A simple HPLC-UV method was developed and validated for the analysis of lysergol, its stress degradation profile, and pH stability studies. Instrument used was Agilent HPLC 1120 autosampler with a C18 reversed phase column and a mobile phase comprised of methanol and water (1% acetic acid) with isocratic flow. Flow rate was kept constant at 1 mL/min throughout the experiment and elution was monitored at 225 nm using UV detector. The calibration plot showed good linear relationship with r 2 = 0.9997. A well resolved single peak was observed at retention time 2.577 minute with asymmetry <1.0. The limit of detection (1.3 µg/mL), limit of quantitation (4.0 µg/mL), RSD (%) (<2.0) and capacity factor (k' = 1.577) were found to be within the acceptable limits. The stress degradation studies revealed lysergol recovery of 99.0% (wet heat induced), 65.9% (base induced), 63.7% (acid induced), 69.7% (H 2 O 2 induced), and 41.7% (UV light induced); whereas, complete drug degradation occurred at dry heat and photochemical induced degradation. From the pH rate profile studies, pH of maximum stability for lysergol was found to be 2.0.