Phosphate Composite Scaffolds Research Articles

Polycaprolactone/β-Tricalcium Phosphate Composite Scaffolds with Advanced Pore Geometries Promote Human Mesenchymal Stromal Cells' Osteogenic Differentiation.

Critical-sized mandibular bone defects, arising from, for example, resections after tumor surgeries, are currently treated with autogenous bone grafts. This treatment is considered very invasive and is associated with limitations such as morbidity and graft resorption. Tissue engineering approaches propose to use 3D scaffolds that combine structural features, biomaterial properties, cells, and biomolecules to create biomimetic constructs. However, mimicking the complex anatomy and composition of the mandible poses a challenge in scaffold design. In our study, we evaluated the dual effect of complex pore geometry and material composition on the osteogenic potential of 3D printed scaffolds. The scaffolds were made of polycaprolactone (PCL) alone (TCP0), or with a high concentration of β-tricalcium phosphate (β-TCP) up to 40% w/w (TCP40), with two complex pore geometries, namely a star- (S) and a diamond-like (D) shape. Scanning electron microscopy and microcomputed tomography images confirmed high fidelity during the printing process. The D-scaffolds displayed higher compressive moduli than the corresponding S-scaffolds. TCP40 scaffolds in simulated body fluid showed deposition of minerals on the surface after 28 days. Subsequently, we assessed the differentiation of seeded bone marrow-derived human mesenchymal stromal cells (hMSCs) over 28 days. The early expression of RUNX2 in the cell nuclei confirmed the commitment toward an osteogenic phenotype. Moreover, alkaline phosphatase (ALP) activity and collagen deposition displayed an increasing trend in the D-scaffolds. Collagen type I was mainly present in the deposited extracellular matrix (ECM), confirming deposition of bone matrix. Finally, Alizarin Red staining showed successful mineralization on all the TCP40 samples, with higher values for the S-shaped scaffolds. Taken together, our study demonstrated that the complex pore architectures of scaffolds comprised TCP40 stimulated osteogenic differentiation and mineralization of hMSCs in vitro. Future research will aim to validate these findings in vivo.

Enhanced Bone Healing in Critical-Sized Rabbit Femoral Defects: Impact of Helical and Alternate Scaffold Architectures.

This study investigates the effect of scaffold architecture on bone regeneration, focusing on 3D-printed polylactic acid-bioceramic calcium phosphate (PLA-bioCaP) composite scaffolds in rabbit femoral condyle critical defects. We explored two distinct scaffold designs to assess their influence on bone healing and scaffold performance. Structures with alternate (0°/90°) and helical (0°/45°/90°/135°/180°) laydown patterns were manufactured with a 3D printer using a fused deposition modeling technique. The scaffolds were meticulously characterized for pore size, strut thickness, porosity, pore accessibility, and mechanical properties. The in vivo efficacy of these scaffolds was evaluated using a femoral condyle critical defect model in eight skeletally mature New Zealand White rabbits. Then, the results were analyzed micro-tomographically, histologically, and histomorphometrically. Our findings indicate that both scaffold architectures are biocompatible and support bone formation. The helical scaffolds, characterized by larger pore sizes and higher porosity, demonstrated significantly greater bone regeneration than the alternate structures. However, their lower mechanical strength presented limitations for use in load-bearing sites.

Graphene/β-Tricalcium Phosphate Composite Scaffold Facilitates the Repairment of Cartilage Defect in Rabbit Knee Joint

Our research aims to evaluate the repairment of graphene/β-tricalcium phosphate (G/β-TCP) composite scaffold on cartilage defect in rabbit knee joints. The G/β-TCP composite material containing BMSCs was surgically implanted into the femoral condyle of a full-thickness cartilage defect model in rabbits. XRD showed that no difference in the X-ray diffraction characteristics was observed between G/β-TCP and β-TCP. The biomechanical test claimed that the graphene doped β-TCP material processed higher mechanical strength. Scanning electron microscopy showed that the surface of G/β-TCP material was smoother and the texture was denser. The scaffold combined with BMSCs was transplanted into the full-thickness cartilage defect rabbit model and the results showed that the serum CRP level increased only 1 month after implantation, and the Cr level increased at 2 months after implantation, while G/β-TCP material showed rare significant pathological changes on the liver, spleen, kidney, brain, and soft tissue around the operation, which indicated a promising biocompatibility. The expression of type I and II collagen in the cartilage tissue of G/β-TCP treated rabbits was dramatically elevated compared to β-TCP at 2 and 3 months after implantation. Collectively, the G/β-TCP composite scaffold facilitated the repairment of cartilage defects in the rabbit knee joints.

Effect of Silicon Dioxide and Magnesium Oxide on the Printability, Degradability, Mechanical Strength and Bioactivity of 3D Printed Poly (Lactic Acid)-Tricalcium Phosphate Composite Scaffolds.

Poly (lactic acid) (PLA) is a biodegradable polyester that has been exploited for a variety of biomedical applications, including tissue engineering. The incorporation of β-tricalcium phosphate (TCP) into PLA has imparted bioactivity to the polymeric matrix. We have modified a 90%PLA-10%TCP composite with SiO2 and MgO (1, 5 and 10 wt%), separately, to further enhance the material bioactivity. Filaments were prepared by extrusion, and scaffolds were fabricated using 3D printing technology associated with fused filament fabrication. The PLA-TCP-SiO2 composites presented similar structural, thermal, and rheological properties to control PLA and PLA-TCP. In contrast, the PLA-TCP-MgO composites displayed absence of crystallinity, lower polymeric molecular weight, accelerated degradation ratio, and decreased viscosity within the 3D printing shear rate range. SiO2 and MgO particles were homogeneously dispersed within the PLA and their incorporation increased the roughness and protein adsorption of the scaffold, compared to a PLA-TCP scaffold. This favorable surface modification promoted cell proliferation, suggesting that SiO2 and MgO may have potential for enhancing the bio-integration of scaffolds in tissue engineering applications. However, high loads of MgO accelerated the polymeric degradation, leading to an acid environment that imparted the composite biocompatibility. The presence of SiO2 stimulated mesenchymal stem cells differentiation towards osteoblast; enhancing extracellular matrix mineralization, alkaline phosphatase (ALP) activity, and bone-related genes expression. The PLA-10%TCP-10%SiO2 composite presented the most promising results, especially for bone tissue regeneration, due to its intense osteogenic behavior. PLA-10%TCP-10%SiO2 could be used as an alternative implant for bone tissue engineering application.

Scaffold of Bone Tissue Engineering Based on PVA/BCP Bioactive Composite Foam

This study constructed poly (vinyl alcohol)/ biphasic-calcium phosphate (PVA/ BCP) composite scaffolds. The biphasic-calcium phosphate (BCP) was incorporated in 0, 5, 10, and 25 wt%; BP0, BP1, BP2, and BP3, respectively. The surface morphology was done with a scanning electron microscope (SEM) to observe the porosity and the pore size and distribution of fabricated samples. The Fourier Transform Infrared spectroscopy (FTIR), and some physical properties such as porosity, density, swelling ratio, flexural strength, impact strength, and compression strength were also investigated. The biodegradation and bioactivity were also tested. The SEM results showed that the pores increased and became more regular and interconnected to each other with the increasing addition of BCP. The density decreased with the addition of BCP, while the porosity and mechanical properties increased with additives. The sample of BP3 has a high porosity (67%) and high impact strength (11.9 MPa). The high porosity is favorable for bone implants, and the mechanical strength must also be considered. The bio tests show that the biodegradation became regular by adding the BCP powder, which leads to ease of controlling the gradual degradation and the samples are bioactive for bone tissue. Keywords: Bone Tissue Engineering, PVA, Biphasic-Calcium Phosphate, Porosity, Mechanical properties

Fabrication of 3D printed Ca3Mg3(PO4)4-based bioceramic scaffolds with tailorable high mechanical strength and osteostimulation effect

Calcium, magnesium and phosphate are predominant constituents in the human bone. In this study, magnesium-calcium phosphate composite bioceramic scaffolds were fabricated utilizing Mg3(PO4)2 and β-Ca3(PO4)2 as starting materials, and their pore structure was constructed by 3D printing. The porosity and compressive strength of the composite bioceramic scaffolds could be adjusted by altering the sintering temperature and the formula of starting materials. The composite bioceramic scaffolds prepared from 60 wt% Mg3(PO4)2 and 40 wt% β-Ca3(PO4)2 were dominated by the Ca3Mg3(PO4)4 phase, and this Ca3Mg3(PO4)4-based bioceramic scaffolds possessed the highest compressive strength (12.7 – 92.4 MPa). Moreover, the Ca3Mg3(PO4)4-based bioceramic scaffolds stimulated cellular growth and osteoblastic differentiation of bone marrow stromal cells. The Ca3Mg3(PO4)4-based bioceramic scaffolds as bone regenerative biomaterials are flexible to the requirement of bone defects at various sites.

Early In Vivo Osteogenic and Inflammatory Response of 3D Printed Polycaprolactone/Carbon Nanotube/Hydroxyapatite/Tricalcium Phosphate Composite Scaffolds.

The development of advanced biomaterials and manufacturing processes to fabricate biologically and mechanically appropriate scaffolds for bone tissue is a significant challenge. Polycaprolactone (PCL) is a biocompatible and degradable polymer used in bone tissue engineering, but it lacks biofunctionalization. Bioceramics, such as hydroxyapatite (HA) and β tricalcium phosphate (β-TCP), which are similar chemically to native bone, can facilitate both osteointegration and osteoinduction whilst improving the biomechanics of a scaffold. Carbon nanotubes (CNTs) display exceptional electrical conductivity and mechanical properties. A major limitation is the understanding of how PCL-based scaffolds containing HA, TCP, and CNTs behave in vivo in a bone regeneration model. The objective of this study was to evaluate the use of three-dimensional (3D) printed PCL-based composite scaffolds containing CNTs, HA, and β-TCP during the initial osteogenic and inflammatory response phase in a critical bone defect rat model. Gene expression related to early osteogenesis, the inflammatory phase, and tissue formation was evaluated using quantitative real-time PCR (RT-qPCR). Tissue formation and mineralization were assessed by histomorphometry. The CNT+HA/TCP group presented higher expression of osteogenic genes after seven days. The CNT+HA and CNT+TCP groups stimulated higher gene expression for tissue formation and mineralization, and pro- and anti-inflammatory genes after 14 and 30 days. Moreover, the CNT+TCP and CNT+HA/TCP groups showed higher gene expressions related to M1 macrophages. The association of CNTs with ceramics at 10wt% (CNT+HA/TCP) showed lower expressions of inflammatory genes and higher osteogenic, presenting a positive impact and balanced cell signaling for early bone formation. The association of CNTs with both ceramics promoted a minor inflammatory response and faster bone tissue formation.

Sintering densification mechanism and mechanical properties of the 3D-printed high-melting-point-difference magnesium oxide/calcium phosphate composite bio-ceramic scaffold

Over the past few years, biodegradable ceramic scaffolds have gained significant attention in the field of bone repair. Calcium phosphate (Ca3(PO4)2)- and magnesium oxide (MgO)-based ceramics are biocompatible, osteogenic, and biodegradable, making them attractive for potential applications. However, the mechanical properties of Ca3(PO4)2 are limited. We developed a magnesium oxide/calcium phosphate composite bio-ceramic scaffold characterized by a high melting point difference, using vat photopolymerization (VP) technology to address this issue. The primary goal was to fabricate high-strength ceramic scaffolds using biodegradable materials. In this study, we investigated ceramic scaffolds with varying MgO contents and sintering temperatures. We also discussed the co-sintering densification mechanism of high and low melting-point materials associated with composite ceramic scaffolds. During sintering, a liquid phase was generated, which filled up the pores generated during the vaporization of additives (such as resin) under the influence of capillary force. This led to an increase in the extent of ceramic densification realized. Moreover, we found ceramic scaffolds with 80 wt% MgO exhibited the best mechanical performance. This kind of composite scaffold performed better than pure MgO scaffold. The results reported herein highlight that high-density composite ceramic scaffolds can be potentially used in the field of bone repair.

3D-printed MgO nanoparticle loaded polycaprolactone β-tricalcium phosphate composite scaffold for bone tissue engineering applications: In-vitro and in-vivo evaluation.

Magnesium (Mg) plays an important role in controlling bone apatite structure and density and is a potential bioactive material in repairing critical-sized bone defects. In this study, we aimed to evaluate the effect of adding NanoMgO to polycaprolactone/beta-tricalcium phosphate (PCL/β-TCP) scaffolds on bone regeneration. Novel 3D-printed porous PCL/β-TCP composite scaffolds containing 10% nanoMgO were fabricated by fused deposition modeling (FDM) and compared with PCL/β-TCP (1:1) scaffolds (control). The morphology and physicochemical properties of the scaffolds were characterized by ATR-FTIR, XRD, scanning electron microscope-energy dispersive X-ray analysis (SEM-EDX), transmission-electron-microscopy (TEM), water contact angle, and compressive strength tests and correlated to its cytocompatibility and osteogenic capacity in-vitro. To evaluate in-vivo osteogenic capacity, bone-marrow-derived stem cell (BMSC)-loaded scaffolds were implanted into 8 mm rat critical-sized calvarial defects for 12 weeks. The hydrophilic scaffolds showed 50% porosity (pore size=504 μm). MgO nanoparticles (91.5± 27.6nm) were homogenously dispersed and did not adversely affect BMSCs' viability and differentiation. Magnesium significantly increased elastic modulus, pH, and degradation. New bone formation (NBF) in Micro-CT was 30.16 ± 0.31% and 23.56 ± 1.76% in PCL/β-TCP/nanoMgO scaffolds with and without BMSCs respectively, and 19.38 ± 2.15% and 15.75 ± 2.24% in PCL/β-TCP scaffolds with and without BMSCs respectively. Angiogenesis was least remarkable in PCL/β-TCP compared with other groups (p< .05). Our results suggest that the PCL/β-TCP/nanoMgO scaffold is a more suitable bone substitute compared to PCL/β-TCP in critical-sized calvarial defects.

Engineered 3D-Printed Polyvinyl Alcohol Scaffolds Incorporating β-Tricalcium Phosphate and Icariin Induce Bone Regeneration in Rat Skull Defect Model.

The skull defects are challenging to self-heal, and autologous bone graft repair has numerous drawbacks. The scaffolds for the rapid and effective repair of skull defects have become an important research topic. In this study, polyvinyl alcohol (PVA)/β-tricalcium phosphate(β-TCP) composite scaffolds containing icariin (ICA) were prepared through direct-ink three-dimensional (3D) printing technology. β-TCP in the composite scaffold had osteoconductive capability, and the ICA molecule had osteoinductive capacity. The β-TCP and ICA components in the composite scaffold can enhance the capability to repair skull defects. We show that ICA exhibited a slow-release behaviour within 80 days. This behaviour helped the scaffold to continuously stimulate the formation of new bone. The results of in vitro cell compatibility experiments showed that the addition of ICA molecules contributed to the adhesion and proliferation of MC-3T3-E1 cells. The level of alkaline phosphatase secretion demonstrated that the slow release of ICA can promote the osteogenic differentiation of MC-3T3-E1 cells. The introduction of ICA molecules accelerated the in situ bone regeneration in in vivo. It is concluded that the 3D-printed PVA scaffold with β-TCP and ICA has a wide range of potential applications in the field of skull defect treatment.

Coating of 3D printed PCL/TCP scaffolds using homogenized-fibrillated collagen

BackgroundPoly(3-caprolactone) (PCL)/β-tricalcium phosphate (β-TCP) composite scaffolds fabricated by three-dimensional (3D) printing are one of the common scaffolds for bone tissue regeneration. However, the main challenge of these 3D printed PCL/β-TCP scaffolds is the fact that many cells pass from porosities during in vitro cell seeding, leading to poor initial cell attachment. This study aimed to demonstrate the fabrication of a new collagen coating process for optimizing the hydrophilic property and cell-substrate interactions. This method may be used for coating collagen on any relevant biomedical constructs made of synthetic polymers to increase their biocompatibility and cell attachment. Materials and methodsPorous composite scaffolds fabricated by 3D printing were coated with collagen by a novel method and compared to traditional methods. After plasma treatment, samples were inverted in a homogenized collagen solution, freeze-dried, stabilized by crosslinking, freeze-dried again, and fibrillated using a defined salt concentration. Samples were characterized by a 3D laser microscope, cytocompatibility assay, attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopy, water absorption, protein absorption, and bioactivity assay. ResultsHomogenized collagen at pH= 7 resulted in a very uniform layer on the surface of scaffolds with significantly higher cell proliferation (p < 0.05). Collagen-coated scaffolds showed significantly higher water absorption, protein absorption, and bioactivity compared to non-coated samples (p < 0.05). ConclusionThe results demonstrate that both the pH and collagen structure influence the coating of scaffolds, while the concentrations used in this study do not have a significant difference in this aspect. The combination of homogenization and fibrillization makes scaffolds more biocompatible and desirable for bone tissue engineering.

Preparation and Characterization of Poly(lactide-co-glycolide-co-ε-caprolactone)- 1,4-Butanediamine-Modified Poly(lactide-co-glycolide)/Nano-Biaoactive Glass-β-Tricalcium Phosphate Composite Scaffolds

Polymer/bioceramic composite scaffolds have gained importance in treating bone defects. In this paper we described a novel degradable scaffold material for bone tissue repairing prepared by combining poly(lactide-co-glycolide-co-ε-caprolactone) (PLLGC) and 1,4-butanediamine-modified poly(lactide-co-glycolide) (BMPLGA) with nano-biaoactive glass (NBAG) and nano β-tricalcium phosphate (β-TCP). The composite scaffolds were characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) techniques. The FTIR results indicated that the inorganic fillers of NBAG and β-TCP were successfully incorporated into the PLLGC/BMPLGA matrix. The influences of the inorganic filler content and freezing temperature on the porosity and compressive strength of the composite scaffolds were studied. The composite scaffolds showed an interconnected pore structure with the pore size ranging from 20 to 100 μm depending on the freezing temperature and the inorganic fillers. The compressive strength was enhanced with increasing content of the inorganic fillers and the freezing temperature; however, the porosity decreased. We suggest the PLLGC-BMPLGA/NBAG-β-TCP composite scaffolds have great potential for application in bone tissue regeneration.

Integrity of the ECM Influences the Bone Regenerative Property of ECM/Dicalcium Phosphate Composite Scaffolds.

Due to the limitation of clinical autologous bone supply and other issues, the development of bone regeneration materials is still a hot topic. Natural tissue-derived bone repair materials have good biocompatibility and degradability, but their structure and properties are likely to be adversely affected during terminal sterilization. In this study, a composite scaffold consisting of the acellular extracellular matrix and dicalcium phosphate (ECM/DCP) was fabricated and terminally sterilized by γ-ray irradiation. In addition, the ECM/DCP scaffold was saturated with water and was also sterilized by γ-ray irradiation (RX-ECM/DCP). Results showed that the triple helix structure of collagen was better maintained in RX-ECM/DCP than in ECM/DCP. The thermal stability of RX-DCP/ECM was much better than that of ECP/ECM. The in vitro and in vivo performances of both types of scaffolds were also evaluated. The RX-ECM/DCP scaffold exhibited better in vitro bioactivity than that of the ECM/DCP scaffold as evidenced by more mineral formation in the simulated body fluid. In addition, RX-ECM/DCP also induced more effective bone regeneration than the ECM/DCP scaffold did in a rat calvarial defect model. Results sufficiently demonstrated that the addition of water to the scaffold could protect the structure of the ECM/DCP scaffold from being damaged by γ-ray irradiation during the terminal sterilization process. In summary, this study demonstrated a means to protect the ECM structure, which in turn led to the improvement of bone regenerative properties of the materials during γ-ray irradiation of ECM-based bone repair materials.

Mechanical properties and biocompatibility of MgO / Ca3(PO4)2 composite ceramic scaffold with high MgO content based on digital light processing

Magnesium oxide-calcium phosphate (MgO/Ca3(PO4)2) composite ceramic materials are considered a promising class of bioactive materials, expected to be used in artificial bone scaffolds. However, there are few pieces of research on the content of magnesium oxide in composite ceramic scaffolds. To study the effect of magnesium oxide content on the biocompatibility and mechanical properties of magnesium oxide-calcium phosphate composite ceramic scaffolds, six groups of scaffolds with magnesium oxide content of 10 wt%, 20 wt%, 30 wt%, 40 wt%, 100 wt%, and 0 wt% were produced by digital light processing (DLP) printing technology. And scaffolds’ pores size and porosity percentage were 0.6–1 mm and 50%, respectively. The compressive strength of the scaffold increased with the magnesium oxide proportion, and the 40 wt% group was almost twice that of the 0 wt% magnesium oxide group. The 40 wt% and 0 wt% magnesium oxide groups performed better for biocompatibility. Comprehensive analysis of the biocompatibility and mechanical properties of the scaffold confirmed that the 40 wt% magnesium oxide group was the best. The results show that high magnesium oxide content enhanced the mechanical properties and achieved well biocompatibility of the composite scaffolds, broadening the scope of future experimental research.

Toughening robocast chitosan/biphasic calcium phosphate composite scaffolds with silk fibroin: Tuning printable inks and scaffold structure for bone regeneration

The present work aims the production of composite bioceramic scaffolds by robocasting suppressing sintering as post printing process. To achieve this purpose, extrudable ink compositions containing a high concentration of bioceramic powders (hydroxyapatite and β-tricalcium phosphate) embedded in aqueous polymeric solutions of chitosan and silk fibroin were fine-tuned. Polymeric solutions of chitosan/silk fibroin with different ratios were tested, maintaining the total amount of bioceramic solids at 30 vol%. The inks were characterized by rheological studies in viscometry and oscillatory modes, being the printable ones selected to produce scaffolds with different macropore sizes (300 μm and 500 μm). The scaffolds were characterized by mechanical properties (dry and wet conditions) and morphological features, as well as its degradability. In vitro studies were also evaluated in the scaffolds that presented the best structural performance.The addition of 2 wt% silk fibroin to a 5 wt% chitosan matrix allows to significantly improve the mechanical performance of the printed composite scaffolds, reflected in high values for Young's modulus and maximum compressive strength. This trend was continued in wet scaffolds with a concomitant reduction of mechanical properties. Regarding degradability, the scaffolds in general presented a weight loss in the range of 14–18% after 28 days incubation in HEPES solution at two different pH values at 37 °C, with an associated release of calcium and phosphorus ions. The scaffold with 300 μm porosity comprising the both polymers in its composition presented the less rate degradation when compared to the scaffolds with similar porosity and containing only chitosan as base matrix. Moreover, the combined natural polymers gave rise to a significant increase in the metabolic activity of human osteoblasts grown on the scaffolds with both macropore' size, being in line with the full cellular filling of their surfaces, demonstrated by SEM and confocal imaging. The advances presented in this work are a promising path in the ink's development for extrusion-based additive manufacturing techniques and subsequent biomaterials, encompassing suitable physical and chemical characteristics with high potential to be used as bone substitutes.

Multifunctional silicon calcium phosphate composite scaffolds promote stem cell recruitment and bone regeneration.

A scaffold is one of the most significant implants for treating bone injury, while the precise control of stem cell proliferation and differentiation within a scaffold is still challenging. In this work, a composite scaffold was designed to be capable of recruiting endogenous stem cells, stimulating osteogenic differentiation and achieving significant bone repair function. The designed SiCP + SF@PFS silica-calcium phosphate composite scaffold was obtained by mixing the peptide PFS containing silk fibroin solution with the SiCP scaffold, and treating with horseradish peroxidase and H2O2. The results showed that the composite scaffold was able to release the PFS peptide continuously to induce the migration of mesenchymal stem cells. Meanwhile, cell proliferation and osteogenic differentiation were also improved after being seeded on the scaffold. In the cranial defect rat model, the composite scaffold was able to recruit CD29+ and CD90+ cells one week after implantation around the injury sites. The results of Micro-CT, H&E staining, Masson's staining and immunohistochemical staining indicated that the composite scaffold was able to promote new bone formation significantly.

Preparation of Porous Poly(Lactic Acid)/Tricalcium Phosphate Composite Scaffolds for Tissue Engineering

The development of bioactive and composite materials for tissue engineering applications is being investigated worldwide. Many approaches have been published by including combinations of resorbable polymers with hydroxyapatite (HA), tricalcium phosphate (TCP), bioactive glasses and glass-ceramics in different scaffolds architectures. Taking into account these antecedents, porous polylactic acid (PLA)/TCP composites were fabricated by employing dissolution-leaching technic from PLA/chloroform solution (10, 15, and 20 wt % of TCP). Composite scaffolds exhibited porosity values 1.3 times higher when compared to PLA foams. Their bioactive response of the composite foams after immersion in a simulated body fluid (SBF) was studied by X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR-ATR). By XRD analysis, diffraction peaks attributed to hydroxyapatite deposition were observed; and by FTIR-ATR, new absorption bands corresponding to HA were detected. Regarding mechanical properties, an increasing tendency on elastic Young's modulus values was observed at higher TCP concentrations. These results envision the feasibility of using these composites as precursors for bone tissue materials engineering.

Restoration of critical defects in the rabbit mandible using osteoblasts and vascular endothelial cells co-cultured with vascular stent-loaded nano-composite scaffolds

The success of large bone defect repair with tissue engineering technology depends mainly on angiogenesis and osteogenesis. In this study, we prepared poly-caprolactone/nano-hydroxyapatite/beta-calcium phosphate (PCL/nHA/β-TCP) composite scaffolds loaded with poly-(lactic-co-glycolic acid)/nano-hydroxyapatite/collagen/heparin sodium (PLGA/nHA/Col/HS) nanofiber small vascular stent by electrospinning and hot press forming-particle leaching methods. Supramolecular electrostatic self-assembly technology was used to modify the surfaces of small vascular stents to aid in hydrophilicity and anticoagulation. The surfaces of composite scaffolds were modified with an Arg-Gly-Asp (RGD) short peptide by physical adsorption to supply cell adhesion sites. The scaffolds were then combined with rabbit bone marrow-derived osteoblasts (OBs) and rabbit bone marrow-derived vascular endothelial cells (RVECs) to construct large, biologically active vascularized tissue-engineered bone in vitro; this bone was then used to repair critical bone defects in rabbit mandibles. Mechanical and biocompatibility testing results showed that PCL/nHA/β-TCP composite scaffolds loaded with small vascular stents had good surface structure, mechanical properties, biocompatibility, and bone-regeneration induction potential. Twelve weeks after implantation, histological analysis and X-ray scans showed that the use of osteoblasts and vascular endothelial cells co-cultured with PCL/nHA/β-TCP scaffolds was sufficient to repair critical defects in rabbit mandibles.

Effect of glutaraldehyde on the characteristics of chitosan–gelatin–β-tricalcium phosphate composite scaffolds

A composite scaffold was successfully fabricated using β-tricalcium phosphate (βTCP), which is extracted from limestone by first sintering and then reacting it with phosphoric acid through wet precipitation method. The resultant substance is then mixed with chitosan and gelatin. This novel method utilizes limestone, which is abundant in nature. This study optimizes the composite scaffold fabrication by using βTCP from limestone and evaluating the effect of glutaraldehyde on scaffold characteristics. The freeze-drying method was used to obtain a porous scaffold. The compressive strength of the cross-linked scaffolds (3.3 ± 0.3 MPa) was significantly higher than that of scaffolds without glutaraldehyde (1.7 ± 0.2 MPa). In contrast, the porosity of the cross-linked scaffolds was lower (85.8 ± 0.8 %) than the non-cross-linked scaffolds (89.1 ± 0.4 %). It is clear that the porosity had a considerable impact on the compressive strength, wherein lower porosity led to a higher compressive strength. In conclusion, glutaraldehyde is an effective cross-linker for the fabrication of chitosan–gelatin–βTCP composite scaffolds and significantly improves their compressive strength.

Development of carboxymethylcellulose based composites for bone tissue engineering

The present study focuses on the development of carboxymethylcellulose (CMC)–biphasic calcium phosphate (BCP) composite scaffolds through the freeze-drying process for bone tissue engineering applications. Citric acid or fumaric acid was added as the cross-linker of CMC to improve the stability of composite scaffolds. The effect of change in freezing temperature (−20, −40 or −80°C) on the pore morphology, swelling ability and mechanical properties of composite scaffolds was studied. Cross-linked scaffolds showed an increased thermal degradation temperature compared with non-cross-linked scaffolds. All the composite scaffolds showed a porous structure with homogeneous blending of CMC and BCP. Cross-linked scaffolds showed appreciable swelling ability and stability in phosphate-buffered saline, while non-cross-linked scaffolds were unstable for 24 h. Cross-linked scaffolds had lower compressive strength than non-cross-linked scaffolds under dry conditions. However, in the hydrated state, only citric acid-cross-linked scaffolds were stable with improved compressive strength of 64 ± 4, 57 ± 4 and 67 ± 4 kPa when processed at −20, −40 and −80°C, respectively. Furthermore, three-dimensional culture of Saos-2 cells on citric acid-cross-linked scaffolds showed their suitability for cell proliferation and osteogenic differentiation. Therefore, citric acid-cross-linked CMC–BCP composite scaffolds may be promising scaffolds for bone tissue engineering applications.