Primordial black holes (PBHs) and the violation of the null energy condition (NEC) have significant implications for our understanding of the very early Universe. We present a novel approach to generate PBHs via the NEC violation in a single-field inflationary scenario. In our scenario, the Universe transitions from a first slow-roll inflation stage with a Hubble parameter H=H_{inf1} to a second slow-roll inflation stage with H=H_{inf2}≫H_{inf1}, passing through an intermediate stage of NEC violation. The NEC violation naturally enhances the primordial scalar power spectrum at a certain wavelength, leading to the production of PBHs with masses and abundances of observational interest. We also investigate the phenomenological signatures of scalar-induced gravitational waves resulting from the enhanced density perturbations. Our work highlights the potential of utilizing a combination of PBHs, scalar-induced gravitational waves, and primordial gravitational waves as a valuable probe for studying NEC violation during inflation, opening up new avenues for exploring the early Universe.
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