Permeable Vessel Research Articles

Corrigendum to “Magnetic drug targeting during Caputo-Fabrizio fractionalized blood flow through a permeable vessel through a permeable vessel” [Microvasc. Res. (2022) 104262

Corrigendum to “Magnetic drug targeting during Caputo-Fabrizio fractionalized blood flow through a permeable vessel through a permeable vessel” [Microvasc. Res. (2022) 104262

Magnetic drug targeting during Caputo fractionalized blood flow through permeable vessel

Magnetic drug targeting during Caputo fractionalized blood flow through permeable vessel

Heat and Mass Transfer Effects on MHD Casson Fluid Flow of Blood in Stretching Permeable Vessel

Heat and Mass Transfer Effects on MHD Casson Fluid Flow of Blood in Stretching Permeable Vessel

Molecular Targets for Nonhormonal Treatment Based on a Multistep Process of Adenomyosis Development.

Adenomyosis is an estrogen-dependent gynecologic disease characterized by the presence of endometrial tissue within the myometrium. Adenomyosis presents with abnormal uterine bleeding, pelvic pains, and infertility. This review aimed to investigate the major estrogen downstream effectors involved in the process of adenomyosis development and their potential use for nonhormonal treatment. A literature search was performed for preclinical and clinical studies published between January 2010 and November 2021 in the PubMed and Google Scholar databases using a combination of specific terms. Adenomyosis presents with a wide spectrum of clinical manifestations from asymptomatic to severe through a complex process involving a series of molecular changes associated with inflammation, invasion, angiogenesis, and fibrosis. Adenomyosis may develop through a multistep process, including the acquisition of (epi)genetic mutations, tissue injury caused at the endometrial-myometrial interface, inside-to-outside invasion (from the endometrial side into the uterine wall), or outside-to-inside invasion (from the serosal side into the uterine wall), and epithelial-mesenchymal transition, tissue repair or remodeling in the myometrium. These processes can be regulated by increased estrogen biosynthesis and progesterone resistance. The expression of estrogen downstream effectors associated with persistent inflammation, fragile and more permeable vessel formation, and tissue injury and remodeling may be correlated with dysmenorrhea, heavy menstrual bleeding, and infertility, respectively. Key estrogen downstream targets (e.g., WNT/β-catenin, transforming growth factor-β, and nuclear factor-κB) may serve as hub genes. We reviewed the molecular mechanisms underlying the development of adenomyosis and summarized potential nonhormonal therapies.

Magnetic drug targeting during Caputo-Fabrizio fractionalized blood flow through a permeable vessel.

Nanoparticle-based drug targeting is an important platform for the treatment of cardiovascular disorders. Magnetic drug targeting is more significant as it is a noninvasive procedure and biocompatible. The present problem aims to understand magnetic drug delivery to a specific location in a permeable blood vessel under the vibration and magnetic environment. Caputo-Fabrizio fractional-order time derivatives are used in the governing equations. The momentum equations are solved analytically and presented in the form of Lorenzo-Hartley and Robotonov-Hartley functions and convolution of the Laplace transform. Convolution integrations are solved by using the numerical integration technique. The Fourth order Runge-Kutta method (RK4) is used to solve the force balance equation. The influence of pertinent parameters such as Reynolds number, pulsatile frequency, magnetic field strength, Darcy number and fractional-order parameters are presented through graphs. It is observed that increasing Reynolds number results in decreasing the tendency of the drug to capture near the tumor site, whereas the pulsatile frequency presents an opposite phenomenon. Increasing the magnetic field strength and Darcy number boosts the capture efficiency of drug particles near the tumor site. The short memory effect efficiently captures the magnetic drug carriers to a specific location under the action of suitable magnetic field strength.

Effect of Thermal Radiation and Chemical Reaction on MHD Flow of Blood in Stretching Permeable Vessel

This paper focuses on the theoretical analysis of blood flow in the presence of thermal radiation and chemical reaction under the influence of time dependent magnetic field intensity. Unsteady non linear partial differential equations of blood flow consider time dependent stretching velocity, the energy equation also accounts time dependent temperature of vessel wall and the concentration equation includes the time dependent blood concentration. The governing non linear partial differential equations of motion, energy and concentration are converted into ordinary differential equations using similarity transformations solved numerically by applying ode45. The effect of physical parameters, viz., the permeability parameter, unsteadiness parameter, Prandtl number, Hartmann number, thermal radiation parameter, chemical reaction parameter and Schmidt number on flow variables, viz., velocity of blood flow in vessel, temperature and concentration of blood, has been analyzed and discussed graphically. From the simulation study the following important results are obtained: velocity of blood flow increases with the increment of both permeability and unsteadiness parameter. The temperature of blood increases at the vessel wall as the Prandtl number and Hartmann number increase. Concentration of blood decreases as time dependent chemical reaction parameter and Schmidt number increases.

A moderate thermal dose is sufficient for effective free and TSL based thermochemotherapy.

Hyperthermia, i.e. heating the tumor to a temperature of 40-43°C is considered by many a valuable treatment to sensitize tumor cells to radiotherapy and chemotherapy. In recent randomized trials the great potential of adding hyperthermia to chemotherapy was demonstrated for treatment of high risk soft tissue sarcoma: +11.4% 5yrs. overall survival (OS) and for ovarian cancer with peritoneal involvement nearly +12months OS gain. As a result interest in combining chemotherapy with hyperthermia, i.e. thermochemotherapy, is growing. Extensive biological research has revealed that hyperthermia causes multiple effects, from direct cell kill to improved oxygenation, whereby each effect has a specific temperature range. Thermal sensitization of the tumor cell for chemotherapy occurs for many drugs at temperatures ranging from 40 to 42°C with little additional increase of sensitization at higher temperatures. Increasing perfusion/oxygenation and increased extravasation are two other important hyperthermia induced mechanisms. The combination of free drug and hyperthermia has not been found to increase tumor drug concentration. Hence, enhanced effectiveness of free drug will depend on the thermal sensitization of the tumor cells for the applied drug. In contrast to free drugs, experimental animal studies combining hyperthermia and thermo-sensitive liposomal (TSL) drugs delivery have demonstrated to result in a substantial increase of the drug concentration in the tumor. For TSL based chemotherapy, hyperthermia is critical to both increase perfusion and extravasation as well as to trigger TSL drug release, whereby the temperature controlled induction of a local high drug concentration in a highly permeable vessel is driving the enhanced drug uptake in the tumor. Increased drug concentrations up to 26 times have been reported in rodents. Good control of the tissue temperature is required to keep temperatures below 43°C to prevent vascular stasis. Further, careful timing of the drug application relative to the start of heating is required to benefit optimal from the combined treatment. From the available experimental data it follows that irrespective whether chemotherapy is applied as free drug or using a thermal sensitive liposomal carrier, the optimal thermal dose for thermochemotherapy should be 40-42°C for 30-60min, i.e. equivalent to a CEM43 of 1-15min. Timing is critical: most free drug should be applied simultaneous with heating, whereas TSL drugs should be applied 20-30min after the start of hyperthermia.

Stationary flow of a viscous liquid in a flat channel with permeable walls

The article considers the problem of stationary blood flow in vessels with permeable walls. To determine the hydraulic resistance in an arterial vessel, the blood is considered Newtonian viscous fluid, and the flow is stationary. When solving problems, formulas were obtained for determining the corresponding hydrodynamic parameters, such as speed, fluid flow rate and pressure gradient. The impedance method is determined by the hydraulic resistance. In a stationary flow, the hydraulic resistance in the permeable vessel substantially depends on the permeability coefficient: with an increase in this coefficient, it decreases.

Bioinspired Microfibers with Embedded Perfusable Helical Channels.

Materials with microchannels have attracted increasing attention due to their promising perfusability and biomimetic geometry. However, the fabrication of microfibers with more geometrically complex channels in the micro- or nanoscale remains a big challenge. Here, a novel method for generating scalable microfibers with consecutive embedded helical channels is presented using an easily made coaxial microfluidic device. The characteristics of the helical channel can be accurately controlled by simply adjusting the flow rate ratio of the fluids. The mechanism of the helix formation process is theorized with newly proposed heterogenerated rope-coil effect, which enhances the tunability of helical patterns and promotes the comprehension of this abnormal phenomenon. Based on this effect, microfibers with embedded Janus channels and even double helical channels are generated in situ by changing the design of the device. The uniqueness and potential applications of these tubular microfibers are also demonstrated by biomimetic supercoiling structures as well as the perfusable and permeable spiral vessel.

EFFECT OF PERMEABLE VESSEL CLOSURE AND GELLING AGENT ON REDUCTION OF HYPERHYDRICITY IN IN VITRO CULTURE OF CARNATION

Hyperhydricity, an abnormal morphological appearance and physiological<br />function, is an important problem in carnation tissue culture. The problem causes premature flowering, high occurrence of abnormal shoots, difficulty in transferring hyperhydric plantlets to soil, and low survival rate of plantlets. High relative humidity and the water potential are considered as the key factors involved in the abnormality. Furthermore, permeable culture vessel and gelling agent were assured to be high potential treatment to eliminate it. Objective of this research was to reduce hyperhydricity in regenerants of carnation using different permeable vessel closures and gelling agents and to assess the multiplication and acclimatization abilities of recovered shoots. Experiment was arranged in randomized complete block design with four replications. First factor was different types of closure, i.e. cotton wool, plastic wrap, parafilm and aluminium foil, while second one was gelling agents, i.e. bacto agar, phytagel, swallow agar, and Type 900 agar. The recovered shoots were then multiplied, rooted, and acclimatized. The results showed that hyperhydricity was successfully reduced by applying permeable closure (cotton wool and plastic wrap) in combination with Type 900 agar. The combination of plastic wrap and Type 900 agar was the most appropriate treatment in reducing hyperhydricity and producing good quality shoots. The treatment reduced the problem down to 23% of total condition of hyperhydricity (100%) and increased leaf chlorophyll content from 0.0883 to 0.1288 mg mg-1. The plastic wrap was easily applied and cheaper material compared to cotton wool. The recovered shoots were able to produce 1-3 healthy axillary shoots and easily rooted on half-strength MS. The recovered plantlets were simply acclimatized with survival rate up to 100% on kossas peat + soil (1:1, v/v) and flowered 4-5 months after acclimatization with decreasing in number and size of flower.

EFFECT OF PERMEABLE VESSEL CLOSURE AND GELLING AGENT ON REDUCTION OF HYPERHYDRICITY IN IN VITRO CULTURE OF CARNATION

Hyperhydricity, an abnormal morphological appearance and physiological<br />function, is an important problem in carnation tissue culture. The problem causes premature flowering, high occurrence of abnormal shoots, difficulty in transferring hyperhydric plantlets to soil, and low survival rate of plantlets. High relative humidity and the water potential are considered as the key factors involved in the abnormality. Furthermore, permeable culture vessel and gelling agent were assured to be high potential treatment to eliminate it. Objective of this research was to reduce hyperhydricity in regenerants of carnation using different permeable vessel closures and gelling agents and to assess the multiplication and acclimatization abilities of recovered shoots. Experiment was arranged in randomized complete block design with four replications. First factor was different types of closure, i.e. cotton wool, plastic wrap, parafilm and aluminium foil, while second one was gelling agents, i.e. bacto agar, phytagel, swallow agar, and Type 900 agar. The recovered shoots were then multiplied, rooted, and acclimatized. The results showed that hyperhydricity was successfully reduced by applying permeable closure (cotton wool and plastic wrap) in combination with Type 900 agar. The<br />combination of plastic wrap and Type 900 agar was the most appropriate treatment in reducing hyperhydricity and producing good quality shoots. The treatment reduced the problem down to 23% of total condition of hyperhydricity (100%) and increased leaf chlorophyll content from 0.0883 to 0.1288 mg mg-1. The plastic wrap was easily applied and cheaper material compared to cotton wool. The recovered shoots were able to produce 1-3 healthy axillary shoots and easily rooted on half-strength MS. The recovered plantlets were simply acclimatized with survival rate up<br />to 100% on kossas peat + soil (1:1, v/v) and flowered 4-5 months after acclimatization with decreasing in number and size of flower.

Effect of heat transfer on unsteady MHD flow of blood in a permeable vessel in the presence of non-uniform heat source

This paper presents a theoretical analysis of blood flow and heat transfer in a permeable vessel in the presence of an external magnetic field. The unsteadiness in the coupled flow and temperature fields is considered to be caused due to the time-dependent stretching velocity and the surface temperature of the vessel. The non-uniform heat source/sink effect on blood flow and heat transfer is taken into account. This study is of potential value in the clinical treatment of cardiovascular disorders accompanied by accelerated circulation. The problem is treated mathematically by reducing it to a system of coupled nonlinear differential equations, which have been solved by using similarity transformation and boundary layer approximation. The resulting nonlinear coupled ordinary differential equations are solved numerically by using an implicit finite difference scheme. Computational results are obtained for the velocity, temperature, the skin-friction coefficient and the rate of heat transfer in the vessel. The estimated results are compared with another analytical study reported earlier in scientific literatures. The present study reveals that the heat transfer rate is enhanced as the value of the unsteadiness parameter increases, but it reduces as the space-dependence parameter for heat source/sink increases.

In-Depth Proteomics Identifies a Role for Autophagy in Controlling Reactive Oxygen Species Mediated Endothelial Permeability

Endothelial cells (ECs) form the inner layer of blood vessels and physically separate the blood from the surrounding tissue. To support tissues with nutrients and oxygen, the endothelial monolayer is semipermeable. When EC permeability is altered, blood vessels are not functional, and this is associated with disease. A comprehensive knowledge of the mechanisms regulating EC permeability is key in developing strategies to target this mechanism in pathologies. Here we have used an in vitro model of human umbilical vein endothelial cells mimicking the formation of a physiologically permeable vessel and performed time-resolved in-depth molecular profiling using stable isotope labeling by amino acids in cell culture mass spectrometry (MS)-proteomics. Autophagy is induced when ECs are assembled into a physiologically permeable monolayer. By using siRNA and drug treatment to block autophagy in combination with functional assays and MS proteomics, we show that ECs require autophagy flux to maintain intracellular reactive oxygen species levels, and this is required to maintain the physiological permeability of the cells.

Hybrid Common Femoral Artery Surgical Revascularization Associated to Endovascular Femoropopliteal Recanalization in High-Risk (ASA 3 - 4) Patients: A Seven-Year Period Institutional Experience

Objective: The present study was conceived to analyze the clinical benefit of hybrid interventions with surgical common femoral artery (CFA) reconstruction coupled to superficial femoral/popliteal endovascular recanalization for severe infrainguinal multilevel occlusive disease in high-risk ASA Class 3 - 4 patients. Material and Methods: From August 2008 until May 2015, a series of 143 hybrid infrainguinal interventions in 124 ASA Class 3 - 4 patients were performed in our department for Rutherford category 2 - 6 ischemic presentations. Patient demographics, specific risk factors, technical characteristics and patency results were retrospectively examined during a mean 36.8 months of follow-up. In a majority of 94 limbs (65%), the endovascular stage of interventions focused on long (>15 cm) femoropopliteal occlusions in parallel to regular CFA surgical revascularization. Two or three runoff tibial trunks were evinced in 84% cases, while one or none permeable vessel was found in 23 (16%) limbs. Results: Inasmuch surgical approach was successful in all cases, the endovascular stage was technically profitable in 134 (93%) cases. The ABI posto-peratively improved (>1.5) in 73% of cases, while clinical presentation gained at least one Rutherford category in 89% limbs. The mean hospital stay was 6.1 days (3 - 12 days) whereas the 30-day mortality rate in this homogeneous “high-risk” group of patients was 3.2%. Global risk factors alike age (>70 years/p = 0.0005), smoking ((p = 0.0170) and female gender (p = 0.0111), together with CTOs length (>15 cm/(p = 0.0470), severe calcifications (p = 0.0001), poor tibial runoff (p = 0.0001), TASC “C” and “D” lesions (p = 0.360 and (p = 0.0394), the stent number ((n = 3) and length (>6 cm) ((p = 0.0039 and (p = 0.0003) and the initial ABI scoring ((p = 0.0051) showed statistical negative influence on primary patency. Conclusion: Hybrid infrainguinal revascularization may afford useful results in selected ASA “high risk” patients, owning low invasiveness, reproducibility and acceptable patency in return to punctual postoperative surveillance.

A completely noninvasive method of dissolved oxygen monitoring in disposable small‐scale cell culture vessels based on diffusion through permeable vessel walls

Disposable cell culture vessels are extensively used at small scales for process optimization and validation, but they lack monitoring capabilities. Optical sensors that can be easily adapted for use in small-scale vessels are commercially available for pH, dissolved oxygen (DO), and dissolved carbon dioxide (DCO2 ). However, their use has been limited due to the contamination and compatibility issues. We have developed a novel solution to these problems for DO monitoring. Oxygen diffusion through permeable vessel wall can be exploited for noninvasive monitoring. An optical oxygen sensor can be placed outside the oxygen permeable vessel wall thereby allowing oxygen diffusing through the vessel wall to be detected by the sensor. This way the sensor stays separate from the cell culture and there are no concerns about contaminants or leachants. Here we implement this method for two cell culture devices: polystyrene-made T-75 tissue culture flask and fluorinated ethylene propylene (FEP)-made Vuelife(®) cell culture bag. Additionally, mammalian and microbial cell cultures were performed in Vuelife(®) cell culture bags, proving that a sensor placed outside can be used to track changes in cell cultures. This approach toward noninvasive monitoring will help in integrating cell culture vessels with sensors in a seamless manner.

Coupled finite difference and boundary element methods for fluid flow through a vessel with multibranches in tumours

A mathematical model and a numerical solution procedure are developed to simulate flow field through a 3D permeable vessel with multibranches embedded in a solid tumour. The model is based on Poisseuille's law for the description of the flow through the vessels, Darcy's law for the fluid field inside the tumour interstitium, and Starling's law for the flux transmitted across the vascular walls. The solution procedure is based on a coupled method, in which the finite difference method is used for the flow in the vessels and the boundary element method is used for the flow in the tumour. When vessels meet each other at a junction, the pressure continuity and mass conservation are imposed at the junction. Three typical representative structures within the tumour vasculature, symmetrical dichotomous branching, asymmetrical bifurcation with uneven radius of daughter vessels and trifurcation, are investigated in detail as case studies. These results have demonstrated the features of tumour flow environment by the pressure distributions and flow velocity field.

The Distribution of Fluid Shear Stresses in Capillary Sprouts

Fluid shear stress has been implicated as a regulator of sprouting angiogenesis. However, whether endothelial cells within capillary sprouts in vivo experience physiologically relevant shear stresses remains unclear. The objective of our study is to estimate the shear stress distribution along the length of a capillary sprout through computational modeling of blood flow in a blind-ended channel branching off a host vessel. In this model, we use sprout geometries typical for the rat mesenteric microvasculature and consider three types of boundary conditions: (1) a non-permeable vessel wall, (2) a uniformly permeable vessel wall, and (3) a non-permeable vessel wall with open slots (representative of endothelial clefts). Our numerical simulation predicts that for each boundary condition a local maximum shear stress (13.9, 8.9, and 13.3 dyne cm−2, respectively) occurs at the entrance of a 50 μm long, 6 μm diameter sprout branching at 90° off of a 11 μm diameter host vessel. The shear stress dropped below 0.2 dyne cm−2, a threshold for endothelial cell activation, within 4.1 μm of the entrance for the non-permeable wall case and 4.2 μm for the uniformly permeable wall case. Shear stress magnitudes within the sprout were above 0.2 dyne cm−2 for longer sprout scenarios and peaked at 5.9 dyne cm−2 at endothelial cell clefts. These results provide a first estimate of relative fluid shear stress magnitudes along a capillary sprout and highlight the importance of investigating endothelial cell responses to flow conditions during angiogenesis in tumors and other altered microenvironments.

TRANSLATIONAL IMPACT

In patients with locally advanced solid tumors, the first-line treatment is often neo-adjuvant or pre-operative chemotherapy, which helps shrink tumors before surgery, allowing for more conservative surgical approaches and reducing the potential for developing systemic disease. However, despite aggressive chemotherapy, long-term survival for many patients remains poor, in part owing to limitations with the targeting and accumulation of cytotoxic drugs in tumor tissue.The vasculature of solid tumors is abnormal, both in terms of vessel architecture and the dynamics of blood flow. Permeable heterogeneous vessel walls allow the leakage of proteins and fluid that, coupled with the inefficiency of lymphatic drainage, could be exploited to develop novel, enhanced drug delivery strategies that are therapeutically selective and improve clinical outcome.This work describes a previously unappreciated role for transforming growth factor beta (TGFβ) in regulating vascular stability and vessel permeability in solid tumors. Using mouse models, the authors demonstrate an endogenous pathway that regulates normal vascular permeability, which is controlled by perivascular collagen, the metalloproteinase enzyme MMP14, and TGFβ. In wild-type mice, inhibitors of either MMP14 or TGFβ signaling induce blood vessel permeability. Conversely, enhanced MMP14 or TGFβ activity in the mouse epidermis decreases leakage across cutaneous vessels. This pathway remains functional during tumor progression, as acute blockade of either MMP14 or TGFβ signaling transiently alters vessel stability, ‘opening’ vascular beds and promoting intravenous delivery of high molecular weight compounds to the tumor.The delivery of standard therapeutic agents or diagnostic molecular imaging agents to tumor tissue may be enhanced by transient blockade of the TGFβ pathway. If so, this could advance disease therapy and/or diagnostic imaging, not only in cancer medicine, but also in fibrotic disorders such as scleroderma and kidney failure.

Magnetic Drug Targeting in the Permeable Blood Vessel—The Effect of Blood Rheology

The present investigation deals with finding the trajectories of the drug dosed magnetic carrier particle in a microvessel, which is subjected to the external magnetic field. We consider the physical model that was given in the work of Furlani and Furlani (2007, “A Model for Predicting Magnetic Targeting of Multifunctional Particles in the Microvasculature,” J. Magn. Magn. Mater., 312, pp. 187–193), but deviating by taking the non-Newtonian fluid model for the blood in the permeable microvessel. Both the Herschel–Bulkley fluid and Casson models are considered to analyze the present problem. The expression for the fluid velocity in the permeable microvessel is obtained using the analogy given by Decuzzi et al. (2006, “The Effective Dispersion of Nanovectors Within the Tumor Microvasculature,” Ann. Biomed. Eng., 34, pp. 633–641) first. Then the expression for the fluidic force for the carrier particle traversing in the non-Newtonian fluid is obtained. Several factors that influence the magnetic targeting of the carrier particles in the microvasculature, such as the permeability of the inner wall, size of the carrier particle, the volume fraction of embedded nanoparticles, and the diameter of the microvessel are considered in the present problem. The trajectories of the carrier particles are found in both invasive and noninvasive targeting systems. A comparison is made between the trajectories in these cases in both the Casson and Herschel–Bulkley fluid models. The present results for the permeable microvessel are compared with the impermeable inner wall trajectories given by Shaw et al. (2010, “Effect of Non-Newtonian Characteristics of Blood on Magnetic Targeting in the Impermeable Micro Vessel,” J. Magn. Magn. Mater., 322, pp. 1037–1043). Also, a prediction of the capture of therapeutic magnetic nanoparticle in the human permeable microvasculature is made for different radii and volume fractions in both the invasive and noninvasive cases.

Effect of sealed and vented gaseous microenvironments on the hyperhydricity of potato shoots in vitro

Different types of ventilation of the culture vessel with and without the ethylene inhibitor KMnO 4 in the culture media were investigated in terms of their effect on hyperhydricity of in vitro cultured potato shoots. The shoots grown in a completely sealed vessel (CSV) were severely vitrified, but the shoots cultured in a gas permeable vessel (GPV) showed normal shoot proliferation. Dry weight percentage and chlorophyll content were lower in the vitrified shoots than in the normal shoots. The levels of ethylene and CO 2 were significantly higher in the CSV, but little ethylene was detected in the GPV system. When an ethylene scrubber (perlite treated with KMnO 4) was applied to the CSV, the shoots exhibited normal growth without any sign of vitrification. In addition, no ethylene was found in CSV with the ethylene scrubber (CSVE). These results indicate that hyperhydricity is related to the ethylene accumulated in the culture vessel during the in vitro culture of potato shoots. It is suggested to use CSVE to reduce the ethylene level and overcome hyperhydricity.