, as well as advancements in sensor technologies. In this paper, a new game was introduced, which is a special version of Bubble Soccer, which we named Q-eBall. It creates a dynamic and engaging experience by combining simulation and physical interactions. Q-eBall is equipped with a fall detection system, which uses an embedded electronic circuit integrated with an accelerometer, a gyroscopic, and a pressure sensor. An evaluation of the performance of the fall detection system in Q-eBall is presented, exploring its technical details and showing its performance. The system captures the data of players’ movement in real-time and transmits it to the game controller, which can accurately identify when a player falls. The automated fall detection process enables the game to take the required actions, such as transferring possession of the visual ball or applying fouls, without the need for manual intervention. Offline experiments were conducted to assess the performance of four machine learning models, which were K-Nearest Neighbors (KNNs), Support Vector Machine (SVM), Random Forest (RF), and Long Short-Term Memory (LSTM), for falls detection. The results showed that the inclusion of pressure sensor data significantly improved the performance of all models, with the SVM and LSTM models reaching 100% on all metrics (accuracy, precision, recall, and F1-score). To validate the offline results, a real-time experiment was performed using the pre-trained SVM model, which successfully recorded all 150 falls without any false positives or false negatives. These findings prove the reliability and effectiveness of the Q-eBall fall detection system in real time.

Sustainable digital rent: a transformative framework for value dynamics in the digital age

IntroductionThis paper introduces the concept of Sustainable Digital Rent (SDR), highlighting the shift from traditional economic rent based on tangible assets to rent derived from digital platforms. At the heart of this shift is the “value state,” a dynamic balance between constructive expectations and destructive information. As digital platforms generate increasing amounts of information, expectations are increasingly met and shared more efficiently with all users, leading to a reduction in individual and general motivational, emotional, and cognitive engagement. These platforms, now essential to modern life, facilitate online activities that reduce as well physical engagement and natural interactions, thereby impacting cognitive function and physical health. By extracting rent directly, digital platform operators limit the benefits users could gain to support their mental and physical well-being.MethodsThis paper empirically defines and estimates SDR using the collective estimates of price, cost, and income (PCI) as practiced in North American real estate appraisal, demonstrated through abstract art rent. Our approach provides a new perspective on valuing intangible assets, such as knowledge, by showing the shift from expectation to information, governed by the value state in cognitive evaluations. Emphasizing interdisciplinary relevance, the method underscores the need for an efficient mechanism to redistribute SDR benefits to digital platform users, supporting fair and equitable digital development.Results and discussionThe results show that digital rent is driven primarily by cognitive and informational content, demonstrating the need for redistribution mechanisms to address the growing inequality on digital platforms. The use of abstract art as a case study provides a convenient and illustrative way to explore how intangible assets, like digital rents, can be evaluated and redistributed. SDR offers insights into how digital rents can be captured and redistributed equitably, ensuring that platform users and creators benefit from the knowledge economy’s growth. The findings underscore the relevance of measuring SDR to guide policy recommendations aimed at reducing digital monopolization and promoting sustainable digital development.

First Nations Peoples' perspectives on telehealth physiotherapy: a qualitative study focused on the therapeutic relationship.

Relationships are the core of Indigenous Peoples' spiritual and cultural identities, and therapeutic relationships are an integral part of the physical rehabilitation process, directly influencing health outcomes. However, participating in therapeutic relationships can be difficult for First Nations Peoples, particularly in the virtual landscape. There is limited understanding of First Nations Peoples' perspectives on this issue, and this understanding is crucial to developing culturally safe and effective telehealth physiotherapy programs. Therefore, the purpose of this study is to explore the perspectives of First Nations Peoples from British Columbia, Canada, on telehealth physiotherapy, with an emphasis on the virtual therapeutic relationship. A narrative qualitative study that utilized one-on-one, semistructured interviews was conducted with 19 First Nations adults from remote and rural First Nations communities in north-central British Columbia, Canada. Interviews were recorded, transcribed verbatim, and analyzed using an inductive approach to reflexive thematic analysis. Three themes emerged from data analysis. 'Therapist's attitude and rapport' captures participants' perceptions of what matters the most in how physiotherapists relate to First Nations Peoples and carry out their work. 'Remote nature of virtual care' encompasses the main challenges of virtual care visits, particularly how these were perceived to impact establishing and maintaining solid therapeutic relationships. 'Fostering culturally appropriate and safe telehealth physiotherapy' focuses on what is needed to advance telehealth physiotherapy in a manner that respects and reflects First Nations cultures, equipping all involved parties to provide comprehensive and sensitive services. Our findings advocate a hybrid model that combines in-person and telehealth visits to address communication barriers and the absence of physical interaction. Bridging the digital health literacy gap through training and collaboration with local support staff is crucial (as it is to bridge the possible cultural literary gap of therapists), and the incorporation of cultural elements holds promise for enhancing the engagement and effectiveness of telehealth services in these communities. The pursuit of equitable health care for First Nations communities demands not only increased access but also a thoughtful, culturally safe, trauma-informed, and holistic approach. This approach must be tailored to the unique needs of First Nations Peoples, emphasizing the integration of cultural elements and community support. A hybrid model combining in-person and telehealth visits is recommended to address logistical challenges and enhance the therapeutic relationship, ensuring that care is both effective and respectful of cultural values and practices.

Abstract 4134792: SIRTUIN5 Modulates Na + /Ca 2+ Handling Via Oxidative Stress Dependent Manner In Mouse Heart

Background: The cardiac Na + channel Na V 1.5 (encoded by SCN5A ) governs cardiac inward Na + current (I Na ) and the fast upstroke and plateau phases of the cardiac action potential. Mutations in Na V 1.5 can cause acquired or inherited arrhythmias and conduction diseases, including ~20% of cases of Brugada Syndrome (BrS). Changes in I Na can impact Ca 2+ handling and cardiac excitation-contraction coupling. We have previously shown that SIRT1-mediated deacetylation of Na V 1.5 increased I Na . Recently, potential mutations (including P114T) in SIRT5, another NAD + -dependent deACYLase in the Sirtuin family localized to mitochondria, were identified in small families with BrS. Hypothesis: Sirt5 dysfunction evokes arrhythmias via Na + and Ca 2+ mishandling in an oxidative stress-dependent manner in mouse hearts. Aims: To explore the potential role of SIRT5 in BrS using heterologous expression systems and homozygous P114T-Sirt5 knock-in (P114T-KI) mice. Methods: Protein expression and physical interactions were detected by immunoprecipitation and immunoblot. The effects of SIRT5 on Na + current was measured using patch clamp in HEK cells and mouse cardiac myocytes. Confocal microscopy was used to measure reactive oxygen species (ROS) and for Ca 2+ imaging. Results: Both WT and P114T-SIRT5 co-immunoprecipitate with Na V 1.5, but WT increased peak I Na in HEK cells while P114T did not (Fig A,B). Live-cell staining using DCFDA or mitoSOX showed that P114T-KI hearts had increased basal ROS and were more sensitive to oxidative stress induced by H 2 O 2 than WT littermates. P114T-KI hearts had increased Na + /Ca 2+ exchange protein 1 (NCX1) expression, and Langendorff-perfused hearts displayed abnormal Ca 2+ handling and arrhythmias (Fig C). Notably, treatment with the mitochondrial ROS scavenger mitotempo mitigated the aberrant Ca 2+ handling and arrhythmias. Conclusion: These findings suggest that the P114T-SIRT5 causes abnormal Na + and Ca 2+ handling and arrhythmias in a ROS-dependent manner, highlighting potential mechanisms underlying BrS. This finding may pave the way for the use of SIRT5 or its activators as novel anti-arrhythmic therapies in the future.

What It Means to Be Human: A Response to Harzheim.

This response engages critically with Harzheim's review of Thomas Fuchs' In Defense of the Human Being: Foundational Questions of an Embodied Anthropology. Fuchs' work offers a profound exploration of embodied cognition, arguing that human cognition and existence are deeply shaped by our physical interactions. Harzheim's critique highlights significant aspects of Fuchs' framework, including his critique of functionalist models, the impact of transhumanist technologies, and ethical concerns in healthcare technology. This paper extends Harzheim's review by proposing an integration of functionalist and embodied cognitive models, emphasizing the need for a comprehensive evaluation of technological impacts, and advocating for a more robust ethical framework that considers social equity. Additionally, it addresses the is-ought distinction and explores the implications of technological advancements on human identity and mental health. Doede's critique is also discussed, underscoring the importance of integrating diverse cognitive models and addressing technological determinism. Overall, this response calls for a more nuanced and inclusive approach to the discourse on embodied cognition, aiming to enrich the scholarly conversation and address the complexities and implications of Fuchs' analysis.

Extremely Ultrahigh Stretchable Starch‐Based Hydrogels with Continuous Hydrogen Bonding

AbstractNatural polysaccharides‐based hydrogels have drawn extensive attention yet have been plagued by less desirable stretchability due to their inherent nature. Here, ultra‐stretchable starch‐based hydrogels (amylopectin/polyacrylamide, AAM) are developed by constructing reversible intramolecular physical interactions. This strategy endows the hydrogel with exceedingly ultrahigh deformation due to a continuous hydrogen bonding network. It can be stretched from less than 0.5 to >300 cm without breakage that the elongation exceeds 600 times the original length. The elongation collected by the universal testing machine reaches up to 36 000% without breakage outperforming previous reports and demonstrating extraordinary stretchability. Furthermore, an interwoven structure of hydrogen bonding interaction and trace covalent bonds make the stress of hydrogel reach 0.28 MPa, accompanied by an ultra‐high strain of 22 500% and significant toughness (47 MJ·m−3). The hydrogel displays high transparency (≈93%), low‐temperature resistance, moisturizing property, and extraordinary interfacial adhesion property. Intriguingly, the aqueous precursor can act as inks to prepare various forms of hydrogel within minutes through the facile writing or drawing method. This hydrogel verifies strong potential in both fields of human motion sensor (After long‐term or low‐temperature conditions) and energy storage. This study will facilitate the progress of ultra‐stretchable or multifunctional hydrogels.

Design and simulation of a smart master switch system based on multi-input XOR logic gate

AbstractMechanical switches have been the conventional way of controlling electrical energy in different electrical systems such as: lighting systems, socket systems, and circuit breakers, especially in domestic, hospital, and industrial applications. Mechanical switches often require physical access to control different electrical devices that are connected to the power sources. The work presented in this paper aimed at designing and simulating a multi-input based Exclusive-Or master switch system that remotely controls the lighting and socket systems using the wireless switching mechanisms of Global System for Mobile communication and Bluetooth. The system therefore limits physical interaction that require use of the single pole double throw and keypad mechanical switches that are however included to act as fall-back mechanism in control of electrical devices. Within the recommended electrical safety measures, the design can be streamlined and integrated to remotely control lighting systems and sockets alongside the conventional mechanical switches in the consumer control unit.

Enhancing Human-Computer Interaction in Augmented Reality (AR) and Virtual Reality (VR) Environments: The Role of Adaptive Interfaces and Haptic Feedback Systems

Human-Computer Interaction (HCI) in Augmented Reality (AR) and Virtual Reality (VR) environments has emerged as a critical area of research, shaping how users interact with immersive technologies. This study explores the design and development of advanced HCI frameworks in AR/VR environments, with a particular focus on adaptive interfaces and haptic feedback systems. Adaptive interfaces employ real-time data to personalize user experiences, while haptic feedback systems provide tactile sensations to bridge the gap between digital and physical interactions. The integration of these technologies enhances user engagement, immersion, and efficiency across applications such as gaming, training simulations, healthcare, and remote collaboration. This research investigates the challenges in creating intuitive, responsive systems and examines cutting-edge solutions in multimodal interactions, gesture recognition, and real-time feedback processing. By analyzing user performance and satisfaction, this study contributes to the refinement of AR/VR systems, paving the way for next-generation immersive environments.

Examining students’ learning experiences in hybrid environments

In the aftermath of the pandemic, a generation of students who are comfortable with learning online and prefer having the autonomy to decide how they want to learn – in-person or remotely – has emerged. Hybrid learning, where teaching instruction is given to students in different spatial settings at the same time, is a modality that has the potential to deliver stimulating learning experiences to support diverse learning needs. The experiences in various forms of learning – in-person, fully online and hybrid – have exposed students to various modalities of learning and access to resources. However, the absence of physical presence and the lack of feedback from remote students has caused lecturers to be uncertain of the effectiveness of hybrid lectures and tutorials. Thus, understanding students’ learning and their considerations when determining their preferred modality will provide lecturers with clear strategies for supporting students. This qualitative study aims to examine students' learning experiences in a research-intensive public University in New Zealand university. The study was guided by the following questions: (i) What are students’ perceptions of learning in a hybrid environment?; and (ii) How do students learn in hybrid environments? In-depth interviews with 15 students who have experienced hybrid education in a research-intensive public university in New Zealand was conducted using a descriptive case study. Three categories that described students’ considerations and dispositions towards learning in hybrid environments, and how various conditions and resources within their learning environment influenced their behaviours emerged. Preliminary findings found that students’ dispositions towards learning were critical factors that contribute towards their behaviours in a hybrid environment. Unlike traditional conceptions of teaching and learning, hybrid education requires students to be active participants in their learning and take charge of their own learning in aspects such as deciding how they best learn. Despite minimal social and physical interaction with lecturers and their peers, students found hybrid learning to be effective. Interviews with students who had experienced hybrid education highlighted perspectives that differed from previous studies. Remote students showed willing and drive to excel in their studies despite being disconnected from their peers in the physical spaces, and did not feel that they had less of a learning experience. This finding echoes that of Zhong et al., where social presence was not found to be significantly related to learning persistence. Students found having immediate access to information and lecturers to be more important than whether they could communicate and interact with other in-person classmates. The study recommends strategies lecturers can employ to improve teaching practices in hybrid environments. This includes ensuring all materials are digitally captured synchronously and having a student partner to monitor questions or teaching assistant to address questions the chatroom will help remote students greatly. Further analysis of the current data will take a closer look at how students’ dispositions play a part in students’ engagement levels and their behaviours in hybrid environments.

A comprehensive two-hybrid analysis to explore the Legionella pneumophila effector-effector interactome.

Secreted bacterial effector proteins are typically viewed as modulators of host activity, entering the host cytosol to physically interact with and modify the activity of one or more host proteins in support of infection. A growing body of evidence suggests that a subset of effectors primarily function to modify the activities of other effectors inside the host. These "effectors of effectors" or metaeffectors are often identified through experimental serendipity during the study of canonical effector function against the host. We previously performed the first global effector-wide genetic interaction screen for metaeffectors within the arsenal of Legionella pneumophila, an intracellular bacterial pathogen with over 300 effectors. Here, using a high-throughput, scalable methodology, we present the first global interaction network of physical interactions between L. pneumophila effectors. This data set serves as a complementary resource to identify and understand both the scope and nature of non-canonical effector activity within this important human pathogen.

Early movement restriction impairs the development of sensorimotor integration, motor skills and memory in rats: Towards a preclinical model of developmental coordination disorder?

Children with neurodevelopmental disorders, such as developmental coordination disorder (DCD), exhibit gross to fine sensorimotor impairments, reduced physical activity and interactions with the environment and people. This disorder co-exists with cognitive deficits, executive dysfunctions and learning impairments. Previously, we demonstrated in rats that limited amounts and atypical patterns of movements and somatosensory feedback during early movement restriction manifested in adulthood as degraded postural and locomotor abilities, and musculoskeletal histopathology, including muscle atrophy, hyperexcitability within sensorimotor circuitry and maladaptive cortical plasticity, leading to functional disorganization of the primary somatosensory and motor cortices in the absence of cortical histopathology. In this study, we asked how this developmental sensorimotor restriction (SMR) started to impact the integration of multisensory information and the emergence of sensorimotor reflexes in rats. We also questioned the enduring impact of SMR on motor activities, pain and memory. SMR led to deficits in the emergence of swimming and sensorimotor reflexes, the development of pain and altered locomotor patterns and posture with toe-walking, adult motor performance and night spontaneous activity. In addition, SMR induced exploratory hyperactivity, short-term impairments in object-recognition tasks and long-term deficits in object-location tasks. SMR rats displayed minor alterations in histological features of the hippocampus, entorhinal, perirhinal and postrhinal cortices yet no obvious changes in the prefrontal cortex. Taken all together, these results show similarities with the symptoms observed in children with DCD, although further exploration seems required to postulate whether developmental SMR corresponds to a rat model of DCD.

Disruption of the Physical Interaction Between Carbonic Anhydrase IX and the Monocarboxylate Transporter 4 Impacts Lactate Transport in Breast Cancer Cells

It has been previously established that breast cancer cells exhibit high expression of the monocarboxylate (lactate) transporters (MCT1 and/or MCT4) and carbonic anhydrase IX (CAIX) and form a functional metabolon for proton-coupled lactate export, thereby stabilizing intracellular pH. CD147 is the MCT accessory protein that facilitates the creation of the MCT/CAIX complex. This study describes how the small molecule Beta-Galactose 2C (BGal2C) blocks the physical and functional interaction between CAIX and either MCT1 or MCT4 in Xenopus oocytes, which reduces the rate of proton and lactate flux with an IC50 of ~90 nM. This value is similar to the Ki for inhibition of CAIX activity. Furthermore, it is shown that BGal2C blocks hypoxia-induced lactate transport in MDA-MB-231 and MCF-7 breast cancer cells, both of which express CAIX. As in oocytes, BGal2C interferes with the physical interaction between CAIX and MCTs in both cell types. Finally, X-ray crystallographic studies highlight unique interactions between BGal2C and a CAIX-mimic that are not observed within the CAII active site and which may underlie the strong specificity of BGal2C for CAIX. These studies demonstrate the utility of a novel sulfonamide in interfering with elevated proton and lactate flux, a hallmark of many solid tumors.

The Arabidopsis blue-light photoreceptor CRY2 is active in darkness to inhibit root growth.

Cryptochromes (CRYs) are blue-light receptors that regulate diverse aspects of plant growth. However, whether and how non-photoexcited CRYs function in darkness or non-blue-light conditions is unknown. Here, we show that CRY2 affects the Arabidopsis transcriptome even in darkness, revealing a non-canonical function. CRY2 suppresses cell division in the root apical meristem to downregulate root elongation in darkness. Blue-light oligomerizes CRY2 to de-repress root elongation. CRY2 physically interacts with FORKED-LIKE 1 (FL1) and FL3, and these interactions are inhibited by blue light, with only monomeric but not dimeric CRY2 able to interact. FL1 and FL3 associate with the chromatin of cell division genes to facilitate their transcription. This pro-growth activity is inhibited by CRY2's physical interaction with FLs in darkness. Plants have evolved to perceive both blue-light and dark cues to coordinate activation and repression of competing developmental processes in above- and below-ground organs through economical and dichotomous use of ancient light receptors.