Pharmaceutical Field Research Articles

Preparation of Single-Helical Curdlan Hydrogel and Its Activation with Coagulation Factor G.

β-1,3-glucans are a kind of natural polysaccharide with immunomodulatory, antitumor, and anti-inflammatory properties. Curdlan, as the simplest linear β-1,3-glucan, possesses a variety of biological activities and thermogelation properties. However, due to the complexity and variability of the conformations of curdlan, the exact structure-activity relationship remains unclear. We prepare a chemically crosslinked curdlan hydrogel with the unique single-helical skeleton (named S gel) in 0.4 wt% NaOH at 40 °C, confirmed by diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS). X-ray diffractometry (XRD) data show that S gel maintains the single-helical crystal structure, and the degree of crystallinity of the S gel is ~24%, which is slightly lower than that of the raw powder (~31%). Scanning electron microscopy (SEM) reveals that S gel has a continuous network structure, with large pores measuring 50-200 μm, which is consistent with its high swelling property. Using the 13C high-resolution magic angle spinning nuclear magnetic resonance (HRMAS NMR) method, we determine that most of the single-helical skeleton carbon signals in the swollen S gel are visible, suggesting that the single-helical skeleton of S gel exhibits fascinating mobility at room temperature. Finally, we reveal that the binding of S gel to coagulation Factor G from tachypleus amebocyte lysate increases and saturates at 20 μL tachypleus amebocyte lysate per mg of S gel. Our prepared S gel can avoid the transformation of curdlan conformations and retain the bioactivity of binding to coagulation Factor G, making it a valuable material for use in the food industry and the pharmaceutical field. This work deepens the understanding of the relationship between the single-helical structure and the activity of curdlan, promoting the development and application of β-1,3-glucans.

Epigallocatechin Gallate (EGCG) Formulations: Unlocking Potential in Nutraceutical and Pharmaceutical Sectors

Objective: This review aims to evaluate the latest techniques used in formulating epigallocatechin gallate (EGCG), analyse the difficulties encountered in the process, and offer perspectives on potential future advancements in epigallocatechin gallate formulations for both nutraceutical and pharmaceutical applications. Methods: This work provides a thorough literature review, examines current epigallocatechin gallate formulation strategies, assesses solubility improvement approaches, examines obstacles, and summarises results regarding benefits and drawbacks. In nutraceuticals and pharmaceuticals, it identifies knowledge gaps and suggests future research avenues. Results: This comprehensive review presents a synthesis of pivotal discoveries about epigallocatechin gallate formulations, particularly emphasising notable progress in enhancing solubility and the concomitant obstacles encountered in this pursuit. This review weighs the benefits and drawbacks, shedding light on areas that need further investigation in the pharmaceutical and nutraceutical fields. Conclusion: This review synthesises current research on formulations containing epigallocatechin gallate, focusing on their various applications and the obstacles accompanying their implementation. Despite facing various challenges, the methodologies that have been investigated demonstrate potential. The comprehensive evaluation of both strengths and limitations highlights the imperative nature of continuous research. The conclusion provides opportunities for research in pharmaceuticals and nutraceuticals, outlining future directions.

A comprehensive assessment of machine learning algorithms for enhanced characterization and prediction in orodispersible film development

Orodispersible films (ODFs) have emerged as innovative pharmaceutical dosage forms, offering patient-specific treatment through adjustable dosing and the combination of diverse active ingredients. This expanding field generates vast datasets, requiring advanced analytical techniques for deeper understanding of data itself. Machine learning is becoming an important tool in the rapidly changing field of pharmaceutical research, particularly in drug preformulation studies. This work aims to explore into the application of machine learning methods for the analysis of experimental data obtained by ODF characterization in order to obtain an insight into the factors governing ODF performance and use it as guidance in pharmaceutical development. Using a dataset derived from extensive experimental studies, various machine learning algorithms were employed to cluster and predict critical properties of ODFs. Our results demonstrate that machine learning models, including Support vector machine, Random forest and Deep learning, exhibit high accuracy in predicting the mechanical properties of ODFs, such as flexibility and rigidity. The predictive models offered insights into the complex interaction of formulation variables. This research is a pilot study that highlights the potential of machine learning as a transformative approach in the pharmaceutical field, paving the way for more efficient and informed drug development processes.

Synergistic Outcomes in Drug Delivery: Exploring Harmonious Architectures of Interpenetrating Polymer Networks.

Pharmaceutical excipients play a crucial role in determining the outcome of delivered therapeutic cargo density. By far, polymers have captured the biggest share in the excipients market. This surge in demand motivated researchers to look for newer and novel polymeric platforms. Interpenetrating polymeric networks (IPN) are a class of polymer in the same polymer blend league, where two different polymer chains penetrate; and align with each other without any sustainable covalent bond. The novel agreement between the polymer chains equips the IPN with the characteristic features of each participating polymer unit, thus making IPN superior to its predecessors. IPN has crossed a long path, especially in the pharmaceutical medicine field, from the mere coinage of the term to widespread usage, especially in drug delivery, where they increased the bioavailability and efficacy of the co-delivered drugs. The current review will highlight the major studies that have led to the current face of the IPN in various pharmaceutical domains. The present review was conducted by comprehensively reviewing published reports within the recent period using multiple keywords related to IPN and its role in drug delivery. Moving forward, continued exploration and innovation in IPN technologies promise to further enhance their applications, offering novel solutions for the challenges in drug delivery and therapeutic cargo density.

A Comprehensive Guide to the Development, Evaluation, and Future Prospects of Self-nanoemulsifying Drug Delivery Systems for Poorly Water-soluble Drugs.

Self-nano Emulsifying Drug Delivery Systems (SNEDDS) are novel formulations that can enhance the solubility and bioavailability of poorly water-soluble drugs. SNEDDS are composed of lipids, surfactants, co-solvents, and drugs and can spontaneously form nanoemulsions when mixed with water under mild agitation. SNEDDS can be formulated as liquid or solid dosage forms and can improve drug absorption by increasing the interfacial area, protecting the drug from degradation, and facilitating lymphatic transport. SNEDDS is characterized by various parameters such as particle size, zeta potential, droplet morphology, emulsification efficiency, drug solubility, and stability. SNEDDS offers several advantages over conventional dosage forms, such as dose reduction, faster onset of action, reduced variability, versatility, and ease of formulation. However, SNEDDS also face some limitations and challenges, such as drug precipitation, cost-effectiveness, compatibility with capsule shells, and lack of predictive in vitro models. SNEDDS has a promising future in the field of pharmaceuticals, especially for personalized medicine and targeted drug delivery.

Evolution of pharmaceuticals using 3D and 4D printing

This overview examines the latest developments and applications of 3D and 4D printing in the health care and pharmaceutical field. The technique of creating a 3D object from a 3D model in the required size and shape is known as 3D printing. Whereas, 4D printing is the establishment of intricate three-dimensional formations that can change form in response to various external inputs. Applying 3D printing technology 3D printing, the healthcare industry has produced significant strides toward a patient-centred approach. The future of biomedical science and patient-centered care could be completely transformed by 3D printing with further advancements in research and development. 3D, 4D Technology used in printing is one of the most advanced industrial technologies available worldwide. The 3D and 4D printing pharmaceutical enterprises have completed the shift from centralised to distributed systems for the purpose of creating dosage forms. The goal of the study is to support the research goal of determining the degree to which patient-specific treatment is enhanced and healthcare outcomes are improved through the use of printing technologies in pharmaceuticals. Beyond this thorough analysis, the study highlights potential and problems from several angles and comparative aspects between 3D and 4D printing.

Retention mechanisms of amitriptyline and its impurities on amide, amino, diol, and silica columns in hydrophilic interaction liquid chromatography.

Hydrophilic interaction liquid chromatography (HILIC) has been widely applied to challenging analysis in biomedical and pharmaceutical fields, bridging the gap between normal-phase high-performance liquid chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC). This paper comprehensively explores the retention mechanisms of amitriptyline and its impurities A, B, C, D, F, and G on amide, amino, diol, and silica columns. Dual HILIC/RP-HPLC retention mechanisms were developed, and transitional points between HILIC and RP-HPLC mechanisms were calculated on amide, diol, and silica columns. Adsorption and partition contributions to overall retention mechanisms were evaluated using Python software in HILIC and RP-HPLC regions. The cation exchange mechanism dominates overall retention for ionized analytes in the silica column (R2>0.995), whereas the retention of ionized analytes increases with pH. Impacts of acetonitrile content, buffer ionic strength, and pH, along with their interactions on the retention of ionized analytes in the silica column, were determined using the chemometric approach. Acetonitrile content showed the most significant impact on the retention mechanisms. These findings highlight that a detailed investigation into retention mechanisms provides notable insights into factors influencing analyte retention and separation, promising valuable guidance for future analysis.

Synthon Approach in Crystal Engineering to Modulate Physicochemical Properties in Organic Salts of Chlorpropamide.

The formulation of drug with improved bioavailability is always challenging and indispensable in the field of pharmaceutics. The control of intermolecular interactions via crystal engineering approach and solid-state molecular recognition results in the formation of active drug molecules with modulated pharmacological benefits. Therefore, with the aim to improve the solubility and dissolution rate of the drug chlorpropamide (CPA), the mechanochemical liquid-assisted grinding (LAG) of the drug with several pharmaceutically accepted excipients was performed. This contributed to the discovery of six novel solid phases, namely salts, salt cocrystals and salt cocrystal hydrate─the salt of CPA with 3, 4-diaminopyridine (DAP); salt and salt cocrystal (SC) polymorph (Z″=3) with 1, 4-diazabicyclo [2.2.2] octane (DABCO); a salt, SC polymorph (Z″=9), and a SC hydrate (Z″=9) with piperazine (PIP). The formation of these salts and salt cocrystals are mainly guided by the strong hydrogen bonds with tunable strength having high electrostatic contribution. This attractive interaction brings the donor and the acceptor atoms close to each other for a facile proton transfer. Furthermore, the conformational constraints on the drug molecules, provided by the excipients via strong and directional hydrogen bonds, are quite impressive as this leads to the identification and characterization of "new conformational isomers" for the CPA molecules. The new crystalline phases exhibit enhanced intrinsic dissolution rate in comparison to that of the pure drug, the magnitude being 7, 131, and 120 folds for CPADAP, CPADABCO_II, and CPAPIP_III, respectively. Furthermore, it is interesting to note that the order of solubility is enhanced by 2.7-, 3-, and 7-fold, respectively, for the abovementioned salts. This also mirrors the trends in the magnitude of the binding energy, the higher magnitude being reflected in the lower solubility. Additionally, the in vivo experiments performed in SD rats results in the enhancement of the magnitude of the pharmacokinetic properties, when compared to the pristine drug. The concentration of the drug in CPADABCO_II and CPAPIP_III formulations exhibits 6- and 4-fold increments, respectively. Indeed, these results corroborate to the trends observed in the structural characterization, intermolecular energy calculations, solubility, and in vitro dissolution assessments.

Supramolecular Structure of the β-Cyclodextrin Metal-Organic Framework Optimizes Iodine Stability and Its Co-delivery with l-Menthol for Antibacterial Applications.

The spread of upper respiratory tract (URT) infections harms people's health and causes social burdens. Developing targeted treatment strategies for URT infections that exhibit good biocompatibility, stability, and strong antimicrobial effects remains challenging. The dual antimicrobial and antiviral effects of iodine (I2) in combination with the cooling sensation of l-menthol in the respiratory tract can simultaneously alleviate URT inflammation symptoms. However, as both I2 and l-menthol are volatile, addressing stability issues is crucial. In this study, a potassium iodide β-cyclodextrin metal-organic framework [β-CD-POF(I)] with appropriate particle size was used to coload and deliver I2 and l-menthol. Primarily, β-CD-POF(I) was employed as the most efficient carrier to significantly enhance the stability of I2, surpassing any other known protection strategies in the pharmaceutical field (CD complexations, PVP conjugations, and cadexomer iodine). The mechanism underlying the improvement in stability of I2 by β-CD-POF(I) was investigated through scanning electron microscopy with energy-dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and molecular docking. The results revealed that the key processes involved in improving stability were the inclusion of I2 by β-CD cavities in β-CD-POF(I) and the formation of polyiodide anion between iodine ions and I2. Furthermore, the potential of β-CD-POF(I) to load and deliver drugs was validated, and coloading of l-menthol and I2 demonstrated reliable stability. β-CD-POF(I) achieved a rate of URT deposition ≥95% in vitro, and the combined antibacterial effects of coloaded I2 and l-menthol was better than I2 or PVP-I alone, with no irritation noted following URT administration in rabbits. Therefore, the stable coloading of drugs by β-CD-POF(I), leading to enhanced antimicrobial effects, provides a new strategy for treating URT infections.

Antibacterial activity in vitro investigation of eucheuma cottonii extract from aru islands against pathogenic bacteria

Red algae Eucheuma cottonii is one of the potential marine biodiversity that provides added value in the pharmaceutical and cosmetic fields and is known to have antibacterial compounds. Research focusing on the potential of E. cottonii as an antibacterial is still limited, particularly when it comes to the Aru Islands. This study aimed to measure the antibacterial activity of E. cottonii against pathogenic bacteria including E. coli and S. aereus at several concentrations and to determine the best concentration for inhibiting these two pathogenic bacteria. The study was true experimental laboratory research with a post-test-only controlled group design. Antibacterial tests using the disc diffusion method and phytochemical tests were carried out. This study revealed that E. cottonii could be able to inhibit S. aureus ranging from 0.27±0.03 – 2.1±0.14 at a concentration of 50%-100%, and 0.17±0.05 – 0.45 ± 0.03 against E. coli at a concentration of 80-100%. Meanwhile, saponins and flavonoids were two bioactive compounds found through phytochemical testing. The study concluded that the n-hexane extract of E. cottonii was able to inhibit the growth of E. coli and S. aureus, even with a weak inhibition category. This study recommends that improvements are needed in the E. cottonii extraction process to maximize the antibacterial properties of E. cottonii.

A mini Review on Analytical and Bioanlytical Techniques in Study of Drugs-Protein Interaction

The choice of a suitable analytical technique for the identification of a drug or a combination of drug mainly depends on the sample matrix complexity and the analyte concentration. The appropriate choice of an analytical method is based on properties of studied analyte such as intrinsic structure and physicochemical properties etc. The extensive development of the pharmaceutical field requires more meticulous analytical methods for the control of various diseases by means of drugs. The aim of this review article was to study the drug protein interaction with respect to various analytical methods which are applied in research and innovative field of Pharmacy. The crucial factor involved and affects the binding of drugs with Body protein is distribution as well as bioactivity of drugs in the body. In biological science and medicine, the quantitative analysis of protein in biological liquids is of great importance. The aim of the review was therefore to summarize all available examples and information about the use of current analytical applications on various categories of drugs in their dosage forms and in biological samples. The different pharmaceutical active compounds, which have been determined using different methods, are summarized for the future reference of researcher.

Facile construction of pectin-based hesperidin microcapsules: Solubilization, stability, loading process, and release mechanism

Constructing carrier materials with polysaccharides to enhance the solubility of insoluble active ingredients is a crucial strategy for improving bioavailability. This research constructed pectin-based hesperidin microcapsules (PHM) through self-assembly processes in the deep eutectic solvent, improving the solubility, storage stability, and bioavailability of hesperidin (HES). PHM exhibited high encapsulation efficiency (91.7%) and loading capacity (11.5%), with a small particle size (1.73 μm). The interaction mechanism was clarified through physical characterization and density functional theory (DFT) calculations. The vitro release demonstrated that the release ratio of PHM was only 6.4% in simulated gastric fluid (SGF), but reached 80.9% in simulated intestinal fluid (SIF). The release mechanism of PHM in SGF followed Fickian diffusion, while in SIF followed skeleton dissolution diffusion with a stable rate. Furthermore, the cell cytotoxicity experiments confirmed the remarkable biocompatibility of PHM toward human colon cells, which suggested its potential application in food and pharmaceutical fields.

Solubilization study of antihypertensive drug Irbesartan in EO-PO based polymeric nanomicelle and optimization of its release behavior

Irbesartan (IRB) has low bioavailability due to its poor aqueous solubility and dissolution rate, thereby making it imperative to understand their absorption behavior. The present research work is aimed for improvement of solubilization of IRB, by investigating the effect of molecular characteristics/hydrophobicity of Pluronic® copolymers; P84 and F108 micelles with and without added salt (NaCl). The solubility of IRB was increased with copolymer concentration. Effect of added salt (NaCl) showed completely opposite behavior for both the copolymers; where F108 with NaCl has adverse effect on IRB solubilization and considerable decrease was observed. The micelle/water partition coefficient and the standard free energy change (∆G◦) were also evaluated from thermodynamic view point. The in vitro release kinetics of IRB drug was carried out for 1% P84 with and without 1 M NaCl and compared with its release in water. It was found that P84/NaCl reduces the release rate more efficiently for IRB compared to other systems. There are few reports about the solubilization improvement of IRB; but this is the first time where the study of Pluronic® micelle carried out for IRB solubilization with varying solvent condition as per best of our knowledge. This information can be useful to understand the solubilization behaviour of Pluronic® copolymers available in varieties of molecular weights and can be used to identify possible solvent condition for newer applications of Pluronic® copolymers in pharmaceutical field.

Development and Efficacy Evaluation of Innovative Cosmetic Formulations with Caryocar brasiliense Fruit Pulp Oil Encapsulated in Freeze-Dried Liposomes.

Encapsulation and drying technologies allow the engineering of innovative raw materials from plant biodiversity, with potential applications in pharmaceutical and cosmetic fields. Lipid-based nanoencapsulation stands out for its efficiency, ease of production, and versatility in encapsulating substances, whether hydrophilic or lipophilic. This work aimed at encapsulating pequi oil in liposomes and freeze-dried liposomes to enhance its stability and functional benefits, such as skin hydration and anti-aging effects, for use in innovative cosmetic formulations. Pequi oil-extracted from the Caryocar brasiliense fruit pulp, a plant species from Brazilian plant biodiversity-is rich in secondary metabolites and fatty acids. Liposomes and dried liposomes offer controlled production processes and seamless integration into cosmetic formulations. The physicochemical analysis of the developed liposomes confirmed that the formulations are homogeneous and electrokinetically stable, as evidenced by consistent particle size distribution and zeta potential values, respectively. The gel-type formulations loaded with the dried liposomes exhibit enhanced skin hydration, improved barrier function, and refined microrelief, indicating improvements in skin conditions. These results highlight the potential of dried liposomes containing pequi oil for the development of innovative cosmeceutical products. This research contributes to the valorization of Brazilian biodiversity by presenting an innovative approach to leveraging the dermatological benefits of pequi oil in cosmetic applications.

Advances in Enzymatic Incorporation of Small Fluorine Modules

AbstractOrganofluorine compounds find extensive application in the fields of agrochemicals, pharmaceuticals, materials science, and molecular imaging. Introducing fluorine atoms can provide organic compounds with unique physicochemical properties or improve their biological activity. Although significant progress has been made in the chemical synthesis of fluorine‐containing compounds, achieving selective fluorination under mild conditions remains extremely challenging. Introducing biocatalytic approaches in organofluorine chemistry is an important strategy given their high efficiency, selectivity, and environmental friendliness. In this review, we present the discovery of fluorinated natural products and fluorinases, the crystal structure and the directed evolution of fluorinases, with a focus on recent advances in the enzymatic synthesis of fluorine‐containing compounds in recent years. It is hoped that this review will help to promote the field of biocatalytic organofluorine compound synthesis.

Degradation-Based Protein Profiling: A Case Study of Celastrol.

Natural products, while valuable for drug discovery, encounter limitations like uncertainty in targets and toxicity. As an important active ingredient in traditional Chinese medicine, celastrol exhibits a wide range of biological activities, yet its mechanism remains unclear. In this study, they introduced an innovative "Degradation-based protein profiling (DBPP)" strategy, which combined PROteolysis TArgeting Chimeras (PROTAC) technology with quantitative proteomics and Immunoprecipitation-Mass Spectrometry (IP-MS) techniques, to identify multiple targets of natural products using a toolbox of degraders. Taking celastrol as an example, they successfully identified its known targets, including inhibitor of nuclear factor kappa B kinase subunit beta (IKKβ), phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PI3Kα), and cellular inhibitor of PP2A (CIP2A), as well as potential new targets such as checkpoint kinase 1 (CHK1), O-GlcNAcase (OGA), and DNA excision repair protein ERCC-6-like (ERCC6L). Furthermore, the first glycosidase degrader is developed in this work. Finally, by employing a mixed PROTAC toolbox in quantitative proteomics, they also achieved multi-target identification of celastrol, significantly reducing costs while improving efficiency. Taken together, they believe that the DBPP strategy can complement existing target identification strategies, thereby facilitating the rapid advancement of the pharmaceutical field.

Catalytic Approaches to the Halogen Dance Reaction for Molecular Editing

AbstractTransposition reactions, i. e., switching the position of functional groups, are a powerful tool for molecular editing. The transposition of a halogen atom attached to an aromatic ring, referred to as the halogen dance reaction, has great potential in the fields of pharmaceuticals, agrochemicals, natural products, and functional materials. However, the substrate scope of this reaction is limited owing to the intrinsic difficulty in facilitating multiple catalytic cycles involving several aryllithium species. In this concept paper, recent advances in halogen dance catalysis that have expanded the substrate scope of this reaction are highlighted.

Therapeutic Effects of Essential Oils and Their Bioactive Compounds on Prostate Cancer Treatment.

Since prostate cancer (PCa) relies on limited therapies, more effective alternatives are required. Essential oils (EOs) and their bioactive compounds are natural products that have many properties including anticancer activity. This review covers studies published between 2000 and 2023 and discusses the anti-prostate cancer mechanisms of the EOs from several plant species and their main bioactive compounds. It also provides a critical perspective regarding the challenges to be overcome until they reach the market. EOs from chamomile, cinnamon, Citrus species, turmeric, Cymbopogon species, ginger, lavender, Mentha species, rosemary, Salvia species, thyme and other species have been tested in different PCa cell lines and have shown excellent results, including the inhibition of cell growth and migration, the induction of apoptosis, modulation in the expression of apoptotic and anti-apoptotic genes and the suppression of angiogenesis. The most challenging aspects of EOs, which limit their clinical uses, are their highly lipophilic nature, physicochemical instability, photosensitivity, high volatility and composition variability. The processing of EO-based products in the pharmaceutical field may be an interesting alternative to circumvent EOs' limitations, resulting in several benefits in their further clinical use. Identifying their bioactive compounds, therapeutic effects and chemical structures could open new perspectives for innovative developments in the field. Moreover, this could be helpful in obtaining versatile chemical synthesis routes and/or biotechnological drug production strategies, providing an accurate, safe and sustainable source of these bioactive compounds, while looking at their use as gold-standard therapy in the close future.

High throughput microfluidics-based synthesis of PEGylated liposomes for precise size control and efficient drug encapsulation

The low scalability and reproducibility of existing synthesis methods have hindered the translation of liposome nanoparticles as carriers for targeted drug delivery from conventional laboratory techniques to mass production. To this end, in this study, we present a high-throughput microfluidics-based approach for the synthesis of PEGylated liposomes with a primary focus on achieving precise size control and efficient encapsulation of hydrophobic drug molecules. In this platform, liposomes were self-assembled through a controllable mixing of lipids (EYPC, cholesterol, and DSPE-PEG 2000) dissolved in ethanol and an aqueous solution. The key parameters, including the chip design, total flow rate, flow rate ratio, lipid concentrations, as well as variations in buffer (HEPES and NaCl) and solvent composition (commercial and reagent-grade ethanol) were explored in detail. Through comprehensive parametric studies, we gained valuable insights into the influence of these variables on the size distribution of liposomes and succeeded in producing highly reproducible liposomes ranging from approximately 60 nm (corresponding to small unilamellar vesicles) to 150 nm (representing large unilamellar vesicles), all while maintaining a polydispersity index (PDI) of less than 0.2. To assess the encapsulation efficiency of hydrophobic drug molecules, Nile red (NR) was employed as a surrogate. We meticulously examined the impact of NR concentration on the drug encapsulation process, resulting in up to 74% drug encapsulation efficiency within the PEGylated liposomes. This research offers crucial advances in liposome synthesis and drug delivery, providing a high-throughput, controllable method for PEGylated liposomes with potential in pharmaceutical and biomedical fields.

Development of redispersible phytoglycogen and zein nanocomplexes for encapsulation of bioactive phytochemicals and their antimicrobial activities

In this study, hydrophobically modified phytoglycogen and hydrolyzed zein were utilized to develop biodegradable and redispersible nanocomplexes for encapsulation of various bioactive phytochemicals, including eugenol, thymol, carvacrol, trans-cinnamaldehyde, and their mixtures. Comprehensive physicochemical and colloidal characterizations of these nanocomplexes were performed using dynamic light scattering, and Fourier transform infrared and atomic force microscopy, which evaluated their colloidal stability and redispersibility after freeze-drying. In addition, the antioxidant capacities and antimicrobial activities of these various nanoparticles were investigated methodically. The results revealed that the phytochemical-encapsulated nanocomplexes exhibited a particle size ranging from 60 to 70 nm, displaying a uniform distribution. Additionally, these nanocomplexes demonstrated sustained morphological and colloidal stability over 30-day storage, and after freeze-drying and subsequent redispersion processing. Furthermore, significant improvements in antioxidant and antimicrobial properties in the aqueous phase were observed. These redispersible nanocomplexes from phytoglycogen and zein hold the promising potential to address the limitations and offer practical solutions for expanding their applications in the pharmaceutical and food fields.