Phayao Province Research Articles

Factors influencing the organizational engagement of village health volunteers, after the COVID-19 Outbreak in Phayao Province

Village health volunteers (VHVs) play a crucial role in promoting health advancements and enhancing community well-being. This cross-sectional descriptive study explores the factors that influence organizational engagement among VHVs in Phayao Province. Data were gathered from 400 volunteers using Krejcie and Morgan’s formula and analyzed through a questionnaire. Analytical methods included descriptive statistics, Pearson’s product-moment correlation, and multiple linear regression. The findings revealed that the majority of volunteers were female (77%), aged between 41-60 years (62.3%), married (81.3%), with over ten years of experience (61%), and had educational levels of high school or vocational certificate (46.8%). Most were agriculturists (63.5%), with incomes below 5,000 baht (54%), and served as general members (74.5%) in a single role (78.8%), primarily under the provincial administrative organization (54%). The scores for motivational factors, quality of life, and organizational engagement were high. Responsibility, the nature of work, and respect were identified as significant predictors of organizational engagement, accounting for 51.1% of the variance. These findings were statistically significant (R = 0.715, R Square = 0.511, P-value < 0.001). Based on these results, organizations are encouraged to ensure appropriate working conditions and respect to enhance engagement among village health volunteers.

Comprehensive breast cancer risk analysis with whole exome sequencing and the prevalence of BRCA1 and ABCG2 mutations and oncogenic HPV.

Breast cancer is the most prevalent cancer and also the leading cause of cancer death in women worldwide. A comprehensive understanding of breast cancer risk factors and their incidences is useful information for breast cancer prevention and control planning. The present study aimed to provide information on single nucleotide polymorphisms (SNPs) and copy number variations (CNVs) in breast cancer, the allele frequency of two SNPs in breast cancer-related genes BRCA1 DNA repair associated (BRCA1; rs799917) and ATP binding cassette subfamily G member 2 (ABCG2; rs2231142), and the prevalence of human papillomavirus (HPV) infections in a normal population living in Phayao Province, Northern Thailand. One breast cancer and 10 healthy samples were investigated by whole exome sequencing (WES) and compared for genetic variation. The WES data contained SNPs in genes previously implicated in breast cancer and provided data on CNVs. The allele frequencies for SNPs rs799917 and rs2231142 were also examined. The SNP genotype frequencies were 35.88% CC, 46.54% CT, and 17.58% TT for rs799917 and 33.20% CC, 46.88% CA, and 19.92% AA for rs2231142. A total of 825 human whole blood samples were examined for HPV infection by PCR, and the pooled DNA was tested for HPV infection using metagenomic sequencing. No HPV infections were detected among all 825 samples or the pooled blood samples. The incidence of breast cancer among the tested samples was estimated based on acceptable breast cancer risk factors and demographic data and was 1.47%. The present study provided data on SNPs and CNVs in breast cancer-related genes. The associations between SNPs rs2231142 and rs799917 and breast cancer should be further investigated in a case-control study since heterozygous and homozygous variants are more common. Based on the detection of HPV infection in the blood samples, HPV may not be associated with breast cancer, at least in the Northern Thai population.

Extending the wisdom of Tai Lue weaving to create the values of handcraft and traditional wisdom for community furniture at Wat Yuan, Thailand

This work aims to study consumer demand for newly designed Tai Lue fabric patterns for decorating bar stools; to investigate consumer demand for the materials, colour, and design requirements of bar stools; and to produce prototype bar stools decorated by Tai Lue weaves based on customer preferences. Data were collected from interviews and a literature review was conducted to design a bar stool that incorporates Tai Lue weaving. A questionnaire was distributed to better understand the desires of customers, such as newly designed patterns, materials, colours, and design requirements of bar stools. Then a prototype bar stool was produced and customer preferences and satisfaction were identified and analysed. It was found that the most popular Tai Lue woven pattern is the Dok Sor pattern mixed with the Saeng pattern. This is suitable for producing cushions and backrests because it is tightly woven, flexible and adds to the furniture's appealing feeling. Furthermore, a prototype was produced using a D-style chair made with wood of a natural colour, which is simple and can be produced in the community. This prototype bar stool is suitable for use in coffee shops. Customer satisfaction was measured in terms of its Tai Lue weaving appreciation, acceptance, ease of use, comfort, satisfaction, and durability. The customer demand scored the highest at 4,000 baht (110 USD). Finally, this study can encourage the use of traditional Tai Lue weaving patterns, thereby creating additional value and income for the Tai Lue community at Wat Yuan, in Chiang Khan district, Phayao province, Thailand.

Fish diversity in the Nong Leng Sai Wetland, Phayao Province, Northern Thailand, after deep-water dredging

Abstract. Tuncharoen S, Manetam K, Pornpuang S, Chaisan W, Panprommin D, Soonthornprasit K, Pitakpol S. 2024. Fish diversity in the Nong Leng Sai Wetland, Phayao Province, Northern Thailand, after deep-water dredging. Biodiversitas 25: 2738-2746. This study aimed to explore changes in the diversity of fish species in the Nong Leng Sai Wetland, Mae Chai District, Phayao Province, Northern Thailand, after just completed deep-water dredging while shallow-water dredging is in progress. Therefore, three different gill net mesh sizes collected fish specimens from January to July 2022. The results showed that 25 species belong to 23 genera, 13 families from 7 orders, and 21 native and 4 introduced fish species. The fish comprised of the miscellaneous group was the most composed by weight and number, accounting for 45.5% and 52%, respectively, followed by the carp, 38.6% by weight and 45.6% by number. The fish diversity after dredging has decreased, including species and quantity, compared to the diversity reported before. Physical changes in the Nong Leng Sai Wetland resulted from deep water dredging, including increasing water depth, more water storage volume, increased turbidity, and lack of fish shelter and natural food, affecting the diversity of fish species. Furthermore, dredging has stimulated the recovery of aquatic ecosystems regarding water quality, habitat of aquatic animals, and appropriate natural food, which is important for increasing the diversity of fish species and quantities.

Eastern Lanna Product Design for Guest room of Northern Hotel in Thailand

This research is integrated the architecture and art design creative work with community for developing local products of Eastern Lanna, by design the interior decoration prototype in 36 square meter guest room (price 600 - 800 baht per night) of Phayao university hotel to inform Kham Mok Luang flower as symbolic media or one element of identity. The objectives of study is to set research teamwork of interior designer, architect, artist- designer to design decorative objects or props with community craftmans to increase value added handicraft products and community can develop their products to commercial business by their competency. The method is qualitative research that 4 designers collaborate with 10 textile and basketry handicrafts in Ang Thong district, Phayao Province by sharing their knowledges and skills together, start from design the symbolic pattern of Kham Mok Luang of cloth weaving and basketry raw material process, design the guest room decoration, produce the decorative objects or props : curtain, bed runner, head of bed, lamp, basket. After finishing the props the researcher set them in the mock up room, and take the pictures of decorative room and objects to be survey tool of stakeholders satisfaction opinion about symbolic pattern design and all decorative products. The result is to the most satisfaction in symbolic pattern, the products or props satisfaction is more to the most.

Marketing Communication Through Brand Storytelling of The Communitique Products in Phayao Province

Marketing Communication Through Brand Storytelling of The Communitique Products in Phayao Province

Assessment of Nonprescription Medicine and First Aid Knowledge Among School Health Teachers in Northern Thailand

This research aimed to assess nonprescription medicine knowledge, first aid knowledge, and compared the knowledge scores with and without previous training among school health teachers in Phayao Province, Northern Thailand. This cross-sectional study included school health teachers from primary and secondary schools by using the quota and purposive sampling methods. The data were collected using a self-administered questionnaire with three sections: socio-demographic characteristics, nonprescription medicine assessment, and first aid assessment. The data were gathered and analyzed using descriptive and independent sample t-test analysis. A total of 152 school health teachers participated in this study, with a response rate of 94.4%. The mean age was 41.5 ± 10.6 years, and 92.8% of the teachers were females. The majority (97.4%) of the teachers did not have a degree related to health sciences. 73.0% of the teachers had no previous nonprescription medicine training, while 59.2% had no previous first aid training. Only 15.8% of teachers were knowledgeable in nonprescription medicine, and only a few (0.7%) were knowledgeable in first aid. In addition, higher knowledge scores of nonprescription medicines (P-value < 0.001, 95% CI = 1.71 – 3.69) and first aid (P-value < 0.001, 95% CI = 2.83 – 4.19) were statistically significant among the teachers who had received previous training. This highlights the importance of training teachers about the use of nonprescription medicines and first aid management so they can respond appropriately to first aid needs in schools and could be incorporated into a support policy and integrated into the teachers’ training curriculum.

Determinants of nonprescription medicine and first-aid knowledge among primary school health teachers in Phayao Province, Northern Thailand

Determinants of nonprescription medicine and first-aid knowledge among primary school health teachers in Phayao Province, Northern Thailand

A randomized controlled clinical trial examining the effects of Cordyceps militaris beverage on the immune response in healthy adults

Cordyceps militaris (L.) Link (C. militaris) contains various beneficial substances, including polysaccharides (galactomannan), nucleotides (adenosine and cordycepin), cordycepic acid, amino acids, and sterols (ergosterol and beta-sitosterol). It also contains other essential nutrients, such as protein, vitamins (E, K, B1, B2, and B12), and minerals (potassium, sodium, calcium, magnesium, iron, zinc, and selenium). Due to the numerous health benefits of supplements and products containing C. militaris extract, their popularity has increased. However, the immunostimulant effect of C. militaris remains unclear. Therefore, this study developed a functional beverage from the submerged fermentation of C. militaris (FCM) and aimed to investigate the potential of FCM in healthy male and female volunteers in Phayao Province, Thailand. This study provides essential information for the development of healthy drink products. Healthy men and women were provided either FCM containing 2.85 mg of cordycepin or placebo for 8 weeks (n = 10 for each gender). The immune cell markers, immunoglobulins, and safety parameters were assessed initially at baseline and at 4 and 8 weeks. The NK cell activity markedly increased in the male FCM group from baseline (p = 0.049) to 4 weeks after receiving FCM. Compared with those in the placebo group, the NK activity in women who received FCM for 8 weeks significantly increased (p = 0.023) from baseline. Within-group analysis revealed that the IL-1β levels were markedly reduced in the male FCM group (p = 0.049). Furthermore, the IL-6 levels decreased from baseline in the female FCM group (p = 0.047). The blood sugar, lipid, and safety indices were not different between the experimental groups. FCM can potentially be developed as an immune-boosting supplement without liver, kidney, or blood component toxicity.

Factors influencing the Co-production in water management policies: A multi-case study in Nan province and Phayao province, Thailand

The issue of sustainable development has garnered significant international attention, with the United Nations (UN) playing a central role in promoting this agenda through the establishment of the Sustainable Development Goals (SDGs). The objectives of this study were twofold. Firstly, it aimed to investigate the factors that influence co-production and assess their impacts on sustainability. Secondly, this study aimed to provide suggestions for policymakers and implementers on how to enhance co-production factors to achieve greater sustainability outcomes. The research focused on two case studies in Thailand: water management in Ban Tun sub-district, Mueang district in Phayao province, and water management in Rong Ngae village in Worranakorn sub-district, Pua district in Nan province. A qualitative research approach was employed, utilizing in-depth interviews, non-participatory observations, and focus group discussions. Triangulation was employed to ensure data validity, and semi-structured interviews were used as the data collection tool. Content analysis and qualitative data analysis software were employed for data analysis. The findings revealed co-production in both the formulation and implementation of water management policies relating to sustainable outcomes. These outcomes were influenced by societal norms, cultural factors, existing policies, and knowledge, which shaped the relationships among the communities, stakeholders, and agencies involved in water management. The factors affecting co-production and its sustainability in the multi-case study's sustainable development policy were identified as follows: 1) contextual factors, including societal standards, culture, and existing policies and knowledge; 2) inputs, comprising water management knowledge and collaboration involving resources, technology application, and legal regulations; and 3) outputs, encompassing the expansion of knowledge and the development of competencies. The study emphasizes the need to boost knowledge expansion and competency through collaborative knowledge-sharing and academic involvement. It suggests that promoting inclusive participation and equal collaboration among stakeholders enhances co-production and sustainable outcomes.

Effects of a community-based participatory educational program on knowledge, attitude, and practice for preventing work-related musculoskeletal disorders risks among rural workers: A quasi-experimental study

Work-related musculoskeletal disorders (WMSDs) have become a paramount concern in occupational health, significantly affecting the well-being and efficiency of laborers across diverse sectors. This research aims to investigate the effects of a community-based participatory educational program on enhancing knowledge, attitude, and practice (KAP) for preventing WMSD risks among rural workers in Phayao province, Thailand. A single-group quasi-experimental design was employed, with assessments conducted at the baseline (T1), post-program (T2), and follow-up post-program (T3) stages. The participants attended an eight-week educational program. Data collection tools included demographic characteristics, assessment of KAP related to WMSDs, and evaluation of WMSD risks. The data were analyzed using descriptive statistics and repeated-measures analysis of variance. In total, 90 participants were included in the study, with an average age of 47.6 ± 10.6 years. More than two-thirds of the participants were classified as general workers. The results indicated a significant increase in knowledge scores, rising from 6.78 ± 1.59 at baseline to 9.56 ± 0.70 post-program and maintaining at 8.59 ± 1.06 after follow-up (p-value < 0.001, η2 = 0.681). Attitude scores also demonstrated a significant improvement, increasing from 36.43 ± 6.92 to 46.66 ± 1.94 post-program and remaining at 45.97 ± 2.05 after follow-up (p-value < 0.001, η2 = 0.674). Practice scores witnessed a substantial increase from 19.31 ± 6.49 at baseline to 38.54 ± 1.18 post-program, maintaining at 37.79 ± 1.34 after follow-up (p-value < 0.001, η2 = 0.881). Furthermore, the results indicated a notable decrease in WMSD risks post-program intervention, sustained after follow-up. The study suggests that integrating a community-based educational program successfully enhanced participants’ KAP related to WMSD risks. These findings provide valuable insights to the expanding body of evidence, offering pertinent information for policymakers.

Management of cultural tourism in Ban Toon, Muang District, Phayao Province, Thailand during the COVID-19 pandemic.

The research aims to study the process of managing cultural tourism in the community and assess the socio-economic impact on the community with the help of eight key contributors to the project who play an essential role in the implementation of cultural tourism management. We prepared field notes from interviews and observations, and provided details from data collection (in the form of transcripts). Thematic analysis was employed to answer the research questions using a content analysis tool to obtain the community context in cultural tourism and tourism management dimensions. Participatory Action Research (PAR) was employed to meet the needs of stakeholders and test tourism routes through such routes. The tourism route development was evaluated using descriptive statistics. There is one new cultural tourism route and the process of managing cultural tourism developed community-level organizations with spiritual leaders as the main advisors. The result of the net present value (NPV) analysis is 917,149 baht or 27,792$. The return from the implementation of this project (the internal rate of return (IRR)) is 11.32%. In addition, the ratio of the sum of the present value over the life of the project to the sum of the present value of the cost over the life of the project is 2.45 times, giving the project, if continued, an income 2.45 times greater than its expenditure. Cultural tourism management in Ban Toon, Muang District, and Phayao Province during the COVID-19 pandemic can be used as a guideline in other communities with similar contexts.

Morpho-phylogenetic identification and characterization of new causal agents of Fusarium species for postharvest fruit rot disease of muskmelon in northern Thailand and their sensitivity to fungicides.

A significant global problem affecting muskmelon (Cucumis melo L.) is fruit rot caused by phytopathogenic fungi, which results in unsaleable products and substantial financial losses. In 2022 and 2023, fruit rot on muskmelon was found during the postharvest storage period in Phayao Province of northern Thailand. The aim of the current study was to isolate the species of fungi causing the fruit rot lesions. Out of the rot lesions on muskmelons, nine fungal isolates were received. All isolates of fungi were identified through a combination of morphological characteristics and molecular analyses. Based on their morphological traits, all isolated fungal isolate was assigned to the genus Fusarium. All the fungal isolates were determined to belong to the Fusarium incarnatum-equiseti species complex through multi-gene phylogenetic analysis employing the calmodulin (cam), RNA polymerase second largest subunit (rpb2), and translation elongation factor 1-alpha (tef1-α) genes. These isolates were identified as F. compactum (SDBR-CMU483), F. jinanense (SDBR-CMU484, SDBR-CMU485, and SDBR-CMU486), F. mianyangense (SDBR-CMU487 and SDBR-CMU488), and F. sulawesiense (SDBR-CMU489, SDBR-CMU490, and SDBR-CMU491). Moreover, pathogenicity tests were subsequently carried out, and the results indicated that all fungal isolates caused symptoms of fruit rot on inoculated muskmelon fruits. Notably, this result was consistent with the symptoms observed throughout the postharvest storage period. In the fungicide screening test, all fungal isolates showed sensitivity to copper oxychloride. However, all isolates showed insensitivity to benalaxyl-M + mancozeb, carbendazim, mancozeb, and metalaxy. To the best of our knowledge, the present study is the first to identify F. compactum, F. jinanense, and F. mianyangense as new causative agents of muskmelon fruit rot in Thailand and other regions globally. This is also the first report of postharvest fruit rot on muskmelons caused by F. sulawesiense in Thailand. Furthermore, the fungicide screening results indicate that fungicide resistance can be beneficial in developing potential management strategies against postharvest fruit rot disease of muskmelon caused by these four pathogenic Fusarium species.

Prevalence, virulence characteristics, and antimicrobial resistance of Vibrio parahaemolyticus isolates from raw seafood in a province in Northern Thailand.

Vibrio parahaemolyticus (V. parahaemolyticus) is commonly found in seawater and seafood products, but evidence is limited of its presence in seafood marketed in locations very distant from coastal sources. This study determined the prevalence and characterization of V. parahaemolyticus in seafood from markets in landlocked Phayao province, Northern Thailand. Among 120 samples, 26 (21.7%) were positive for V. parahaemolyticus, being highest in shrimp (43.3%), followed by shellfish (36.7%), and squid (6.7%), but was not found in fish. V. parahaemolyticus comprised 33 isolates that were non-pathogenic and non-pandemic. Almost all isolates from shrimp and shellfish samples were positive for T3SS1. Only five isolates (15.2%) showed two antimicrobial resistance patterns, namely, kanamycin-streptomycin (1) carrying sul2 and ampicillin-kanamycin-streptomycin (4) that carried tetA (2), tetA-sul2 (1), as well as one negative. Antimicrobial susceptible V. parahaemolyticus isolates possessing tetA (67.9%) and sul2 (3.5%) were also found. Six isolates positive for integron class 1 and/or class 2 were detected in 4 antimicrobial susceptible and 2 resistant isolates. While pathogenic V. parahaemolyticus was not detected, contamination of antimicrobial resistance V. parahaemolyticus in seafood in locations distant from coastal areas requires ongoing monitoring to improve food safety in the seafood supply chain.

Mechanism of Regulation on the Marketing of the Breast–Milk Substitutes: a Lesson Learn from one Province in Northern of Thailand

Objectives: To investigate the existing model and mechanism for surveillance of the marketing of food for infants and young children in Phayao province, Thailand according to The Control of Marketing of Infants and Young Children Food Act, B.E. 2560 (2017). Method: This research was a qualitative research study which collected data through an in-depth interview and a focus group with 7 informants: officers of Phayao Provincial Public Health Office, registered nurses, public health technical officers, and consumer network officers. Results: Phayao province had a control model and mechanism on the marketing of food for infants and young children according to The Control of Marketing of Infants and Young Children Food Act, B.E. 2560 (2017) as follows: (1) For passive surveillance, there was a complaint center at the Provincial Public Health Office as the main coordinator to receive complaints and prepare files of violation cases. (2) Active surveillance included visits to target places such as shops and child development centers, and visits to enforce maternal and child health standards. Finally, (3) networking and knowledge dissemination were implemented through the first 1,000 miracle days of life project. The implementational model for controlling the marketing of food for infants and young children should be as follows: (1) Be compliant with laws. (2) Determine clear policies at the departmental levels. (3) Work collaboratively at the provincial, district, and subdistrict levels. (4) Increase participation of related people for surveillance of offences. (5) Empower operational networks, entrepreneurs, and other people to gain knowledge and be compliant with the law in a suitable way. (6) Use communication technology to support implementation. Conclusion: Phayao province has a model and mechanism to control the marketing of food for infants and young children in compliance with the law. The model and mechanism should be developed according to the area contexts to support the implementation to be more effective.

Predictive Factors of Stress among the Population in Pong District, Phayao Province, Thailand, after the COVID-19 Outbreak

Predictive Factors of Stress among the Population in Pong District, Phayao Province, Thailand, after the COVID-19 Outbreak

Buckling behavior of Dendrocalamus sericeus Munro bamboo bars: Experiments and application

Bamboo is an alternative natural material that is widely used in engineering applications, especially in terms of sustainable construction. However, there are a few studies on the local bamboo's behavior, especially in northern Thailand. For an in-depth study on this local natural resource, this paper conducts experimental tests on the Dendrocalamus sericeus Munro bamboo (DSMB) bars to investigate the buckling behavior. The tests include the free-free resonance test (FFR), compression test, buckling load test, and microstructure experiments. A total of 108 long buckling specimens were provided, including variations in specimen length, moisture content, and cross-sectional size. There are two types of specimens, namely the dry and soak specimens, that were obtained from the Phayao province in Thailand and are 3–5 years old. From the experimental data, the statistical method is employed to investigate the distribution of these data and establish the modified Euler buckling stress for the prediction of the critical buckling stress of the DSMB bars. Finally, two numerical simulations were provided to validate the proposed model and present the DSMB bar application on the RC column. The analytical results show that the proposed equation yields acceptable results with the experiment at an R2 of 0.9402, while the numerical model shows that if the amount of DSMB bars is adequate, the DSMB bars can provide the equivalent load capacity as the steel bars.

Development and Creation of Ancient Sandstone Carvings Using 3D Software Tools and Mobile/Tablet Devices

In the area of Phayao Province that used to be part of the ancient Lanna Kingdom, there are unique works of art that can be found until today, which are sandstone carvings caused by religious beliefs resulting in works such as Buddha statues. These are art related to religion. Nowadays, these works of art have started to fade away and receive less and less attention from people. The creation of art objects in this research therefore uses the interpretation of the creative objects in line with people’s lifestyles by selecting the lotus, which is a plant related to rivers, and is important in religion. In the lotus, which is interconnected and aligned with Buddhism, and in harmony with a way of life that aligns with the river, creative works are found in various forms, connecting people in Buddhism, including sandstone carving. In this creation, digital technology tools and methods are used to collect data to create a 3D work piece. Photogrammetry is used to record detailed proportions and information and customization of 3D work pieces. In these steps, mobile phone-type tools are used to collect image data to create the 3D work or an application is used to customize 3D work pieces from a tablet device to create prototypes of sandstone sculptures from the artisans that remain today. This can be seen in creating, maintaining, and recording digital data, creation, and the integration of knowledge. This demonstrates that today’s tools and portable devices can help create more creative pieces of work and preserve art and culture.

The Co-Production and Sustainability Policies: Multi- Case Study in Water Management Policies in Thailand

Co-production and sustainability are intertwined concepts, especially in water management. Co-production is a powerful tool for promoting sustainability by fostering collaboration between government representatives and community members to organize public services professionally. This study aimed to 1) comprehensively examine co-production in water management in Nan and Phayao Provinces and 2) investigate co-production models in policy formulation and implementation. It focuses on two case studies: Ban Tun Sub-district in Phayao Province and Rong Ngae Village in Nan Province, Thailand. This study adopted a qualitative research design, utilizing the methods of document analysis, in-depth interviews, and non-participatory observation. Data triangulation was employed to ensure data validity, and a semi-structured interview was utilized as the data collection tool. Content analysis and qualitative data analysis software were used to analyze the data. The findings revealed full co-production, influenced by norms and cultures, existing policies, and knowledge that collectively informed the relationships among the communities, stakeholders, and agencies working in the water management sector. The co-production model combined top-down and bottom-up policymaking, guided by Van Meter & Van Horn’s implementation model, promoting stakeholder collaboration for sustainable water management. Recommendations included 1) expanding knowledge and capacity-building for stakeholders in water management, encompassing existing knowledge bases and new technologies, and 2) developing explicit guidelines and improving communication channels to promote co-production.

Diversity of trematode cercariae among naturally infected lymnaeid snails from Phayao, Thailand.

Lymnaeids are aquatic snails playing an important role in the transmission of many parasitic trematode species of veterinary and medical importance. In this study, we assessed the presence of cercarial flukes in naturally infected lymnaeid snails from Phayao province, Thailand, and determined the species diversity of both the intermediate snail hosts and parasite larvae. A total of 3,185 lymnaeid snails were collected from paddy fields at 31 sites in eight districts of Phayao province between October 2021 and December 2022. Larval fluke infection was assessed using the cercarial shedding method. The collected snails as well as emerging cercariae were identified at the species level via morphological and molecular methods. The sequences of snail internal transcribed spacer region 2 (ITS2) and cercarial 28S ribosomal RNA gene (28S rDNA) and cytochrome C oxidase1 (Cox1) were determined by PCR amplification and sequencing. Three species of lymnaeid snails were detected in this study, including Radix (Lymnaea) rubiginosa (Michelin, 1831), Radix (Lymnaea) swinhoei (Adams, 1866) and Austropeplea viridis (Quoy & Gaimard, 1832), of which R. rubiginosa was the most abundant, followed by A. viridis and R. swinhoei. The overall rate of trematode cercarial infection in the lymnaeid snails was 2.8% (90/3,185); the cercarial infection rate in R. rubiginosa and A. viridis was 3.5% (60/1,735) and 3.1% (30/981), respectively. No larval fluke infection was observed in the studied R. swinhoei (0/469). Nine morphotypes of cercariae were detected at 15 sites from four districts. The emerging cercariae were molecularly identified as Clinostomum sp., Aporocotylidae sp., Apharyngostrigea sp., Trichobilharzia sp., Apatemon sp., Pegosomum sp., Petasiger sp., Echinostoma revolutum and Plagiorchis sp. These findings emphasize the occurrence and diversity of trematode cercariae among naturally infected lymnaeid snails in Phayao province and could contribute to broadening our understanding of the host-parasite relationships between trematodes and their first intermediate hosts as well as developing effective interventions to control trematode parasitic diseases.