Phases Of Wound Healing Process Research Articles

Effectivity of 5% Temulawak Extract (Curcuma xanthorrhiza) on Post-Extraction Fibroblast Cells in Wistar Rats (Rattus norvegicus)

ABSTRACT Introduction: Socket wound would undergo 3 phases of wound-healing process, i.e. inflammation phase, proliferation phase, and re-modelling phase. In proliferation phase, fibroblast cells were important for producing collagen and elastic cells. One of natural ingredients believed to stimulate fibroblasts is Curcuma xanthorriza. The active compounds in curcuma, namely curcumin and flavonoid, had the ability to increase fibroblast migration and accelerate wound healing. This study aims to observe the effectivity of 5% temulawak extract (Curcuma xanthorrhiza) on post-extraction fibroblast cells in Wistar rats (Rattus norvegicus). Methods: The experiment was conducted on 30 rats that were divided into 2 groups, control group and experimental group. Each group of rats were given intermuscular anesthesia and underwent mandibular incisors extraction. The experiment group was given intermuscular injection of 5% curcuma extract into their wound socket, while control group were given placebo gel injection. On day 3, 5, and 7 decapitation was performed to get the mandibular samples. Furthermore, the number of fibroblast cells was observed using a light microscope with 3 different fields of view at 400 times. Results: The results showed that the mean number of fibroblasts in the experiment group was higher than the control group. Conclusion: 5% temulawak extract (Curcuma xanthorrhiza) had significant effect on increasing post-extraction fibroblast cells in Wistar rats (Rattus norvegicus).

Efficacy of Jatropha (Jatropha curcas L.) Sap Cream on Total Differential Leucocytes in Inflammatory Phase of Wound Healing on Mice Skin (Mus musculus) : A Review

A wound is a damage to the integrity of the epithelium of the skin. There are three phases of wound healing process, the first phase is inflammatory phase, the second phase is fibroplasia phase, and the third is maturation phase. Inflammation that occurs during the wound healing process should be limited because it would cause the wound to undergo an abnormal healing process and pathological inflammation if it persists. The aim of this study was to determine the efficacy of jatropha (Jatropha curcas L.) sap cream on total differential leucocytes in inflammatory phase of wound healing on mice skin. The method used was literature study using qualitatSive methods. Data used in this study refers to the result of studies which have been conducted and published in national and international online journal. Result of data analysis showed that Jatropha sap cream was effectively limiting and inhibiting the leucocytes accumulation in inflammatory area and stimulated new blood vessels formation (angiogenesis) in inflammatory phase of wound healing in mice skin. Based on the result of this study, it can be concluded that the Jatropha sap cream could reduce the number of differential leucocytes in inflammatory phase of wound healing on mice skin.

Efficacy of Mentha pulegium essential oil encapsulated into nanostructured lipid carriers as an in vitro antibacterial and infected wound healing agent

Nanostructured lipid carriers are used for protecting essential oils against environmental factors and also for increasing antimicrobial capability. Gels prepared from Mentha pulegium essential oil (MPO)-loaded into nanostructured lipid carriers (MPO-NLCs) might hasten the infected wound healing process. This study evaluates in-vitro antibacterial activity of MPO-NLCs and in-vivo wound healing activity of MPO-NLCs in BALB/c mice model. To evaluate the effect of MPO-NLCs on wound healing, wound contraction, tissue bacterial count, histological and molecular parameters were assessed at 3, 7 and 14 days after creating wound. MPO-NLCs showed the most antibacterial activity against positive bacteria of Staphylococcus epidermidis, Staphylococcus aureus and Listeria monocytogenes and also negative bacteria of Escherichia coli and Pseudomonas aeruginosa (P < 0.01). Topical administration of MPO-NLCs shortened inflammatory phase and improved proliferative phase. Moreover, administration of MPO-NLCs increased the expressions of IL-10, TGF-β and b-FGF and also decreased the levels of NF-κB compared to the control group (P < 0.05). In sum, the MPO-NLCs promoted infected wound healing by increasing antibacterial properties, decreasing inflammatory phase and accelerating proliferation phase of wound healing process. On the basis of the results, MPO-NLCs can be used an agent for the treatment of the infectious wounds.

In vitro evaluation of the therapeutic potential of Anatolian kermes oak (Quercus coccifera L.) as an alternative wound healing agent

The present study was designed to evaluate the wound healing activity of the extracts from Quercus coccifera L. Q. coccifera L. is used in the treatment of burns and wounds in traditional medicine. Although the wound healing activities of various Quercus species have been demonstrated previously, no scientific report has been published on the wound healing potential of Q. coccifera L. The purpose of the present study was to contribute to elucidate the mechanism of the wound healing effect of Q. coccifera L. and to contribute the development of an effective therapeutic agent from this plant. The effects of aqueous (AE) and methanolic (ME) extracts from the stem parts of Q. coccifera L. on different phases of wound healing process were investigated on 3T3-Swiss albino mouse fibroblasts and murine macrophage (RAW 264.7) cell line. Fibroblast proliferation was significantly promoted by AE and ME up to 136.8% and 163.38% at 200 μg/mL concentrations, respectively. Also, our results demonstrated that the rate of wound closure was stimulated by AE (18.6%–52.1%) and ME (23.9%–76.41%). AE and ME showed the highest activities on hydroxyproline synthesis (9.43 and 15.6-fold compared with control) at 200 μg/mL concentration. Both extracts potently inhibited lipopolysaccharide (LPS)-induced production of tumour necrosis factor-alpha (TNF-α) in RAW 264.7 macrophages, at all tested concentrations. Phenolic content of ME (165.88 ± 2.32 mg gallic acid equivalent/g) was higher than AE (67.58 ± 0.91 mg gallic acid equivalent/g), as well as the 2,2-diphenyl-1 picrylhydrazyl (DPPH) assay revealed that ME has more potent antioxidant activity with IC50 value of 58.7 ± 2.42 μg/mL. The minimum inhibitory concentration (MIC) values ranged between 0.25 and 250 μg/mL, against all tested strains. Extracts from Q. coccifera L. have antioxidant and antibacterial potential and accelerated the wound healing process evidenced by enhanced fibroblast proliferation, wound closure rate, collagen synthesis and reduced inflammation. Our results indicated that Q.coccifera L. has immense scope as an effective source to develop a therapeutic agent against skin wounds. The results of this study also have provided scientific basis for the traditional use of Q.coccifera L. as a wound healing agent.

Natural dietary antioxidants containing flavonoids modulate keratinocytes physiology: In vitro tri-culture models

Ethnopharmacological relevanceSeveral traditional medicinal herbs are widely used for dermatologic and cosmetic preparations. The beneficial skin repair activity is detected in various phases of wound-healing process, such as cell-cell, cell-matrix interactions or collagen synthesis.Aim Of The Study: The study assessed the effects of Opuntia ficus-indica (L.) Mill. (Opuntia) and Milk Thistle (MT) (Silybum marianum (L.) Gaerth) on adult keratinocytes (HaCaT) functioning under basal condition or in the presence of mechanical damage (wounded cells). Materials and methodsThe role of the natural compounds was tested on HaCaT grown in mono-culture and tri-culture configurations. In tri-cultures models, HaCaT were treated with the conditioned media (CM) obtained by Human Normal Dermal Fibroblast (NHDF) and Human Dermal Microvascular Endothelial cells (HMVEC) co-cultures. Specifically, were tested cell viability, oxidative stress mechanisms (cytokines release and lipid peroxidation) and cellular remodelling (growth factors release or metalloproteinase modulation). Moreover, the migratory potential of HaCaT was analysed by the use of wound healing in vitro assay. ResultsOpuntia and MT differently modified the metabolism (EGF, MMP-9), and the migratory properties of HaCaT both under physiological conditions or upon mechanical damage (wounded cells). Moreover, both compounds modulated HaCaT response to oxidative stress. The response to the natural compounds were modified, and in some cases potentiated, in tri-culture configuration systems. ConclusionsThe data demonstrated that in vitro tri-culture approach is suitable to characterize the role of natural compounds on the complex communication between dermal-epidermal cellular components and microvascular endothelium. Specifically, Opuntia and MT are good alternatives to synthetic compounds in skin repair promotion.

Effects of cannabidiol, a Cannabis sativa constituent, on oral wound healing process in rats: Clinical and histological evaluation.

The effects of cannabidiol (CBD), the main non-psychotropic component of Cannabis sativa, were assessed on oral wound healing in an in vivo model. Standardized ulcers were induced in 60 Wistar rats using a 5-mm biopsy punch on the midline of the ventral tongue. Animals received intraperitoneal injections of CBD at doses of 0 (control), 5, and 10mg/kg daily. Animals were weighed daily, and wound healing was clinically and histologically evaluated after 3 and 7days of treatment. CBD treatment did not influence the wound area of ulcerative lesions at either observation time. Conversely, microscopic findings revealed that at Day 3 postwounding, CBD-treated lesions exhibited significantly lower inflammatory scores than those in the control group. However, this difference was not observed at Day 7. Collectively, these findings indicate that CBD exert an antiinflammatory effect in early phase of wound healing process although it was not sufficient promote clinical improvement of oral traumatic ulcerative lesions.

Invalid freeze-dried platelet gel promotes wound healing

Wound healing is the curative process of tissue injury, composed of three phases: the inflammatory phase, proliferative phase, followed by the maturation cum remodeling phase. Various treatment options were previously depicted for wound healing, however a treatment that accelerates these phases would be highly valuable. Platelet aggregation at the bleeding vessels and release of various growth factors are the most promising factors that stimulates the wound healing progress. In the present study, we hypothesized that the freeze-dried platelet which were normally discarded from the blood banks due to invalidity, might be promising to accelerate the phases of wound healing. The invalid freeze-dried platelets were prepared to a gel form called invalid freeze-dried platelet gel (IF-PG), which was tested for its efficacy in a cutaneous punch wound model in rats. Mupirocin antibiotic gel was used as a bio-equivalent formulation. The wound healing phases and changes in the wound sites were determined by assessing the wound sizes, histopathological analysis, immunohistochemical staining. The re-epithelialization at the wound sites at different time intervals till the wound closure was also determined. Our results suggest the beneficial effects of IF-PG; in reducing the wound area and accelerating wound closure in the cutaneous punch wound in rats. Histopathology and immunostaining results support the improvements in the wound when treated with IF-PG, which were similar to that of mupirocin antibiotic gel. Our preliminary findings also warrant the competency of IF-PG in modulating the different phases of wound healing process. In conclusion, IF-PG might be a resourceful alternative for the wound care management, however further studies are required to validate its impact on various growth factors before proceeding to clinical studies.

ДОСЛІДЖЕННЯ РАНОЗАГОЮВАЛЬНОЇ ДІЇ МАЗІ З ЕКСТРАКТОМ СОСНИ ЗВИЧАЙНОЇ

&lt;p align="center"&gt;&lt;strong&gt;RESEARCH OF &lt;/strong&gt;&lt;strong&gt;THE WOUND &lt;/strong&gt;&lt;strong&gt;HEALING&lt;/strong&gt;&lt;strong&gt; ACTION OF THE&lt;/strong&gt;&lt;strong&gt; OINTMENT WITH PINE EXTRACT&lt;/strong&gt;&lt;strong&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p align="center"&gt;&lt;strong&gt;&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;strong&gt; &lt;/strong&gt;&lt;strong&gt;T&lt;/strong&gt;&lt;strong&gt;. &lt;/strong&gt;&lt;strong&gt;P&lt;/strong&gt;&lt;strong&gt;. &lt;/strong&gt;&lt;strong&gt;Mandzii&lt;/strong&gt;&lt;strong&gt;, &lt;sup&gt;2&lt;/sup&gt; А. &lt;/strong&gt;&lt;strong&gt;R&lt;/strong&gt;&lt;strong&gt;. &lt;/strong&gt;&lt;strong&gt;Grytsyk&lt;/strong&gt;&lt;strong&gt;, &lt;sup&gt;3&lt;/sup&gt; А. О. Kl&lt;/strong&gt;&lt;strong&gt;y&lt;/strong&gt;&lt;strong&gt;menko, &lt;sup&gt;4&lt;/sup&gt; І. О. Nyektyehayev&lt;/strong&gt;&lt;/p&gt;&lt;p align="center"&gt;&lt;sup&gt;1,2,3&lt;/sup&gt; SHEI "Ivano - Frankivsk national medical university", Ivano – Frankivsk&lt;/p&gt;&lt;p align="center"&gt;&lt;sup&gt;4&lt;/sup&gt; SHEI "Lviv national medical university named after Danylo Galyts’kiy",&lt;/p&gt;&lt;p align="center"&gt;Lviv&lt;/p&gt;&lt;p align="center"&gt; &lt;/p&gt;&lt;p&gt;&lt;strong&gt;Introduction&lt;/strong&gt;&lt;strong&gt;. &lt;/strong&gt;The range of medicines intended for the local conservative treatment of wounds is growing steadily. Currently, there is no universal drug suitable for application in all phases of wound healing process: purulent-necrotic, phases of regeneration (the formation of granulation tissue) and scarring, because each phase of treatment requires a differentiated selection of drugs with different mechanisms of action.&lt;/p&gt;&lt;p&gt;Worked out literature sources have shown the prospect of pine dosage form development. &lt;em&gt;Pinaceae &lt;/em&gt;family consists of 10 genera and around 250 species and is the most common group among coniferous class and among gymnosperms in general. &lt;em&gt;Pinus&lt;/em&gt; genus includes the greatest number of species. It contains about 100 species represented by evergreen trees. The most widespread in Ukraine is pine (&lt;em&gt;Pinus s&lt;/em&gt;&lt;em&gt;y&lt;/em&gt;&lt;em&gt;lvestris&lt;/em&gt; L.).&lt;/p&gt;&lt;p&gt;In medical practice pine buds (&lt;em&gt;Gemmae Pini&lt;/em&gt;, or &lt;em&gt;Turiones pini&lt;/em&gt;) and products derived from pine, such as turpentine (&lt;em&gt;Oleum Therebintyinae&lt;/em&gt;), essential oil (&lt;em&gt;Oleum Pini sylvestris&lt;/em&gt;), rosin&lt;em&gt; &lt;/em&gt;(&lt;em&gt;Pix liquida Pini&lt;/em&gt;) and activated charcoal&lt;em&gt; &lt;/em&gt;(&lt;em&gt;Carbo activata&lt;/em&gt;) are used.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Investigation&lt;/strong&gt;&lt;strong&gt; &lt;/strong&gt;&lt;strong&gt;methods&lt;/strong&gt;&lt;strong&gt;.&lt;/strong&gt; Wound healing activity was studied in experiments &lt;em&gt;in vivo&lt;/em&gt; on 32 white outbred rats according to the "General ethical principles of animal experimentation" (Ukraine, 2001). Experiment was conducted on animals weighing 170 -192 g, who were on a general diet and kept in the same conditions. To reproduct the model of linear wound rats were introduced into barbamil anesthesia (0,8 ml of 1 % aqueous solution at barbami for 100 mg of body weight). After their hair was cut off, a piece of skin sized 1×1 cm was carved in aseptic conditions (operating area was moistened with 5% alcoholic solution of iodine).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results and discussion&lt;/strong&gt;&lt;strong&gt;.&lt;/strong&gt;&lt;strong&gt; &lt;/strong&gt;The obtained results indicate that the acceleration of wound healing process was observed under the application of the ointment with extract of pine (reduction of wound area to 50,10 ± 3,34 and 2,13 ± 0,52 mm&lt;sup&gt;2&lt;/sup&gt; on the 7&lt;sup&gt;th&lt;/sup&gt; and 14&lt;sup&gt;th&lt;/sup&gt; day respectively). Thus, ointment with extract of pine as the medicine for the treatment of wounds in the experiment prevails the referent medicine (group 4) and provides wound healing in a shorter time. Under visual observation of group number 2 the change in wound area on the 7&lt;sup&gt;th&lt;/sup&gt; and 14&lt;sup&gt;th&lt;/sup&gt; day (50,10 and 2,13 mm&lt;sup&gt;2&lt;/sup&gt; respectively) was observed.&lt;/p&gt;&lt;p&gt;The development of wound process was accompanied by changes in protein metabolism and level of middle mass molecules (total protein of animals of the control group and the group number 2 was 45,6 ± 1,17 g/l and 57,4 ± 2,9 g/l respectively, the content of middle mass molecules was 0,29 ± 0,02 standard units and 0,22 ± 0,01 standard units respectively).&lt;/p&gt;&lt;p class="aCxSpFirst"&gt;&lt;strong&gt;Conclusions&lt;/strong&gt;&lt;strong&gt;. &lt;/strong&gt;&lt;/p&gt;&lt;ol&gt;&lt;li&gt;The results of conducted researches show that the application of an ointment with pine extract for the treatment of wound process provides wound healing at a shorter time.&lt;/li&gt;&lt;li&gt;Reparative activity of the ointment with pine extract applied to the wound area shows more pronounced activity in comparison with the referent medicine.&lt;/li&gt;&lt;/ol&gt;&lt;p class="aCxSpMiddle"&gt;&lt;strong&gt;References&lt;/strong&gt;&lt;strong&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p class="aCxSpMiddle"&gt;1. Shalymov O. O. Suchasne medykamentozne likuvannya ran / O. O. Shalymov, V. F. Sayenko, B. M. Datsenko // Vidomcha instruktsiya.– K., 2002.– 37 s.&lt;/p&gt;&lt;p class="aCxSpMiddle"&gt;2. Martin P. Inflammatory cells during wound repair: the good, the bad and the ugly / P. Martin, S. J. Leibovich // Trends Cell Biol.– 2005.– V. 15, № 4.– R. 599–607.&lt;/p&gt;&lt;p class="aCxSpMiddle"&gt;3. Velnar T. The wound healing process: an overview of the cellular and molecular mechanisms / T. Velnar, T. Bailey, V. Smrkolj // J. Int. Med. Res.– 2009.– V. 37, № 5.– R. 1528–1542.&lt;/p&gt;&lt;p class="aCxSpMiddle"&gt;4. Zast·souvannya roslyn klasu khvoyni u medytsyni. Rodyna Sosnovi (Ohlyad literatury) – T. M. Kozymenko, L. H. Dudchenko, T. YU. Hrabova [ta inshi] - Fitoterapiya. Chasopys – №2 – K.: 2014 – S. 34 – 39.&lt;/p&gt;&lt;p class="aCxSpMiddle"&gt;5. Ranozazhyvlyayushchaya aktyvnostʹ mazy, soderzhashchey éfyrnoe maslo travy tysyachelystnyka poymennoho / S. D. Trzhetsynskyy, V. Y. Mozulʹ, H. A. Zhernov [y dr.] – Naukovo - praktychnyy zhurnal – Zaporizhzhya : Aktualʹni pytannya farmatsevtychnoyi i medychnoyi nauky ta praktyky, № 2 (15) – 2014. – S. 50 – 53.&lt;/p&gt;&lt;p class="aCxSpMiddle"&gt;6. Doklinichni doslidzhennya likarsʹkykh zasobiv: Metodychni rekomendatsiyi / Za red. chl.-kor. AMN Ukrayiny O. V. Stefanova. – K.: Avitsena, 2001. – 528 s.&lt;/p&gt;&lt;p class="aCxSpMiddle"&gt;7. Teoryya y praktyka mestnoho lechenyya hnoynykh ran (problemy lekarstvennoy terapyy) / Pod red. B. M. Datsenko. – K.: Zdorov'ya, 1995. – 383 s.&lt;/p&gt;&lt;p class="aCxSpMiddle"&gt;8. Rany y ranevaya ynfektsyya: Rukovodstvo dlya vrachey / Pod red. M. Y. Kuzyna, B. M. Kostyuchonok. – 2-e yzd. – M.: Medytsyna, 1990. – 592 s.&lt;/p&gt;&lt;p class="aCxSpLast"&gt;9. Kamyshnykov V. S. Spravochnyk po klynyko - byokhymycheskoy laboratornoy dyahnostyke: V 2 t. – Mn.: Belarusʹ, 2000. – T. 1. – 495 s.&lt;/p&gt;

EFFECT OF NOVEL OINTMENT COMPOSITION AG/TAGA: CHITOSAN-GEL ON HEALING OF EXPERIMENTAL WOUNDS WITH DIFFERENT ORIGINS

The purpose of the study – evaluation of the regenerating activity of two-component paste, consisting of silver containing finely particulate sorbent: chitosan gel. Material and methodologies. For the study we used novel excipient, consisting of two components: silver containing finely particulate sorbent: chitosan gel in ratio one-to-one. Novel sorbent agent, taken for study, consists of thermally activated silica containing aluminum hydroxide in the form of its modification silver. Experiment was conducted on 48 Wistar models in case of scalp (degloving) wound (abscission of skin area 30–40 mm2 in size on depilated side surface) and chemical burn (applying 1–2 drops of 70% sulfuric acid on the depilated surface side. Regenerating activity was evaluated based on morphometry area of the wound surface, fixing the rate of scarring. Evaluation was made histologically on 4th - micrometers paraffin sections, stained with hematoxylin and eosin, and semithin sections, stained with toluidine blue. The results of study. Composition (paste) developed from silver containing finely particulate sorbent: chitosan gel sped up (for 3 days) the process of reparative regeneration of tissue on the model of chemical burns, helped to structuring formations in skin by the type of granuloma, at the same time there were no signs of perifocal inflammation. In case of scalp wound ointment composition wasn’t conducive to raising the rate of regeneration. Conclusion . Developed excipient (paste) is effective at the first phase of wound healing process, which was showed on scalped and burn wounds, as hydrophilic base had a large sorption capacity. Traumatic effect of dry sorbent powder (powder) on the surface of the wound was offset by connecting the sorption component and a hydrophilic ointment base.

Reparation effects of vacuum wound therapy in patients with diabetic foot syndrome.

Vacuum Therapy (Vacuum-assisted closure, VAC) - a method of complex therapy which is used to improve the healing of both acute chronic wounds in patients with diabetic foot syndrome. Due to widespread introduction of this technique, unique cell, extracellular and general effects of its use in the treatment of patients with purulent-necrotic complications of diabetic foot syndrome, a technique of vacuum wound therapy has been successfully used in many surgical departments of Ukraine. Despite the diversity of the clinical effects of VAC-therapy and appearance of number of publications, in the domestic and foreign literature physiological basis of this method have not been studied completely. The effectiveness of vacuum therapy explain: the reduction of the size of the wound, the stabilization of the wound environment (due to the removal of inflammatory mediators and cytokines), microdeformation and remodeling of cell, rows reduction of edema, reduction of bacterial contamination, etc. At the same time, mechanisms of blood flow enhancement at different duration of VAC-therapy use, remain unclear its effect on the third phase of wound healing process as well.

Hyperglycemia is associated with lower levels of urokinase-type plasminogen activator and urokinase-type plasminogen activator receptor in wound fluid

AimsWounds in patients with hyperglycemia show impaired healing. Plasminogen activation is crucial in several overlapping phases of wound healing process. In this study, we aimed i) to compare acute wound fluid in patients with hyperglycemia and normoglycemia, ii) to focus on the elements of plasminogen activation in the wound fluid, and iii) to determine if the acute wound fluid characteristics are associated with surgical site infections. MethodsIn a cohort of 54 patients, a closed suction drain was placed in the wound above the anterior abdominal wall fascia under the skin in order to collect postoperative acute wound fluid samples for 3 following days after colorectal surgery. Patients were classified as normoglycemic (n=25) or hyperglycemic (n=29; 17 with type 2 diabetes and 12 with stress induced hyperglycemia). Surgical site infection was defined according to the Centers for Disease Control criteria. The levels of urokinase-type plasminogen activator (uPA), urokinase-type plasminogen activator receptor (uPAr), plasminogen activator inhibitor-1 (PAI-1), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and fibroblast growth factor-1 (FGF-1) were measured in the wound fluid. ResultsCompared to normoglycemic subjects, patients with hyperglycemia had significantly lower levels of uPA and uPAr in the wound fluid despite similar or even higher circulating levels. There was no significant difference in IL-1β, TNF-α, PAI-1 and FGF-1 levels. In the whole study population, the wound fluid levels of uPA and uPAr were negatively correlated with circulating glucose levels. No difference was detected in the wound fluid characteristics of patients with and without surgical site infection. ConclusionPatients with hyperglycemia exhibit decreased levels of uPA and uPAr in the wound fluid, suggesting a local failure in plasminogen activation at the wound site.

Modern approaches to local treatment of pyo-necrotic complications of the diabetic foot syndrome

The analysis of publications devoted to modern methods of treatment of pyo-necrotic complications of diabetic foot syndrome has been presented. Identified were the most important principles of therapeutic tactics for this type of pathology. In order to create optimal conditions for wound healing after surgical sanitation local drug treatment is carried out - a thorough wound detersion with application of the isotonic sodium chloride solution or a neutral bathing solution, periodic (as needed) necrectomies, application of bandages, in accordance with the phase of wound healing process. The choice of medication for local treatment depends on the one hand on the type of lesion of the lower limbs, on the other - on the diabetes related morbidity and phase of wound healing. In the first phase of wound healing antiseptics are used: iodophors, polyhexanide (lavasept), hydroxymethyl quinoxaline dioxide (dioxidine), and in the absence of ischemia - compositions based on gelevine, water-soluble base ointments. In the second phase used were various wound covers based on collagen, as well as oil and hydrogel dressings. Ready-to-use bandages are also used, which are multi-layered system, comprised of a cellulose substrate, collagen, fibroblasts, growth factor, coated with plates of silicon to control humidity. The disadvantage of the ready-to-use dressings is the impossibility of controlling the wound on a daily basis. One of the trends in the treatment of chronic diabetic wounds is the use of living skin cells that serve as a source of growth factors, cytokines and other proteins that stimulate the healing process. The use of local immunomodulators is seen as promising. Treatment should be individualized and systemic antibiotic therapy is required. The main principle of local treatment is the creation of optimal conditions for accelerating the healing process.

The wound healing properties of Channa striatus-cetrimide cream-wound contraction and glycosaminoglycan measurement

Haruan has been proved to influence the different phases of wound healing process. The current research focuses on the effects of haruan on the different constituents of extracellular matrix of healing wounds in normal and diabetic rats. Anaesthetized normal and streptozotocin induced diabetic rats were provided with excision wounds at the back and then animals were divided into four groups as: group 1, wounds treated with cetrimide+haruan cream; group 2, wounds treated with haruan cream; group 3, wounds treated with cetrimide (commercial) cream; and group 4, wounds untreated and served as control. Animals were sacrificed after 3, 6, 9 and 12 days. These wounds were used to determine the hexosamine, protein, uronic acid and glycosaminoglycan contents and the wound contraction. The results suggested a marked increase ( P<0.05) in the uronic acid, hexosamine and dermatan sulfate contents on day 3 of group 1 when compared with groups 2–4. Wound contraction of group 1 was also markedly enhanced of group 1 ( P<0.01) when compared with groups 2– 4. On the basis of these results, we conclude that haruan enhances the synthesis of different glycosaminoglycans in healing wounds, which are the first component of extracellular matrix to be synthesized during the wound healing process. The enhanced levels of glycosaminoglycans may help in the formation of a resistant scar and enhanced wound contraction represents the positive influence of haruan on the fibroplastic phase of wound healing.