Concentrations Of Pharmaceutically Active Compounds Research Articles

Occurrence and human risk assessment of pharmaceutically active compounds (PhACs) in indoor dust from homes, schools and offices

This study investigates the current situation and possible health risks due to pharmaceutically active compounds (PhACs) including analgesics, antibiotics, antifungals, anti-inflammatories, psychiatric and cardiovascular drugs, and metabolites, in indoor environments. To achieve this objective, a total of 85 dust samples were collected in 2022 from three different Spanish indoor environments: homes, classrooms, and offices. The analytical method was validated meeting SANTE/2020/12830 and SANTE/12682/2019 performance criteria. All indoor dust samples except one presented at least one PhAC. Although concentration levels ranged from < LOQ to 18 µg/g, only acetaminophen, thiabendazole, clotrimazole, and anhydroerythromycin showed quantification frequencies (Qf %) above 19% with median concentrations of 166 ng/g, 74 ng/g, 25 ng/g and 14 ng/g, respectively. The PhAC distribution between dust deposited on the floor and settled on elevated (> 0.5 m) surfaces was assessed but no significant differences (p > 0.05, Mann–Whitney U-test) were found. However, concentrations quantified at the three types of locations showed significant differences (p < 0.05, Kruskal–Wallis H-test). Homes turned out to be the indoor environment with higher pharmaceutical concentrations, especially acetaminophen (678 ng/g, median). The use of these medicines and their subsequent removal from the body were identified as the main PhAC sources in indoor dust. Relationships between occupant habits, building characteristics, and/or medicine consumption and PhAC concentrations were studied. Finally, on account of concentration differences, estimated daily intakes (EDIs) for inhalation, ingestion and dermal adsorption exposure pathways were calculated for toddlers, adolescents and adults in homes, classrooms and offices separately. Results proved that dust ingestion is the main route of exposure, contributing more than 99% in all indoor environments. Moreover, PhAC intakes for all studied groups, at occupational locations (classrooms and offices) are much lower than that obtained for homes, where hazard indexes (HIs) obtained for acetaminophen (7%—12%) and clotrimazole (4%-7%) at the worst scenario (P95) highlight the need for continuous monitoring.

HRMS-based suspect screening of pharmaceuticals and their transformation products in multiple environmental compartments: An alternative to target analysis?

The comprehensive monitoring of pharmaceutically active compounds (PhACs) in the environment is challenging given the myriad of substances continuously discharged, the increasing number of new compounds being produced (and released), or the variety of the associated human metabolites and transformation products (TPs). Approaches such as high-resolution mass spectrometry (HRMS)-based suspect analysis have emerged to overcome the drawbacks of classical target analytical methods, e.g., restricted chemical coverage. In this study, we assess the readiness of HRMS-based suspect screening to replace or rather complement target methodologies by comparing the performance of both approaches in terms of i) detection of PhACs in various environmental samples (water, sediments, biofilm, fish plasma, muscle and liver) in a field study; ii) PhACs (semi)quantification and iii) prediction of their environmental risks. Our findings revealed that target strategies alone significantly underestimate the variety of PhACs potentially impacting the environment. However, relying solely on suspect strategies can misjudge the presence and risk of low-level but potentially risky PhACs. Additionally, semiquantitative approaches, despite slightly overestimating concentrations, can provide a realistic overview of PhACs concentrations. Hence, it is recommended to adopt a combined strategy that first evaluates suspected threats and subsequently includes the relevant ones in the established target methodologies.

Pharmaceutically active compounds (PhACs) in surface water: Occurrence, trends and risk assessment in the Tagus River Basin (Spain)

In this study, the presence of 23 pharmaceutically active compounds (PhACs) including antibiotics, analgesics, anti-inflammatories, psychiatric and cardiovascular drugs, antifungals and metabolites was investigated in surface waters. A total of 89 samples were collected during 3 years (2020, 2021 and 2022) from a European representative river basin (Tagus, Spain). To elucidate PhAC potential sources, sampling points located in areas with low, median and high anthropogenic influence were selected. The analytical method based on solid phase extraction (SPE) followed by UHPLC-MS/MS analysis was validated meeting SANTE/2020/12830 and SANTE/12682/2019 performance criteria. PhACs were quantified above limits of quantification (LOQs) in 96 % of water samples, being the antihypertensives valsartan (648 ng/L, 87 % quantification frequency) and irbesartan (390 ng/L, 75 %) and the antidepressant o-desmethylvenlafaxine (495 ng/L, 76 %) the predominant pollutants. The rest of the target PhACs showed median concentrations between 4 and 172 ng/L with quantification frequencies ranging from 35 to 75 %. ∑PhAC concentrations did not show temporal or seasonal trends. However, valsartan and naproxen presented lower levels in drier (spring and summer) compared to the wetter. Source identification revealed a clear anthropogenic origin since concentrations obtained in highly populated areas were statistically higher (p < 0.01) than those quantified in sparsely populated ones. This finding was also confirmed by calculating PhACs mass flow rates, which ranged between 1.4 and 235 kg/y. Finally, data generated were used to estimate the potential risk to the aquatic ecosystem for three trophic levels (phototrophic, invertebrate and vertebrate organisms). Risk quotient ratios (RQs) were calculated for all PhACs at the median (P50) and worst-case (max) scenarios. Up to 7 PhACs (acetaminophen, carbamazepine, gemfibrozil, ibuprofen, irbesartan, ketoprofen and venlafaxine) showed high risk for the highest trophic level (fish) in >45 % of investigated locations.

First evidence of environmental bioaccumulation of pharmaceuticals on adult native anurans (Rhinella arenarum) from Argentina

The presence of pharmaceutically active compounds (PhACs) in surface water is well known, whereas their natural occurrence in biota is much less explored. The aim of this work was to evaluate the bioaccumulation of PhACs in adult toads of the neotropical species Rhinella arenarum. Three sites were selected in Buenos Aires (Argentina): a reference site (Site 1), a site with direct discharge from a secondary wastewater treatment plant (WWTP) (Site 2) and a site 300 m downstream of the WWTP discharge (Site 3). Surface water samples, as well as muscle, liver and fat bodies of toads were collected, extracted and analyzed by LC-MS/MS. Highly significant differences in total PhACs concentration in surface water (p < 0.005) were detected between Site 2 and the other sites. These concentrations ranged from 0.37 to 52.46 ng/L at Site 1, 0.71–6950.37 ng/L at Site 2, and 0.12–75.45 ng/L at Site 3. In general, bioaccumulation of PhACs in toad tissues was similar between sites and tissues of each site. The highest concentrations were detected in the muscle of toads from Site 3 (1.06–87.24 ng/g dw), followed by liver (1.77–38.10 ng/g dw) and fat bodies (0.68–20.59 ng/g dw) from Site 1. Ibuprofen (6950 ng/L), acetaminophen (3277 ng/L) and valsartan (2504 ng/L) were the compounds with the highest concentrations in surface water from Site 2, whereas acetaminophen (87.2 ng/g dw, muscle from Site 3), desloratadine (38.1 ng/g dw, liver from Site 1), and phenazone (25.9 ng/g dw, liver from Site 1) were the ones that showed the highest concentrations in biota. This is the first time a field study has examined the environmental bioaccumulation of PhACs in anurans, demonstrating their potential for monitoring the status of natural ecosystems.

Ocorrência e avaliação de risco ecológicos de compostos farmaceuticamente ativos em pequenas bacias neotropicais, Brasil

Abstract: Aim The aim of our study was to evaluate the contamination levels of selected pharmaceutically active compounds (PAC) and their potential ecological threat to forested streams. Methods Samples of stream water were collected in the second largest city in Mato Grosso do Sul State in center-west of Brazil. Physicochemical parameters and concentrations of PAC were quantified in samples collected in six field campaigns. Ecological risk assessment (ERA) based on risk quotient (RQ) was performed based on the maximum measured concentration of PAC in water. Results Six pharmaceutical compounds were successfully quantified in the forested streams, namely caffeine, naproxen, diclofenac, estriol, estradiol and ethinylestradiol. From the point of view of ecological risk, ethinylestradiol (22,57), estradiol (1,46), diclofenac (16.99) and caffeine (5.30) can be considered as priorities PAC, as they present moderate to high risks to aquatic organisms and may also cause damage to the food chain. Conclusions This study provides valuable information to emphasize the importance of continuous monitoring of forested streams in the west central region of the country, as well as efforts to control the input of these micropollutants into watercourses.

Pharmaceutically active compounds in biotic and abiotic media of rivers receiving urban sewage: Concentrations, bioaccumulation and ecological risk

Pharmaceutically active compounds (PhACs) are recognized as posing health risks to aquatic ecosystems. The occurrence characteristics and ecological effects of 15 PhACs were investigated in the multiphase media of Yangtze River (Nanjing area, China) during wet and dry seasons. All 15 PhACs were detected in the traditionally dissolved phase, suspended particulate matter, sediment and fish, with total concentrations of PhACs (ΣPhACs) of 64.75–366.20 ng/L, 18.50–69.40 ng/g, 3.29–23.30 ng/g and 0.00–176.44 ng/g, respectively. Ibuprofen (IPF), roxithromycin (ROX) and caffeine (CFI) were the dominant PhACs in all of the environmental media. As the main carrier for PhACs, the colloids contributed 8.60%− 77.04% of PhACs in the traditionally dissolved phase, and distribution coefficients (log Kcol=6.38–7.95) of PhACs in colloidal phase were significantly higher than those in SPM and sediment. The bioaccumulation potential of ΣPhACs in the wild fish tissues was generally in order as follows: brain > liver > kidney > gill > blood > intestines > muscle > bile, which was positively correlated with their log Kow. The concentrations of CFI, ROX and IPF in the muscle, gill and liver can be predicted by their log Kow and pollution levels in the blood, and the concentrations of PhACs in the muscle of benthic fish were much higher than those in pelagic fish. The bioaccumulation factors of ROX, fluoxetine and bezafibrate in intestines, brain and blood showed strong enrichment capacity (>5000). In the risk assessment, the acute risk level of PhACs to aquatic organisms decreases gradually with the trophic level, while the trend of chronic risk was the opposite. The chronic mixture risk of PhACs to fish was > 1 at all sampling sites, 59.79% of mixed risk quotient were provided by clofibric acid, diclofenac, erythromycin and ketoconazole. These results indicated that PhAC can cause harmful health effects to sensitive organisms both individually and mixedly.

Pharmaceuticals in water and sediment of small streams under the pressure of urbanization: Concentrations, interactions, and risks

Small streams are crucial but vulnerable elements of ecological networks. To better understand the occurrence of pharmaceutically active compounds (PhACs) in streams, this study focused on the occurrence, distribution, and environmental risk of 111 PhACs and 7 trace elements based on a total of 141 water and sediment samples from small streams located in the urbanizing region of Budapest, Hungary. Eighty-one PhACs were detected in the aqueous phase, whereas sixty-two compounds were detected in the sediment. Carbamazepine (CBZ) was the most frequently identified PhAC in water, and was found in 91.5% of all samples. However, the highest concentrations were measured for lamotrigine (344.8 μg·L−1) and caffeine (221.4 μg·L−1). Lidocaine was the most frequently occurring PhAC in sediment (73.8%), but the maximum concentrations were detected for CBZ (395.9 ng·g−1) and tiapride (187.7 ng·g−1). In both water and sediment, more PhACs were found downstream of the wastewater treatment plants (WWTPs) than in the samples not affected by treated wastewater, even though no relationship was observed between the total amount of treated wastewater and the number of detected PhACs. The PhAC concentrations were also independent of the distance from the WWTP effluents. PhAC-polluted samples were detected upstream of the WWTPs, thereby suggesting the relevance of diffuse emissions in addition to WWTP outlets. The most frequently detected PhACs in the sediment were usually also present in the water samples collected at the same place and time. The varying concentrations of PhACs and the fluctuating water–sediment properties resulted in a lack of correlation between the general chemical properties and the concentrations of PhACs, which makes it difficult to predict PhAC contamination and risks in urbanized small streams. The environmental risk assessment indicated that diclofenac had the highest risk in the sampling area.

Occurrence of Pharmaceutically Active Compounds and Potential Ecological Risks in Wastewater from Hospitals and Receiving Waters in Sri Lanka.

The presence of pharmaceutically active compounds (PACs) in the environment and their associated hazards is a major global health concern; however, data on these compounds are scarce in developing nations. In the present study, the existence of 39 non-antimicrobial PACs and six of their metabolites in wastewater from hospitals and adjacent surface waters in Sri Lanka was investigated from 2016 to 2018. The highest amounts of the measured chemicals, including the highest concentrations of atorvastatin (14,620 ng/L) and two metabolites, mefenamic acid (12,120 ng/L) and o-desmethyl tramadol (8700 ng/L), were detected in wastewater from the largest facility. Mefenamic acid, gemfibrozil, losartan, cetirizine, carbamazepine, and phenytoin were detected in all the samples. The removal rates in wastewater treatment were 100% for zolpidem, norsertaline, quetiapine, chlorpromazine, and alprazolam. There was substantial variation in removal rates of PACs among facilities, and the overall data suggest that treatment processes in facilities were ineffective and that some PAC concentrations in the effluents were increased. The estimated risk quotients revealed that 14 PACs detected in water samples could pose low to high ecological risk to various aquatic organisms. Compounds such as ibuprofen, tramadol, and chlorpromazine detected in untreated and treated wastewater at these facilities pose a high risk to several aquatic organisms. Our study provides novel monitoring data for non-antimicrobial PAC abundance and the associated potential ecological risk related to hospitals and urban surface waters in Sri Lanka and further offers valuable information on pre-COVID-19 era PAC distribution in the country. Environ Toxicol Chem 2022;41:298-311. © 2021 SETAC.

Occurrence and health risk assessment of pharmaceutically active compounds in riverbank filtrated drinking water

The presence of pharmaceutically active compounds (PhACs) in drinking waters might pose a serious threat to human health worldwide. Therefore, this study sought to measure PhACs in Danube-derived tap water from the Budapest metropolitan region (Hungary), and to compare the results of those measured in the bank filtrate after which a human health risk assessment (based on human risk quotient [hRQ]) was conducted for the detected PhACs. A total of 108 samples were collected from 21 sampling sites throughout 6 sampling campaigns. Our study screened for 102 PhACs, of which 19 were detected in the persistently chlorinated tap water samples. PhAC concentrations were much lower than previously assumed based on the contamination of raw water resources. The total mean concentration of the analyzed PhACs exceeded 30 ng L−1 only at 5 sites. Moreover, the frequency of occurrence (FRO) of the 6 most common compounds (carbamazepine, lamotrigine, lidocaine, benzoylecgonine, tramadol, and cinolazepam) reached 50 % at 4 sites. The most frequent PhAC was carbamazepine (FRO = 53.7 %), the risk level of all PhACs investigated was negligible (hRQ<1) with carbamazepine having the highest hRQs (hRQMAX = 0.007; hRQMEAN = 0.001). Tap water provided lower PhAC concentrations farther from the water abstraction wells and treatment stations along the Danube. The travel time between the drinking water wells and taps with other factors, such as the varying microbiological pattern and the deposits in the supply system influence the PhAC concentrations. Based on the risk assessment, all investigated PhACs pose a negligible risk to consumers in the investigated urban area.

Effects of pharmaceutically active compounds (PhACs) on fish body and scale shape in natural waters.

BackgroundIn recent years, there are growing concerns about pharmaceutically active compounds (PhACs) in natural ecosystems. These compounds have been found in natural waters and in fish tissues worldwide. Regarding their growing distribution and abundance, it is becoming clear that traditionally used risk assessment methodologies and ecotoxicological studies have limitations in several respects. In our study a new, combined approach of environmental impact assesment of PhACs has been used.MethodsIn this study, the constant watercourses of the suburban region of the Hungarian capital (Budapest) were sampled, and the body shape and scale shape of three fish species (roach Rutilus rutilus, chub Squalius cephalus, gibel carp Carassius gibelio) found in these waters were analyzed, based on landmark-based geometric morphometric methods. Possible connections were made between the differences in body shape and scale shape, and abiotic environmental variables (local- and landscape-scale) and measured PhACs.ResultsSignificant connections were found between shape and PhACs concentrations in several cases. Despite the relatively large number of compounds (54) detected, citalopram, propranolol, codeine and trimetazidine significantly affected only fish body and scale shape, based on their concentrations. These four PhACs were shown to be high (citalopram), medium (propranolol and codeine), and low (trimetazidine) risk levels during the environmental risk assessment, which were based on Risk Quotient calculation. Furthermore, seven PhACs (diclofenac, Estrone (E1), tramadol, caffeine 17α-Ethinylestradiol (EE2), 17α-Estradiol (aE2), Estriol (E3)) were also categorized with a high risk level. However, our morphological studies indicated that only citalopram was found to affect fish phenotype amongst the PhACs posing high risk. Therefore, our results revealed that the output of (traditional) environmental/ecological risk assessment based on ecotoxicological data of different aquatic organisms not necessarily show consistency with a “real-life” situation; furthermore, the morphological investigations may also be a good sub-lethal endpoint in ecotoxicological assessments.

Post-treatment of real municipal wastewater effluents by means of granular activated carbon (GAC) based catalytic processes: A focus on abatement of pharmaceutically active compounds

Pharmaceutically active compounds (PhACs) widely present in urban wastewater effluents pose a threat to ecosystems in the receiving aquatic environment. In this work, efficiency of granular activated carbon (GAC) - based catalytic processes, namely catalytic wet peroxide oxidation (CWPO), peroxymonosulfate oxidation (PMS/GAC) and peroxydisulfate oxidation (PDS/GAC) at ambient temperature and pressure were studied for removal of 22 PhACs (ng L−1 level) that were present in secondary effluents of real urban wastewater. Concentrations of PhACs were measured using Ultra Performance Liquid Chromatography – Triple Quadrupole Mass Spectrometry (UPLC-QqQ-MS/MS). Catalytic experiments were conducted in discontinuous mode using up-flow fixed bed reactors with granular activated carbon (GAC) as a catalyst. The catalyst was characterized by means of N2 adsorption-desorption isotherm, mercury intrusion porosimetry (MIP), elemental analysis, X-ray fluorescence spectroscopy (WDXRF), X-ray diffraction (XRD), thermal gravimetry and differential temperature analyses coupled mass spectrometry (TGA-DTA-MS). Results indicate that the highest efficiency in terms of TOC removal was achieved during CWPO performed at optimal operational conditions (stoichiometric dose of H2O2; TOC removal ~ 82%) followed by PMS/GAC (initial PMS concentration 100 mg L−1; TOC removal ~73.7%) and PDS/GAC (initial PDS concentration 100 mg L−1; TOC removal ~ 67.9%) after 5 min of contact time. Full consumption of oxidants was observed in all cases for CWPO and PDS/GAC at contact times of 2.5 min, while for PMS/GAC it was 1.5 min. In general, for 18 out of 22 target PhACs, very high removal efficiencies (> 92%) were achieved in all tested processes (including adsorption) performed at optimal operational conditions during 5 min of contact time. However, moderate (40 – 70%) and poor (< 40%) removal efficiencies were achieved for salicylic acid, ofloxacin, norfloxacin and ciprofloxacin, which can be possibly attributed to insufficient contact time. Despite high efficiency of all studied processes for PhACs elimination from urban wastewater effluent, CWPO seems to be more promising for continuous operation.

Seasonal occurrence and source analysis of pharmaceutically active compounds (PhACs) in aquatic environment in a small and medium-sized city, China

Pharmaceutically active compounds (PhACs) have been widely reported in most megacities in China but seldom in small and median-sized cities. The aim of this study is to investigate occurrence and distribution characteristics of PhACs in a typical small and medium-sized city in China and analyze their sources. 33 PhACs and 4 chiral drugs were selected for a four-season monitoring campaign in Wujin District, Jiangsu Province, a typical small and median-sized city, in 2018. PhACs concentration level in surface water, ranging from ng L−1 to μg L−1, was lower than in large-sized cities and areas in China. Impact from agricultural sources should be concerned in the study area: (1) Significant correlation between concentrations of antibiotics and NH4-N in surface water indicated the potential impact from agricultural sources (fishponds and livestock farms); (2) Government regulating measures on livestock and poultry farms since January 2018 have effectively decreased macrolides and lincosamides emissions into surface water. As for source analysis, CF/CBZ (Caffeine/Carbamazepine) was a feasible indicator to trace untreated wastewater and enantiomeric fraction values of metoprolol (MTP) were also suggested to be helpful for identifying untreated wastewater. The results of both two indicators indicated more input of untreated wastewater to surface water in the northeast area and upstream of Wuyi Canal. To our best knowledge, this is the first study to systematically analyze PhACs in aquatic environment for a small and medium-sized city in China.

Assessing pharmaceutical contamination along the Mediterranean and Red Sea coasts of Israel: Ascidians (Chordata, Ascidiacea) as bioindicators

Global increase in the use of pharmaceutically-active compounds (PhACs), and their insufficient removal in wastewater treatment plants, have resulted in their continuous release into the marine environment. We investigated the use of the solitary ascidians Herdmania momus, Microcosmus exasperatus, and Styela plicata as bioindicators of three common PhACs in the Israeli coastal waters: Bezafibrate, carbamazepine and diclofenac. Both the Mediterranean and the Red-Sea coasts were found contaminated with PhACs, detected at all 11 sampling sites, with four sites contaminated with all three studied PhACs. Diclofenac was most frequent, present in nine of the 11 sites with concentrations reaching 51.9 ng/g of dry weight sample (dw). Bezafibrate and carbamazepine reached concentrations of 47.8 ng/g dw and 14.3 ng/g dw, respectively. The alarming detection of such high concentrations of PhACs in ascidians along Israel's coasts demonstrates both the extent of PhACs contamination in the region, and the potential of ascidians as bioindicators, and emphasizes the urgent need for additional research into PhAC contamination sources and effects.

Dataset of pharmaceuticals in the Danube and related drinking water wells in the Budapest region.

The present dataset provides data on the pharmaceutically active compounds (PhACs) concentrations measured in the Danube and the drinking water abstraction wells (DWAW) in the Budapest region. Grab samples were collected during five periods. One hundred and seven water samples from the Danube and ninety water samples from the relevant DWAWs were analyzed to quantify physical-chemical parameters, trace element concentrations, and one hundred and eleven PhACs, including pharmaceutical derivatives, illicit drugs, and alkaloids. The ion concentrations were measured using dual channel ion chromatography, spectrophotometric and titrimetric methods, and inductively coupled plasma mass spectrometry. PhACs concentrations were measured after solid-phase extraction applying supercritical fluid chromatography coupled with tandem mass spectrometry. Fifty-two PhACs were quantified in the Danube, and ten PhACs were present in >80% of the samples. Whereas thirty-two PhACs were quantified in the DWAWs. The present dataset is useful for further comparisons and meta-analyses.

Pharmaceutically active compounds (PhACs) in surface sediments of the Jiaozhou Bay, north China

Pharmaceutically active compounds (PhACs) have attracted increasing attention due to their large consumption volumes, high bioactivity and potential ecotoxicity. In this study, a total of 150 commonly used drugs were investigated in sediments of Jiaozhou Bay (JZB). Twenty-five target compounds were detected, of which ten were discovered for the first time in marine sediments. The range of total PhAC content was 3.62–21.4 ng/g dry weight. Ketoprofen (2.49 ng/g), oxytetracycline (1.00 ng/g) and roxithromycin (0.97 ng/g) were the preponderant PhACs. PhACs gradually decreased from east to west, and the distribution of PhACs in the sediment was controlled by the source channel, seawater dynamic process and sediment composition. The diatom, organic matter, and clay proportions in the sediments and the nutrients in the overlying water were the most important environmental factors affecting the distribution of PhACs. PhAC pollution in the sediments of the JZB exhibited an increasing trend. Coprostanol could be used as a chemical indicator of the PhAC concentration in JZB sediments. PhACs were mainly derived from direct pollution due to human fecal excretion in the eastern region. Ofloxacin, tetracycline and oxytetracycline were found to pose high or medium risks to aquatic organisms. It is necessary and urgent to improve the treatment technology of drug residues in sewage treatment plants to decrease the pollution of PhAC residues. With the continuous aging of the global population, the use of PhACs will increase rapidly, which may cause more unpredictable threats to the marine ecosystem. Therefore, the monitoring of PhACs in the marine environment needs to be strengthened, and studies on PhAC occurrence and effects must be considered a priority in global environmental research.

Thermal baths as sources of pharmaceutical and illicit drug contamination

Despite the fact that there are tens of thousands of thermal baths in existence, knowledge about the occurrence of pharmaceutically active compounds (PhACs) in untreated thermal wastewater is very limited. Because used thermal water is typically legally discharged into surface waters without any treatment, the effluent poses environmental risks for the receiving water bodies. The aim of this study was to show the occurrence patterns and spatiotemporal characteristics of 111 PhACs in thermal wastewater. Six thermal water outflows of different thermal baths were tested in different seasons in the Budapest metropolitan region (Hungary), and diurnal analysis was performed. After solid-phase extraction, the samples were analysed and quantified by coupling supercritical fluid chromatography and mass spectrometry to perform simultaneous multi-residue drug analysis. The results confirm that water discharge pipes directly transport pharmaceuticals into surface water bodies; 34 PhACs were measured to be over the limit of quantification at least once, and 21 of them were found in more than one water sample. The local anaesthetic drug lidocaine, antiepileptic carbamazepine, analgesic derivative tramadol and illicit drug cocaine were detected in more than half of the samples. Caffeine, metoprolol and bisoprolol (cardiovascular drugs), benzoylecgonine (cocaine metabolite), diclofenac (NSAID), citalopram (antidepressant) and certain types of hormones also have a significant frequency of 30-50%. However, the occurrence and concentrations of PhACs vary according to the season and number/types of visitors. As demonstrated by the diurnal fluctuation, drug contamination of thermal waters can significantly vary, even for similar types of baths; furthermore, the quantity and types of some pollutants rapidly change in the discharged thermal wastewater.

Biogeochemical characteristics and ecological risk assessment of pharmaceutically active compounds (PhACs) in the surface seawaters of Jiaozhou Bay, North China

The occurrence and distribution of 168 pharmaceutically active compounds (PhACs) in the surface seawater of Jiaozhou Bay (JZB) were investigated using ultra-high-performance liquid chromatography in tandem with a triple-quadrupole mass spectrometer equipped with an electrospray ionization source (UHPLC-ESI-MS-MS). Thirty-six compounds were detected, and 17 of these compounds were first detected in seawater, including sulfabenzamide, sulphacetamide, cephalonium, desacetyl-cefotaxime, cefminox, cefotaxime, cephradine, cefazolin, carprofen, nabumetone, glibenclamide, glimepiride, glipizide, prednisone, fluoromethalone, diazepam and amantadine. The total concentration of PhACs in the surface seawater ranged from 23.6 ng/L to 217 ng/L. The compounds found at the highest mean concentrations included amantadine (24.7 ng/L), lincomycin (8.55 ng/L), carprofen (8.30 ng/L), and tetracycline (7.48 ng/L). The PhAC concentration was higher in the inner bay than in the outside of the bay. In the inner bay, the eastern district showed higher concentrations of PhACs than the western district. Input from the Licun River may be the primary source of pollution. A statistically significant positive correlation was observed between nutrients and PhACs in seawater. Phosphate can be used to indicate the distribution of PhACs in JZB. Based on the individual risk quotient (RQ) values, lincomycin and ofloxacin posed high risks to the relevant aquatic organisms in JZB, especially in the eastern parts. Regular monitoring is required to evaluate the levels of PhACs as they are constantly released into JZB.

Amendment of municipal sewage sludge with lime and mussel shell: Effects on fate of organic matter and pharmaceutically active compounds

The deterioration in its strength from long-term degradation of organic matter and release of pharmaceutically active compounds (PhACs) have caused adverse environmental effects in municipal sewage sludge (MSS) landfill. Lime and a mixture of lime and mussel shell were employed as potential stabilization agents for MSS in this work. Their efficacy was assessed by investigating the effects on transformation of organic matter, as well as the occurrence and fate of four PhACs (fluoxetine, gemfibrozil, triclosan and carbamazepine) over 42 days. The addition of the selected agents: (i) prevented the microbial degradation of organic matter; (ii) modified the predominant functional groups of amide groups (amide I and II) and polysaccharides to deprotonated carboxylic groups and destruction of amide groups; and (iii) shifted the abundance of organic constituents from microbial by-products to humic acid-like organics with conformational changes. The measurement method provided reliable and precise results for determining PhAC concentrations in MSS with and without amendment, although matrix effects and process effects were found to affect measurement sensitivity. Available fractions of the PhACs increased in MSS with lime addition, but decreased in the presence of the mixture of lime and mussel shell due to the strong adsorption effects of the shells. The mixture of lime and mussel shell would be recommended for stabilizing MSS prior to being landfilled. However, longer term and larger scale investigation may be needed to better evaluate the applicability of lime and mussel shell for reducing the hazards and facilitating the management of MSS.

Contamination patterns and attenuation of pharmaceuticals in a temporary Mediterranean river

The contamination patterns and fate of pharmaceutically active compounds (PhACs) were investigated in the Evrotas River (Southern Greece). This is a temporary river with differing levels of water stress and water quality impairment in a number of its reaches. Three sampling campaigns were conducted in order to capture different levels of water stress and water quality. Four sampling sites located on the main channel of the Evrotas River were sampled in July 2015 (moderate stream flow), and June and September 2016 (low stream flow). Discharge of urban wastewater has been determined as the main source of pollution, with PhACs, nutrients and other physicochemical parameters considerably increasing downstream the wastewater treatment plant (WWTP) of Sparta city. Due to the pronounced hydrological variation of the Evrotas River, generally, the highest concentrations of PhACs have been detected during low flow conditions. Simultaneously, low flow resulted in an increased water travel time and consequently longer residence time that accounted for the higher attenuation of most PhACs. The average decrease in total concentration of PhACs within the studied waterbody segment (downstream of Sparta city) increased from 22% in July 2015 to 25% in June 2016 and 77% in September 2016. The PhACs with the highest average concentration decrease throughout the sampling campaigns were hydrochlorothiazide, followed by sotalol, carbamazepine, valsartan, and naproxen.

Investigation of pharmaceutically active compounds in an urban receiving water: Occurrence, fate and environmental risk assessment

Pharmaceutically active compounds (PhACs) recently have been recognized to constitute a health risk for aquatic ecosystems. The major pathways of PhACs to enter the aquatic environment are excretion and discharge of effluents through sewage treatment plants (STPs). The occurrence, bioaccumulation and risk assessment of lipophilic PhACs, including erythromycin, ketoconazole, indomethacin, diclofenac, gemfibrozil, bezafibrate, propranolol, carbamazepine, sertraline and 17α-ethinylestradiol were investigated in a river that receives effluents from STP. The results indicate that the PhACs were extensively existed in fish, sediment, suspended particulate matter (SPM), colloidal phase (5 kDa to 1 µm) and truly dissolved phase (< 5 kDa) water, with total concentration of ten PhACs (Σ10PhACs) of ND-19.6 ng/g, 7.3–11.2 ng/g, 25.3–101.5 ng/g, 10.1–27.7 ng/L and 67.0–107.6 ng/L, respectively. The Σ10PhACs for particulate and water samples collected from STP's outfall site were higher than those collected from upstream and downstream, indicating that the STP is an important PhACs source of river. However, the Σ10PhACs in sediment showed no significant statistical differences in the sampling area, and which was 3.5–9.5 times lower than those in SPM samples. The colloidal phase contributed 2.5–28.5% of erythromycin, 5.8–45.6% of ketoconazole, 8.4–32.2% of indomethacin, 7.0–21.4% of diclofenac, 11.6–36.9% of gemfibrozil, 10.2–45.9% of bezafibrate, 5.9–16.8% of propranolol, 1.9–11.1% of carbamazepine and 1.1–23.8% of sertraline in the aquatic environment. This suggests that aquatic particulates (e.g., colloids and SPM) maybe an important carrier for PhACs in the aquatic system. In general, the Σ10PhACs in the tissues of fish were in order as follows: kidney > brain > liver > gill > muscle. Based on truly dissolved concentrations of PhACs in the water, bioaccumulation factors were between 3.7 and 2727.3 in the fish tissues, sertraline exhibited bioaccumulation potential. In all the risk assessments, erythromycin could cause most harmful adverse health effects for the most sensitive algae group based on the acute and chronic data. In addition, the risk quotient values for diclofenac toward fish were higher than 1. These results indicate that the PhACs pose a potential risk to the aquatic organisms, especially for chronic risk.