Introduction: Anterior cruciate ligament (ACL) rupture is a sports-related knee injury that is quite often encountered. It is estimated that the annual incidence of these injuries reaches 5 to 8 per 10,000 individuals. There are various graft options for ACL reconstruction, such as hamstring tendon autograph, bone-patellar tendon-bone autograph (BPTB), and peroneus longus tendon autograft. Regeneration of the peroneus tendon can be assessed by several modalities, one of which is magnetic resonance imaging (MRI). One of the main concerns after PLT grafting is the potential for a decrease in plantar flexion strength and ankle eversion. Purpose: of this study was to assess the regeneration capabilities of the peroneus tendon after ACL reconstruction and its relation to the function of the muscle. A better understanding of this topic may provide an alternative, and possibly a better intervention to detect the presence of peroneus tendon regeneration and increase functional output in ACL reconstruction patients. Material and Methods: This study is an observational study using a case-control design. From the population of the study subjects, sampling was carried out that met the requirements of research inclusion and then data collection was carried out. An examination of the isokinetic strength of the plantar ankle flexion and eversion, then the data obtained is processed using SPSS 25. Results: Based on the results of the independent t test, it was found that the isokinetic strength of the 1st ray plantar flexion and ankle eversion is a predictor of regeneration of the peroneus longus (p < 0,05; p < 0.05). Conclusion: The isokinetic strength of the ankle plantar flexion and eversion is a predictor of regeneration of the peroneus longus tendon as a "donor site" after 1 year post ACL reconstruction. Keywords: ACL reconstruction, regeneration of peroneus longus, isokinetic strength of the ankle