Anaemia induced by oxidative agents like phenylhydrazine (PHZ) is often accompanied by hepatic and renal dysfunction due to increased oxidative stress and free radical generation. This study assessed the hepatoprotective and nephroprotective effects of Hibiscus rosa-sinensis (China rose) and Trigonella foenum-graecum (fenugreek) leaf extracts in PHZ-induced anaemic mice over a 60-day period. Mice treated with PHZ exhibited significantly elevated serum liver biomarkers—ALT (86.5 ± 3.6 U/L), AST (120.3 ± 4.2 U/L), ALP (210.7 ± 7.5 U/L), total bilirubin (1.85 ± 0.15 mg/dL), and direct bilirubin (0.75 ± 0.05 mg/dL)—along with decreased albumin levels (2.3 ± 0.2 g/dL), indicating hepatocellular damage. Kidney function parameters also showed marked derangement with increased serum creatinine (1.52 ± 0.08 mg/dL), blood urea nitrogen (BUN) (46.3 ± 3.1 mg/dL), and uric acid (5.8 ± 0.3 mg/dL). Treatment with China rose and fenugreek extracts, individually and in combination, significantly restored liver and kidney markers toward normal levels, with the combination group showing the most effective recovery (e.g., ALT: 42.1 ± 2.1 U/L; creatinine: 0.84 ± 0.05 mg/dL). The outcomes were comparable to those observed with standard ferrous sulfate treatment. These findings demonstrate the potent hepato-renal protective effects of China rose and fenugreek extracts, likely mediated through their antioxidant properties, highlighting their potential as natural therapeutic agents in anaemia management

This study prepared seven compounds I1-7 containing pyrrole, pyridine, and pyrazoline rings. First, four chalcones I1-4 were prepared as precursors, and then three pyrazoline I5-7 cores were prepared from them. The chalcones compounds were synthesized by reacting with 4-ethoxy acetophenone or 3,4-(Methylenedioxy) acetophenone with Pyrrole-2-carboxaldehyde and 2-Pyridinecarboxaldehyde. The pyrazoline ring derivatives were obtained from reaction chalcones with hydrazine derivatives: (hydrazine,2-hydrazinobenzothiazole& phenyl hydrazine). All compounds were proven by FTIR, 1H& 13C NMR spectroscopies in addition to the physical properties. The biological activity of some compounds against Leishmania tropica and human lymphocytes was examined using the 3-[4,5-dimethylthiazole]-2,5-diphenyltetrazolium bromide (MTT) test. Considering the Leishmania tropica test, the prepared compound I3 showed the highest inhibition of 80-81%, While compound I2 showed the highest toxicity against lymphocytes of 54-70% at (1 to 0.0625) mg/ml, which was confirmed by their molecular docking study with three different enzymes (DNA polymerase iota, HSP 90, Lysine-specific demethylase) has been measured and compared with the binding energy of the drug Amphotericin B, and Azithromycin where the docking energy appeared close to that of the drugs that were used, which nominates them as good anti-leishmania and lymphocytes in the future.

In response to the growing global threat of antimicrobial resistance, this work seeks to synthesize and analyze chalcone-derived pyrazoline derivatives and assess their antibacterial efficacy against the Staphylococcus aureus and Escherichia coli). A series of pyrazoline compounds were synthesized using both classical step-wise and one-pot synthetic strategies, involving Claisen–Schmidt condensation of 4-(4-fluorobenzyl) oxy acetophenone with various substituted benzaldehydes that subsequently undergo cyclization with phenyl hydrazine. The chemical structures of the produced chalcones and their corresponding pyrazolines were characterized using FTIR, ¹H-NMR, and ¹³C-NMR spectroscopy. Physicochemical characterization revealed the products were obtained in high yields and were sufficiently stable for isolation, with improved yields observed via the one-pot method. Antibacterial activity was assessed using the disk diffusion technique at multiple concentrations (200–800 ppm). The results demonstrated that pyrazoline derivatives exhibited significantly higher inhibition zones, particularly against S. aureus, compared to their chalcone precursors. Compounds 5a, 5c, and 5f had the most significant antibacterial efficacy, whereas chalcones showed minimal to no action against E. coli. The findings confirm the superior bioactivity of the pyrazoline ring system and suggest the crucial impact of electron- giving and electron-removing substituents on antibacterial potential. This research underscores one-pot synthesis as operationally simple and reducing waste generation by eliminating the need for intermediate isolation, thereby offering a more efficient and practical route, time-saving method for producing structurally varied, physiologically active pyrazolines, presenting attractive possibilities for the development of novel antibacterial medicines.

Investigation of antioxidant properties of Trigonella foenum-graecum (fenugreek) seed and Hibiscus rosa-sinensis (China rose) bark extracts in phenylhydrazine (PHZ)-induced anaemia models in mice. Ethanol extracts of both plant materials were prepared through maceration and evaluated for in-vitro antioxidant activity using DPPH radical scavenging, hydrogen peroxide scavenging, and reducing power assays. Results revealed dose-dependent antioxidant activity, with Hibiscus rosa-sinensis showing greater reducing power and Trigonella foenum-graecum demonstrating superior hydrogen peroxide and DPPH scavenging activity. In vivo, anaemia was induced in Swiss albino mice using PHZ (10 mg/kg body weight), leading to significant reductions in antioxidant enzyme levels including superoxide dismutase (SOD), reduced glutathione (GSH), and glutathione peroxidase (GSHPx). Treatment with individual and combined plant extracts significantly restored antioxidant parameters. Acute and sub-acute toxicity studies established the safety of both extracts up to 2000 mg/kg, and a combined dose of 400 mg/kg body weight was found effective and non-toxic. These findings support the therapeutic potential of T. foenum-graecum and H. rosa-sinensis as natural antioxidants with haemoprotective efficacy, particularly in oxidative stress-related anaemic conditions.

This study describes a series of water-soluble half-sandwich ruthenium(II), rhodium(III), and iridium(III) complexes with α-diimine ligands containing substituted aromatic groups. These ligands were derived from glyoxal and 2-aminophenol (a), 4-methyl-2-aminophenol (b), 4-aminophenol (c), phenyl hydrazine (d), and 1-aminonaphthalene (e). The ruthenium(II) (1b–1e), rhodium(III) (2a–2c, 2e), and iridium(III) complexes (3a–3e) were obtained by reacting the ligands (a–e) with the corresponding dimeric precursor [(η6-p-cym)RuCl2]2 (p-cym = p-cymene) or [(η5-Cp*)MCl2]2 (Cp* = pentamethylcyclopentadienyl, M = Rh, Ir) in air and under nonanhydro conditions. The air-stable and water-soluble ruthenium(II), rhodium(III), and iridium(III) complexes were characterized via nuclear magnetic resonance spectroscopy and electrospray ionization–mass spectrometry. The structures of complexes [(η6-p-cym)Ru(d)Cl]Cl, 1d; [(η5-Cp*)Ir(a)Cl]Cl, 3a; and [(η5-Cp*)Ir(c)Cl]Cl, 3c were determined via single-crystal X-ray diffraction. Additionally, the complexes exhibited catalytic activity as precatalysts in formic acid decomposition. Complex [(η5-Cp*)Ir(d)Cl]Cl, 3d achieved turnover number (TON) and turnover frequency (TOF) values of up to 2150 and 3861 h−1, respectively, at short reaction times. In the hydrogenation of carbon dioxide, [(η6-p-cym)Ru(e)Cl]Cl, 1e attained TON and TOF values of up to 1385 and 69.25 h−1, respectively.

New insight into role of mast cells in erythrocyte homeostasis and clearance under oxidative stress conditions in vivo

In this study, we report NNN pincer bis-imino pyridine-supported copper(II) catalysts for the sustainable, eco-friendly, and practical multi-component synthesis (MCS) of pyrazolines and pyrimidines driven by the acceptorless dehydrogenation of benzyl alcohols. Herein, we synthesize and characterize two well-defined phosphine-free NNN pincer-supported copper(II) complexes, C1 and C2, using IR, UV-vis, HRMS, and single-crystal XRD. Utilizing these complexes, we develop the first multi-component synthetic route for 1,3,5-trisubstituted pyrazolines (TriPyz) from the dehydrogenative coupling of renewable benzyl alcohols and aromatic ketones with phenyl hydrazine, generating ecologically benign H2O and H2 as side products. The catalytic potential of C1 and C2 is further extended for the MCS of 2,4,6-trisubstituted pyrimidines (TriPym) using primary alcohols, aromatic ketones, and amidine hydrochloride. Following the current methodology, we explore a broad range of TriPyz (30 examples) and TriPym (37 examples) with isolated yields of up to 94%. A series of control experiments and HRMS analysis of the catalytic cycle are carried out to reveal the possible reaction mechanism involving Cu(II)-hydride as a key intermediate.

Cancer is among the leading causes of death in the world today. Several strategies were used to control cancer including chemotherapy, radiotherapy and targeted therapy. Among the problems related to antiproliferative chemotherapy against cancer is the medication resistance which require continuous generation of new agents. These new potential agents could be extracted from natural sources or prepared synthetically. This research represents the preparation of new heterocycles incorporating an indenopyridazine nucleus as potential antiproliferative agents. The synthetic strategy was initiated by the treatment of ninhydrin with cyanoacetohydrazide resulting in the formation of the key intermediate indeno[2,1-c]pyridazine scaffold 4, which undergoes condensation with various nitrogenous nucleophilic reagents (namely: phenyl hydrazine, substituted benzohydrazide, and thiosemicarbazide). Cyclization of thiosemicarbazone derivative 8 with ethyl bromoacetate furnished the target compound thiazolin-4-one derivative 9, which was condensed with three 5-aryl-1H-pyrazole-4-carbaldehydes to furnish the corresponding 5-arylidene-thiazolin-4-one derivatives 11a-c. In addition, thiosemicarbazone derivative 8 undergoes reactions with α-chloroesters 12 or α-chloroketones 14 to yield the indenopyridazine-thiazole hybrids 13 and 15, respectively. The cytotoxic activity of the targeted indeno[2,1-c]pyridazine compounds was explored against four cancer cell lines invitro. Indenopyridazine-thiazolin-4-one hybrid 11c exhibited the highest cytotoxicity against HepG2 (IC50 = 7.43 μM) and MCF-7 cells (IC50 = 4.37 μM). Their IC50 values were very close to the reference drug 5-fluorouracil. Also, compound 7b was found to show a very strong cytotoxic effect against HepG2 (IC50 = 10.20 μM) and MCF-7 (IC50 = 7.39 μM). Moreover, molecular docking indicated that compounds 11b, 7b, and 11c had the best docking score against vascular endothelial growth factor recetor-2 (VEGFR-2) which is the potential target predicted by target fishing.

This study investigates green chemistry methods to design the indole derivatives with potential medicinal properties. When benzaldehyde compounds react with phenyl hydrazine and ortho-phosphoric acid in an acidic environment, synthesized the indole derivatives. There are two proteins chosen from the protein data bank: the crystal structure of VirB8 from Brucella suis (PDB ID: 2BHM) for evaluation of antibacterial potential and a type of manganese superoxide dismutase (MnSOD) from cyanobacteria (PDB ID: 1GV3) for antioxidant potential. The molecule 3g had the best docking score of -10.4 with the crystal structure of VirB8 from Brucella suis (PDB ID 2BHM), which means it has a strong ability to bind to the target receptor. The molecule 3j had the best docking score of -10.4 against manganese superoxide dismutase (MnSOD) in the membrane of cyanobacteria (PDB ID: 1GV3), showing that it strongly attaches to the target receptor. The result suggests that it has a strong affinity for binding to the target receptor. This result indicates that it has a strong affinity for the target receptor. The metronidazole is taken as a standard for comparison purposes and has a molecular docking score of -6.5 against the crystal structure of VirB8 from Brucella suis. Ascorbic acid is used as a standard. It has a molecular docking score of -5.7 against manganese superoxide dismutase (MnSOD), which is attached to the membrane of cyanobacteria. The remaining design molecules demonstrated excellent docking scores for both proteins when compared to the standard molecules, metronidazole and ascorbic acid. In this study, ProtTox II Software was used to predict the in silico toxicity of all the design molecules. It was found that molecules 3l and metronidazole were respiratory toxic, while compounds 3g, 3k, 3p, and 3r were neurotoxic. All designed molecules were screened for the drug-likeness property and obeyed the criteria of the Lipinski rule of five.

Anaemia is a medical condition characterized by a decrease in the number of red blood cells and the amount of haemoglobin in the blood leading to inadequate oxygen delivery to tissues and organs. Telfairia occidentalis is a dietary leafy vegetable with phytochemicals that are beneficial to human health. This study investigated the phytochemical constituents and hematological effects of the aqueous stem extract of T. occidentalis in a phenylhydrazine (PHZ)-induced hemolytic anemia model in rats. Sixteen male adults Wistar rats were divided into four groups: Normal control group, Negative Control group (PHZ-induced anemic), and two treatment groups receiving PHZ + stem extract at (100 and 200 mg/kg) respectively. Anemia was induced via intraperitoneal injection of PHZ (40 mg/kg) for two days and subsequently the treatment groups administered the aqueous stem extract orally for 14 days. Phytochemical screening was conducted using standard qualitative methods. At the expiration of the study, blood samples were taken from the animals for evaluation of hematological parameters (RBC, WBC, Hb, PCV, MCH, MCHC) and body weight were assessed. Phytochemical analysis revealed the presence of alkaloids, terpenoids, flavonoids, steroids, glycosides, and saponins. Anthraquinones were absent. The anemic control group showed a significant reduction (p < 0.05) in RBC, Hb, and PCV. Treatment with the stem extract, particularly at 200 mg/kg, caused a highly significant (p < 0.001), dose-dependent amelioration of these parameters, elevating them above normal control levels. A significant increase in WBC count and body weight was also observed in the treatment groups. The aqueous stem extract of Telfairia Occidentalis may possesses significant hematopoietic and growth-promoting properties in PHZ-induced anemic rats, attributed to its rich array of bioactive compounds. This could validate its ethnomedicinal use and suggests its potential as a natural, cost-effective intervention for anemia management.

Background: Mangifera indica (Anacardiaceae), known for its many therapeutic potentialities is used in traditional Ivorian medicine to treat anemia. The aim of this study was to evaluate the effects of methanolic extract from the fruit kernel of Mangifera indica (EMMI) on blood pressure, iron and transaminase levels in rat’s anaemic by phenyl hydrazine (50 mg/kg, per os). Methods: The determination of iron, tannins, polyphenols and total flavonoids in EMMI samples was performed, using specific reagents. Different groups of rats were treated with EMMI (100, 500 and 1000 mg/kg) or Folifer (50 mg/kg) by gavage for 14 days. The determination of hematological and biochemical parameters following the different treatments was carried out, using an URIT 3000 PLUS machine. Results: Phytochemical analyses show that EMMI (10 mg/ml), is rich in polyphenols (38.97±0.04 mg GAE/g), flavonoids (26.83±0.02 mg QE/g), tannins (33.87±0.06 mg TAE/g). and iron (52.91±0.01 mg/100 g). The observed anti-anaemic effects were dose-dependent. After 14 days of treatment, EMMI (1000 mg/kg) induced normalization of RBC, Hb, Hct and serum iron levels (p<0.001), with a decrease in ASAT and ALAT levels respectively of about 28 and 34% in anaemic rats (p<0.001). These effects were comparable to those of Folifer (p>0.5). Conclusion: Altogether, this study showed that EMMI normalizes blood count, serum iron and transaminases in rats made anaemic by administration of phenyl hydrazine. This beneficial effect would be attributed to its richness in secondary metabolites and iron.

The development of novel anti-cancer agents remains a critical area of research in the fight against cancer. In this study, a series of pyrazole-clubbed pyrimidine conjugates (5-amino-1-phenyl-3-(4-(pyrimidin-5-yl)phenyl)-1H-pyrazole-4-carbonitriles, (4a–4j) were synthesized via a one-pot reaction of 4-(pyrimidin-5-yl)benzaldehyde, various phenyl hydrazine, and malononitrile in ethanol, under mild conditions using sodium ascorbate as a reducing agent. The synthesized compounds were evaluated for their anti-cancer activity in MCF-7 cell line using docking studies on the EGFR tyrosine kinase domain and MTT assays. The compounds displayed promising cytotoxic effects with IC50 values ranging from 3.56 ± 1.01 µM (for 4i) to 18.01 ± 0.70 µM (for 4c). Notably, compound 4a and 4i, featuring phenyl hydrazine and 2-fluoro phenyl hydrazine, exhibited an IC50 of 4.10 ± 0.26 µM and 3.56 ± 1.01 µM, comparable to the standard anti-cancer drug Sunitinib (IC50: 4.01 ± 0.04 µM). The docking scores of the compounds ranged from −8.6 to −9.9, indicating strong binding affinity with cancer-related molecular targets. DFT calculations and ADMET analyses identified compounds 4a and 4i as promising candidates due to their favorable molecular stability, electronic properties, and excellent pharmacokinetic and toxicity profiles. The results suggest that these pyrazole-pyrimidine conjugates hold significant potential as novel anti-cancer agents, especially in MCF-7 cell-line.

The synthesis of pyridazinone derivatives has gained increasing attention due to their diverse biological activities, particularly as anticancer agents. In this study, novel pyridazinone derivatives substituted with methoxy groups and phenyl hydrazine were synthesized through a multi-step reaction pathway, starting from methoxyacetophenone and glyoxylic acid, followed by cyclization and substitution reactions to yield the target compound 7-(2-methoxyphenyl)-2H-pyridazino[6,1-c][1,2,4]triazine-3(4H)-one. The synthesized compounds were characterized by melting point, TLC, HPLC, UV-Vis, FTIR, NMR, and MS analyses, confirming the expected structures. In silico evaluation was performed using molecular docking against estrogen receptor α (ERα) kinase domain (PDB ID: 1T46), a key protein in breast cancer progression. The docking results showed that the synthesized compounds exhibited strong binding affinities, with compound 8 displaying a binding free energy of –9.1971 kcal/mol and stable interactions with residues Cys673, Leu799, and Phe811. These values were superior compared to the natural ligand and comparable to the reference drug doxorubicin, indicating significant anticancer potential. The results suggest that structural modification of pyridazinone with methoxy and phenyl hydrazine substituents enhances its cytotoxic activity, making it a promising candidate for further development as an anticancer agent.

Diphenylthiocarbazide (DPTC) is a sulfur- and nitrogen-containing organic ligand that holds significant potential as a selective analytical reagent for metal ion detection. In this study, DPTC was synthesized through a condensation reaction between phenyl isothiocyanate and hydrazine hydrate under controlled conditions. The synthesized compound was purified and structurally characterized using UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance (1H NMR), elemental analysis (CHNS), and melting point determination. The presence of key functional groups including thiocarbonyl and aromatic amines confirmed the molecular integrity of DPTC. Spectral studies indicated that DPTC exhibits strong absorbance in the visible range, making it a suitable chromogenic reagent for colorimetric complexation with transition metal ions. Preliminary investigations demonstrated its high affinity and selectivity for copper(II) ions, forming a stable colored complex under mildly acidic to neutral pH conditions. The observed λmax and molar absorptivity values suggest that DPTC can serve as an effective ligand for spectrophotometric detection of trace metal ions. The results pave the way for further application of DPTC in environmental monitoring, pharmaceutical assays, and industrial quality control settings.

Introduction: Approximately 25% of the global population experiences anemia, with preschool-aged children accounting for nearly 50% of these instances. Cuttlefish (Sepia sp.) is a mollusk species used as a food ingredient due to its high nutritional value and potential nutraceutical effects. The present study examines the hematopoietic effect of cuttlefish ink in anemic rats. Methods: Anemia was induced by administering phenylhydrazine (PHZ) intraperitoneally at a dose of 40 mg/kg for two consecutive days. Rats were then divided into three groups (n = 6 each): control, anemia, and Sepia ink treatment (200 mg/kg orally for six days). Results: Sepia ink contains many phenolic and flavonoid compounds. Administration of Sepia ink resulted in a significant improvement in hematological parameters, liver enzymes, serum proteins, iron concentration, kidney biomarkers, antioxidant system, and DNA integrity. Histopathological examination revealed a marked restoration of liver and kidney architecture following Sepia ink administration. Additionally, the Prussian blue stain demonstrated inhibition of hemosiderin expression in liver and kidney tissues. Moreover, immunohistochemistry revealed reduced TNF-α expression in the liver and kidney following Sepia ink treatment. Conclusion: The hematopoietic mechanisms of Sepia ink encompass the enhancement of iron metabolism, stimulation of the antioxidant system, suppression of inflammation, and inhibition of apoptosis.

Anemia a global health issue affects both developed and developing nations, which is characterized by low levels of haemoglobin in the blood and is linked with hepatic damages. Medicinal plants are rich sources of phytonutrients, which play a dominant role in alleviating toxicity linked to vascular toxicity and hepatic and renal impairment. The study aimed to ascertain the impact of crude aqueous extract of Justicia carnea leave (ALJC) extract on liver enzymes and histoarchitectural changes of the liver in phenylhydrazine-induced anemic female wistar rats. Thirty-(30) female Wistar rats weighing 130-150g were used in the study and were divided into five groups of six animals each. Group A received feed and water ad libitum, Group B received 0.1ml of Phenyl-hydrazine (PHZ) only, and group C received 500mg/kg of ALJC for 8-weeks. Group D, E, and F received 1ml of PHZ for two weeks and treated with 250 mg/kg and 500 mg/kg of ALJC and 20mg /kg of Vitamin B12 respectively for 6-weeks through oral gavage. Data obtained for liver functions and bilirubin levels were analysed using SPSS version 25 using ANOVA followed with post Hoc LSD. Body weight was analysed using T-test and values were considered significant at P≤0.05. The result shows ALT, AST, and ALP levels showed a significant increase following PHZ only, while administration of ALJC revealed significant decline in treated groups compared to group A. Total protein had a significant increase and bilirubin indicated a significant decline following PHZ only intake, while treatments with the ALJC and Vitamin B12 showed an improved levels of total protein and bilirubin levels. The PHZ only had a deleterious impact on liver architecture revealing infiltration of hepatocytes, treatments with ALJC and vitamin B12 indicate improved levels of hepatic tissue. The study concludes that ALJC and Vitamin B12 improved hepatic functions and architectural changes, while PHZ only caused an impaired hepatic function.

The study included the preparation and characterization of new compounds called thiazolidine-4-carboximaide in three main steps. In the first step, compound (R) was reacted from the reaction of cysteine ​​with different aromatic aldehydes. Then the compound (R)was reacted with acetic anhydride to prepare the compound (RA). The compound (RA) was reacted with the aromatic amine (phenyl hydrazine) and DCC,HOBT to obtain different amides-4-thiazolidine compounds. All compounds were diagnostic using organic description techniques, FT-IR,magnetic resonance spectroscopy, 1HNMR, and 13CNMR, mass spectroscopy, every the prepared Compounds gave perfect returns. and biological effect prepared compounds:]ML1 ,ML2, ,ML4,ML7 ML8,ML9 [ was studied against cancer cells for two types of cancer lines: human breast cancer line (MCF7) cells, women’s cervical cancer line (Hela), The biological effectiveness of the prepared compoundsML1,ML2, ML4 ML7,ML8 and ML9, was studied against cancer cells for two types of cancer lines, a Michigan cancer foundation (MCF7) and a female cervix carcinoma (Hela). The results of the inspection showed that the compounds ] ML1,ML2, ML4,ML8 ,ML9 [ had high efficacy against a mankind breast cancer cell line and a cell line. Cervical cancer and compound] ML7[ showed little activity on the two types of cancer cell lines. and calculated Inhibition percentage (% ) of prepared compounds effects a Michigan cancer foundation (MCF7) and a female cervix carcinoma (Hela).

ABSTRACT: The right path of investigation in the present study, an efficient and cost-effective method for the preparation of derivatives of 5-Amino-1, 3-diaryl-1 H-pyrazole-4-carbonitriles using Phenyl hydrazine, substituted aromatic aldehyde, and malononitrile in acetonitrile as a solvent employing Mg(NO3)2 as a catalyst under solvent condition. The chemical structures of the titled compounds were confirmed by 1H-NMR &13CNMR, Mass spectral and Elemental analysis. Antimicrobial activities of the titled compounds were also examined by various strains and exhibited mild to moderate anti-bacterial activities. KEYWORDS: Aromatic aldehyde, Phenyl hydrazine, malononitrile, Amino Pyrazole, Mg(NO3)2 , anti-bacterial activities

The haematinic activity of an orally administered ethanolic extract of Bauhinia purpurea leaves was studied on haemolytic anaemic rats. Anaemia was induced by oral administration of phenyl hydrazine for a period of 14 days. red blood cell count, haemoglobin concentration, and packed cell volume were analysed as indices of anaemia. The mean cell haemoglobin, mean cell volume and mean cell haemoglobin concentration were calculated accordingly. Phenyl hydrazine induced a significant decrease (p<0.05) in the blood indicating anaemia and also resulted to significant increase(p<0.05) in the mean cell haemoglobin, mean cell volume values, which are indicator of macrocytosis. leaf extract of Bauhinia purpurea induced a significant (p<0.05) increase in the red blood cell count, haemoglobin concentration, and packed cell volume which has been originally decreased by phenyl hydrazine administration 14 days of treatment. The presence of macrocytosis turn towards normal as the animal recovered from anaemic condition. The results obtained suggested that bauhinia purpurea leaves have hematinic activity

Abstract: In present study, the synthesis of pyrazoline derivatives by the reaction of chalcones with phenyl hydrazine hydrochlorides in ethanol under reflux conditions in good yields.All the prepared compound were characterized by spectral studies. Keywords: pyrazoline, chalcone, phenyl hydrazine, spectral analysis