Peroxide Pretreatment Research Articles

Fractionation of lignin and fermentable sugars from wheat straw using an alkaline hydrogen peroxide/pentanol biphasic pretreatment

To breakthrough the delignification saturation point (DSP) of alkaline hydrogen peroxide (AHP) pretreatment, a biphasic AHP/pentanol (AHPP) pretreatment was proposed in this work. The temperature and H2O2 concentration were evaluated. Under the optimal conditions (110 °C, 2 h, 4 % H2O2), 70.73 % of lignin was removed, which was increased by 11.65 % than the traditional AHP pretreatment, indicating successful overcoming of the DSP by adding pentanol. 85.74 % and 88.62 % of glucan and xylan digestibility were achieved, respectively, which increased by 7.41 % and 5.87 % as compared to AHP pretreatment. Furthermore, the lignin extracted from the organic phase accounted for 38.51 % of the delignification, and it had a low molecular weight, effectively preserving the β-O-4 bonds. Finally, satisfied pentanol recovery (77.91 %) and delignification (57.19 %) along with excellent glucan (76.11 %) and xylan (77.52 %) digestibility were reached after fourth recycling of AHPP pretreatment. Therefore, AHPP pretreatment was a promising method for biomass valorization within biorefinery concept.

Extraction of bio-hydroxyapatite from devilfish (Loricariidae) for the fluoride and cadmium adsorption from water and its feasible photocatalytic properties

In this study, the adsorption capacity of bio-hydroxyapatite (Bio-HAp) from devilfish for the removal of F- and Cd(II) from aqueous solutions was investigated. This material was synthesized according to a 2FI factorial experimental design by varying the extraction conditions for Bio-HAp, including the type of pretreatment (alkaline and peroxide), the calcination temperature from 550 to 850 °C, and the sonication process. The maximum adsorption capacities were 8.48 and 83.56 mg g-1 for F- and Cd(II), respectively. Statistical analysis showed the importance of the type of pretreatment, temperature, and sonication for adsorption. The predicted optimal conditions were Bio-HAp extracted from bone with peroxide pretreatment, calcination at 550 °C and sonication. The surface of the Bio-HAp was found to be mesoporous and basic in character. TGA, FT-IR and SEM-EDS characterizations confirmed the presence of F- and Cd(II) on the Bio-HAp surface and confirmed the adsorption mechanisms by electrostatic forces, ion exchange, and chemisorption. The Praunitz-Rake model of adsorption isotherm showed better agreement with the equilibrium adsorption data of F- and Cd(II) at pH 7. Furthermore, photodegradation experiments showed 100% degradation methylene blue (MB) under natural sunlight. This study indicates an effective photodegradation process, suggesting high adsorption capacity of the samples. The use of devilfish as an adsorbent promises to be a viable and sustainable option for the removal of fluoride and cadmium from water, and for use in photodegradation experiments.

Innovative integration of chitosan biopolymer for felt-free woolen fabric: A synergistic approach with digital textile printing

Wool, renowned for its ancient legacy and unique attributes, is a prized textile fiber with versatile applications in various domains. However, the susceptibility to shrinkage due to the scaly structure of overlapping cells (scales) in the cuticle poses a challenge, prompting the widespread adoption of chlorination to enhance resistance to felting and shrinkage. This research explores the potential of chitosan biopolymer as an effective anti-felting agent and enhancer of wool dyeability. With its non-toxic, biocompatible, and biodegradable properties, chitosan is a promising substitute for various textile finishes. Recent innovations include chitosan’s contribution to improving the digital printing process, particularly in enhancing color strength and sharpness on cotton fabric. Identifying a research gap in exploring the synergistic potential of chitosan for anti-felting, this study introduces an alkaline hydrogen peroxide pre-treatment to enhance chitosan absorption through the pad-dry-cure method. Chitosan, acting as a binder, is strategically applied in the pre-treatment to fix pigments with reactive inks during inkjet printing. Critical process parameters, such as chitosan concentration, curing temperature, and time, were optimized for area shrinkage (%) to achieve a felt-free property for wool. Subsequent optimization focuses on color strength, print sharpness, and washing durability of digitally printed patterns. The results obtained from this research were quite promising in terms of K/S value, wettability, pilling behavior, tensile strength, edge sharpness, washing fastness, and fabric shrinkage resistance.

Promoting enzymatic hydrolysis of corncob through alkaline hydrogen peroxide pretreatment and addition of additives

Promoting enzymatic hydrolysis of corncob through alkaline hydrogen peroxide pretreatment and addition of additives

Cold plasma/alkaline pretreatment facilitates corn stalk fractionation and valorization towards zero-waste approach

The present study employed alkaline hydrogen peroxide pretreatment coupled with cold plasma for corn stalk’s complex biopolymers fractionation and valorization through enzymatic hydrolysis. This hurdle approach enables the prolonged generation of reactive oxidative species, initiating chemical reactions otherwise hardly possible under ambient conditions. A delignification rate between 76±1 and 86±2 % was obtained under different process parameters in a significantly shorter time compared to conventional alkaline pretreatment. Up to 94±4 % of cellulose in treated biomass was converted to glucose after enzymatic hydrolysis, achieving around 3.5 times higher glucose yield than the raw biomass. Different spectroscopic analyses confirmed specific alterations in the structure of the lignin-rich fraction caused by cold plasma. Micro- and nanoscale lignin particles obtained were rich in total phenolic content, reaching up to 140±20 and 107±12 µg gallic acid equivalents (GAE) per mg of lignin, respectively. The proposed concept of corn stalk fractionation through biorefinery can open new opportunities for valorizing different agricultural waste types.

Alkaline hydrogen peroxide pretreatment of bamboo residues and its influence on physiochemical properties and enzymatic digestibility for bioethanol production

Bamboo is a perennial rapid-growing plant that is given preference for renewable biosources for biofuels and bio-based chemical conversion. Bamboos are rich in cellulose and have highly recalcitrant biomass due to high lignin. Bamboo is abundantly available in Northeastern India and can be utilized as a feedstock biofuels. Here, we evaluated the pretreatment of bamboo residues Dendrocalamus strictus with different concentrations of alkali, hydrogen peroxide, and alkaline hydrogen peroxide and its influence on biomass digestibility for enhancement of sugar recovery with Celic C cellulase enzyme blend. Enzymatic hydrolysis data indicated untreated raw biomass showed a digestibility of 40% after 48 h of incubation. The biomass pretreated with alkali showed a maximum digestibility of 61% obtained from 10% loaded with 0.5% w/v NaOH. Pretreatment of the bamboo with H2O2 shows a maximum digestibility of 75% from biomass loaded with 1% w/v of H2O2. Combinational pretreatment of alkaline hydrogen peroxide showed a maximum efficiency of biomass digestibility of 83% attained from biomass loaded with 1% w/v NaOH-H2O2. Crystallinity index (CrI) analysis showed that CrI increased from 64% to 70.75% in pretreated biomass. FTIR and SEM analysis show changes in functional groups, morphology, and surface of biomass in pretreated biomass. Compositional analysis shows that 68% of lignin removal is obtained from alkaline hydrogen peroxide pretreatment. Cellulose content increased from 52% to 65%, and hemicellulose decreased from 18.6% to 8.6%. Results indicated that the potential possibility of bamboo waste biomass as feedstock for biorefinery products and alkaline hydrogen peroxide pretreatment methods is an efficient strategy for sugar recovery for bioethanol production.

Adequately converting poplar sawdust’s carbohydrates to furfural and glucose by employing acid oxidation pretreatment and solid acid catalysis

The utilization of lignocellulosic biorefinery residue has always been a major obstacle hindering its industrialization. In this work, the acetic acid assisted hydrogen peroxide pretreatment (AHP) and enzymatic hydrolysis was employed to facilitate the efficient bioconversion of poplar sawdust (PS), while simultaneously utilizing the solid acid catalyst (SA) synthesized from enzymatically hydrolyzed PS (EPS) for furfural production from prehydrolysate. It demonstrated that enzymatic hydrolysis of PS achieved a glucose yield of 61.3 %, which can be attributed to the effective removal of xylan and lignin through AHP pretreatment. The structural characterization revealed that SA-EPS exhibited a higher pore density, more acidic sites, and an increased presence of acidic groups compared with SA prepared from PS. Ultimately, the utilization of SA-EPS significantly augmented furfural yield and selectivity to 54.3 % and 64.1 %, respectively. By successfully synthesizing SA using lignin-rich EPS, the value-added benefits and increased efficiency have been achieved in biorefinery processes.

Synergistic enhancement of nitrogenous compounds in bio-oils: Hydrogen peroxide pretreatment and nitrogen-rich pyrolysis

A novel pretreatment method, combining H2O2 and nitrogen-rich pyrolysis for biomass was proposed to address the issue of low nitrogen compounds or levoglucosan content in pyrolysis bio-oil. Bio-oil enriched with high content of levoglucosan and nitrogenous compounds was obtained. In this study, the effects of pretreatment conditions (H2O2 solution concentration, pretreatment time, solution pH) and nitrogen-rich ratio on the pyrolysis characteristics of corn stover were investigated. The difficulty of converting different components into nitrogenous compounds was evaluated for the first time. The results reveal that H2O2 pretreatment effectively removed most lignin and ash, leading to a substantial enrichment of cellulose. Notably, carbohydrate content in bio-oil increased by more than 970 %. However, excessive pyrolysis temperature and pH of H2O2 solution can aggravate the decomposition of carbohydrates. The co-pyrolysis of the pretreated corn stover with urea increased the bio-oil yield (up to 56.32 wt%) and the content of nitrogenous compounds (43.16 wt%). The order of ease in converting certain components into nitrogenous compounds is Ketones > Aldehyde > Furans > Phenols > Esters > Alcohols. This study not only clarified the correlation coupling mechanisms between raw material pretreatment conditions and nitrogen-rich ratio with pyrolysis product characteristics. It can also provide guidance for biorefining. Based on the excellent lignin removal effect of the method proposed in this study, it has broad application prospects in the field of biorefinery of lignocellulosic biomass.

Physiological and molecular analysis of pitaya (Hylocereus polyrhizus) reveal up-regulation of secondary metabolites, nitric oxide, antioxidant defense system, and expression of responsive genes under low-temperature stress by the pre-treatment of hydrogen peroxide

Low-temperature events are one of the leading environmental cues that considerably reduce plant growth and shift species biodiversity. Hydrogen peroxide (H2O2) is a signaling molecule that has a distinguished role during unfavorable conditions and shows outstanding perspectives in low-temperature stress. Herein, we elucidated the protective role and regulatory mechanism of H2O2 in alleviating the deleterious effects of low-temperature stress in pitaya plants. Micropropagated pitaya plants were cultured in Murashige and Skoog media supplemented with different levels of H2O2 (0, 5, 10, and 20 mM) and then exposed to low-temperature stress (5 °C for 24 h). H2O2 at 10 mM, improved low-temperature stress tolerance by relieving oxidative injuries and ameliorating growth parameters in terms of fresh weight (66.7%), plant length (16.7%), and pigments content viz., chlorophyll a (157.4%), chlorophyll b (209.1%), and carotenoids (225.9%). H2O2 counteracted the low-temperature stress by increasing amino acids (224.7%), soluble proteins (190.5%), and sugars (126.6%). Simultaneously, secondary metabolites like ascorbic acid (ASA), anthocyanins, phenolics, flavonoids, total antioxidant (TOA), and proline were also up-regulated by H2O2 (104.9%, 128.8%, 166.3%, 141.4%, and 436.4%, respectively). These results corresponded to the stimulative role triggered by H2O2 in boosting the activities of catalase (22.4%), ascorbate peroxidase (20.7%), superoxide dismutase (88.4%), polyphenol oxidase (60.7%), soluble peroxidase (23.8%), and phenylalanine ammonia-lyase (57.1%) as well as the expression level of HpCAT, HpAPX, HpSOD, HpPPO, and HpPAL genes, which may help to moderate low-temperature stress. In conclusion, our findings stipulate new insights into the mechanisms by which H2O2 regulates low-temperature stress tolerance in pitaya plants.

Iron ion catalyzed hydrogen peroxide pretreatment for bio‐jet fuel production from straw biomass

AbstractBACKGROUNDThe development of efficient and green pretreatment pathway is of great significance for promoting biofuel production from lignocellulosic waste. The purpose of this work was to evaluate that iron ion‐catalyzed hydrogen peroxide pretreatment can be used as an environmentally friendly pretreatment way for bio‐jet fuel production using straw.RESULTSThe effects of iron ion‐catalyzed hydrogen peroxide pretreatment (Fe‐HP) on the enzymatic hydrolysis, fermentation and bio‐jet fuel production using straw were investigated. It was found that the iron ion‐catalyzed hydrogen peroxide pretreatment significantly improved the enzymatic hydrolysis and fermentation efficiency of straw biomass. Subsequently, the directional catalytic transformation of straw‐derived fermentation broth to bio‐jet fuels was achieved using a dual functional catalyst (HSAPO34/NiHBET). High conversion (94.9%) and good jet‐fuel selectivity (77.3%) were obtained in the conversion process of straw. On account of the biomass characterization and the analysis of reactive oxygen species during the Fe‐HP pretreatment, the role and mechanism of the Fe‐HP pretreatment was addressed.CONCLUSIONThe Fe‐HP pretreatment can be used as an environmentally friendly pretreatment way for jet fuel production from straw biomass. Fe‐HP pretreatment increased sugar yield and sugar concentration during enzymatic hydrolysis, which was beneficial for subsequent fermentation and bio‐jet fuel production. The reactive oxygen species take a prominent role in the Fe‐HP pretreatment of lignocellulose. © 2024 Society of Chemical Industry (SCI).

Investigation of the alkaline hydrogen peroxide pretreatment: From cellulose saccharification to lignin isolation

Alkaline hydrogen peroxide (AHP) pretreatment, a green and effective technology for biomass fractionation, was studied to overcome the recalcitrance of bamboo for lignin isolation and facilitating enzymatic saccharification. In order to explore the impact of AHP pretreatment on the cellulose saccharification of bamboo, the glucan/xylan recovery, lignin removal, and structural properties of solid substrate were investigated in this research. AHP pretreatment at 90 ℃ for 60 min with 3.0 % H2O2 resulted in the maximum glucan/xylan hydrolysis yield reaching 90.62 % and 88.30 %, respectively. Specially, for the first time, the recovered lignin from the AHP liquid possesses up to 59.84/100Ar of β-O-4 linkages, abundant ferulic acid (FA), and p-coumarate (pCE) structures, as well as a homogeneous molecular structure with high purity (84.04%-96.20%) and preferable antioxidant activities (IC50: 0.015–0.018 mg/mL). These results provided theoretical support for the comprehensive valorization of lignocellulose, which is favorable for achieving viable biorefineries.

Recovery of p-Hydroxybenzoic Acid from Cu-Catalyzed Alkaline Hydrogen Peroxide Pretreatment of Hybrid Poplar

Recovery of <i>p</i>-Hydroxybenzoic Acid from Cu-Catalyzed Alkaline Hydrogen Peroxide Pretreatment of Hybrid Poplar

Efficacy of alkaline peroxide pretreatment on Eucalyptus grandis as effective lignin removal strategy for production of ethanol using enzymatic hydrolysis

Efficacy of alkaline peroxide pretreatment on Eucalyptus grandis as effective lignin removal strategy for production of ethanol using enzymatic hydrolysis

CHARACTERIZATION OF LIGNIN DISSOLVED DURING ALKALINE SODIUM BOROHYDRIDE AND HYDROGEN PEROXIDE PRETREATMENTS OF SUGAR MAPLE (ACER SACCHARUM)

Sugar maple (Acer saccharum) wood chips were pretreated with aqueous sodium hydroxide, sodium borohydride, and hydrogen peroxide pretreatments, and the characterization (i.e., amount, molar masses, and molar mass distributions) of the lignin dissolved during the pretreatments was performed. Of the applied pretreatments, especially borohydride pretreatments were efficient in dissolving lignin from this feedstock, as 20% to 28% of the dissolved organics were found to consist of dissolved lignin, contributing to 3.4% to 9.0% of the initial wood lignin. Corresponding values for 2.5% and 5.0% alkaline peroxide pretreatments were from 5.9% to 9.2% dissolved lignin of the total dissolved organics and from 1.1% to 3.2% of the initial wood lignin. Additionally, determined molar masses and molar mass distributions indicated that borohydride pretreatments generally led to the formation of smaller-molar-mass lignin fragments when compared to reference pretreatments and to pretreatments conducted with alkaline peroxide process, indicating more efficient lignin degradation caused by borohydride addition. For example, Mn and Mw values of the analyzed reference effluents were 54%-62% and 66%-71% higher when compared to the corresponding borohydride effluents, respectively.

Degrading surface-water-based natural organic matter and mitigating haloacetonitrile formation during chlorination: Comparison of UV/persulfate and UV/hydrogen peroxide pre-treatments

Haloacetonitriles (HANs) are unregulated disinfection by-products that are more toxic than regulated species. Therefore, efficient decomposition of HAN precursors prior to disinfection is crucial for allaying the potential HAN-induced health risks. This study investigated the key roles of ultraviolet-activated persulfate (UV/PS) treatment in alleviating HAN formation. The effects of UV/PS treatment were evaluated by correlating with the characteristics of organic matter in surface water and comparing with conventional UV/H2O2 treatment. Upon irradiating raw water samples and a Suwannee River humic acid solution spiked with 10 mM PS or H2O2 with 254 nm UV light, UV/PS treatment was found to be more potent than UV/H2O2 in mitigating the HAN production and degrading organic substances; moreover, UV/PS treatment effectively decreased the dissolved organic nitrogen (DON) content. In contrast, UV/H2O2 treatment did not induce any noticeable reduction in DON level. Furthermore, both UV/PS and UV/H2O2 treatments reduced the dichloroacetonitrile (DCAN) formation potential (FP), leading to strong correlations with the degradation of aromatic and humic-acid-like compounds. Notably, UV/PS treatment efficiently decreased the FP of bromochloroacetonitrile (BCAN) and dramatically reduced that of dibromoacetonitrile (DBAN) after a sharp increase; however, UV/H2O2 treatment gradually increased the DBAN-FP. Bromide was activated by sulfate radicals during UV/PS treatment, negatively correlating with the BCAN-FP and DBAN-FP, indicating that the formation of reactive bromine species increased the DBAN-FP; however, excessive oxidation possibly led to the recovery of inorganic bromine for decreasing the BCAN-FP and DBAN-FP. Additionally, UV/PS treatment effectively suppressed toxicity owing to its high reduction rate for brominated HANs; in contrast, UV/H2O2 treatment resulted in less significant BCAN and DBAN reductions, leading to minimal net reduction in toxicity. Overall, UV/PS treatment was remarkably effective at diminishing the toxicity of brominated HANs, underscoring its potential to mitigate drinking-water-related health risks.

The effects of sodium persulfate pretreatment and calcium peroxide pretreatment on digested sludge and raw sludge dewaterablity

Comparison between the dewatering performance of raw sludge and digested sludge is lacking. In this study, pretreatment with sodiumpersulphate (SP), calcium peroxide (CP) for improving the dewaterability of raw sludge (RS), digested sludge (DS), and sludge digested for 3 days (DS-3) were investigated. Using ferrous sulfate as a catalyst to produce sulfate radicals from SP. Results suggested that the maximum increment of free moisture of 4.69 % was achieved for the DS-3 of pretreatment with SP, and the mechanism investigation revealed that two kinds of pretreatments could enhance sludge floc disintegration, degrade the protein (PN) fraction of extracellular polymeric substances (EPS), and accelerate the release of mechanically bound moisture. Compared with RS, the organic matter content of DS and DS-3 sludge samples pretreated by SP and CP was lower, viscosity was decreased, zeta potential was closer to zero, and the strength of amino, amide II and other hydrophilic functional groups decreased or disappeared.

Detoxification and enhancement of in vitro rumen digestibility of exhausted olive pomace wastes through alkaline hydrogen peroxide treatment

Abstract Background Due to the sharp rise in animal feed costs, funding alternatives to substitute high-cost raw materials used in animal feed is a persistent need. This study investigated the effect of alkaline hydrogen peroxide pretreatment as straightforward non-toxic technology to enhance the in vitro rumen digestibility of exhausted olive pomace (EOP), an abundant agricultural waste, to be suitable as animal feedstock. It examined the efficiency to eliminate the toxic phenolic content and minimize lipid oxidation of EOP. Results The pretreatment was first optimized using a central composite experimental design. Under the optimized conditions (1.6% H2O2, 5% NaOH), the measured phenolic content was 1.51 ± 0.03 mg/100 g dry weight (DW) for treated olive pomace (TOP) versus 4.91 ± 0.06 mg/100 g for the untreated one. The pretreatment showed that approximately 25% of the lignin was removed. Crude proteins, neutral detergent fibers, and acid detergent fibers yields of TOP were, respectively, 3.320 ± 0.05, 75.24 ± 0.23, and 54.05 ± 0.35 g/100 g of DW, significantly more important than those of untreated EOP. The enzymatic hydrolysis with a cellulase-based cocktail (Celluclast15 FPU/gDW), recorded a 48% of reducing sugar yield for TOP against 33% for EOP. When the in vitro organic matter digestibility (IVOMD) was assayed, the potential gas production of TOP (41.371 ml/g DM) was significantly higher than EOP (25.771 ml/g DM). The metabolizable energy of TOP (9.28 kcal/kg DM) was higher than that of EOP (7.78 kcal/kg DM). Conclusions The present study revealed that alkaline hydrogen peroxide (AHP) could be an efficient treatment for the detoxification and enhancement of in vitro rumen digestibility of olive pomace. This straightforward approach demonstrated that treated olive pomace waste may be well valorized as suitable animal feedstock. Graphical Abstract

Anaerobic fermentation of poplar processing residue to produce methane by alkaline hydrogen peroxide (AHP) pretreatment

ABSTRACT The study evaluated the effects of alkaline hydrogen peroxide (AHP) pretreatment on poplar processing residue for methane production. The delignification of poplar processing residue was used as the basis for evaluation. The experiment considered the pretreatment time (0 h-24 h), the hydrogen peroxide (H2O2) concentration (0.5%-2.0%), and potassium hydroxide (KOH) concentration (1.0%-3.0%) at a temperature of 70°C. Subsequently, anaerobic fermentation experiments were conducted using the pretreated biomass with sludge as the inoculum under mesophilic temperature conditions (37 ± 1°C). The initial feedstock (poplar processing residue) contained 40.31 ± 0.06% cellulose, 31.17 ± 1.59% lignin, and 14.85 ± 0.38% hemicellulose. The AHP pretreatment caused changes in the lignocellulosic content of the feedstock, particularly affecting the lignin structure compared to cellulose and hemicellulose. At a pretreatment time of 18 hours, temperature of 70°C, and concentrations of 2.0% H2O2 and 1.0% KOH, the AHP pretreatment resulted in a higher methane production of 237.4 mL per gram. These findings demonstrate that AHP pretreatment is an effective strategy for enhancing methane production from poplar.

Delignifikacija kukuruznog oklaska pomoću peroksidnog predtretmana potpomognutog mikrotalasima

Lignocellulosic biomass comprises the crop residues, which remain in large quantities from various stages of crop processing. Its main constituents, cellulose, hemicellulose, and lignin, are interlinked by hydrogen and covalent bonds and form a robust and intricate matrix making it resistant to enzymatic degradation. Pretreatment is crucial in lignocellulosic biomass processing, aiming to remove lignin and enhance enzyme access to polysaccharides. This study examines how microwave-assisted peroxide pretreatment affects the delignification of corncobs, aiming to enhance the efficiency of utilizing this agricultural residue in fermentation processes. Taguchi orthogonal array was used to optimize pretreatment conditions and assess the effects of individual parameters (temperature, H 2 O 2 dose, and pretreatment time) on delignification efficiency. The results showed the significant influence of pretreatment conditions on lignin removal from corncobs, with the highest delignification achieved at an H 2 O 2 dose of 500 mg/g, temperature of 100 °C, and pretreatment time of 2 minutes. H 2 O 2 dose had the most substantial impact on the delignification, followed by temperature and pretreatment time. The observed 81.6% delignification and 61.9% increase in cellulose content are pivotal for enzymatic hydrolysis efficiency. This improvement suggests enhanced enzyme availability during hydrolysis and reversible binding to polysaccharide active sites, potentially leading to higher sugar yields.

Effect of pretreatment with hydrogen peroxide at different pHs on corn stalk: Characterizations of structure, composition, and pyrolysis properties

Appropriate pretreatment plays a pivotal role in biomass pyrolysis as it facilitates high-value utilization of pyrolysis products. In this study, hydrogen peroxide pretreatment with cleanliness, economy, and efficiency was applied for fast pyrolysis of corn stalks. The effects of pyrolysis temperature, hydrogen peroxide pretreatment, and different pHs on the structure of corn stalks and the properties of resultant pyrolysis products were investigated. The results revealed that the pretreatment could effectively promote lignin depolymerization and significantly reduce the reaction activation energy (E). Meanwhile, the pretreatment increased the cellulose content and removed the majority of alkali and alkaline earth metals from the biomass. The removal of lignin and ash by the pretreatment was affected by the pH of the hydrogen peroxide solution. Moreover, the composition of bio-oil changed significantly. Among them, the relative content of levoglucosan in the bio-oil from untreated raw material was only 1.38 %. After pretreatment, the relative content of levoglucosan was up to 14.5 %. The present findings offer valuable guidance for the further exploration and exploitation on hydrogen peroxide pretreatment for depolymerization of woody fibrous biomass as well as for the preparation of high-value chemical products.