Food Pest Research Articles

A case of recurrent convulsions caused by acute sulfuryl fluoride gas poisoning

Sulfuryl fluoride is a kind of pesticide with strong permeability, convenient use at low temperature, non-corrosive and other characteristics, which can kill food pests and has strong lethality to termites. In acute sulfuryl fluoride poisoning, patients can see recurrent convulsions, epileptic electroencephalogram abnormalities such as matrix spikes or high amplitude spikes. In this paper, a patient with sulfuryl fluoride poisoning with convulsion-based mental system symptoms was reported, and after clinical treatment with dexamethasone and phenobarbital sodium, the patient was cured and discharged.

Physicochemical and repellent properties of chitosan films loaded with essential oils for producing an active packaging effective against the food pest Sitophilus oryzae

Sitophilus oryzae (L.) (Coleoptera: Dryophtoridae) represents one of the most important pests of stored products. The adults are considered very effective packaging penetrators, causing serious economic losses. Hence, the development of new food packaging technologies is crucial to prevent its infestations. In this study, Citrus × aurantium L., Elettaria cardamomum (L.) Maton, Ocimum basilicum L., and Piper nigrum L. essential oils (EOs) were analyzed by GC-EIMS and then used to produce aromatized chitosan (CHT)-based films. The spontaneous volatile emissions of these films were evaluated by headspace solid phase micro-extraction (HS-SPME) to assess the chemical composition of the vapors released from the CHT matrix after 2, 5, 7, 9, 12, and 14 days. The thickness, moisture content, swelling properties, total soluble matter, water vapor transmission rate, color, and transmission and transparency of light of all the CHT films after 1, 7, 14, 21, 28, and 35 days were also assessed. Furthermore, the repellency of the EO-loaded CHT films was tested daily against S. oryzae adults for 28 days.Overall, adding the different EOs to the CHT films had a good impact on their physical and chemical properties. In particular, the C. × aurantium EO-loaded CHT film was characterized by the lowest swelling ratio, solubility, and water vapor transmission rate, besides having the lowest impact on the film transparency and opacity among the four EOs involved and a pleasant, lemon-like odor. The same C. × aurantium EO-loaded film was also the most effective as a repellent against S. oryzae, with a long-lasting effect for up to 28 days on the pest and a median repellency time (RT50) of 7.184 days.

Features description for the predatory mite Phytoseiulus persimilis Athias-Henriot, 1957 (Mesostigmata: subfam. Amblyseiinae)

The predatory mite Phytoseiulus persimilis A.-H. is used as aspecific acariphage on vegetable and flower crops. In protected ground P.persimilis is the most effective predator of twospotted spider mites (Tetranychidae). The scopes of pesticides use in the struggle withtwospotted spider mites – pests of food and flower crops – are limited bythe acaricides quantity. There are resistant pest races from which it ispossible to protect crops only with help of predaceous mites. The specialfeature of the P. persimilis use on protected ground is the rapid rate ofpopulation reproduction of the. The basis for the effective use of P. persimilis is the timely detection of twospotted spider mite foci sites andthe settlement of a certain number of predaceous mites there. On thebackdrop of the pesticides use, it is necessary to do surveys every 4–5days, at a temperature of 25 °C. This mite is widely used to protect gardenstrawberries, especially during tunnel cultivation. Flower crops (roses canbe taken as the example) are also effectively protected by phytoseiulus onshrub-shaped plants. However, flower plants under forcing treatmentrequire more drought-resistant acariphages.

Biology of Spodoptera litura on natural and artificial diet under laboratory conditions

Armyworm, Spodoptera litura (Lepidoptera; Noctuidae) is one of the most economically significant insect pests in global agriculture. The current study was performed to study the biology of S. litura under laboratory conditions. In the current study, the biological parameters of pests i.e., egg, larva, pupa and adult along with the duration were determined. The mean incubation period of pests on cabbage, maize and artificial diet was 2.86±0.33, 3.09 ± 0.12 and 3.97±0.77 days, respectively. S. litura had five instars. The mean developmental period of the first, second, third, fourth, fifth and sixth larval instar on cabbage was 3.44±0.11, 2.09±0.12, 4.11±0.12, 3.00±0.20, 6.68±0.23 and 5.94±0.18 days, respectively while 2.81±0.77, 3.55±0.33, 3.64±0.45, 4.43±0.71, 6.55±0.21 and 5.98±0.19 days on the maize, respectively. The mean developmental periods were 3.85±0.54, 3.45±0.63, 3.67±0.67, 4.37±0.82, 6.55±0.23 and 5.30±0.38 days of first, second, third, fourth, fifth and sixth larval instars on artificial diet, respectively. The mean total life period of S. litura was 33.43±5.86 days on cabbage and 34.79±6.95 days on maize while 35.98±7.86 days on an artificial diet. The longest developmental period of S. litura was recorded on the artificial diet while the minimum was on cabbage. The mean developmental period of pupa was 7.50±0.71 days on cabbage, 9.87±0.94 days on maize and 11.63±0.99 days on an artificial diet. Females were short-lived as compared to males. The pre-oviposition, oviposition and post-ovipositional period of S. litura on cabbage were recorded as a minimum while maximum on an artificial diet. The maximum number of eggs laid by females with the highest hatchability on cabbage followed by maize and artificial diet. An artificial diet was not good for pest development and growth as compared to a natural diet i.e., cabbage and maize. The findings will provide basic information about food pests which help in pest management.

Antimicrobial Nanomaterials Based on Halloysite Clay Mineral: Research Advances and Outlook.

Bacterial infections represent one of the major causes of mortality worldwide. Therefore, over the years, several nanomaterials with antibacterial properties have been developed. In this context, clay minerals, because of their intrinsic properties, have been efficiently used as antimicrobial agents since ancient times. Halloysite nanotubes are one of the emerging nanomaterials that have found application as antimicrobial agents in several fields. In this review, we summarize some examples of the use of pristine and modified halloysite nanotubes as antimicrobial agents, scaffolds for wound healing and orthopedic implants, fillers for active food packaging, and carriers for pesticides in food pest control.

Ecological Chemistry of Pest Control in Push-Pull Intercropping Systems: What We Know, and Where to Go?

Push-pull technology (PPT) employs mixed cropping for sustainable intensification: an intercrop repels or suppresses pests of the focal crop (push), while a trap crop attracts pests out of the field (pull), where they may be targeted for control. Underlying chemical-ecological mechanisms have been demonstrated in controlled settings, primarily for some of the best-established cereal PPT systems developed in east Africa. Yet, many questions remain regarding mechanisms, and strategies to adapt PPT for different crops and locations. We conducted a systematic review of scientific literature on PPT and related practices for biological control of pests of food and fodder. Of 3335 results, we identified 45 reporting on chemistry of trap- or intercropping systems for pest control, of which 30 focused on cereals or African pests. Seven of these reported primary chemical data: measurements from glasshouse and laboratory studies (5), or of field-collected samples (2). From these 30, we provide a database of compounds, discussing degrees of evidence for their mediation of push-pull. We depict hypothesized spatial distributions of selected compounds in PPT fields from physical properties and emission/exudation rates, and design of the east African cereal PPT system, and discuss influences on activity in field settings likely to affect success.

Sustainable Management Strategies and Biological Control in Apple Orchards

Sustainable horticultural practices address the global issues of food security, pest and disease management, soil health, water pollution, depletion of biodiversity, etc. with environment–friendly approaches. Increasingly, the adoption of such strategies is benefitting agricultural production including that in orchards. Even though several Integrated Pest Management (IPM), disease, and weed management strategies have been in use for the control of pests, diseases, and weeds in apple orchards, they are still not the most favored methods of control. There are various economic and acceptance concerns regarding their use, particularly in developing nations. A more sustainable system for apple orchards management, thus, should be adopted. 
 Here, we review various management methodologies, including the sustainable biocontrol methods, employed in the apple orchards. Reviewing these methods, we draw attention towards integrating sustainable IPM methodologies with biocontrol strategies like the use of pest-resistant cultivars, employing natural parasites and enemies of apple pests, use of agro-based pesticides, integration of technological advances that can provide real-time data to farmers and orchard scouting leading to the development of sustainable management of apple orchards. Such systems will not only reduce dependence on chemical control methods but will also minimize ecotoxicity. We also draw parallels from the biocontrol methods adopted in sustainable agri-production in other fruit orchards to suggest strategies that can be employed for sustainable apple production.

Identification of Suitable Sites for Jatropha curcas L. Bioenergy Plantation Using the AquaCrop Model

Jatropha curcas (JC), as a biofuel plant, has been reported to have various desired characteristics such as high oil content seeds (27–40%), fast-growth, easy cultivation, drought tolerance, and can be grown on marginal soil and wasteland, requiring fewer nutrients and management and does not interfere with existing food crops, insects, and pest resistance. This investigation was the first study of its type to use climatological data, blue/green water footprints, and JC seed production to identify suitable sites for JC bioenergy plantation using the AquaCrop FAO model across the Khyber Pakhtunkhwa province in northwest Pakistan. The JC seed yield (10 ton/ha) was at a maximum in the districts of Bannu, Karak, Hangu, Kurram, North Waziristan, Lakki Marwat, South Waziristan, and Dera Ismail Khan, in addition to its frontier regions, Tank, Peshawar, Mohmand, Orakzai, Khyber, Kohat, Charsadda, Mardan, Swabi, and Nowshera, respectively. Green water footprint (264 m3/ton of JC seed) and blue water footprint (825 m3/ton) was less in these areas. Furthermore, the results revealed that, depending on climatological circumstances, the southern part of the Khyber Pakhtunkhwa province is more appropriate for JC bioenergy plantation than the northern region. The districts of Bannu, Karak, Hangu, Kurram, North Waziristan, Lakki Marwat, South Waziristan, Dera Ismail Khan, and its frontier regions, Tank, Peshawar, Mohmand, Orakzai, Khyber, and Kohat, in Khyber Pakhtunkhwa province were identified to be the most ideal places for JC bioenergy plantation. As a result, under the Billion Tree Afforestation Project (BTAP) and the Green Pakistan Project, the Forest Department of Khyber Pakhtunkhwa should consider planting JC species in the province’s southern region. Furthermore, this research will provide scientific information to government and private sector officials for better management and optimum yield of the JC biofuel crop, as well as for the promotion of energy forestry in Pakistan.

Study on the Dynamic Difference between Single and Mixed Residues of Three Neonicotinoids in Brassica chinensis L.

Multiple insecticides’ residues after the mixed application of several neonicotinoids cause combined pollution and bring new challenges to food safety and pest control during agricultural production. In this study, three neonicotinoid insecticides, namely imidacloprid (IMI), acetamiprid (ACE), and thiamethoxam (TMX), were mixed and evenly sprayed on Brassica chinensis L. in the field. Then, the insecticides’ residues were dynamically monitored to determine the differences in their rates of dissipation and final residues after 10 days. The results showed that the dissipation kinetics of neonicotinoids still conformed to the first-order kinetic model for binary or ternary application of neonicotinoid mixtures, with all determination coefficients (R2) being above 0.9 and the dissipation half-life (DT50) being 2.87–6.74 d. For treatment groups with five times the recommended dosages (IMI 300 g·hm−2, ACE 900 g·hm−2, and TMX 600 g·hm−2), mixed insecticides had a slower dissipation rate, and the DT50 values of mixtures were longer than those of single insecticides. Moreover, the final insecticide residues with mixed application were higher than those of single compounds at 10 d after spraying. Thus, mixed applications of neonicotinoids may increase food safety risks as they increase the final insecticide residues in Brassica chinensis L., and care should therefore be taken when considering the combined use of such compounds.

Tribolium castaneum: A Model Insect for Fundamental and Applied Research

Tribolium castaneum has a long history as a model species in many distinct subject areas, but improved connections among the genetics, genomics, behavioral, ecological, and pest management fields are needed to fully realize this species' potential as a model. Tribolium castaneum was the first beetle whose genome was sequenced, and a new genome assembly and enhanced annotation, combined with readily available genomic research tools, have facilitated its increased use in a wide range of functional genomics research. Research into T. castaneum's sensory systems, response to pheromones and kairomones, and patterns of movement and landscape utilization has improved our understanding of behavioral and ecological processes. Tribolium castaneum has also been a model in the development of pest monitoring and management tactics, including evaluation of insecticide resistance mechanisms. Application of functional genomics approaches to behavioral, ecological, and pest management research is in its infancy but offers a powerful tool that can link mechanism with function and facilitate exploitation of these relationships to better manage this important food pest.

The first pest of stored rice in East Asia? - A maize/rice weevil impression from the Tian Lu Shan site in China

In 2018, an impression of a maize weevil, Sitophilus zeamais MOTSCHULSKY. or a rice weevil, S. oryzae L., was discovered on a potsherd from the Tian Luo Shan site, Zhejiang Province, China. This sample is the earliest archaeological record of this insect in China and possibly the earliest pest to harm rice grains in East Asia. To investigate the history of the evolution and propagation of rice pest insects in prehistoric East Asia, this paper evaluated the impression of the insect pest on a stored product. However, it was revealed that the harmed object might not be limited to rice because of the insect's bigger body size. In the previous report written in Japanese on the discovery of the weevil impression, it was identified as S. zeamais/oryzae without showing concrete length data of archaeological and modern weevils or detail key points for identification. And the report mainly focused on the history of origin and propagation of product food pest in the world. Therefore, this paper discusses diagnostic key points for identification as maize/rice weevil, with providing these detail data again.

Impact of plasma irradiation on Tribolium castaneum

Radio frequency plasma (RFP) could provide reliable, compact, cost-effective irradiation applications against insect pests of stored food and feed products. Sensitivity of red flour beetle Tribolium castaneum to RFP has been investigated using an irradiation applicator system with the two types of inert gases, argon (Ar) and helium (He), at 100 W for five exposure time levels of 0, 20, 40, 60 and 90 s, respectively. We demonstrated that He RFP was more efficient against T. castaneum than Ar RFP. In addition, a positive correlation was observed between mortality percentages of treated insect stages and exposure times for both He and Ar RFP. The adult stage showed the highest tolerance to RFP irradiation followed by larvae and pupae; however, it was more susceptible than larvae within 24 h after He RFP treatments. The optimum exposure time was 90 s with He RFP, where a full mortality at all tested stages was accomplished, while mortalities of 71.4, 65.3 and 36.7% were recorded for pupae, larvae and adult stage, respectively, after an Ar RFP treatment of 90 s. In case of treated adults, the reproduction rate was higher than treated larvae and pupae. Our findings indicated that He RFP was an effective method for inhibiting T. castaneum development and impacting the insect life cycle and could be considered a promising tool for pest control of stored food.

Destruction of food stocks by insects of the fireworm family: hygienic aspects and preventive measures (review)

A review of the literature on the issue of damage to food stocks by insects of Pyralidae. Pyralidae — the most dangerous and widespread pests of food stocks, significantly affecting the sanitary and hygienic condition and quality of products of bakeries, bakeries, confectionery factories, flour and cereals and other food enterprises, warehouses and stores. They damage not only grain, flour and products from them, but also all types of dried fruits, dried vegetables, nuts, soy and chocolate candies, as well as various seeds, medicinal raw materials and herbariums, feed, cake, bran, tobacco, spices, coffee beans, cocoa beans, cork. Variants of damage caused by these insects to food stocks are described. Methods of prevention of food supply contamination and pest control are analyzed and systematized.

Morphophysiological analysis of the salivary glands of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) exposed to ozonated water: A control strategy.

The tick Rhipicephalus sanguineus sensu lato has great medical and veterinary importance, mainly because the ability to transmit many diseases, causing harm to pets but also risks to public health. The blood spoliation and transmission of pathogens occur because of the immunosuppressive action of these ticks' saliva, a potent mixture of bioactive substances that is secreted by the salivary glands, one of the organs responsible for their biological success, and hence the target of studies for their control. Ozone has promise for use as an alternative acaricide, due to its proven efficiency in controlling agricultural and food pests, besides posing no risk of environmental contamination or to animal and human health. Therefore, this study evaluated the acaricidal potential of exposure of females of R. sanguineus s.l. to ozonated water at many concentrations and analysed the morphophysiological alterations of the salivary glands, employing histological and light microscopic techniques. The results demonstrated that the ozonated water at the concentrations investigated caused severe alterations in the salivary glands, bringing a new perspective for control of R. sanguineus s.l., through an ecologically correct method due to the absence of harm to non-target organisms and the environment.

Development of encapsulated peppermint essential oil in chitosan nanoparticles: characterization and biological efficacy against stored-grain pest control

Peppermint oil (PO) has widely been reported for their nutritional and biomedical properties. Essential oil (EO) has several properties, usage restricted due to environmental condition, polymer based nanoencapsulation of EOs is developed as one of the promising technique to address this limitation. In this current study emphasizes on developing Peppermint oil (PO) encapsulated chitosan nanoparticles (CS NPs) by ionic gelation method in order to improving its insecticidal potential effective management of two stored grain pest. The nano-encapsulated PO (CS/PO NPs) was confirmed by UV–Vis spectrophotometry and X-ray diffraction (XRD) techniques. The physicochemical characterization of CS/PO NPs revealed <563.3 nm size, Zeta potential of −12.12 mV, encapsulation efficiency >64% and loading capacity >12.31% respectively. Developed CS/PO NPs demonstrated the toxicity studies have significantly efficacy against both stored product pest compared to control. In-vivo percent inhibition of AChE activity ranged between S. oryzae (52.43% and 37.71% and and T. castaneum (37.80% and 31.29). Overall, these results highlighted that polymer chitosan nanoparticle encapsulated with essential oil is a promising novel design that could be promoted in stored food pest management schedule for T. castaneum and S. oryzae.

Ruta chalepensis L. Essential Oil Has a Biological Potential for a Natural Fight against the Pest of Stored Foodstuffs: Tribolium castaneum Herbst

Chemical pesticides used against insect pests of stored food have adverse effects on both health and the environment. So, the present study aims to evaluate the insect repulsive and insecticidal power of Ruta chalepensis L. essential oil (EO) from the region of Oulmes (Central plateau of Morocco); the ultimate objective is to develop a biological and ecological control strategy against pests. Thus, the EO obtained by hydrodistillation from the aerial parts of Ruta chalepensis L. was identified by GC-MS; its repellent and fumigant toxicity effects on adults of Tribolium castaneum Herbst were, respectively, investigated by the preferential area method on a filter paper and the inhalation test. The insecticide power was estimated by determining the percentage of mortality as a function of the duration of exposure and concentration of the EO. The essential oil obtained is characterized by the dominance of 2-undecanone (64.35%), piperonyl piperazine (11.9%), 2-decanaone (5.12%), 2-dodecanone (4.52%), decipidone (3.9%,) and 2-tridecanone (2.36%). This EO is endowed with a very repulsive power belonging to class V, which is strongly due to its majority compound 2-undecanone. The dose 0.038 μl/ml gave a repellent power of 100% after 15 min. The tests also revealed a considerable insecticidal effect, which reached 100% after 48 hours at a dose of 0.62 μl/ml. The calculation of the lethal dose causing 50% mortality (LD50) and the lethal times after which there is 50% mortality (LT50) allowed deducing that the insecticidal effect of Ruta chalepensis L. is time- and dose-dependent. Hence, the effectiveness of Ruta chalepensis L. EO attests that it can constitute a healthy alternative to fight against Tribolium castaneum Herbst.

Solasodine phytocompound controlled the food pest Tribolium castaneum growth

Chemical and non-chemical methods are widely employed for managing storage food pest red flour beetle, Tribolium castaneum. This study validated the solasodine effect on the larval growth and other life cycle intermediates of the flour beetle. The target organism was reared on mixed flour of wheat and jowar and treated with different concentrations of solasodine preparations on the fifth instar larvae. The survival rate of the treated organism was statistically evaluated. The concentration of 0.25 ug/mL solasodine and above significantly inhibited the larval growth and produced deformed mosaics, pupae, and adults. Unlike synthetic chemical pesticides, solasodine that was reported to contribute to health benefits in humans controlled the Tribolium castaneum growth in reared media.

Food web structure of parasitoids in greenhouses is affected by surrounding landscape at different spatial scales

Landscape management affects species interactions and can have notable effects on food web structure. Local parasitoid populations in greenhouses usually migrate from outside crops; biological control of greenhouse aphids may be thus highly dependent on the composition of surrounding landscape. However, it is less clear how surrounding landscape composition affects primary-hyperparasitoid food webs and pest control services in greenhouses. We investigated the food web of parasitoids on melon-cotton aphid (Aphis gossypii Glover) in watermelon greenhouses in two suburban Beijing counties over two years. We used the quantitative food web metrics (generality, vulnerability, link density, and interaction evenness) to assess the effects of landscape composition on primary-hyperparasitoid food web structure. We found that landscape with more cropland within 1–3 km tended to have more primary parasitoids per hyperparasitoid species (generality). Higher proportions of woodland at the 0.5 km scale were negatively correlated with the mean numbers of hyperparasitoid per primary parasitoid species (vulnerability), as well as with hyperparasitism rate and hyperparasitoid richness. Link density, interaction evenness and aphid mortality caused by parasitoids (parasitism rate) were not affected by landscape factors. However, active primary parasitism (biocontrol potential) increased with the proportion of woodland. This suggested that the bottom-up effect induced by primary parasitoids might benefit hyperparasitoids, thus exerting little influence of primary parasitoids on pest control. The top-down effect of hyperparasitoids may reduce with increasing woodland proportion. To enhance the effects of primary parasitoids, landscape management programs should also target, and thus limit the impact of hyperparasitoids.

NIR spectroscopy vs. food pests: The case of stored rice

Rice is one of the most widely consumed cereals, and it represents a staple food for several populations all over the world. One of the main peculiarities of this food commodity is that it can be stored for relatively long periods, maintaining its nutritional properties and its organoleptic attributes. Nevertheless, it is not uncommon that pests infest granaries, altering the characteristics of cereals, making them less valuable and/or un-edible. Avoiding the presence of insects in storehouses is a task difficult to accomplish; consequently, different methods for detecting pests’ infestation in food commodities have been developed. In general, they are based on physical sensors, or they exploit analytical techniques such as high-performance liquid chromatography, nuclear magnetic resonance spectroscopy, enzyme-linked immunosorbent assays or other bio-chemical devices to detect insects’ byproducts. Despite these approaches providing accurate results, they all present the same inconvenience: they are destructive. In the light of these considerations, the present work aims at developing a non-destructive NIR-based strategy to detect the presence of Plodia interpunctella, one of the most common intruders of granaries, in rice parcels. In order to achieve this goal, 1525 samples of rice have been analysed by NIR spectroscopy and then partial least squares discriminant analysis was used to discriminate the edible grains from the infested ones. The proposed methodology provided extremely good results, properly assigning 484 test samples over 500.

Isolation by Distance, Source-Sink Population Dynamics and Dispersal Facilitation by Trade Routes: Impact on Population Genetic Structure of a Stored Grain Pest.

Population genetic structure of agricultural pests can be impacted not only by geographic distance and the broader ecological and physical barriers but also by patterns related to where crops are produced and how they are moved after harvest. Stored-product pests, for instance, specialize in exploiting grains such as wheat and rice from on-farm storage through transportation to final processing at often geographically distant locations; therefore human-aided movement may impact their dispersal. Although stored product insects are associated with stored grain, they can also exploit resources in the surrounding environments so different ecological regions where the grain is grown and stored may also influence population structure. Here we used 1,156 SNP markers to investigate how geographic distance, ecological and agricultural variables can impact the genetic structure and gene flow of the stored food pest beetle Rhyzopertha dominica. We found a substantial degree of admixture between weakly structured populations in the US. Ecological regions were more important in explaining R. dominica population structure than crop type, suggesting insect movement between wheat and rice grain distribution channels. We have also found a significant correlation between the genetic and geographical distance (i.e., isolation by distance). However, our modeling approach combining the ecological and management variables has highlighted the importance of the volume of grain received by a location in the dispersal dynamics of the pest. The first-generation migrant analysis offered additional supported to movement over great distances that are likely associated with grain movement. Our data suggest that a multitude of factors play small but significant parts in the movement dynamics of the pest. The beetles can take advantage of the source-sink dynamic of grain movement in the US, but also engage in a high rate of movement at the local scale. Understanding population structure for R. dominica will provide insights into the potential for local processes of adaptation and broader patterns of movement that will impact management programs and the potential for spread of resistance genes.