Phellinus Pini Research Articles

Chemical composition and bioactivities of Phellinus pini extracts, and quality evaluation of healthy drinks prepared from the mushroom

Chemical composition and bioactivities of Phellinus pini extracts, and quality evaluation of healthy drinks prepared from the mushroom

Selected Species of the Genus Phellinus - Chemical Composition, Biological Activity, and Medicinal Applications.

This study presents the current knowledge on chemical composition, biological activity, and possible medicinal applications of Phellinus igniarius, Phellinus pini, Phellinus pomaceus, and Phellinus robustus. These inedible arboreal species are phytopathogens that cause the enzymatic decomposition of wood. These species belong to the medicinal mushrooms and have been known for centuries in the traditional medicine of the Far East. They have been used as an effective remedy for stomach and intestinal ailments, diarrhea, and hemorrhages. Mycochemical studies have proved the presence of polysaccharides, phenolic compounds, and terpenoids. These compounds show biological activities such as anticancer, antioxidant, antiangiogenic, and antiviral. Research studies conducted using modern analytical methods have advanced the knowledge on the potential therapeutic use of compounds isolated not only from the fruiting bodies but also from biomass obtained with in vitro biotechnological methods.

Aromatic Cadinane Sesquiterpenoids from the Fruiting Bodies of Phellinus pini Block SARS-CoV-2 Spike-ACE2 Interaction.

The ongoing COVID-19 global pandemic caused by SARS-CoV-2 inspires the development of effective inhibitors to block the SARS-CoV-2 spike–ACE2 interaction. A chemical investigation on the fruiting bodies of Phellinus pini led to the isolation of five aromatic cadinane sesquiterpenoids including four new ones, named piniterpenoids A–D (1–4), as well as three known lignans. Their structures were determined by extensive spectroscopic analysis including HRMS and 1D and 2D NMR. All of the aromatic cadinane sesquiterpenoids inhibited the SARS-CoV-2 spike–ACE2 interaction, with IC50 values ranging from 64.5 to 99.1 μM. A molecular docking study showed the disruption of the interaction of compound 1 via hydrogen interactions with Arg403, Asp405, and Arg408 of SARS-CoV-2 RBD and Arg393 and His34 residues of ACE2. These results suggested that aromatic cadinane sesquiterpenoids might be useful in developing agents for COVID-19.

Purified antioxidant from the medicinal mushroom Phellinus pini protects rat H9c2 cell against H2 O2 -induced oxidative stress.

In this study, through the combination of AB-8 macroporous resin, Sephadex LH-20 column chromatography and semi-preparative HPLC, an antioxidant component was purified from the crude extract of Phellinus pini, thereby evaluating the cardioprotective effect of the fraction. As a result, total phenolic content of the 60% ethanol elution was increased by 4.8-fold after one run treatment on Sephadex LH-20 chromatography with gradient elution. After semi-preparative HPLC separation, the first peak (PP-S4-1) showed that inhibition ratio of erythrocyte hemolysis was 91.9%, and inhibition ratio of lipid peroxidation was also increased by 87.6%, at 50μg/ml (p<.01). Based on the results of ESI-MS, 1 HNMR, 13 CNMR, and RP-HPLC compared to many published results, PP-S4-1was identified as catechin (MW 290.015, C15 H14 O6 ). The results showed that PP-S4-1 pretreatment made cell viability increased, and the generation of reactive oxygen species (ROS) inhibited. Meanwhile, PP-S4-1 remarkably decreased the fluorescence intensity of Ca2+ , and increased mitochondrial membrane potential (MMP; ΔΨm). In addition, PP-S4-1 could significantly inhibit the decrease of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activity as well as the increase of MDA content in H9c2 cells induced by H2 O2 . Moreover, pretreatment with PP-S4-1 significantly improved the morphological changes and prevented H2 O2 -induced DNA damage. Therefore, this study clarifies the ability of PP-S4-1 to treat H9c2 cell oxidative stress damage induced by H2 O2 through its antioxidant effect. PRACTICAL APPLICATIONS: This research is not only helpful to elaborate the cardioprotective effect of Phellinus pini but also can contribute to the development of health foods or drug supplements for heart disease in the future. This is the first report dealing with phenolic component and cardioprotective activity of a medicinal mushroom P. pini belonging to the genus Phellinus.

Separation, characterization and hypoglycemic activity in vitro evaluation of a low molecular weight heteropolysaccharide from the fruiting body of Phellinus pini

Edible mushrooms have potential in anti-diabetic phytotherapy. They are rich in natural compounds such as polysaccharides, which have been known to have antihyperlipidemic effects since ancient times. A polysaccharide fraction of PP80 and a contained low molecular-weight (Mw), water-soluble polysaccharide (PPW-1, Mw: 3.2 kDa) were isolated from the fruiting body of Phellinus pini. Both PP80 and PPW-1 possess α-glucosidase inhibition and glucose consumption amelioration in an insulin-resistant HepG2 cell model. The α-glucosidase inhibitory activity of PPW-1 (IC50 = 2.2 ± 0.1 mg mL-1) is significantly (P < 0.01) higher than those of PP80 (IC50 = 13.1 ± 0.5 mg mL-1) and acarbose (IC50 = 4.3 ± 0.2 mg mL-1), behaving in a non-competitive inhibition manner. The structural characterization results indicated that PPW-1 is a homogeneous heteropolysaccharide composed of d-glucose, d-mannose, d-galactose and l-rhamnose. The major backbone of PPW-1 is primarily comprised of 1,6-linked glucopyranose, every third residue of which is branched at the O-3 position by a side chain consisting of 1,3-linked and terminal glucopyranose. In addition, small amounts of 1,2-linked-α-d-Manp, 1,6-linked-3-O-Me-α-d-Galp and rhamnose exist in PPW-1. In summary, PPW-1 is a novel heteropolysaccharide with potent in vitro hypoglycemic activity, and it may be a potential dietary component for improving glucose homeostasis.

СОСТОЯНИЕ ВНУТРИГОРОДСКИХ ЕСТЕСТВЕННЫХ НАСАЖДЕНИЙ СОСНЫ PINUS SYLVESTRIS L. В ЕКАТЕРИНБУРГЕ

Проведен анализ и дана оценка состояния сосновых насаждений, произрастающихвовнутригородской средег. Екатеринбурга в условиях ежегодно нарастающего антропогенного воздействия. Сложившаяся ситуация приводитк повышениюрискападения деревьев иопасностипотери уникальных городских сосновых насаждений. Объект исследования – внутригородское чистоесосновое насаждение площадью около 4 га. Установлено, что снижение устойчивостисосновых древостоев на городской территории являетсяследствием воздействия комбинации рядафакторов: изменение гидрологическогорежима лесного участка, механические повреждения деревьев пристроительстве, поражение стволовой гнилью. С увеличением класса возраста деревьев процентгнили как на высотествола 1,3 м, так и укорневойшейки возрастает, спреобладанием площадипоражения на высоте 1,3 м. Абсолютные значения годичного радиальногоприроста не зависят отповреждения грибомсосновая губка Phellinus pini (Thore et Fr.) Pil. Установленастатистическизначимаясвязь между инфицированиемрастений и высокочастотными характеристиками радиального прироста. Насегодняшний день в городском законодательственет четко разработанных диагностических шкал для оценки на ранних стадиях аварийности сосновых деревьев, в том числе со скрытой гнилью, что приводит к необходимости акцентировать внимание на данной проблеме. Результаты исследований могут быть учтены при принятии управленческих решений по уходу, оценке и прогнозированию состояния фрагментарных внутригородских сосновых насажденийв урбоэкосистемах Екатеринбурга.

EVALUATION OF HEARTROT CAUSED PHELLINUS PINI AND RELATED YIELD LOSS IN PINUS SYLVESTRIS STANDS

Tree wounds are the starting points that may lead to heartwood discoloration and decay caused by invading micro-organisms such as heartrot caused Phellinus pini. Phellinus pini most frequently occurs on douglas–fir (Pseudotsuga menziesii), pine (Pinus sylvestris) and spruce trees (Picea abies). According to investigations made in this area, infection by Phellinus pini entries through felling scars or broken tops when Phellinus pini fruiting bodies (conks) on other trees are realising airbone spores. When spores land on a freshly wounded stem, the infection process starts. Internal decay is often difficult to detect because only Phellinus pini conks indicate its presence. In most cases the number of conks doesn’t exceed one-two pieces on the surface of inficied Pinus sylvestris stems. When wood poles for power lines are harvested in length from 10 to 16m in wood felling areas where the average height of Pinus sylvestris trees is more than 27m, the local distribution of heartrot doesn’t exceed for more than 1.5m above and below each conk, the conk is located in the middle part of the pole length, the speeed of pruning exceeds 4m/sek, the hight level of risk exists that the damaged with heart rot wood pole will be accepted as appropriate to quality requirements toward heartrot because after pruning the presence of conk would be vanished but the top and butt surfaces of pole’s won’t indicate heartrot. The evidence of heartrot will be checked only in the technological process of debarking before impregnation when Phellinus pini conk place indicates. The purpose of this study is to predict the influence of Phellinus pini on Pinus sylvestris stems and to provide operational and safety recommendations concerning the risky assessment and management of infected felling areas in harvest planning processes.

Hyperlipidemic Inhibitory Effects of Phellinus pini in Rats Fed with a High Fat and Cholesterol Diet

This study evaluated the in vitro and in vivo hypolipidemic effects of the medicinal mushroom Phellinus pini. The methanol extract (ME) of the fruiting body of Ph. pini was active against pancreatic lipase and cholesterol esterase with 99.14% and 67.23% inhibited activity at 1.0 mg/mL, respectively. It also inhibited 81.81% and 55.33% of α-glucosidase and α-amylase activities, respectively, at 2.0 mg/mL. Hyperlipidemia as induced by feeding rats with a high fat and cholesterol diet (HFC). HFC supplemented with a 5% fruiting body powder of Ph. pini (HFC + PhP) significantly reduced plasma total cholesterol, low-density lipoprotein cholesterol, and triglycerides in rats compared with HFC. The reduced levels were comparable to rats fed the normal control diet (NC). The atherogenic index of HFC + PhP rats was significantly lower than that of the HFC rats. The excretion of fecal total lipid and cholesterol in the HFC + PhP rats was significantly higher than those in the NC and HFC rats. Histopathological examinations demonstrated scant deposition of lipids in the liver of rats fed HFC + PhP. The dietary supplementation with the fruiting body powder provided natural plasma lipid and glucose lowering effects in experimental rats without adverse effects on the plasma biochemical parameters and liver function related enzyme activities. Therefore, the hypolipidemic effects of Ph. pini may be due to the inhibitory effects on pancreatic lipase, cholesterol esterase, α-glucosidase, and α-amylase, and excretion of excess lipids and cholesterol in the feces.

THE SPREAD OF DISEASES IN VOLYN REGION FORESTS

Some peculiarities of expansion characteristics and general dynamics of the forest disease cores in Volyn region were studied in Volyn Regional Forestry and Hunting Industry Administration (VRFHIA). The dynamics of sanitary selective and solid felling with area distributions connected with VRFHIA in 2016 was illustrated. The main problems and tendencies of forest sanitary conditions were explained. The area of lesions the most dangerous forest diseases for 2016 if found to increase by 954.8 ha or 3.8 %. Having analysed Volyn region forest sanitary condition the authors have ascertained that generally 1220.5 ha of disease cores were eliminated by means of forest protection activities and written off during the report year. The biggest size is the concentration of Root fungus (Heterobasidion annosum (Fr.) Bref. 1889) and it remained the same as in the previous years. It covers 13961 ha or in other words it is 55 % of general surface of disease cores in this region. 5117 hectares need some methods of felling. Root fungus (Heterobasidion annosum (Fr.) Bref. 1889) has developed rapidly because of the long lasting drought season and the ground water level decrease. During 2016 Volyn RFHIA enterprises tried to solve the problem by solid sanitary felling in the areas of 332 ha with disease concentration. This is 2.4 % of the general land area of these nucleuses. The most common forest diseases are defined to be as follows: stem rot (Fomes fomentarius (L) Fr.1849) – 2363 hа; aspen fungus (Phellinus tremulae (Bondartsev & P. N. Borisov 1953) – 993 hа; diametrical Oak cancer (Pseudomonas quercus Schern.) – 922 hа; oak fungus (Daedalea quercina (L.) Pers.1801) – 676 hа; birch polyporus (Piptoporus betulinus (Bull.) P. Karst.1881) – 657 hа; red rot (Phellinus pini) – 562 hа; pitch streak (Cronartium flaccidum (Alb. & Schwein.) G. Winter 1880) – 369 ha; alder fungus (Phellinus igniarius (L.) Quel. 1886) – 313 ha; bacterial ash cancer (Pseudomonas savastanoi (Janse 1982) Gardan, et al., 1992) – 216 ha; much smaller (Armillariella mellea (Vahl) P. Kumm. 1871) – 183 hа. In order to protect and preserve forests when forestry proceedings, a complex of sanitary and recreational events and sanitary requirements are taking place.

Preliminary Investigation on the Chemical Constituents and Antioxidant Activities of Two &amp;lt;i&amp;gt;Phellinus&amp;lt;/i&amp;gt; Mushrooms Collected in Foshan

The Phellinus mushrooms have been known for its immunomodulatory, hypolipidemic, and anticancer activities. In the current work, the chemical constituents of two Phellinus mushrooms, Phellinus pini 141016# and Phellinus sp. 150802#, which were collected in Foshan city, Guangdong province, were investigated preliminarily by pre-test tube method and by comparison with the known natural compounds from Phellinus pini, and their antioxidant activities were evaluated by the biochemical assay of hydroxyl and 2,2-diphenyl-1-pricylhydrazyl (DPPH) radicals scavenging activity in vitro. The results show that both the two Phellinus mushrooms contain phenolics, alkaloids, lactones, steroids and terpenoids, and have potent antioxidant activities. The IC50 values of DPPH radicals and hydroxyl radicals for Phellinus pini 141016# were 74.37 μg/mL and 59.69 μg/mL, respectively, and the IC50 values of DPPH radicals and hydroxyl radicals for Phellinus sp. 150802# were 98.95 μg/mL and 165.47 μg/mL, respectively. They are comparable to the antioxidant activity of the standard antioxidant ascorbic acid.

Structural properities and immunoenhancement of an exopolysaccharide produced by Phellinus pini

Phellinus pini exopolysaccharide (PPE) is a mannan produced by P. pini when cultured in potato dextroseagar medium. Partial hydrolysis with acid, periodate oxidation and Smith degradation, methylation and 13C nuclear magnetic resonance (NMR) spectroscopy were used to elaborate its structure. Results indicated that PPE contained a backbone of (1→2)-linked Man, which was heavily substituted via (1→6)-glucosidic bonds with (1→3)-linked Man, and terminated mainly with Man, as well as a small amount of Gal and Glc. Immunological tests showed that PPE exhibited high macrophages-activating ability, thereby augmenting phagocytosis and enhancing the production of nitric oxide, tumour necrosis factor-α and reactive oxygen species. Therefor, PPE had potential applications in developing functional food or in serving as a natural immnunostimulant.

Habitat indicators for cavity-nesters: The polypore Phellinus pini in pine forests

Foresters and arborists have long used fruit-bodies of heart-rot fungi as signs of advanced live-tree decay, but such usage has not been elaborated for forest conservation. I analysed relationships between a heart-rot fungus, tree-cavity supply, and cavity-nesting bird assemblage in wet hemiboreal Scots pine (Pinus sylvestris) forests in Estonia. The focal species, Phellinus pini, is the main heartwood decayer of live pines; it typically forms fruit-bodies at the stage of advanced decay on old trees. I found that the pine wetlands had few tree-cavities (mostly in snags) and cavity-nesting birds (woodpeckers being almost absent) despite abundant snag supply. Only one fruit-body of P. pini was found on cavity-tree but stand-scale abundance of the fruit-bodies correlated well with cavity-nester densities. Multifactor models indicated that cavity formation, not tree death, was the limiting process for secondary cavity-nesters, and P. pini could indeed be used (in combination with old-pine abundance) for assessing their habitat quality. This fungus could also serve as an educational flagship species to bridge conservation biology and forest pathology, and its fruit-bodies can signal trees to be retained at harvesting in pine forests. The conclusion is that there is hope for developing practical indicators to manage for the hidden decay processes that govern tree-cavity development.

Phenolic compounds in Scots pine heartwood: are kelo trees a unique woody substrate?

Deadwood quality can be a highly significant factor in determining the occurrence of deadwood-dependent organisms such as saproxylic fungi. Rare deadwood substrates that are produced only after a lengthy senescence such as kelo trees may have unique deadwood qualities. Using high-performance liquid chromatography, we compared the phenolic composition of six types of Scots pine (Pinus sylvestris L.) substrates: living mature trees with no fungal sporocarps, living mature trees with Phellinus pini sporocarps, fallen non-kelo trees, soon-to-be kelo (standing), standing kelo, and fallen kelo. The fungal-infected living trees and fallen kelos were found to have more similarities in their phenolic composition when compared with the living and fallen trees and the standing kelos, which gets further pronounced with increasing decay. The results also highlight the uniqueness of the fungal-infected living trees and the fallen kelos and illustrate a possible correlation between fungal infection and the heartwood phenolic composition of Scots pine. However, it remains unclear to what extent the differences in phenolic compositions are caused by fungal infection and fungal decomposition. We also observed a previously undocumented correlation between the phenolic groups and fire scars on the trunks of the trees. The variation in substrate quality warrants further consideration when deadwood restoration activities are planned, as the quality of the deadwood could be as equally important as the quantity.

Evaluation of Anticholinesterase and Inflammation Inhibitory Activity of Medicinal Mushroom Phellinus pini (Basidiomycetes) Fruiting Bodies.

Phellinus pini, a medicinal mushroom, has been used as folk medicine in Asian countries for treating ailments such as cancer and gastrointestinal diseases. In this study we evaluated in vitro the antidementia and anti-inflammatory activities of Ph. Pini fruiting bodies. Eleven phenol compounds were detected by high-performance liquid chromatography analysis. Acetylcholinesterase and butyrylcholinesterase inhibitory effects of a methanol extract and a hot water extract were moderate and comparable with those of galanthamine, the standard drug used to treat the early stages of Alzheimer's disease. The methanol extract had a neuroprotective effect against glutamate-induced cytotoxicity on PC-12 cells at concentration ranging from 20 to 40 µg/mL. The mushroom extracts also inhibited the production of nitric oxide and the expression of inducible nitric oxide synthase in lipopolysaccharide-induced RAW 264.7 macrophages, and they significantly inhibited in vivo carrageenan-induced hind-paw edema in rats. Therefore, it is suggested that Ph. Pini fruiting bodies possess anticholinesterase and anti-inflammatory effects.

Stimulation of the activity of a novel tannase produced in white-rot fungi Phellinus pini, Fomes fomentarius, and Tyromyces pubescens by medium supplementation.

In recent years, tannase has gained increasing interest mainly because of its potential applications. One of the most important functions of tannic acid (TA) hydrolase is the release of gallic acid (GA) from complex tannins. The aim of the study was to determine the dynamic changes in tannase activity depending on the carbon source in the culture medium. An extracellular and intracellular tannase activity analysis was carried out with the use of spectrophotometric analysis and confirmed by capillary electrophoresis in cultures of white-rot fungi: Phellinus pini, Fomes fomentarius, and Tyromyces pubescens. The inducible potential of TA and rapeseed meal on the activity of tannin acyl hydrolase was confirmed during 14 days of culturing. Different effects of the tested compounds on stimulation of tannase activity in selected fungal strains have been demonstrated. We concluded that rapeseed meal was the best inducer of tannase activity in the case of P. pini. However, the highest concentrations of GA were observed after stimulation by the TA in the cultures of F. fomentarius and T. pubescens.

Chemical Characterization and In Vitro Antioxidant Activity Evaluation of Polysaccharides from the Fruiting Bodies of the Red Heart Mushroom Phellinus pini (Higher Basidiomycetes).

Phellinus pini is a precious medicinal mushroom. Three water-soluble fractions of crude polysaccharides (PP30, PP60, and PP80) were obtained from the fruiting bodies of Ph. Pini. The basic chemical characterization and in vitro antioxidant activity of these 3 polysaccharides were determined. All 3 crude polysaccharides were heteropolysaccharide complexes with a small amount of protein (1.14-2.55%) and uronic acid (2.06-4.11%). The monosaccharide composition of PP30, PP60, and PP80, as a molar ratio, was mannose (1.00):glucose (18.7):galactose (0.92), fucose (0.47):3-0-Me-Gal (0.51):mannose (1.00):glucose (7.86):galactose (1.10), and rhamnose (0.12):fucose (0.32):xylose (0.17):3-0-Me-Gal (0.26):mannose (1.00):glucose (4.79):galactose (0.53), respectively. The in vitro antioxidant activities of crude polysaccharides were evaluated by 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, hydroxyl radical, and ferric-reducing antioxidant power methods. The antioxidant data obtained using these methods were in accordance with each other and decreased in the same order of PP80 > PP60 > PP30 at a concentration of 0.1-2.5 mg/mL.

A contribution to the distribution and importance of Phellinus pini (Thore ex Fr.) Pil. var. abietis (P. Karst.) Pil. in the Carpathian Range, Czechoslovakia included

A contribution to the distribution and importance of Phellinus pini (Thore ex Fr.) Pil. var. abietis (P. Karst.) Pil. in the Carpathian Range, Czechoslovakia included

Characterization and antioxidant activities of the polysaccharides from mycelium of Phellinus pini and culture medium.

Two polysaccharides isolated and purified from the mycelium (PPM) and its culture medium (PPE) of Phellinus pini using gel filtration were subjected to composition analysis and valuated for the antioxidant activity. The average molecular weights of PPM and PPE were approximately 22.0 and 38.0 kDa, respectively. PPM and PPE were both neutral heteropolysaccharides consisting of mannose, galactose, and glucose with molecular ratios of 2.99:1.00:0.34 and 38.40:1.00:1.76, respectively. In vitro antioxidant assay, PPM and PPE could scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical, chelate ferrous ion and reduce ferric ion. The antioxidant activities of PPM were stronger than those of PPE, suggesting that PPM has significant potential as a natural antioxidant agent.

Effective stimulation of the biotechnological potential of the medicinal white rot fungus: Phellinus pini by menadione-mediated oxidative stress.

The effect of menadione (MQ; 2-methyl-1,4-naphtoquinone), a superoxide-generating agent, on the natural biodegradation system in the medicinal white rot fungus Phellinus pini was determined. While measuring the activities of extracellular manganese-dependent peroxidase (MnP) and intracellular chitinase, it was found that the application of MQ (0.75 mM) distinctly stimulated the activities of these enzymes in comparison to the control values (without MQ). Using the capillary electrophoresis (CE) method, an increase in the extracellular oxalic acid (OXA) concentration was detected during the first days after the addition of MQ. It was observed that the rate of intracellular proteolysis at pH 3.5 evidently decreased under oxidative stress conditions. Contrary to these results, the activities of serine proteases at pH 9.5 measured against fluorogenic peptide substrates distinctly increased in stressed cultures. The MQ treatment also caused an evident increase in the catalase (CAT) activity, as well as the levels of superoxide anion radicals (SORs), formaldehyde (FA), and phenolic compounds (PHC) in the experimental cultures. The results obtained confirm that prooxidants may find application as an effective way to stimulate biotechnological production of MnP and chitinase by white rot fungi.

Effect of heat on interspecific competition in saprotrophic wood fungi

Some boreal wood fungi that are associated with forest fire or open dry habitats have an increased resistance to heat in comparison to species associated with a less specific distribution or species found in mesic forests. We hypothesize that extreme temperature-stress experienced during fires will favor species adapted to heat and, ultimately, the composition of species inhabiting logs in such habitats will change. Competitiveness after temperature stress was examined in three fire-associated species – Dichomitus squalens , Gloeophyllum sepiarium and Phlebiopsis gigantea – and three non fire-associated species – Ischnoderma benzoinum , Phellinus pini and Fomitopsis pinicola . There was a difference between the fire-associated species and the non fire-associated species with respect to competitive strength after heat stress. All fire-associated species had an advantage after heat treatment, colonizing a larger volume of wood than any non-fire-associated competitor. Our findings suggest that increased heat tolerance of mycelia can exert a competitive balance shift after forest fire. It shows that a system governed by forest fire will be dominance controlled under certain conditions. Furthermore, from a management perspective, during a prescribed burning, certain species already present in the ecosystem will be favored if the fire is not allowed to totally consume the substrates. • Wood fungi was subjected to heat and tested in combative interactions. • Temperature was calibrated to match the conditions during a forest fire. • Wood fungi associated with forest fire gains competetiveness after heat treatment. • All interactions resulted in a gain of substrata for fire associated wood fungi. • Forest fire can change the intercompetetive balance, favouring adapted species.