Phocid Seals Research Articles

High prevalence and low intensity of Echinophthirius horridus infection in seals revealed by high effort sampling

Seal lice (Echinophthirius horridus) are bloodsucking ectoparasites of phocid seals and vectors of pathogens like the heartworm, Acanthocheilonema spirocauda. Grey and harbour seal populations are recovering in German waters and wildlife health surveillance is crucial for wildlife conservation. A new, high effort sampling protocol for seal lice was applied for grey and harbour seals along the German North- and Baltic Sea coast. Freshly dead seals were systematically sampled within a health monitoring of stranded seals over 12 months. Prevalence, intensity and distribution patterns of seal lice were analysed. 58% of harbour seals (n = 71) and 70% of grey seals (n = 10) were infected with seal lice. A majority of harbour seals displayed mild levels of infection, while three were moderately and two were severely infected. The head was the preferred predilection site, indicating that E. horridus prefers body areas with frequent access to atmospheric oxygen. Nits and different developmental stages were recorded in all age classes in grey and harbour seals in all seasons. For the first time, copulating specimens of E. horridus were recorded on a dead harbour seal, highlighting that E. horridus reproduces throughout the year on seals of all age classes in German waters.

Osteological profiling of femoral diaphysis and neck in aquatic, semiaquatic, and terrestrial carnivores and rodents: effects of body size and locomotor habits

The increased limb bone density documented previously for aquatic tetrapods has been proposed to be an adaptation to overcome buoyancy during swimming and diving. It can be achieved by increasing the amount of bone deposition or by reducing the amount of bone resorption, leading to cortical thickening, loss of medullary cavity, and compaction of trabecular bone. The present study examined the effects of locomotor habit, body size, and phylogeny on the densitometric, cross-sectional, and biomechanical traits of femoral diaphysis and neck in terrestrial, semiaquatic, and aquatic carnivores, and in terrestrial and semiaquatic rodents (12 species) by using peripheral quantitative computed tomography, three-point bending, and femoral neck loading tests. Groupwise differences were analyzed with the univariate generalized linear model and the multivariate linear discriminant analysis supplemented with hierarchical clustering. While none of the individual features could separate the lifestyles or species adequately, the combinations of multiple features produced very good or excellent classifications and clusterings. In the phocid seals, the aquatic niche allowed for lower femoral bone mineral densities than expected based on the body mass alone. The semiaquatic mammals mostly had high bone mineral densities compared to the terrestrial species, which could be considered an adaptation to overcome buoyancy during swimming and shallow diving. Generally, it seems that different osteological properties at the levels of mineral density and biomechanics could be compatible with the adaptation to aquatic, semiaquatic, or terrestrial niches.

Multi-cusped postcanine teeth are associated with zooplankton feeding in phocid seals

Tooth morphologies often reflect diet in animals. Among marine mammals, a well-known example is the krill-feeding crabeater seal Lobodon carcinophaga, in which complex, comb-like postcanine teeth function as a sieve by retaining krill inside the mouth while expelling water. However, information on tooth morphology and function is scarce for other seal species. A recent bio-logging study found that Baikal seals Pusa sibirica feed on tiny pelagic amphipods at remarkably high rates with highly multi-cusped postcanine teeth, highlighting the need for comparative analyses on tooth morphologies and diets in phocid seals. Here, we quantified postcanine tooth morphology for 13 seal species based on museum skull specimens, with a particular focus on Baikal seals and their related species (genera Pusa and Phoca). Pusa species, including Baikal seals, had more specialized multi-cusped postcanine teeth than Phoca species, reflecting higher zooplankton proportions in their diets. Postcanine teeth of Baikal seals exhibited the highest degree of specialization among Pusa, even when the effect of age-related wear was controlled for. This result agrees with the highest zooplankton preference in this species. Further, we found a strong positive correlation between the degree of specialization in postcanine teeth and zooplankton reliance across phocid seal species. Our findings indicate that the functional role of multi-cusped postcanine teeth as a sieve is not limited to crabeater seals but prevails in many phocid seals feeding on zooplankton.

Undulating Seal Whiskers Evolved Optimal Wavelength-to-Diameter Ratio for Efficient Reduction in Vortex-Induced Vibrations.

Seals are well-known for their remarkable hydrodynamic trail-following capabilities made possible by undulating flow-sensing whiskers that enable the seals to detect fish swimming as far as 180m away. In this work, the form-function relationship in the undulating whiskers of two different phocid seal species, viz. harbor and gray seals, is studied. The geometry and material properties of excised harbor and grey seal whiskers are systematically characterized using blue light 3D scanning, optical and scanning electron microscopy, and nanoindentation. The effect of the undulating geometry on the whiskers' vibration in uniform water flow is studied using both experimental (piezoelectric MEMS and 3D-printed piezoresistive sensors developed in-house) and numerical (finite element method) techniques. The results indicate that the dimensionless ratio of undulation wavelength to mean whisker diameter (λ/Dm ) in phocid seals may have evolved to be in the optimal range of 4.4-4.6, enabling an order-of-magnitude reduction in vortex-induced vibrations (compared to a similarly-shaped circular cylinder) and, consequently, an enhanced flow sensing capability with minimal self-induced noise. The results highlight the importance of the dimensionless λ/Dm ratio in the biomimetic design of seal whisker-inspired vibration-resistant structures, such as marine risers and wake detection sensors for submarines.

The contribution of abiotic markers in interpreting the taphonomic weathering of Homiphoca long-bones from Langebaanweg, South Africa

We examine weathering on early Pliocene Homiphoca capensis long-bones discovered in deposits from Langebaanweg (West Coast Fossil Park) coastal site, Western Cape, South Africa. Thus far, taphonomic research of phocid seal remains retrieved from ‘E’ Quarry have focused on biotic markers. The abiotic taphonomic study is limited and reliant upon the Weathering Series classified by Behrensmeyer; the current weathering base stages do not, however, reflect the abiotic weathering progression we found preserved in a sample of 653 Homiphoca long-bones. Utilizing Behrensmeyer's definition for early weathering stages (0 and 1), we recognized five new substages within the palaeoenvironment of ‘E’ Quarry and applied them to the Homiphoca long-bones. We suggest that this contribution could be applied to other fossil assemblages with a view to addressing some current biases and furthering understanding of depositional histories.

California sea lion (Zalophus californianus) lymph-node explant reveals involvement and possible transcriptional regulation of SLAM and nectin-4 during phocine distemper virus infection.

Phocine distemper virus (PDV) is a significant cause of mortality for phocid seals; however, the susceptibility of otariids to this virus is poorly understood. The authors used a lymph-node explant culture system from California sea lions (Zalophus californianus, CSL) to investigate: (1) the role of signaling lymphocyte activation molecule (SLAM) and nectin-4 in PDV infection and their cellular expression patterns, (2) if PDV induces transcriptional regulation of cell-entry receptors, and (3) the involvement of apoptosis in PDV infection. PDV replicated in the lymph-node explants with peak replication 3 days post-infection (dpi), but the replication was not sustained 4 to 5 dpi. The PDV+ cells co-localized SLAM and nectin-4. These cells expressed IBA1, indicating a histiocytic lineage. Comparison of receptor expression between infected and mock-infected lymph nodes suggested transcriptional downregulation of both receptors during the initial stage of infection and upregulation during the late stage of infection, but the values lack of statistical significance. Cleaved caspase-3+ cells were slightly increased in the infected lymph nodes compared with the mock-infected lymph node from 1 to 4 dpi, but without statistical significance, and a few apoptotic cells co-expressed PDV. The results suggest that lymph-node explants might be an important model to study PDV pathogenesis. CSLs have the potential to be infected with PDV, as they express both cell-entry receptors in histiocytes. The lack of statistical significance in the PDV replication, transcriptional regulation of viral receptors, and changes in apoptosis suggest that although CSL might be infected by PDV, they might be less susceptible than phocid species.

Exposure of wild Caspian seals (Pusa caspica) to parasites, bacterial and viral pathogens, evaluated via molecular and serological assays

Disease surveillance of marine mammal populations is essential to understand the causes of strandings, identify potential threats to animal health, and to support development of conservation strategies. Here we report the first large multi-pathogen screening of prevalence for viruses, bacteria and parasites in a sample of 177 live, healthy, wild Caspian seals (Pusa caspica), captured and released during satellite telemetry studies 2007-2017. Employing molecular and serological assays we assess prevalence of pathogens known to be of significance for marine mammal health worldwide, and evaluate the results in relation to Caspian seal health and conservation. RT-PCR, and PCR assays find evidence for infection by Canine Distemper Virus (CDV), Phocine herpes virus, phocine adenovirus and Influenza A at prevalences of 5%, 6.4%, 21.7%, and 4% respectively. The genomes of CDV isolates collected in 2008 showed 99.59% identity with the 2000 Caspian seal CDV epizootic strain. A partial coding sequence for the Us2 gene from the Caspian seal herpes virus was identical to PhHV-1 isolate PB84, previously reported from a harbor seal (Phoca vitulina), while amplicon sequences for the adenovirus polymerase gene indicated a novel strain. ELISA assays detected exposure to Influenza A (55% of tested samples), adenovirus (25%), coronavirus (6%), CDV (8%), herpes virus (94%), Toxoplasma gondii (2.6%) and heartworm (1%). Hemagglutination inhibition (HI) tests detected exposure to Influenza B at a prevalence of 20%, and Leptospira microscopic agglutination tests detected suspected exposure to Leptospira serovars in 9% of tested samples. Overall, the risks, profile and prevalence of pathogens in Caspian seals appear comparable to other wild phocid seal populations. Our results suggest Caspian seals have exposure pathways to pathogens with epizootic potential or ability to cause significant morbidity, and that disease impacts could reduce the resilience of the population to other conservation threats. Caspian seals are listed as Endangered by the International Union for Conservation of Nature (IUCN), and we recommend that resources are invested to support further surveillance programs and to understand how anthropogenic pressures may influence future disease risks. A translated version of this abstract is available in Russian and Kazakh in the Supplementary Material (Presentation 1 and Presentation 2)

New cetacean fossils from the late Cenozoic of South Africa

Marine sediments from the western coast of southern Africa record the origin of the Benguela Upwelling System, one of the most productive in the world. High productivity, in turn, is reflected in a diverse marine mammal fossil assemblage, comprising whales, dolphins and a phocid seal. Here, we describe new records of baleen whale (mysticete) fossils from the early Pliocene localities of Saldanha Steel, Milnerton and Langebaanweg, as well as several potentially younger specimens trawled from offshore sediments. The presence of the extinct rorquals Diunatans and Fragilicetus suggests biogeographical links with the eastern North Atlantic and, thus, potentially antitropical population structuring. The trawled specimens also include rorquals (e.g., the blue whale, Balaenoptera cf. musculus), as well as a right whale (Eubalaena) and a pygmy right whale (Caperea). The latter is the first fossil of this family every discovered in Africa, and only the seventh specimen to be reported worldwide.

Ladoga Ringed Seal (Pusa hispida ladogensis) Can Breed on Land: A Case Study of the Nursing Period

The ringed seal gives birth and nurses offspring in a subnivean lair in fast ice. Its breeding habitat is transforming under the impact of climate change. Here we report the outcome of an observation of a female freshwater Ladoga ringed seal (Pusa hispida ladogensis) and her pup during the 2020 breeding season, when less than 1% of Lake Ladoga was covered with ice. We located a newborn pup in a coastal zone of an island and tracked its survival on land using the camera trap method during daylight. Altogether, we captured 2978 photos, in which the seals were present in 637. The female nursed the pup at the birth site for 34 – 37 days, which is similar to the lactation period in lairs of the Arctic subspecies (36 – 41 days, 39 days on average). The female either stayed with the pup or spent time in prolonged aquatic bouts during the day. Percentage of suckling was in the range of 2.4% – 4.7% (mean 3.3%, SD = 1.1) on different days. Based on an additional video recording, we found that the pup’s behaviour was characterized by a high level of vigilance in comparison with openly breeding phocid seals. This case study indicates that the ringed seal in Lake Ladoga is able to nurse pups on land from soon after birth to pre-weaning. However, breeding success in warm springs can be constrained by predator pressure.

Wavy Whiskers in Wakes: Explaining the Trail-Tracking Capabilities of Whisker Arrays on Seal Muzzles.

Seals can detect prey up to 180 m away using only their flow-sensing whiskers. The unique undulating morphology of Phocid seal whiskers reduces vortex-induced vibrations (VIVs), rendering seals highly sensitive to biologically relevant flow stimuli. In this work, digital models of harbor and grey seal whiskers are extracted using 3D scanning and a mathematical framework that accurately recreates their undulating geometry is proposed. Through fluid-structure interaction studies and experimental investigations involving a whisker array mounted on 3D-printed microelectromechanical systems sensors, the vibration characteristics of the whisker array and the interaction between neighboring whiskers in steady flows and fish-wake-like vortices are explained for the first time. Results reveal that the downstream vortices intensity and resulting VIVs are consistently lower for grey than harbor seal whiskers and a smooth cylinder, suggesting that the grey seal whisker geometry can be an ideal template for the biomimetic design of VIV-resistant underwater structures. In addition, neighboring whiskers in an array influence one another by resulting in greater flow vorticity fluctuation and distribution area, thus causing increased vibrations than an isolated whisker, which indicates the possibility of a signal-strengthening effect in whisker arrays.

Mirror image serum lipid carrier protein profiles in pup and lactating mother Atlantic grey seals reflect contrasting resource mobilisation challenges

True, phocid seals have the shortest known lactations relative to body mass, during which mass transfer of adipose stores from mother to offspring occurs at an unrivalled rate and extent. The mothers of most species of seal fast until weaning whilst their pups gorge on the most fat-rich milks known. This results in a dramatic reduction in maternal blubber mass while pups may triple their body weights before weaning. Mothers mobilise their blubber fat, transport it via blood to their mammary glands and into milk, whilst pups transfer fat in the opposite direction, from their intestines, via blood, to their blubber. Using proteomic analysis of mother and pup sera from Atlantic grey seals, we find that this mirror image flux of lipids between mothers and pups is reflected in an almost inverse relationship in the proteins in their blood specialised to transport fats, lipids, and fat-soluble vitamins. For instance, apolipoproteins ApoB-48/100, ApoA-II and ApoA-IV, which are structural components of the main lipid carrier complexes such as chylomicrons and HDL particles, occur at much higher levels in pups than mothers. Meanwhile, carriers of fat-soluble vitamins such as retinol- and vitamin D-binding proteins are lower in pups and gradually build towards weaning. In contrast, sex hormone-binding globulin occurs at remarkably high relative concentrations in pups. There are therefore dramatic differences between, and an unrealised complexity in, the balance of proteins involved in the rapid transfer of fats and other lipids from mother to pups in preparing their offspring for their post-weaning fasts on land and eventual survival at sea before they can feed again.

3D Printed Graphene Piezoresistive Microelectromechanical System Sensors to Explain the Ultrasensitive Wake Tracking of Wavy Seal Whiskers

AbstractMany marine animals perform fascinating survival hydrodynamics and perceive their surroundings through optimally evolved sensory systems. For instance, phocid seal whiskers have undulations that allow them to resist noisy self‐induced vortex‐induced vibrations (VIV) while locking their vibration frequencies to wakes generated by swimming fishes. In this study, fully 3D‐printed microelectromechanical systems (MEMS) sensors with high gauge factor graphene nanoplatelets piezoresistors are developed to explain the exquisite sensitivity of whisker‐inspired structures to upstream wakes. The sensors are also used to measure natural frequencies of excised harbor (Phoca vitulina) and grey (Halichoerus grypus) seal whiskers and determine the effect of whisker orientation on the VIV, which can explain the possible natural orientation of whiskers during active hunting. Experimental investigations conducted in a recirculating water flume show that whisker‐inspired sensors successfully sense an upstream wake located up to 10× the whisker diameter by locking to the frequency of the wake generator, thus mimicking the sensing mechanism of the seal whisker. The combination of VIV reduction and frequency‐locking with the upstream wake generator demonstrates the whisker‐inspired sensor's high signal‐to‐noise ratio, indicating its efficiency in long‐distance wake sensing as well as its potential as an alternative to visual and acoustic sensors in underwater robots.

Novel 3D in situ visualization of seal heartworm (Acanthocheilonema spirocauda) larvae in the seal louse (Echinophthirius horridus) by X-ray microCT

The seal heartworm Acanthocheilonema spirocauda (Nematoda: Onchocercidae) parasitizes the heart and pulmonary arteries of various phocid seals of the Northern Hemisphere. Over many decades, potential vectors of this parasite have been discussed, and to this date, the life cycle is not fully known. The seal louse Echinophthirius horridus (Anoplura: Echinophthiriidae) is an obligatory, permanent and haematophagous ectoparasite of phocids that has been hypothesized to function as obligate intermediate host for A. spirocauda. We examined 11 adult E. horridus specimens collected from stranded harbour seals (Phoca vitulina) in rehabilitation at the Sealcentre Pieterburen by X-ray microCT imaging, aiming to illustrate larval A. spirocauda infection sites in situ. In three of these specimens, thread-like larvae were detected in insect organs. Detailed imaging of the most infected louse revealed a total of 54 A. spirocauda larvae located either in fat bodies or the haemocoel. Histological analysis of the same specimen illustrated nematode cross-sections, confirming X-ray microCT data. The current data strongly suggest that E. horridus is a natural intermediate host for A. spirocauda. Moreover, we demonstrate the potential of X-ray microCT-based imaging as a non-destructive method to analyze host-parasite interactions, especially in the neglected field of marine mammal parasitology.

Thresholds for noise induced hearing loss in harbor porpoises and phocid seals

Intense sound sources, such as pile driving, airguns, and military sonars, have the potential to inflict hearing loss in marine mammals and are, therefore, regulated in many countries. The most recent criteria for noise induced hearing loss are based on empirical data collected until 2015 and recommend frequency-weighted and species group-specific thresholds to predict the onset of temporary threshold shift (TTS). Here, evidence made available after 2015 in light of the current criteria for two functional hearing groups is reviewed. For impulsive sounds (from pile driving and air guns), there is strong support for the current threshold for very high frequency cetaceans, including harbor porpoises (Phocoena phocoena). Less strong support also exists for the threshold for phocid seals in water, including harbor seals (Phoca vitulina). For non-impulsive sounds, there is good correspondence between exposure functions and empirical thresholds below 10 kHz for porpoises (applicable to assessment and regulation of military sonars) and between 3 and 16 kHz for seals. Above 10 kHz for porpoises and outside of the range 3-16 kHz for seals, there are substantial differences (up to 35 dB) between the predicted thresholds for TTS and empirical results. These discrepancies call for further studies.

Is a Little Enough? Paucity of Immune Proteins in Serum of Precocial Neonates of a Marine Carnivoran—the Atlantic Grey Seal

Mammalian mothers usually provide their offspring with large quantities of immunoglobulins (antibodies) for circulation in blood, either trans-placentally before birth, via colostrum briefly thereafter, or, less commonly, from milk. Neonates of true, phocid seals, however, are peculiarly impoverished in serum immunoglobulins, the levels of which slowly increase but do not reach adult levels by the time of weaning. We investigated whether grey seal (Halichoerus grypus) neonates compensate through an elevation or rapid maturation in levels of serum innate immune factors, namely acute phase and complement proteins. Instead, their sera contained remarkably low levels of acute phase proteins (including C-reactive protein, haptoglobin, hemopexin, ceruloplasmin, orosomucoid), compared to their mothers, that barely increased to adult levels by weaning. For complement, there was a strong demarcation between the early activation and amplification cascade components (present at normal adult levels in pups) and the late lytic membrane attack complex and regulatory proteins (consistently at low relative levels). Phocid neonates therefore differ dramatically from land Carnivorans, such as dogs and cats, in early life immune protection. That neonatal phocids survive this apparent vulnerability to infections between birth and weaning prompts questions as to what other mechanisms protect them, and the adaptive value of their seeming vulnerability.

Flow-signal correlation in seal whisker array sensing

Phocid seals detect and track artificial or biogenic hydrodynamic trails based on mechanical signals of their whisker arrays. In this paper, we investigated the correlations between flow structures and whisker array signals using a simplified numerical model of fluid–structure interaction (FSI). Three-dimensional (3D) wakes of moving paddles in three different shapes (rectangular plate, undulated plate, and circular cylinder) were simulated using an in-house immersed-boundary-method-based computational fluid dynamics solver. One-way FSI was then simulated to obtain the dynamic behavior and root signal of each whisker in the two whisker arrays on a seal head in each wake. The position, geometry, and material properties of each whisker were modeled based on the measurements reported in literatures. The correlations between the wake structures and whisker array signals were analyzed. It was found that the patterns of the signals on the whisker arrays can reflect the strength, timing, and moving trajectories of the jets induced by the vortices in the wakes. Specifically, the rectangular plate generates the strongest starting vortex ring as well as the strongest jets, while the undulated plate generates the weakest ones. These flow features are fully reflected by the largest whisker signal magnitude in the rectangular plate sensing and the smallest one in the undulated plate sensing. Moreover, the timing of the signal initiation and the maximum signal agree well with the timing of the jet reaching the arrays and the maximum flow speed, respectively. The correlation coefficient between the moving trajectories of the jet and the movement of the high signal level region in the array was found to be higher than 0.9 in the rectangular plate case. The results provide a physical insight into the mechanisms of seal whisker flow sensing.

Variation in body size of ringed seals (Pusa hispida hispida) across the circumpolar Arctic: evidence of morphs, ecotypes or simply extreme plasticity?

The ringed seal is a small phocid seal that has a northern circumpolar distribution. It has long been recognized that body size is variable in ringed seals, and it has been suggested that ecotypes that differ in size exist. This study explores patterns of body size (length and girth) and age-at-maturity across most of the Arctic subspecies’ range using morphometric data from 35 sites. Asymptotic lengths varied from 113 to 151 cm, with sites falling into five distinct size clusters (for each sex). Age-at-maturity ranged from 3.1 to 7.4 years, with sites that had early ages of sexual maturity generally having small length-at-maturity and small final body length. The sexes differed in length at some sites, but not in a consistent pattern of dimorphism. The largest ringed seals occurred in western Greenland and eastern Canada, and the smallest occurred in Alaska and the White Sea. Latitudinal trends occurred only within sites in the eastern Canadian Arctic. Girth (with length and season accounted for) was also highly variable but showed no notable spatial pattern; males tended to be more rotund than females. Genetic studies are needed, starting with the “giants” at Kangia (Greenland) and in northern Canada to determine whether they are genetically distinct ecotypes. Additional research is also needed to understand the ecological linkages that drive the significant regional size differences in ringed seals that were confirmed in this study, and also to understand their implications with respect to potential adaptation to climate change.

Fur seals do, but sea lions don't - cross taxa insights into exhalation during ascent from dives.

Management of gases during diving is not well understood across marine mammal species. Prior to diving, phocid (true) seals generally exhale, a behaviour thought to assist with the prevention of decompression sickness. Otariid seals (fur seals and sea lions) have a greater reliance on their lung oxygen stores, and inhale prior to diving. One otariid, the Antarctic fur seal (Arctocephalus gazella), then exhales during the final 50-85% of the return to the surface, which may prevent another gas management issue: shallow-water blackout. Here, we compare data collected from animal-attached tags (video cameras, hydrophones and conductivity sensors) deployed on a suite of otariid seal species to examine the ubiquity of ascent exhalations for this group. We find evidence for ascent exhalations across four fur seal species, but that such exhalations are absent for three sea lion species. Fur seals and sea lions are no longer genetically separated into distinct subfamilies, but are morphologically distinguished by the thick underfur layer of fur seals. Together with their smaller size and energetic dives, we suggest their air-filled fur might underlie the need to perform these exhalations, although whether to reduce buoyancy and ascent speed, for the avoidance of shallow-water blackout or to prevent other cardiovascular management issues in their diving remains unclear. This article is part of the theme issue 'Measuring physiology in free-living animals (Part I)'.

The seal louse (Echinophthirius horridus) in the Dutch Wadden Sea: investigation of vector-borne pathogens

BackgroundBelonging to the anopluran family Echinophthiriidae, Echinophthirius horridus, the seal louse, has been reported to parasitise a broad range of representatives of phocid seals. So far, only a few studies have focused on the vector function of echinophthiriid lice, and knowledge about their role in pathogen transmission is still scarce. The current study aims to investigate the possible vector role of E. horridus parasitising seals in the Dutch Wadden Sea.MethodsE. horridus seal lice were collected from 54 harbour seals (Phoca vitulina) and one grey seal (Halichoerus grypus) during their rehabilitation period at the Sealcentre Pieterburen, The Netherlands. DNA was extracted from pooled seal lice of individual seals for molecular detection of the seal heartworm Acanthocheilonema spirocauda, the rickettsial intracellular bacterium Anaplasma phagocytophilum, and the cell wall-less bacteria Mycoplasma spp. using PCR assays.ResultsSeal lice from 35% of the harbour seals (19/54) and from the grey seal proved positive for A. spirocauda. The seal heartworm was molecularly characterised and phylogenetically analysed (rDNA, cox1). A nested PCR was developed for the cox1 gene to detect A. spirocauda stages in seal lice. A. phagocytophilum and a Mycoplasma species previously identified from a patient with disseminated ‘seal finger’ mycoplasmosis were detected for the first time, to our knowledge, in seal lice.ConclusionsOur findings support the potential vector role of seal lice in the transmission of A. spirocauda and reveal new insights into the spectrum of pathogens occurring in seal lice. Studies on vector competence of E. horridus, especially for bacterial pathogens, are essentially needed in the future as these pathogens might have detrimental effects on the health of seal populations. Furthermore, studies on the vector role of different echinophthiriid species infecting a wide range of pinniped hosts should be conducted to extend the knowledge of vector-borne pathogens.Graphical

Maternal behavior and early behavioral ontogeny of the Mediterranean monk seal Monachus monachus in Greece

Understanding behavioral ontogeny is important for the successful conservation of endangered marine mammals. This is particularly the case for phocid seals, as during the early stages of their life they must acquire, largely independently, essential survival skills. We studied the maternal behavior and early behavioral ontogeny of the Mediterranean monk seal, one of the most endangered marine mammals on Earth, by installing a remote-controlled, infrared, video system in a pupping cave in Greece and recording the behavior of 2 adult females and their newborn pups (September 2007 to March 2008). Behavioral observations focused on the monitoring of individual attendance (i.e. percentage of time spent in attendance and attendance time) and the description of interactions. Following parturition, the percentage of time spent in attendance of both mothers and pups decreased gradually as pups developed towards independence. Overall, the pupping cave was used almost continuously by the 4 individuals for 3 to 4 mo. Similarly, attendance times also decreased after parturition. During the first 10 d postpartum, maternal attendance was followed by a 0.5 to 13.8 h absence, during which we presume that the adult females went out foraging. We also detail various in-cave interactions for the first time in Mediterranean monk seals in Greece, including lactations, interactions between mothers and pups, interactions between pups and general seal interactions. Our study increases our understanding of the in-cave behavior of the Mediterranean monk seal, while highlighting the vital role of suitable caves in the reproduction and survival of the species and the necessity to effectively protect this type of habitat.