The interest in and significance of herbarium collections for the study of plant biodiversity and evolution has significantly expanded in recent decades due to the introduction of new technologies for large‐scale, automated digitization and the availability of new techniques for DNA sequencing. These innovative methods gave rise to new initiatives with the goal of compiling a sizable molecular and phenological data set. In light of the various national projects that are currently underway and driving the study of herbarium specimens for the purpose of understanding biodiversity loss and habitat shifts as a result of climate change and habitat destruction due to human activity. Here, we showcased a number of cutting‐edge research, mini‐reviews, and technical comments that demonstrate the ever‐growing range of applications for herbarium specimens. It examines the variety of applications for which herbarium specimens are employed, the primary users' profiles of herbarium collections, patterns and systematics of biodiversity, and the evolution of lineages and traits.